• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.14-14
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• 2019

참삿갓사초는 전국에 분포하는 여러해살이풀로 사료용, 사방용 소재로 사용되며 조경용, 관상용 지피식물로 사용되기도 한다. 참삿갓사초의 대량증식을 위해 가장 효과적인 파종 시기를 추정하기 위해 생장상을 이용해 인공적인 온도조건을 주고 참삿갓사초 종자의 발아율을 조사하였다. 실험을 위해 온도를 항온조건(30, 25, 20, 15, $5^{\circ}C$)과 변온조건(15/30, 20/10, $25/15^{\circ}C$) 총 8가지 조건으로 설정하여 발아율을 매일 측정하였다. 광조건은 명/암을 매일 12시간씩 반복하였다. 항온조건에서 발아율은 $20^{\circ}C$가 $17{\pm}7%$로 가장 우수하였고, $25^{\circ}C$ ($12{\pm}2.8%$), $30^{\circ}C$ ($3{\pm}1.9%$) 순으로 나타났으며, $15^{\circ}C$, $5^{\circ}C$에서는 발아하지 않았다. 그러나 $20^{\circ}C$와 $25^{\circ}C$조건에서 통계적인 유의차는 발생되지 않았다. 한편, 변온조건에서의 발아율은 $15/30^{\circ}C$가 $91{\pm}3.8%$로 가장 우수하였고, $25/15^{\circ}C$ ($89{\pm}4.1%$), $20/10^{\circ}C$ ($69{\pm}3.4%$) 순으로 나타났다. 그러나 $15/30^{\circ}C$와 $25/15^{\circ}C$조건에서 통계적인 유의차는 발생되지 않았다. 따라서 참삿갓사초의 종자발아를 위해서는 변온이 필요하며, $25^{\circ}C$ 이상의 온도가 필요하다. 이러한 결과를 바탕으로 가장 효과적인 파종 시기를 추정할 때, 낮-밤 $10^{\circ}C$ 이상의 기온차가 있는 초여름~늦여름 사이가 가장 적절할 것으로 판단된다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.17-17
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• 2019

진퍼리사초는 한반도 중부 이북에 분포하고, 군락 자생지는 강원도 동해안 석호 주변 모래땅 1곳인 것으로 보고되고 있다. 따라서 본 연구는 진퍼리사초 현지외보전을 위한 대량증식 연구의 일환으로 가장 효과적인 파종 시기를 구명하고자 수행하였다. 종자의 최적 발아조건을 찾기 위해 배양상 온도조건을 항온(30, 25, 20, 15, $5^{\circ}C$)과 변온(15/30, 20/10, $25/15^{\circ}C$) 총 8가지 조건으로 설정하여 발아율을 매일 측정하였다. 광조건은 명/암을 매일 12시간씩 반복하였으며 모든 실험은 국립백두대간수목원 발아실험실의 생장상에서 진행하였다. 실험결과, 항온조건에서 발아율은 $30^{\circ}C$에서 $39{\pm}7.5%$로 가장 높게 나타났고, $20^{\circ}C$ ($28{\pm}4.3%$), $25^{\circ}C$ ($24{\pm}3.7%$) 순으로 나타났으며, $15^{\circ}C$, $5^{\circ}C$에서는 발아하지 않았다. 한편, 변온조건에서의 발아율은 $25/15^{\circ}C$에서 $60{\pm}5.9%$로 가장 높게 나타났고, $15/30^{\circ}C$ ($43{\pm}4.1%$), $20/10^{\circ}C$ ($26{\pm}3.8%$) 순으로 나타났다. 따라서 진퍼리사초의 종자 발아는 항온보다 변온에서 잘 이루어졌으며, $25^{\circ}C$이상의 온도가 효과적인 것으로 나타났다. 이러한 결과를 바탕으로 가장 효과적인 파종 시기를 추정할 때, 낮 기온이 평균 $25^{\circ}C$이상이며, 일교차가 $10^{\circ}C$ 정도인 초여름이 가장 적절할 것으로 여겨진다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.18-18
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• 2019

