• Korean Journal of Plant Resources
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• v.22 no.1
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• pp.42-48
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• 2009

This study was conducted to examine the difference in yield and size of enlarged rhizome according to the kinds of native white lotuses. Four white lotuses (Nelumbo nucifera) were collected from 4 different sites, Jeonbuk Jeonju ('Garam'), Jeonnam Muan ('Hoesan'), Chungnam Buyeo ('Ihnchuisa'), and Chungnam Cheongyang('Cheongyang'). Each enlarged rhizome was planted in an area of 3.3 $m^2$ on 10. April, 2007 and digged out on 19. February, 2008. The yield of enlarged rhizome was in the order of Hoesan (3.0 kg/$m^2$), Cheongyang (2.5 kg/$m^2$), Garam (2.0 kg/$m^2$) and Ihnchuisa (1.0 kg/$m^2$). The number of enlarged rhizomes was in the order of Ihnchuisa (4.9/$m^2$), Garam (4.3/$m^2$), Hoesan (4.2/$m^2$) and Cheongyang (4.1/$m^2$). Total number of nodes in the enlarged rhizomes were much more in the order of Hoesan (17.0/$m^2$), Ihnchuisa (16.0/$m^2$), Cheongyang (15.3/$m^2$) and Garam (14.1/$m^2$). Component ratio of enlarged rhizome length below 50cm for Ihnchuisa, Garam, Hoesan and Cheongyang were 76.3%, 53.0%, 38.2% and 40.3% respectively. Native white lotus had a longer enlarged rhizome in the first node than the other nodes, and their enlarged rhizome length and width became thin. And it became thick and short from the second node of enlarged rhizome. Accordingly the amount of enlarged rhizomes 'Hoesan' was extremely much than others.

• Journal of Life Science
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• v.20 no.8
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• pp.1241-1248
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• 2010

The solvent extracts of Nelumbo nucifera G. were investigated for antioxidant activities, whitening and anti-wrinkle effects to apply as a functional ingredient in cosmetic products. For their industrial application, the cosmetic products were also prepared with advanced formulation techniques such as W/O/W multiple emulsion. Total phenolic and flavonoids contents increased in Nelumbo nucifera G.-Leaf (NN-L). The electron donating ability of Nelumbo nucifera G.-Flower (NN-F) or Nelumbo nucifera G.-Leaf (NN-L) extracts were above 85% at a concentration of 500 ppm. The superoxide dismutase (SOD)-like activity of Nelumbo nucifera G. (NN-L) extracts was about 60% at a concentration of 1,000 ppm. The xanthine oxidase inhibitory effect of NN-L extract was higher than that of NN-F and NN-S extracts. The tyrosinase inhibitory effect, which is related to skin-whitening, was 36% in NN-F at 1,000 ppm. For anti-wrinkle effect, the elastase inhibition activity of NN-L was about 30% at 1,000 ppm. The results of stability test showed that W/O/W multiple emulsion (ME) containing Nelumbo nucifera G. extracts. The electron donating ability of the ME containing NN-F and NN-L were about 60% at a concentration of 100 ppm. The superoxide dismutase (SOD)-like activity of the ME containing NN-L was 30% at 1,000 ppm. The tyrosinase inhibitory effect, which is related to skin-whitening, was 34% in the ME containing NN-F at 1,000 ppm. In anti-wrinkle effect, the elastase inhibition activity of the ME containing NN-L was about 55% at 1,000 ppm.

• Journal of agriculture & life science
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• v.43 no.1
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• pp.43-50
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• 2009

To get basic data for the utilization of white and red lotus as a raw material in functional food, chemical components of its leaf and root were investigated. Leaf had the highest level of nitrogen free extract and root had the highest level of moisture. The mineral analysis showed K(380.44~1,516.34 mg/100g), Ca(36.67~1,323.92 mg/100g), P(84.02~473.13 mg/100g) and Na(57.73~304.07 mg/100g). The rich free sugars in white and red lotus were glucose(161.12~765.15 mg/100g) and frutose (100.57~901.12 mg/100g). Total amino acid contents in leaf and root of white lotus and leaf and root of red lotus were 6,385.57, 1,162.93, 6,003.01 and 1,242.20 mg/100g, respectively. Although the free amino acid compositions of white and red lotus were different, their major free amino acids were glutamic acid, phenylalanine arginine and tyrosine. The ascorbic acid were 248.65 and 20.99 mg/100g in leaf and root of white lotus, 156.92 and 9.32 mg/100g in leaf and root of red lotus. The leaf of white lotus exhibited the highest total phenolic contents at 24.33 mg/g.

