• KSBB Journal
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• 제5권4호
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• pp.335-339
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• 1990

In order to develop economic processes for ethanol production from starch, a simultaneous saccharification and fermentation(SSF) process using Zymomonas mobilis and amyloglucosidase (AMG) was studied in semibatch modes using cell recycle. The cell recycle was carried out by adopting two different methods; microfiltration and settling. The cell recycle using microfiltration revealed higher productivity(5.4 g/l/h) than that using a settler(4.3 g/l/h). Taking the large-scale ethanol fermentation into account, the semibatch process using microfiltration system appeared most promising among others with respect to ethanol productivity, feasibility of scale-up and simplification of operation.

• Food Science and Preservation
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• 제9권2호
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• pp.179-183
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• 2002

This study was carried out to set up alcohol fermentation condition for uncooked corn. Response surface methodology(RSM) was applied to optimize and monitor the alcohol fermentation condition with uncooked corn. The optimal yeast strain for fermentation of uncooked corn was Saccharomyces cerevisiae GRJ. The polynomial equation for alcohol contents, brix, pH and total acidity showed 0.8852, 0.9202, 0.8806 and 0.9940 of R$^2$, respectively. The optimal rendition for maximum alcohol contents were 0.18%(w/w) of enzyme concentration and 180%(v/w) of added water content. Predicted values at optimum alcohol fermentation condition agreed with experimental value.

• Journal of Animal Science and Technology
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• 제47권4호
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• pp.513-524
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• 2005

This study was conducted to introduce recycling procedures of food waste(FW) as feed according to the dehydration, semi-dehydration fermentation and liquid fermentation methods through the on-site survey of companies related, to trace physico-chemical components and nutritional losses depending upon the processing stage for each method and finally to suggest more desirable methodology for the efficient utilization of FW as animal feed. For the dehydration method, dewatering of FW alone reduced(P<0.05) moisture(approximately 10%) and ether extract contents and increased(P<0.05) fiber contents. Dewatering and subsequent dehydration of FW decreased(P<0.05) contents of ether extract, limiting amino acids such as lysine, methionine and histidine, pepsin digestibility of protein by half, and NaCl content by 40%, increased(P<0.05) contents of fiber, crude ash, Ca and P, and did not alter(P>0.05) pH. The semi-dehydration fermentation method of FW did not affect(P>0.05) the chemical components, pepsin digestibility of protein, pH and NaCl content. For the liquid fermentation method, pasteurization and fermentation of FW decreased(P<0.05) contents of dry matter, ether extract, crude fiber, lysine and NaCl; however, it did not affect(P>0.05) other chemical components, pepsin digestibility of protein and pH. Among the processing methods, nutrient losses were highest for the dehydration method(25% of metabolizable energy loss, 12% of organic matter loss) and little for the semi-dehydration and liquid fermentation methods. The on-site survey of companies related revealed that the existence of foreign materials in FW products were problematic for all the three companies surveyed, thus it was necessary to develop a more efficient screener. Before feeding FW-containing diets to pigs, high quality of protein and energy feedstuffs needed to be fortified for the dehydration method. For the semi-dehydration fermentation method, the scientific diet formulation technology was required at the initial mixing stage. For the liquid fermentation method, possibly most energetic and proteinaceous feeds needed to be supplemented for the normal animal growth.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 한국식품저장유통학회 2003년도 제23차 추계총회 및 국제학술심포지움
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• pp.202.2-203
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• 2003