층실사초는 경북 북부를 포함한 강원도 지역의 습기있는 음지에서 자생한다. 여러해살이풀로서 주료 사료용으로 사용되며 지피식물 등 정원소재로도 사용된다. 본 연구는 층실사초의 대량증식을 위해 가장 효과적인 파종 시기를 구명하고자 수행하였다. 종자의 최적 발아조건을 찾기 위해 배양상 온도조건을 항온조건(30, 25, 20, 15, $5^{\circ}C$)과 변온조건(15/30, 20/10, $25/15^{\circ}C$) 총 8가지 조건으로 설정하여 발아율을 매일 측정하였다. 광조건은 형광등을 이용한 명조건과 암조건을 매일 12시간씩 반복하였다. 실험결과, 항온조건에서 발아율은 $25^{\circ}C$에서 $36{\pm}6.3%$로 가장 높게 나타났고, $20^{\circ}C$ ($27{\pm}4.4%$), $30^{\circ}C$ ($24{\pm}1.6%$) 순으로 나타났으며, $15^{\circ}C$, $5^{\circ}C$에서는 발아하지 않았다. 한편, 변온조건에서의 발아율은 $25/15^{\circ}C$에서 $22{\pm}7.3%$, $15/30^{\circ}C$에서 $10{\pm}2.5%$ 발아하였고, $20/10^{\circ}C$에서는 발아하지 않았다. 따라서 층실사초 종자의 발아최적온도는 $25^{\circ}C$이고 변온보다 항온이 유리했으며, 이 이상의 온도에서는 오히려 발아율이 낮아지는 것으로 나타났다. 이러한 결과를 바탕으로 가장 효과적인 파종 시기를 추정할 때, 낮 기온 $25^{\circ}C$의 초여름에 실시하되, 밤 기온이 너무 내려가지 않도록 조절해준다면 보다 높은 발아율을 기대할 수 있을 것으로 여겨진다. 그러나 본 연구결과, 최고 발아율이 $36{\pm}6.3%$로 비교적 낮기 때문에 발아율 향상을 위한 추가적인 실험이 필요할 것이다.

• Korean Journal of Plant Resources
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• v.37 no.1
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• pp.79-86
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• 2024

This study was conducted to determine the conditions for breaking dormancy in the seeds of the North Korean plant resource, Trollius macropetalus, and to investigate its germination characteristics. The morphological characteristics and viability of T. macropetalus seeds were examined. Germination characteristics were investigated based on temperature conditions (15/6℃ & 25/15℃), cold stratification treatment, and GA₃ treatment. The results showed that cold stratification treatment had no effect on breaking the dormancy of T. macropetalus. GA₃ treatment significantly increased the germination rate of T. macropetalus and also improved the mean germination days and germination speed. However, compared to the 15/6℃ condition, the effectiveness of GA₃ treatment for T. macropetalus decreased under the 25/15℃ condition. It was determined that T. macropetalus have a nondeep-type morpho-physiological dormancy. The most effective conditions for breaking dormancy were found to be the 15/6℃ temperature condition and GA₃ treatment at 500 mg·L^-1. The findings of this study are expected to provide valuable information for the mass propagation of T. macropetalus, which has potential applications in landscaping and pharmaceutical materials.

• YAKHAK HOEJI
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• v.8 no.2
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• pp.45-47
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• 1964

Two new orotic acid derivatives orotyl-DL-alanine and orotyl-L-tyrosine were synthesized. They were obtained as high melting crystalline masses by condensing DL-alanine and L-tyrosine each with orotyl chloride in aqueous sodium hydroxide solution, followed by acidifying the reaction mixture.

• Journal of Biosystems Engineering
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• v.35 no.1
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• pp.46-52
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• 2010

The temperature of root zone was known as an important factor for the growth of crops and reduction of energy in greenhouse. The purpose of this study was to design the apposite inflow of calories per the unit area by comparison of temperature in the warmed and non-warmed soil. The energy needed for soil warming about pipe length showed the change of temperature on inflow and outflow as $2^{\circ}C{\sim}3^{\circ}C$(average $2.5^{\circ}C$). Therefore, the inflow per the unit hour was 3,450, 57,5 kcal/$h{\cdot}m^2$ on soil heating respectively. The non-warmed soil temperature in greenhouse made a difference by depth and it was partially affected inner temperature under 15 cm, but it was not above 15 cm. The soil temperature would be raised over $5^{\circ}C$ than non-warmed soil to increase effect of soil warming. Therefore, the inflow per the unit area that should be provided was about 100 kcal/$h{\cdot}m^2$.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.2
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• pp.187-192
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• 1994

