• Title/Summary/Keyword: 방사선돌연변이

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Genotoxicological Safety of the Ethanol Extract from Seafood Cooking Drips by Gamma Irradiation (감마선 조사한 수산 자숙액 에탄올 추출물의 유전독성학적 안전성 평가)

  • Kim, Hyun-Joo;Choi, Jong-il;Lee, Hee-Sub;Kim, Jae-Hun;Byun, Myung-Woo;Chun, Byung-Soo;Ahn, Dong-Hyun;Yook, Hong-Sun;Kim, Keehyuk;Lee, Ju-Woon
    • Journal of Radiation Industry
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    • v.2 no.1
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    • pp.21-26
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    • 2008
  • Although seafood cooking drips were the byproducts from the fishery industry it was known that the cooking drips had many nutrients and could be used as functional materials. Previously, the physiological properties of cooking drips were shown to be increased by a gamma irradiation. But, there was no report on the safe for the genotoxicity on the irradiation. In this study, the genotoxicity of the cooking drips from Hizikia fusiformis, Enteroctopus dofleni and Thunnus thynnus was evaluated by the Ames test (Salmonella typhimurium reversion assay) and the SOS chromotest. The results from all samples were negative in the bacterial reversion assay with S. typhimurium TA98, TA100. No mutagenicity was detected in the assay, both with and without metabolic activation. The SOS chromotest also indicated that the gamma-irradiated seafood cooking drips did not show any mutagenicity. Therefore, this study indicated that gamma irradiation could be used for the hygiene, functional properties and processibility of seafood cooking drips.

Protective Effect of Pesticide on Radiation-Induced Cell Damage in Tradescantia 4430 Stamen Hairs (자주달개비 수술털에서 방사선에 의해 유발되는 세포손상에 대한 살충제의 방어효과)

  • 김진규;김원록;이창주;장화형;이영근
    • Korean Journal of Environmental Biology
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    • v.17 no.1
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    • pp.21-26
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    • 1999
  • To investigate the combined effect of radiation and pesticide on Tradescantia somatic cell mutations, potted plants of Tradescantia 4430 on which parathion had been sprayed evenly 24 hours before irradiation. Radiation doses were 0.3, 0.5, 1.0 and 2.0 Gy of gamma-ray. The plants irradiated only with the gamma-ray radiation were used as control groups (CT). Pink mutation frequency increased linearly proportional to the radiation dose and the peak interval of elevated mutation frequencies appeared during 7 ~ 11 days after irradiation in both CT and Pa +${\gamma}$ groups. The slope of dose -response curve in CT was 5.99 ($r^2$= 0.988), while it was 3.43 (r$x^-2$=0.981) in Pa+${\gamma}$. It seemed that parathion pretreatment had a protective effect against radiation-induced cell damages since it decreased the slope value by 43%. It is suggested that an adaptive response or radiomodification could be induced in irradiated stamen hair cells by parathion pretreatment.

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Effect of Gamma-ray on Survival and Mutation Rates of Rooted Cuttings and Unrooted Cuttings in Rose (감마선 처리에 의한 장미 삽수의 발근 여부에 따른 생존율과 돌연변이 발생빈도)

  • Kim, Se Won;Lee, Hyo Jeong;Kim, Ye-Sol;Jo, Yeong Deuk;Ryu, Jai Hyunk;Kang, Si-Yong;Kim, Sang Hoon
    • Korean Journal of Breeding Science
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    • v.49 no.3
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    • pp.150-156
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    • 2017
  • This study was carried out to compare the survival and mutation rates and mutation spectrum by gamma-irradiation on rooted and unrooted cuttings of three spray type ('Lovelydia', 'Yellowbabe', and 'Haetsal') and two standard type ('Vital' and 'Aqua') cultivars in roses. Two groups, rooted and unrooted cuttings were gamma-irradiated at 70Gy for 24 hours. The irradiated rooted and unrooted cuttings were planted in a greenhouse, and survival, mutation rates and mutation spectrum were investigated 30 weeks after planting, respectively. As a result, survival and mutation rates of gamma-ray irradiated plants were 16.4%~50.8% and 0~5.1% for unrooted cuttings, and 39.4%~55.1% and 0.7%~7.4% for rooted cuttings, respectively. In conclusion, both survival and mutation rates were a little higher on rooted cuttings than on unrooted cuttings. However, when only survived plants after gamma-ray irradiation were considered, mutation rates were 0~10% and 1.8%~14.1% for unrooted cuttings and rooted cuttings, respectively, showing no significant difference. In addition, diverse variations on color and number of petals or shape of flowers were detected both in plants from rooted and unrooted cuttings, which indicated that there was no significant difference in mutation spectrum between two groups.

A New Improved Soybean Variety, 'Josaengseori' by Mutation Breeding (돌연변이 육종에 의한 재래종 서리태 개량 신품종 콩 '조생서리')

  • Song, Hi Sup;Kim, Jin-Baek;Lee, Kyung Jun;Kim, Dong Sub;Kim, Sang Hoon;Lee, Sang Jae;Kang, Si-Yong
    • Korean Journal of Breeding Science
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    • v.42 no.3
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    • pp.222-225
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    • 2010
  • 'Seoritae' is a very famous variety of black soybean for mixed cooking with rice in Korea. However, it has a couple of bad traits such as late flowering and maturity. To improve these characteristics, seeds of original 'Seoritae' were irradiated using a 250 Gy gamma ray in 1994. Some mutants were identified and finally a new soybean variety 'Josaengseori' was developed in 2005. This variety has a few distinguishable characteristics such as smaller grain size, early maturity and high yielding compared to the 'Seoritae'. The flowering period of 'Josaengseori' is 57 days after seeding (DAS), which is 10 days earlier than that of 'Seoritae' (67 DAS). The maturation period of 'Josaengseori' is 130 DAS, which is 34 days earlier than 'Seoritae' (164 DAS). And the total yield of the new variety with 179 kg/10a is 2.4 times higher than that of 'Seoritae' with 74 kg/10a. 100 grain weight of 'Josaengseori' is 32.8 g, which is 20% lower than that of 'Seoritae' with 40.1 g.