• Title/Summary/Keyword: 번식장애

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학술자료-II - 모기매개 바이러스에 의해 유발되는 번식장애 (소 츄잔병, 아까바네병, 아이노병)

  • Jeong, Seong-Dae
    • Journal of the korean veterinary medical association
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    • v.45 no.8
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    • pp.746-757
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    • 2009
  • 국립수의과학검역원과의 공동연구과제(소 아까바네병, 츄잔병, 아이노 바이러스혼합불활화 예방약 산업화)에 의해 실시한 시험을 통하여 소 아까바네병, 츄잔병, 아이노 바이러스병에 대한 예방약인(소 츄잔병, 아까바네병, 아이노병 불활화 혼합오일백신) 면역원성 시험 및 야외에서의 적용시험 등을 실시한 결과를 요약하면 다음과 같다. 1. 혼합오일백신의 기니픽에 대한 면역원성 시험에서 아까바네 바이러스, 아이노 바이러스 및 츄잔 바이러스에 대한 중화항체가는 시험백신을 3주 간격 2회 접종한 시험구가 무접종 대조구에 비하여 높게 나타났다. 2. 혼합오일백신의 야외농장 3곳의 육성우에 대한 면역원성 시험에서 시험백신을 2회 접종 후 아까바네 바이러스의 항체가는 평균 102배, 115배 및 134배이였으며, 아이노 바이러스의 항체가는 평균 8배, 24배 및 27배로 나타났고, 츄잔 바이러스의 경우에는 3곳의 농장에서 평균 30배, 46배 및 172배로 나타났다. 3. 야외농장 2곳에서 혼합오일백신을 임신우에 2회 접종하였을 때, 중화항체가는 아까바네 바이러스가 평균 64배와 93배를 나타내었고, 아이노 바이러스의 중화항체가는 평균 70배와 96배, 츄잔 바이러스의 중화항체가는 평균 20배와 20배로 나타났다. 4. 혼합오일백신은 임신우에 대한 공격접종시험에서 시험백신을 3주 간격으로 2회 접종 후 3주째의 중화항체가는 아까바네 바이러스가 128$\sim$256배이었고, 아이노 바이러스는 64$\sim$256배로 나타났고, 츄잔 바이러스는 8$\sim$32배이었다. 공격 접종 후 부검 전의 중화항체가는 아까바네 바이러스가 16$\sim$128배로 나타났고, 아이노 바이러스는 32$\sim$128배였으며, 츄잔 바이러스는 4$\sim$16배였다. 혼합오일백신을 임신우에 3주 간격 2회 접종한 뒤 공격접종하여 채혈한 혈청의 면역원성이 무접종 대조군에 비하여 방어효과가 우수하였다. 5. 3롯트의 시험백신을 선정하여 2$\sim$7$^{\circ}C$의 냉암소에 보존하면서 보존 기간별(제조당시, 6개월, 12개월, 15개월, 18개월)로 특성시험, 무균시험 및 안전시험을 실시한 결과, 전 보존기간에 마우스 및 기니픽에 대한 안전성이 인정되었으며, 보존기간별로 시험백신의 기니픽에 대한 면역원성 시험을 실시한 결과, 기니픽의 중화항체가는 15개월까지 지속되는 것으로 나타났다.

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Encephalomyocarditis virus infection in pigs associated with reproductive failure (번식장애를 수반한 돼지의 뇌심근염 바이러스 감염증)

  • Park, Nam-yong;Chung, Chi-young;Ri, Chang-yeong;Kee, Hye-young;Bae, Seong-yeol;Lee, Bong-ju;Ha, Yong-kong;Yoon, Seog-min;Jung, Byung-tack;Kim, Dong-sung
    • Korean Journal of Veterinary Research
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    • v.30 no.4
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    • pp.441-446
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    • 1990
  • Encephalomyocarditis virus infection in pigs, characterized by severe reproductive failure in sows and sudden death in less than 7 day-old-piglets, a period of several months from October to December of 1989, in Korea was occurred. The most outstanding gross lesions at necropsy were found in the heart which were consisted of pale, yellow brown necrotic foci of varying size in myocardium. In some cases, pulmonary edema and liver congestion were observed. Histopathology of the heart revealed the interstitial myocarditis, endocarditis and epicarditis with lymphocytic, plasmacytic and macrophage cell infiltration and, in some cases, calcification of the necrotic myocardial muscle fibers. In some cases of brain, the perivascular cuffing and glial nodules were observed. In the liver and the lacrimal gland varying degrees of multifocal necrosis were seen. The virus was isolated from the heart and the brain in a stillborn piglet and a mummified fetus. This outbreak represents the first case of encephalomyocarditis virus infection of pigs in Korea.