This study was conducted to investigate varietal difference in viviparous germinability at different days after heading and temperature conditions. Three Japonica rice varieties, Sobaekbyeo, Odaebyeo and Hwaseongbyeo, and Sare type rice variety, Jakwangdo, showed higher viviparous germination rate from 20 days after heading, while a Tongil type rice, ]ungwonbyeo, and a Indica varieyt, IR-20, showed still low viviparous germination at 40 days after heading. Viviparous germination rate of rice varieties observed at 4 and 10 days after incubation was greately increased with increasing temperature from 15$^{\circ}C$ to 3$0^{\circ}C$ , and germination rate less than 5% was observed at 4 days after incubating at 15$^{\circ}C$ in all rice varieties teased. Viviparous germination pattern of rice varieties was divided into two types. Three Japonica and Sare type rice varieties showed higher and faster in viviparous germination than a Tongil type and Indica rice verieties. Viviparous germination was delayed under day /night fluctuation of incubation temperature compared with day /night fluctuation of incubation temperature compared with day /night constant condition at the same average temperature. The optimum incubation temperature and duration to evaluate viviparous germinability of rice varieties were 25/15$^{\circ}C$ and 6 days, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.17 no.4
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• pp.283-289
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• 1988

Lysine is known as a limiting amino acid in barley and easily inactivated by the browning reaction during processing or storage. The barley powders ground to 120 mesh in particle size were controlled at water activity of 0.44, 0.52, 0.65 and 0.75 by using saturated salt solutions and then stored at 35, 45 $55^{\circ}C$. Another portion of the sample of which the water activities were controlled as same above was stored at 35, 45, and $55^{\circ}C$ alternately with 7days interval. The reaction of available lysine loss in barley powders was found to be first order. The activation energies calculated from Arrhenius plot ranged $6.02{\sim}10.32Kcal/mole$, and $Q_10$ values were between 1.34 and 1.65. These kinetic parameters were used to predict the available lysine loss of barley powders under the fluctuating temperature storage The predicted shelf-life at various water activities tested was a little higher than the actual values.

• Journal of Biosystems Engineering
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• v.36 no.3
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• pp.211-216
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• 2011

According to the result of the first report and the second report of this study, it was expected that soil heating in a protected cultivation in winter season would affect the initial growth and development of fruit. Based on the result of previous study, we compared height, leaf number, leaf area, fruit weight, crop growth rate (CGR), features and quantity of cucumber for 3 months after planting between the soil heating group and the non-heating group. The result were summarized as follows: The height, leaf number, leaf area and fruit weight of cucumber in the soil heating group were 12.5%, 14.6%, 21.4% and 22.8% higher, respectively, compared to those of cucumber in the non-heating group. Although both the soil heating group and the non-heating group similarly showed an increasing pattern in CGR after transplanting, the soil heating group showed the increased CGR by 12.1% compared to that of the non-heating group. The quantity of cucumber in the soil heating group was about 26% higher than that of the non-heating group. It is assumed that the activation of initial growth and development of fruit in the heating group resulted in the increase of quantity.

• Korean Journal of Environmental Agriculture
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• v.37 no.4
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• pp.324-332
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• 2018

BACKGROUND: Insects are ectothermic organisms in terrestrial ecosystems and play various roles such as controlling plant biomass and maintaining species diversity. Because insects are ectothermic, their physiological responses are very sensitive to environmental temperature which determines survival and distribution of insect population and that affects climate change. This study aimed to identification of genes contributing to fitness under high temperature. METHODS AND RESULTS: To identify genes contributing to fitness under high temperature, the transcriptomes of fat body in Plutella xyostella larva have been analyzed via next generation sequencing. From the fat body transcriptomes, structure-related proteins, heat shock proteins, antioxidant enzymes and detoxification proteins were identified. Genes encoding proteins such as structural proteins (cuticular proteins, chitin synthase and actin), stress-related protein (cytochrome P450), heat shock protein and antioxidant enzyme (catalase) were up-regulated at high temperature. In contrast expression of glutathione S transferase was down-regulated. CONCLUSION: Identifications of temperature-specific up- or down-regulated genes can be useful for detecting temperature adaptation and understanding physiological responses in insect pests.