• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.46-146
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• 2003

해양환경의 대표적 오염물질인 PAHs가 양식굴의 생리대사에 미치는 영향을 파악하고자, 서로 다른 PAHs 농도에 노출된 굴의 여수율, 호흡율, 배설율 및 동화율의 변화를 조사하였다. 부착생물을 깨끗하게 제거한 굴을 실험실로 옮겨 3일정도 순치시키고, 이후 각각의 0, 50, 100, 200ppb의 농도별로 약 10일간 미리 노출시켰다. 이후 각 개체별로 2L 들이 2$0^{\circ}C$항온수조에서 0.2$\mu\textrm{m}$ 여과해수를 사용하여 실험하였다. 여수율은 Chaetoceros simplex를 지표생물로 하여 수조내 시간당 감소율을 구하여 Kim(1995)의 식에 의해 구하였다. 호흡율은 유동파라핀으로 외부와의 공기접촉을 단절시키고, 수조내 단위시간당 DO meter로 산소변화를 측정하여 Widdows and Johnson(1988)의 식을 변형하여 계산하였다. 배설율은 단위시간동안 수조내 NH$_4$-N 농도 증가량으로 조사하였고, NH$_4$-N은 HACH Inc.에서 제공하는 측정법을 따랐다. 여수율은 500ppb에서 다소 영향을 받아 증가하였다. 호흡율과 배설율은 노출농도가 증가함에 따라 증가하였지만, 50ppb와 100ppb에서는 다소 차이가 없었다. 따라서 양식굴이 PAHs와 같은 오염물질에 노출되었을 경우, 초기에는 여수율의 변화는 없고 배설율과 호흡율이 상승하여 체내 에너지 요구량이 증가하는 것으로 생각된다.

• Journal of Animal Science and Technology
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• v.54 no.4
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• pp.291-297
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• 2012

This study was carried out to investigate the effect of dietary phytase on nitrogen and phosphorus excretion of gestating and lactating sows (Yorkshire ${\times}$ Landrace ${\times}$ Duroc). Twelve gestating sows and twelve lactating sows were used in this study and were divided into 2 groups (1 control group and 1 treatment group, 6 replications/group) the control group was without phytase and the treatment group was fed with phytase (750 FTU/kg) in the diet, respectively. Body weights of gestating and lactating sows were $208.9{\pm}13.8$ kg and $190.5{\pm}22.9$ kg, respectively. In gestating sows, feed intake was greater in phytase fed group than the control (P<0.05), but water intake and total excretion were not different between the groups. In lactating sows, feed and water intakes and total excretion were not different between the groups. The N intake of lactating sows was higher in phytase fed group than control (P<0.05) but N excretion ratio was not different. In lactating sows, N intake and excretion and N excretion ratio were not significantly different between the groups. P excretion and excretion ratio in gestating sows decreased by phytase treatment (P<0.05) as compared to control. In lactating sows, N intake and excretion was not significantly different by added phytase (P>0.05). Finally, dietary addition of phytase decreased P excretion in feces of gestating and lactating sows.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.102-102
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• 2003