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Seroprevalence of Coxiella burnetii in cattle with reproductive disorders in eastern Gyeongbuk province, Korea (경북 동부지역 번식장애 소에서의 큐열 항체 보유율)

  • Ouh, In-Ohk;Seo, Min-Goo;Jang, Young-Sul;Kim, Sang-Yun;Kwak, Dong-Mi
    • Korean Journal of Veterinary Service
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    • v.36 no.4
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    • pp.249-254
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    • 2013
  • This study was done to determine the seroprevalence of Coxiella burnetii in cattle with reproductive disorders reared in eastern Gyeongbuk province. A total of 146 cattle blood samples from 83 farms in eastern Gyeongbuk province were collected in 2011~2012. Among them, 17 (11.6%) samples from 11 (13.3%) farms were seropositive for C. burnetii by ELISA. In dairy cattle with mastitis, 15 (38.5%) milk samples from 9 (42.9%) farms were seropositive among 39 milk samples from 21 farms. Among the seropositive farms, one farm with high seroprevalence was selected to assess the seroprevalence of C. burnetii in the bulk-tank milk (BTM) and blood samples of dairy cattle. The BTM sample from the farm was seropositive to C. burnetii and 6 (15%) out of 40 dairy cattle were seropositive. In cattle with reproductive disorders including infertility, abortion and stillbirth, 2 (1.9%) samples from 2 (3.2%) farms were positive among 107 samples collected from 62 farms. Among 2 positive samples, one of them is dariy cattle (100%) and the other is Korean cattle (0.9%) (P<0.001). This suggests that Korean cattle was relatively resistant to C. burnetii. Based on the data obtained, we identified association between C. burnetii shedding in milk and chronic subclinical mastitis in dairy cattle. Since seroprevalence of C. burnetii is relatively high in dairy cattle, further intensive studies on C. burnetii among the high risk milk farms and dairy cattle herds are needed to evaluate infection status.

Segmental Aplasia of the Right Uterine Horn in a Primiparous Holstein Cow (홀스타인 초산우에서 우측 자궁각의 부분무형성 일례)

  • Kim, Ill-Hwa;Kang, Hyun-Gu;Hur, Tai-Young;Lee, Hae-Rim;Mo, In-Pil
    • Journal of Veterinary Clinics
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    • v.29 no.5
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    • pp.427-430
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    • 2012
  • An infertile, three-year-old primiparous cow was diagnosed preliminarily with pyometra by rectal palpation at 180 days postpartum. Ultrasonography showed that the cranial portion of the right uterine horn was distended with fluid, while the base portion of the horn was missing. A dense band of tissue connected the cranial portion of the uterine horn to the uterine body. However, the left uterine horn was normal. Gross postmortem analysis of the reproductive organs confirmed the ultrasonographic observations. Histopathological observations also showed that dark red-brown mucus filled the thin-walled right uterine horn, where neither caruncles nor uterine glands were observed. Finally, this primiparous Holstein cow was diagnosed with segmental aplasia of the right uterine horn.

An Experimental Study on the Swimming Performance of Pale Chub(Zacco platypus) (피라미의 유영특성에 관한 실험적 연구)