• Korean Journal of Food Science and Technology
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• v.38 no.1
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• pp.114-120
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• 2006

Anti-diabetic effects of extracts and fractions of Sasa borealis (SB), white lotus roots (LR) and leaves (LL), and their mixture were determined in 3T3-L1 adipocytes and Min6 cells by investigating insulin-sensitizing activity and glucose-stimulated insulin secretion, respectively. SB, LR, LL, and mixture of SB, LR, and LL (3 : 2 : 3) were extracted using 70% ethanol, and m mixture extract was fractionated by XAD-4 column chromatography with serial mixture solvents of methanol and water. Fractional extractions were utilized for anti-diabetic effect assay. SB and LR extracts increased insulin-stimulated glucose uptake, but not as much as mixture of SB, LR, and LL. Significant insulin-sensitizing activities of 20 and 80% methanol fractions of SB, LR, and LL mixture extract were observed in 3T3-L1 adipocytes, giving 0.5 or $5\;{\mu}g/mL$ each fraction with 0.2 nM insulin to attain glucose uptake level similar to that attained by 10 nM insulin alone. Similar to pioglitazone, peroxisome proliferators-activated $receptor-{\gamma}\;(PPAR-{\gamma})$ agonist, 20 and 80% methanol fractions increased adipocytes by stimulating differentiation from fibroblasts and triglyceride synthesis. LL extract and 20, 60, and 80% methanol fractions of the mixture suppressed ${\alpha}-amylase$ activity, but did not modulate insulin secretion capacity of Min6 cells in both low and high glucose media. These data suggest 20 and 80% methanol tractions contain potential insulin sensitizers with functions similar to that of $PPAR-{\gamma}$ agonist. Crude extract of SB, LR, and LL mixture possibly improves glucose utilization by enhancing insulin-stimulated glucose uptake and inhibiting carbohydrate digestion without affecting insulin secretion in vivo.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.3 no.1
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• pp.19-26
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• 1970

During the summer of 1969, a series of experiments on the spawning and development of the eggs of grass carp and silver carp was performed with the following results, but the complete development of eggs Iras not accomplished: 1. The season of maturity for both species is estimated to be from the end of June to the beginning of August. 2. Pituitary glands for the use of inducing maturation should be obtained before the maturation season of its donor. 3. Silver carp as small as 40 cm in body length (48 cm in total length) with an age of 6 years were found to have reached maturity, and the number of eggs in the ovaries were from about 23 to 26 thousand for those with body lengths of 40-44.5 cm. 4. The conical-shaped net cloth incubator worked better than others, and each incubator was most effective when installed in parallel series to the water supply pipe with a valve for each incubator to control water flow. 5. During the egg development, if any cell or cells were displaced, the eggs eventually died. 6. The proper manipulation of brood fish before the egg-taking seems to be very Important, and close care should be paid to feeding them well and keeping them in a pond of proper water depth.

• Food Science and Preservation
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• v.17 no.1
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• pp.131-138
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• 2010

To obtain basic data on the use of lotus as a raw material in functional food, antioxidant and anticancer activities of the leaf and root were investigated. Total flavonoid and total phenolic contents, at 12.84 mg/g and 24.33 mg/g respectively, were higher in white lotus leaf (WLL) than in any other part of the plant. The radical-scavenging activity of different tissues of lotus, measured in the DPPH radical-scavenging assay, increased with higher concentrations of solvent fractions. The butanol fraction of white lotus leaf showed the highest DPPH radical-scavenging activity. The reducing power of fractions increased in a dose-dependent manner. The butanol fraction of WLL had the greatest reducing power, and showed strong antioxidant activity in the linoleic acid system, and high-level inhibition of tyrosinase. Fractions from lotus were also capable of scavenging nitrite, depending on the concentration of the fractions. Butanol fractions of the leaf of white and red lotus scavenged 95.61% and 92.15% of available nitrite, respectively, when used at 1 mg/mL concentrations. Butanol fractions from leaf of white and red lotus exhibited the strongest inhibitory effects on human lung and colon cancer cells.