황복(Takifugus obscurus)은 주로 황해에 분포하며 봄철에 서해안으로 흐르는 금강, 한강 및 임진강으로 소상하여 강의 중상류에서 산란하는 소하성어류이다. 특히 황복은 바다와 하천을 오고 가는 관계로 해수뿐만 아니라 민물에서도 양식이 가능한 어종으로 다른 소하성어류와 마찬가지로 염분변화에 따른 삼투조절능력이 매우 우수할 것으로 생각된다. 본 연구는 황복이 급격히 염분을 달리하였을 때 나타나는 생리적 반응을 산소소비량과 암모니아 질소배설량의 변화로 염분변화에 따른 생리적 적응력을 살펴보고자 하였다. 본 연구에 사용한 황복은 전장 10cm(체중 15~25g) 전후의 치어로서 염분 32, 22, 12, 및 2 ppt로 조절되어 있는 FRP수조(500 L)에서 2개월간 사육 후, 각기 염분을 달리한(32, 22, 12, 2 ppt) 호흡측정 장치에 황복을 수용하여 산소소비량과 암모니아 질소배설량을 측정하였다. 한편, 사육 시스템내에서의 산소소비와 암모니아 질소의 배설경향을 보기 위하여 염분 16ppt에서 산소소비량과 암모니아 질소배설량을 25시간 관찰하였다. 산소소비 경향은 대체로 염분이 낮을수록 높은 산소소비 경향을 보였는데, 실험염분 32ppt에서는 2~32ppt 사육군 모두 다른 실험염분(22, 12, 2 ppt) 사육군보다 안정되고 낮은 산소소비량을 나타내었다. 그리고 실험염분 22, 12 및 2 ppt에서는 각기 실험염분과 동일한 염분 사육군에서 가장 높은 산소소비량을 나타낸 반면 32 ppt 사육군은 모든 실험염분에서 가장 낮은 산소소비량을 나타내었다. 암모니아 질소의 배설경향은 실험염분 2 ppt를 제외하고는 2 ppt 사육군에서 가장 높은 질소 배설율을 보였으나, 실험염분 2 ppt에서는 오히려 현저히 낮은 암모니아 질소 배설율을 나타내었다. 저 염분(2, 12 ppt)에서 사육한 황복은 22 및 32 ppt에서 사육된 황복에 비해 저염분에 노출될 경우 삼투조절을 위해 암모니아 배설에 상당한 차이를 보였다. 사육 시스템내에서 조사된 산소소비와 암모니아 질소 배설경향은 섭이와 관련하여 일간리듬을 보였는데, 산소소비는 섭이 3시간 후에 최고를 보였으며 암모니아 질소 배설은 섭이 4시간 후에 각각 최고를 나타내었다.

• The Korean Journal of Food And Nutrition
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• v.11 no.2
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• pp.237-242
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• 1998

This study was carried out to process canned ark shell with highly quality by management of proper processing conditions for prevention of oxidation and discoloration by heating. Ark shell has hemoglobin as blood pigment in red blood shell which same as other cockles. Hemoglobin is easy to come oxidation and browning reaction, and it has large contents of carotenoid as meat pigment. Proximate compositions in ark shell were 76.9% of moisture, 18.1% of crude protein, 1.8% of crude lipid, 1.3% of carbohydrate and 1.6% of crude ash. And contents of carotenoid and hemoglobin were 0.67~1.02mg% and 0.98~1.64g/dl, respectively. When the living ark shell was soaked in 2% NaCl solutions, about 89% of mud was removed after 10 hours soaking, and over 91% was removed when the pH was adjusted to 7.5. Carotenoid pigment were prepared that extracted from ark shell by using acetone. And determined visible spectrum were two peak at 452nm and 687nm, and λmax were 452nm. During thermal treatment at 95$^{\circ}C$, 111$^{\circ}C$, 116$^{\circ}C$ and 121$^{\circ}C$ for 60 minutes, retention ratio of carotenoid were 71.8%, 66.8%, 64.4% and 36.5%, and after 120 minutes retention ratio were 56.6%, 30.6%, 30.3% and 17.2%, respectively. When heated at 95$^{\circ}C$, 111$^{\circ}C$, 116$^{\circ}C$ and 121$^{\circ}C$, formation of browning material were increased at high temperature and long time treatment.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.1
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• pp.27-35
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• 2005