  • Park, Seong-Yong;Kim, Seo-Jun;Lee, Seung-Hwi;Yoon, Byung-Man
    • Journal of Korea Water Resources Association
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    • v.41 no.4
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    • pp.423-432
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    • 2008
  • The local migration or movement behavior of fishes in streams are related to feeding, spawning, growing, dispersing, and refuging. The pale chub (Zacco platypus) is a dominant species that migrates locally and inhabits in river and stream in Korea. However, dams, weirs, culverts and other regulatory structures are physical barriers that limit fish movement and fragment habits and populations. If main stream and off-channel habitats are connected with culverts, they would restrict the small fish as pale chub movement due to the high flow velocities and low depths. But in Korea, there is no experimental study to evaluate the swimming performance of species in Korea. Therefore, it is difficult to proposed that design guidelines for pass fishes through culverts. The purpose of this experimental study is to evaluate the swimming performance of pale chubs. A series of swimming performance test has been used in both of the fixed velocity and the incremental velocity methods in an experimental flume. As a result, the critical swimming speed for pale chub(body length 8.9 cm) was found to be about 0.7 m/s. Therefore, the flow velocity for culvert design in the low flow condition should not be exceed the its swimming ability, especially 0.7 m/s for pale chubs(body length 8.9 cm). And the minimum depth for culvert design in the low flow condition should not be lower than the fish body height add a dorsal fin height.

참돔 노지종묘생산과 요각류 번식을 위한 적정 시비제

  • 방인철;박상용;권성민;신현웅
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2003.10a
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    • pp.3-63
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    • 2003
  • 우리나라 서해안의 폐염전 또는 대하양식장을 이용한 해산어 종묘생산이 조피볼락, 감성돔, 숭어 등을 대상으로 이루어지고 있다. 이렇게 생산된 종묘는 기형개체가 전혀 없는 건강 종묘이며, 생산 원가 또한 육상수조식에 비해 최고 5배정도 낮다. 한편 참돔은 육상수조에서 종묘생산시 기형률이 비교적 높게 나타나므로 노지를 이용한 종묘생산이 요구되고 있다. 현재 노지 종묘생산시 요각류 번식을 위한 시비제로는 암모니아, 인산염, 칼륨 등이 함유되어 있는 화학비료(요소, 복합비료)나 계분, 돈분, 우분 등의 유기질 비료가 주로 사용되고 있다. 그러나 화학비료를 사용시 급격한 수질변화로 요각류의 폐사가 발생하고, 매년 지속적인 생산량을 기대하기가 어려운 문제점이 있다. 따라서 적절한 시비제를 구명하기 위하여 유기질 비료(알팔파, 숙성계분)와 화학비료(요소, 복합)를 단독 또는 병행 사용하여 2개월간의 동ㆍ식물플랑크톤의 변동량과 치어의 생존율을 조사한 결과, 유기질 비료는 부화자어가 입식된 이후에도 연속하여 시비하여도 생존율에 영향을 미치지 않았으나, 화학비료의 경우 일정 농도 이상에서는 동ㆍ식물플랑크톤의 번식에 장애를 초래하였고 이에 따라 자어가 전량 폐사하였다. 실험군의 생존율은 육상수조에서 40%, 알팔파와 숙성계분을 시비한 실험군이 15%, 요소, 복합, 알팔파를 시비한 실험군이 10%, 요소, 복합, 숙성 계분을 시비한 실험군이 8%, 요소와 복합을 시비한 실험군은 0%를 나타내었다. 또한 기형률에 있어 노지 실험군은 0.7%로 매우 낮게 나타났으나, 육상수조 실험군은 61%에 해당하는 치어가 기형이었으며, 그 형태도 다양하게 나타났다.fer, Artemia sp., copepods, 부화 25일째부터는 copepods였다. 초기성장은 부화직후 전장 1.95 mm였던 것이 10일째는 2.96 mm, 20일째는 3.89 mm, 30일째는 12.9 mm, 50일째는 35.1 mm를 나타내었다. 그러나 말쥐치 자ㆍ치어가 성장함에 따라 발생하는 지속적인 폐사와 종묘의 수송시 발생하는 폐사를 줄일 수 있는 방법이 추후에 해결되어야 할 것으로 사료된다.8,000~215,000 마리의 자어를 낳아서 개체에 따라 큰 차이를 나타내었다. 1999년에는(어미 : 전장 20.7~25.5cm, 체중 205.5~396.4g) 1 회 출산때마다 7,000~50,000 마리의 자어를 출산하였다. 3. 출산간격. 다회 출산한 쏨뱅이 어미가 첫 번째 출산에서 다음 출산까지 소요되는 기간을 조사한 결과 13일에서 25일이 걸리는 것으로 나타났다. 특히 4회 출산 한 1998년 쏨뱅이 어미는 첫 번째 출산 후 19일만에 두 번째 출산이 이루어 졌고 각각 13 일과 14 일만에 세 번째, 네 번째 출산이 이루어져 총 46일 동안 4회에 거쳐 자어를 출산하였다. 4. 출산자어의 크기. 1997년 3회 출산한 쏨뱅이의 출산시기별 자어 크기를 조사한 결과 첫 번째 출산에서는 평균전장 4.1$\pm$0.07mm의 자어를 낳았으나 두 번째 출산에서 자어의 크기는 평균전장이 3.9$\pm$0.11mm, 세 번째 3.8$\pm$0.12mm로 점차 작은 경향을 나타내었다..27mm(평균 1.76mm, n=20) 그리고 Snout length 0.74~l.67mm(평

