• Proceedings of the Korean Nuclear Society Conference
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• 1998.05b
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• pp.730-734
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• 1998

난포성숙호르몬(FSH)은 난소내 난포의 성장인자로 작용한다. 본 실험은 방사선 조사된 미성숙 생쥐의 난포 폐쇄가 ESH에 의해 억제되는지를 확인하는 한편 방사선어제 개발을 위한 실험모델로서의 가능성을 평가하기 위해 수행되었다. PCNA 에 의한 염색의 정도는 FSH군의 경우, 1이에 최저, 12시간과 8일에 가장 많이 염색되었으며, 방사선을 조사한 후 ESH를 주사한 경우, PCNA에 의한 염색용은 6시간 경과시어 제일 낮게 나타나고 이후 증가하는 경향성을 보였다. 즉, FSH는 방사선에 피폭된 난소내 난포외 apoptosis를 지연시키는 효과를 갖고 있음을 알 수 있었다. 본 연구의 결과, 특정물질의 방사선방어효능 평가를 위한 실험모델로서 난소내 난포를 활용할 수 있으며 특히 FSH가 나타내는 난포폐쇄 억제효과도 방사선방어제 개발을 위한 실험연구에 중요한 역할을 할 것으로 기대된다.

• Journal of Radiation Protection and Research
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• v.29 no.4
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• pp.237-242
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• 2004

The radioprotective effects of Melatonin and Ge-132 on acute hematopoietic injury was investigated in mice exposed to an acute whole-body radiation dose of 8 Gy. Melatonin was administered intraperitoneally 1 hour before irradiation at a dose of 200 mg/kg, and Ge-132 was administered orally from days 5 to 20 after irradiation at a dose 130 - 150 mg/kg/d. The radioprotective effects were evaluated for spleen using TUNEL assay, and in peripheral blood by counting lymphocyte & WBC. The 4 experimental groups (irradiation-only, melatonin pretreatment, Ge-132 posttreatment, and melatonin pretreatment plus Ge-132 posttreatment) were observed for survival analysis up to 30 days following irradiation. The apoptotic index (47.8% vs 45.9%, p=0.385), and the number of lymphocytes ($97/{\mu}{\ell}\;vs\;101/{\mu}{\ell}$, p=0.898) were not significantly different between the irradiation-only and the melatonin pretreatment group, But the number of WBCs ($147/{\mu}{\ell}\;vs\;306/{\mu}{\ell}$, p=0.010) was higher in the melatonin pretreatment group. The irradiation-only, melatonin, Ge-132, and melatonin plus Ge-132 treatments resulted in survival rate at 30 days of 21.4%, 100%, 35.7%, and 85.7%, respectively. The melatonin pretreatment group in survival analysis between groups was showed significantly higher survival than the irradiation-only(p=0.000), or Ge-132 posttreatment group(p=0.0003). These results indicate that the melatonin may have a potential as an effective radioprotector on acute hematopoietic syndrome following radiation exposure.

• Journal of Radiation Protection and Research
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• v.21 no.2
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• pp.99-105
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• 1996

After high-dose irradiation(8 Gy). the viability of lymphocyte with a prior low-dose irradiation was 3.7-fold higher than that without a prior low-dose irradiation The viability could be increased by the reduction of oxygen radicals or the removal of damaged molecules-DNA, protein. lipid membrane. or the removal of damaged cells. In this paper. we studied the radioresistance mechanism in lymphocytes and lymphoma cells by examining the activities of radical scavengers(catalase. peroxidase, superoxide dismutase, and glucose-6-phosphate dehydrogenase), and a radical protector(glutathione). Different enzymes were induced in lymphocyte and lymphoma with low-dose irradiation. The activity of peroxidase increased most(133.3%) in lymphoma while the enzymes increased most in lymphocyte were superoxide dismutase (138.5%), glucose-6-phosphate dehydrogenase (122.4%) and glutathione(120.8%). The activities of these enzymes were highest when the interval was 7 hours between low-dose and high-dose irradiation.