This study was performed to determine the effect of three different concentrations of soy-isoflavones on excretions through urine and feces in either sham-operated or ovariectomized female rats. Seventy-two 16-week old Sprague-Dawley rats underwent sham operation or bilateral ovariectomy. They were provided diets containing different levels of soy isoflavones for 6 weeks: 50 ppm (low isoflavone intake; LI), 250 ppm (medium isoflavone intake; MI) and 500 ppm (high isoflavone intake; HI). The subsequent fecal and urinary excretions of daidzein and genistein were then measured. In the sham operated rats, body weight gains and food efficiency ratio of the MI and HI groups were significantly lower than control group, while food intake was not different. However, there was no significant difference in ovariectomized rats. The fecal excretion of daidzein was significantly higher in the HI group than the LI and MI groups, and that of genistein increased as dietary isoflavone intakes increased in both the sham operation and ovariectomy. The reverse tendency of fecal recovery was shown with dietary isoflavone dose only in genistein among ovariectomized rats. When dietary isoflavones were increased from 50 ppm to 500 ppm, the amounts of daidzein and genistein in the urine increased dose-dependently. The higher intakes of isoflavones leaded to lower recovery rates of daidzein and genistein in the urine in the sham-operated rats but not in the ovariectomized rats. The urinary recovery was significantly higher in the LI group than the MI and HI groups in the sham-operated rats. The excreted amounts and recovery of the two isoflavones in the urine were higher in the sham operated groups than in the ovariectomized groups, which implied an increased bioavailability of isoflavones by ovariectomy. Therefore, the results suggest that a more efficient use of soy isoflavones in ovariectomized conditions may occur, which indicates that a higher dose of soy isoflavones is necessary for the postmenopausal states.

• Journal of the Korean Society of Food Science and Nutrition
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• v.17 no.2
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• pp.103-109
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• 1988

This research was designed to investigate the influence of dietary fat and cholesterol on the excretions of neutral steroids and bile acids of rats. Experimental groups were divided into 6 groups according to the dietary treatment. A $3{\times}2$ factorial arrangement of treatments included 3 levels of dietary fat(15, 30 or 45% energy) and 2 levels of dietary fat saturation(0.5 or 1.0n P/S ratio). After 4 weeks feeding, fecal excretions of total lipids, neutral steroids and bile acids were determined. The following results were obtained. Body weight gain, feed intake and feed conversion ratio were singnificantly influenced by dietary fat level. Lipid digestibility was significantly increased in proportion to the level of dietary fat. However, no effect has been found in fecal secretions of total lipids, neutral steroids and bile acids by the differences of dietary fat levels and P/S ratios. The consumption of dietary cholesterol improved body weight gain, feed conversion ratio and lipid digestibility, but decreased total lipids excretion. And also, neutral steroids excretion showed tendency of increase with decreasing the fraction of coprostanol and increasing that of coprostanone, and total bile acids showed tendency of decrease by the consumption of dietary cholesterol.

• The Korean Journal of Malacology
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• v.10 no.1
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• pp.55-63
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• 1994

피조개, Scapharca broughtonii의 개체크기(작은 개체: 1.0-1.3cm, 큰 개체:4-5 cm), 수온(10, 15및 2$0^{\circ}C$), 염분(7,13,19,26 및 32 $\textperthousand$)에 따른 일반적인 대사경향과, 수온 1$0^{\circ}C$와 2$0^{\circ}C$ 그리고 부니 농도 25, 50, 100, 200, 400 600, 800및 1000ppm에서 15일간 노출시키면서 사망률, 산소소비율, 여수율 및 배설률을 측정한 결과는 다음과 같다.1. 일반적으로 피조개의개체 크기는 작은 개체일수록, 그리고 수온이 높을수록 비체중 산소소비율, 비체중 여수율 및 비체중 배설률은 높게 나타났다. 그리고 염분을 제외하고는 산소 소비율과 여수율은 염분농도의 증가에 따라 다소 증가하는 유사한 경향을 보인 반면, 비체중 배설률은 염분농도가 낮아질수록 낮은 양상을 나타내었다. 2. 1$0^{\circ}C$의 경우 작은 개체에서 LC$_{50}$은 557.11 ppm, 큰 개체에서 856.03 ppm이었고, 20%C의 경우, 작은 개체에서의 LC$_{50}$은 274.55 ppm, 큰 개체에서 346.26 ppm이었으며 1$0^{\circ}C$에 비해 2$0^{\circ}C$에서 특히 1000 ppm이상의 농도에서 사망률이 현저히 증가함으로써 부니에 의한 영향은 크게 나타났다.3.에 비해 2$0^{\circ}C$의 고수온구에서 부니의 농도가 높을수록 폐사율은 높게 나타났으며, 1$0^{\circ}C$와 2$0^{\circ}C$의 두 수온구에서 산소소비율과 여수율에 대한 각 농도별 부니의 영향은 유사한 경향을 나타내었다. 특히, 2$0^{\circ}C$에서 사망률이 현저히 증가하기 시작하는 부니농도 100ppm이상에서 산소소비율의 감소가 크게 나타났다. 그리고 암모니아태질소 배설률은 부니농도의 증가에 따라 차츰 증가를 보인후 600ppm이후부터는 다시 감소하는 경향을 보였다.