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Studies on Development of Breeding Technique to Increase HanWoo(Bos taurus coreanae) : II. Early Pregnancy Diagnosis Incidence of Reproductive Disorders (한우의 신속한 증식을 위한 번식기술 개발에 관한 연구 II. 조기 임신 진단법 및 번식장애 분포에 관한 연구)

  • Jang, G.;Son, C. H.;Lee, E. S.;Ryu, I. S.;Lee, K. N.;Lee, D. W.;Oh, M. H.;Oh, S. J.;Jung, K. K.;Choi, S. Y.;Roh, K. J.;Kim, S. C.;Lee, B. C.;Hwang, W. S.
    • Journal of Embryo Transfer
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    • v.16 no.1
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    • pp.7-14
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    • 2001
  • The aims of these study were to diagnose early pregnancy and reproductive disorders by using progesterone concentration and ultrasonography. The measurement of blood progesterone (P$_4$) concentration was conducted to diagnose pregnancy and to detect corpus luteum (CL) or evaluate disorder of CLs. As a result, the incidence rates of reproductive disorders were as follows : SH and EED (41.9%), inacitve ovaries (32.6%), follicullar cyst (9.3%), PCL (7.0%), endometritis (4.7%), pyometra (2.3%) and luteal cyst (2.3%). 61 Cows having P$_4$concentration 1.0 ng/ml(at the insemination) were increased to 1.0 ng/ml $\geq$ 6day after insemination. 50 cows among 61 cows were diagnosed pregnant. 8 cows among 13 HanWoos having P$_4$concentration 1.0 ng/ml at the insemination and 1.0 ng/mnl 6 day after insemination had non-ovulatory estrus and the others had P$_4$concentration 1.0 ng/ml at the insemination and 1.0 ng/ml $\geq$ 6 day after insemination, which was the error of estrus detection. All 13 cows were diagnosed non-pregnant. 47 cows diagnosed pregnant after insemination of P$_4$concentration 3.0 ng/ml were examined by ultrasonography at 30 day post-insemination. As a result, 41 cows were diagnosed pregnant (87.2%) but 14 cows having P$_4$concentration 3.0 ng/ml at 21 day after insemination was diagnosed to non-pregnancy. Calving intervals by surveying 100 cows were as follows 11~12 months (20%), 12~13 months (36%), 13~14 months (19%), 14 months $\geq$ (25%), respectively. In conclusion, hormone and ultrasonography help to detect reproductive disorders exactly and diagnose early pregnancy. This study suggest that diagnosis of early pregnancy and reproductive disorder by blood P$_4$concentration and ultrasonography improve reproduction management of HanWoo.

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Animal Model for Regeneration of Olfactory Sensory Neurons (후각신경세포의 손상 및 재생 연구모델의 융합연구)

  • Jeong, Yun-Mi;Park, Jong-Su;Kim, Cheol-Hee;You, Kwan-Hee
    • Journal of the Korea Convergence Society
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    • v.7 no.2
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    • pp.61-67
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    • 2016
  • The olfactory system is an important model for the study of neuronal degeneration and regeneration, including neuronal diseases. When the olfactory sensory neurons are damaged by nerve injury or are exposed to environmental factors, they degenerate and are replaced by regenerating neurons. To monitor neuronal degeneration in living animal, we established an olfactory-specific GFP transgenic zebrafish. The effects of Triton X-100 or sodium acetate on the olfactory system were examined. A significant decrease in the number of GFP-positive olfactory sensory neurons was observed after chemical lesion. We found a recovery of GFP-positive neurons by 2 days posttreatment. From these results, we expect that further studies of olfactory degeneration and regeneration using this transgenic zebrafish will provide important advances for the study of neuronal degeneration and regeneration.