• Proceedings of the Korean Institute of Resources Recycling Conference
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• 2003.10a
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• pp.81-84
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• 2003

TWO 협상의 결과로 농산물개방이 조만간 이루어지게 되면 쌀 생산을 중심으로 한 농업생산은 중국과 미국 등의 경쟁을 이기기가 상당히 어려워 질 것으로 생각된다. 또 쌀의 과잉생산으로 정부에서는 쌀 이외의 작물을 논에서 기르는 것을 장려하고 있다. 본 연구를 통해서 연재배 경제성과 재배된 연을 이용하여 다양한 차와 식품을 개발하는 것을 연구하였다. 먼저 연을 품종을 선별하였다. 본사에서 소유한 100여종의 연을 재배하여 식품으로 개발하기에 최적인 연을 선별하였다. 여러 가지 관능적인 미각 테스트와 생산량의 검정을 통해서 선별하였다. 일반적으로 많은 연들이 아린 기운이 많아서 식품으로 개발하기 어려웠다. 색깔이 진하면 진할 수록 아린 맛이 진하였다. 그러나 백련만은 맛도 우수하였고 생산성도 우수하였다. 식품으로 개발하기에 가장 적절한 시기를 조사하였다. 백련의 향은 시간이 갈수록 증가하다가 잎이 누렇게 변하면서 향이 떨어지기 시작하여 전 후가 가장 좋은 시기였다. 생산량은 시간이 갈수록 증가하였지만 이 추석 후에는 잎이 누렇게 변하기 시작하여 실제적으로 변하기 시작하여 식품으로 사용 가능한 생산량이 떨어 졌다. 수확된 제품을 연차와 연식품으로 개발하였다.

• Journal of Mushroom
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• v.7 no.4
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• pp.187-192
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• 2009

Tricholoma giganteum, belongs to Tricholomataceae of Tricholoma, is also well-known as the medicinal mushroom in Taiwan. "Backryon" was the first variety developed by intra-specific crossing in Korea. It was improved with hybridization between monokaryotic strain derived from MKACC50852 and MKACC 50853. The optimum temperature of mycelial growth and fruiting body development were $25{\sim}30^{\circ}C$ and $20{\sim}25^{\circ}C$, respectively. The color of fruitingbody was pure white and cap type was umbrella. It suggested that 'Backryon' was new commercial variety for small-sized cultivator during the summer season.

• Food Science of Animal Resources
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• v.31 no.4
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• pp.563-569
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• 2011

Heterocyclic amines (HCAs) are food mutagens and carcinogens that are found in cooked fish, meat, and protein-rich foods. This study examined the inhibitory effect of marinades containing a Nelumbo nucifera leaf fraction on HCA formation in cooked beef steak. As a result of the Ames assay, cooked beef marinated with the N. nucifera leaf butanol fraction (2.0 g) cooked at $190^{\circ}C$ showed a 61.5% reduction in overall mutagenicity. However, these data revealed no significant difference in mutagenicity in the ethanol, ethyl acetate, or water fractions. Formation of MeIQx (2-amino-3,8 dimethylimidazo[4,5-f]-quinoxalin) and PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]-pyridine) was inhibited 60.7-63.5%. Cooked beef marinated with the water fraction of the N. nucifera leaf significantly (p<0.05) reduced the formation of MeIQx, PhIP, and DiMeIQx (2-amino-3,4,8 trimethylimidazo[4,5-f]-quinoxaline) by 65.3, 67.6, and 73.9%, respectively. These results show that marinades containing the N. nucifera leaf fraction could be an alternative method for reducing HCA formation in cooked beef steak.

• The Journal of Internal Korean Medicine
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• v.30 no.4
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• pp.788-798
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• 2009

Objective : This study was undertaken to investigate effects of Opuntia ficus-indica (OP)extract on an asthma murine model. Methods : The total number of cells and eosinophils in BALF and the infiltration of inflammatory cells into lung tissues were determined by hematoxylin and eosin staining. Results : The number of OVA-induced total cells and eosinophils, a phenomenon of asthma, were decreased by treatment of the animals with OP extract (200 mg/kg) (respectively, p < 0.001 and p<0.01). Furthermore, we showed that OP extract reduced the increased immune cell infiltration induced by ovalbumin (p < 0.01). The levels of interleukin (IL)-4 in BAL fluid were measured by enzyme-linked immunosorbent assay, because the eosinophilia is associated with a T helper (Th) 2 response including IL-4. The level of OVA-induced IL-4 was decreased by OP extract in BAL fluid (p < 0.05). We investigated whether OP extract reduced nitrite (NO) production on lipopolysaccharide (LPS)-stimulated RAW 264.7 sells, because asthma is an inflammatory disease. The level of LPS-induced NO production was decreased by OP extract (50, 100 and 200 mg/ml) on RAW 264.7 cells (respectively, p < 0.05, p<0.01 and p<0.05). Conclusions : Our results indicate that OP extract has an inhibitory effect on lung eosinophilia of asthma and suggest that OP extract is a therapeutic candidate in the treatment of inflammatory disease, including asthma.