• Journal of Radiation Protection and Research
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• v.32 no.2
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• pp.45-50
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• 2007

In this study, uptake rates of internal organs and daily urinary excretion rates were measured to get more reliable estimation results for Korean. Radioactive iodine($^{131}I$) of $100{\mu}Ci$ was administered by ingestion to 28 adult males for the experiment and then the radioactivity in thyroid gland, liver, stomach, small intestine, kidneys, and urine was measured after time intervals of 2, 4, 6 and 24 hours. Uptake rates of each organ and daily urinary excretion rates were calculated on the basis of these experimental results. As a result, uptake rates of 19.70% for thyroid and daily urinary excretion rates of 71.12%, on the average, were indicated. The maximum of uptake rates and daily urinary excretion rates were recorded after 2 hours of administration of $^{131}I$, but those rates were decreased gradually later. It was also found that uptake rates were the highest in stomach, followed by the left kidney, liver, small intestine and right kidney except for thyroid gland. In this experiment, the calculated uptake change rate in thyroid gland after 24 hours of administration of $^{131}I$ was different from that of ICRP-54/67(30%) and ICRP-78(25%). Thus, it is necessary to apply more reliable approach, reflecting the characteristic of Korean physiology and to obtain the basic data of results using this approach for calculation of the internal adsorbed dose. In the future, this approach can be helpful for the internal dose assessment of radiation workers in a nuclear power plant or in a hospital.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.27 no.4
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• pp.380-389
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• 1994

Larvae of the freshwater shrimp, Macrobrachium nipponense(De Haan) were reared in the laboratory under constant conditions ($25^{\circ}C,\;7\%0$ S), and their feeding rate, oxygen consumption, ammonia nitrogen excretion, and growth were measured at regular intervals during development from hatching to post larval stage. Growth was measured as dry weight, carbon, nitrogen, hydrogen, protein and lipid. All these physiological and biochemical traits revealed significant changes from instar to instar. Average feeding rate was high in intermolt stage of the molt cycle and it showed a bell-shaped pattern. Respiration(R) increased from hatching to post larval stage. Excretion(U) increased in intermolt phase of larvae and it showed a bell-shaped variation pattern, in all larval instars with a maximum near the middle of the molt cycle. Regression equations describing rates of feeding, growth, respiration and ammonia excretion as functions of time during individual larval molt cycles were inserted in a simulation model, in order to analyse time-dependent patterns of variation as well as in bioenergetic efficiencies. Carbon was initially increased and nitrogen showed a tendency to increase in premolt phase during individual molt cycles. Protein remained clearly the predominant biochemical constituent in larval biomass.

• Korean Journal of Poultry Science
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• v.34 no.2
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• pp.119-128
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• 2007

The effects of microbial phytase on laying performance, egg quality, and ileal digestibility of nutrients and amino acids were examined at three levels of phytase (0, 300, 600 unit/kg) in 55-wk-old White Leghorn for 4 weeks. Egg productivity tended to increase with supplemental phytase compared to that of control. Daily feed intake of hens fed phytase also increased. Egg shell thickness was not significantly different among the treatments. Haugh unit and yolk color were not statistically different. However, egg shell breaking strength was high at phytase treatment. Excretion and absorption of nitrogen were no difference among all treatments, but those of phosphorus was higher in the phytase treatment than control. The digestibility was high at crude fiber, crude ash, calcium and phosphorus in nutrients, at lysine, methionine and phenylalanine in essential amino acids, and alanine, cystine, glutamic acid, glycine and tyrosine in non-essential amino acids. In conclusion, supplemental microbial phytase in laying hens diet may help to improve egg production and to decrease P of feces. But, further studies were needed to investigate on the digestibility.