Studies on the Number of Sperm Penetrated Oocytes and Survival Rate of Frozen-thawed Epididymal Dog Sperm (개 정소상체 정자의 난자내 침입율과 동결융해 후의 생존성에 관한 연구)

  • 박종민;김상근
    • Korean Journal of Animal Reproduction
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    • v.26 no.3
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    • pp.229-234
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    • 2002
  • This study was carried out to investigate the general characteristics such as concentration sperm motility and abnormality of sperm on the whole epididymal semen(EWS), RSP-S(removed seminal plasma by saline) and RSP-T(removed seminal plasma by tris-buffer) semen and survival rates after freezing on motility of whole and RSP-S and RSP-T semen and extender containing 2~8% glycerol, and ability of frozen-thawed sperm to penetrate homologous oocytes. 1. The concentration, motility and abnormality of epididymal WES, RSP-S and RSP-T sperm were 4.25 $\pm$ 0.25($\times$10$^{6}$ Cells/$m\ell$), 3.85$\pm$0.20($\times$10$^{6}$ Cells/$m\ell$), 4.05 $\pm$ 0.28($\times$10$^{6}$ Cells/$m\ell$), 50.55 $\pm$ 2.75%, 67.25 $\pm$ 2.55%, 78.75 $\pm$ 3.55 and 9.45 $\pm$ 2.25%, 37.75 $\pm$ 2.10%, 24.25 $\pm$ 1.55%, respectively. 2. The survival rates of slow and rapid frozen epididymal RSP-S and RSP-T sperm were 35.00 $\pm$ 2.35%, 45.50 $\pm$ 2.15% and 16.50 $\pm$ 3.55%, 22.55 $\pm$ 3.95%, respectively. The survival rate of epididymal WES and RSP-T sperm after freezing following dilution with tris-buffer containing 2~8% glycerol were 9.25 $\pm$ 1.55%~17.50 $\pm$ 2.50%. 3. The percentage of capacitated and acrosome-reacted sperm prier to culture for fresh and frozen -thawed epididymal RSP-T semen were 45.25 $\pm$ 5.75%, 7.06 $\pm$ 0.25%, 48.20 $\pm$ 6.80% and 13.00 $\pm$ 2.35%, 3.55 $\pm$ 0.85%, 15.50 $\pm$ 1.90%, respectively. The penetration rate the number of sperm per penetrated for fresh and frozen-thawed epididymal RSP-T sperm were 39.25 $\pm$ 4.72%, 34.24 $\pm$ 3.93% and 1.30 $\pm$ 0.33, 1.10 $\pm$ 0.50., respectively.

Porcine Circovirus Type 2 Infection in a Piglet Born from a Surrogate Mother (대리모에서 출생한 돼지에서 돼지 써코 바이러스 2형 감염)

  • Lee, Su-Hyung;Kang, Sang-Chul;Park, Jun-Won;Chung, Hee-Chun;Park, Bong-Kyun;Kim, Dae-Yong;Kim, Jae-Hoon
    • Journal of Veterinary Clinics
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    • v.31 no.3
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    • pp.258-261
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    • 2014
  • A 4-week-old male piglet being maintained in a research facility was found dead without any previous clinical signs. The piglet had been born from a surrogate mother after somatic nuclear transfer as part of a xenotransplantation study. Ovaries for nuclear transfer were obtained from a private farm outside the research facility. Histopathologically, multifocal to coalescing granulomatous myocarditis was observed in the heart, characterized by infiltration of lymphocytes, macrophages and multinucleated giant cells, and by myocardial necrosis and fibrosis. Lymphoid tissues showed marked lymphoid depletion with infiltration by histiocytes or giant cells. Immunohistochemistry showed PCV-2 antigens in necrotic myocytes, macrophages and multinucleated giant cells in the heart, as well as in macrophages and giant cells in lymphoid depleted areas of lymphoid tissues. Reproductive failure associated with PCV-2 in aborted or stillborn piglets is frequently characterized by myocarditis, and similar lesions were observed in this 4-week-old piglet with PCV-2 infection. The PCV-2 infection in this piglet may have been due to contamination or infection of an ovary from the pig farm.