전통주의 계승발전을 위해 전통주의 개선과 재현의 필요성이 높아짐에 따라 지난날 맛 좋았던 우리의 전통주의 연구와 개발이 활발히 진행되고 있다. 또한 최근 미곡의 과잉생산으로 인한 과잉쌀 생산에 대한 수요체 창출을 위한 쌀가공품의 개발이 매우 시급한 실정이다. 최근 알콜농도가 낮은 술을 선호하는 경향과 전통주의 특수한 풍미로 인하여 현재 전통주는 많은 사람들로부터 각광을 받고 있어서 현재 몇몇 지역에서 민속주와 토속주가 허가되어 시판되고 있다. 진양주는 전남 해남지역의 전통민속주로서 해남지역에서 생산되는 찹쌀을 원료로 하여 상온에서 발효시키는 독특한 방법으로 제조되는데 이 진양주는 발효과정에서 외부환경의 영향을 많이 받게 된다. 그중 외부온도는 진양주의 품질에 직접적인 영향을 미치게 되는데 아직까지는 진양주제조과정에서 최적발효온도에 대한 연구는 보고된 것이 없다. 본 연구에서는 발효온도를 2$0^{\circ}C$와 $25^{\circ}C$로 하였을 때 발효과정에서 술의 품온, pH, 당도, 알콜함량, 총산의 변화를 조사하였으며 관능검사를 통하여 종합적인 기호도를 평가하여 최적의 발효온도를 탐색하고자 했다. 발효과정에서 술의 품온은 온도계를 사용하여 측정하였고 pH는 pH-meter로 측정하였으며 당도는 당도계를 이용하여 측정하였다. 알콜함량과 총산은 국세청의 주류분석규정에 따라 증류법과 중화적정법을 사용하여 측정하였다. 관능검사는 식품공학과 대학원생과 학부생 10명을 대상으로 종합적인 기호도에 대하여 5단계 평점법으로 실시하였다. 2$0^{\circ}C$에서 발효시 품온은 20.2~23.8$^{\circ}C$사이에서, $25^{\circ}C$에서 발효시 품온은 25.2$^{\circ}C$~28.2$^{\circ}C$사이에서 변화되었고 pH는 2$0^{\circ}C$ 발효시 3.47~4.62 사이에서, $25^{\circ}C$ 발효시 pH는 3.28~4.65사이에서 변화되었고 최종 pH는 2$0^{\circ}C$에서 발효시 3.71로써 $25^{\circ}C$ 발효시 3.58보다 높았다. 최종당도는 2$0^{\circ}C$, $25^{\circ}C$ 발효시 각각 10.2。Brix와 8.4。Brix 였고 알콜농도는 2$0^{\circ}C$발효시 13.8%로 $25^{\circ}C$ 발효시 14.8% 낮았으며 총산은 2$0^{\circ}C$ 발효시 1.32%로 $25^{\circ}C$ 발효시 1.62%보다 낮게 나타났다. 관능검사를 통한 종합적인 기호도는 2$0^{\circ}C$에서 발효시킨 진양주가 4.2로써 $25^{\circ}C$에 발효시킨 진양주 3.5보다 높은 것으로 나타났다. 관능검사시 $25^{\circ}C$에서 발효한 진양주는 신맛이 많이 난다는 결과로 볼 때 최종 pH가 3.58로 낮고 총산이 1.62%로 높아서 나온 결과라고 생각된다. 결론적으로 진양주를 제조할 시 발효온도는 2$0^{\circ}C$가 발효온도 $25^{\circ}C$보다 더 적합하다고 생각된다.

• Korean Journal of Food Science and Technology
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• 제38권4호
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• pp.475-482
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• 2006

The quality characteristics and functional properties of fermented soybean by lactic acid bacteria (FSB) were investigated and compared with those of soybean (control) and commercial cheonggukjang Powder (CGP). The crude protein, lipid, and fiber contents of FSB were similar to those in CGP. The vitamin $B_2$ content in FSB (1.4 mg%) was similar to the control (1.3 mg%) whereas it was remarkably low in the CGP (0.2 mg%). The bright yellow color of FSB determined by Hunter's colormeter was quite different from the dark reddish brown color of the CGP. The pH of FSB was the lowest and thee amino-nitrogen was 517.2 mg%, which was higher than that in CGP (468.1 mg%). Glutamic acid, aspartic acid and leucine were the most abundant amino acids. In particular, the increase in the glutamic acid level was noticeable in FSB. The fatty acid compositions of FSB and CGP were similar to the control. However, the sucrose and fructose levels were lower after fermentation but the glucose level was higher. The results of isoflavone analysis by HPLC showed that the levels of daidzein and genistein in FSB were as much as 48.33 and 52.82 mg%, respectively, which is higher than that found in CGP. The DPPH free radical scavenging effects of FSB and CGP were 1.8 times higher an those of the control. The fibrinolytic activity determined by the diameter of the lysed area on the fibrin plate was the most effective in FSB. In conclusion, it is believed that FSB has a similar or higher quality than CGP. Therefore, FSB is expected to be good functional food material.

• Journal of the Korea Organic Resources Recycling Association
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• 제6권2호
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• pp.95-112
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• 1998

For the effective disposal of organic food wastes, we seleted 4 strains of microorganism from 186 microbial candidate via enzyme activity test, salt tolerance, food decomposition rate, stability and safety of strains. The identity of these 4 strains are as follows : Fungi is Rhizopus sp., yeasts are Galactomyces sp., Pichia sp. and Hyphopichia sp., In the 50L fermenter scale, we tested various fermenting factor for the optimization of conditions of food waste decomposition using 4 selected strains. The optimum fomenting conditions were as follows : BIO-CHIP Volume 25-30 L, BIO CHIP size 2.0-6.0mm, air flow 200-280L/min, mixing intensity 2-4rpm, temperature $30-45^{\circ}C$. In these fermenting conditions, the efficiency of decomposition(rate of weight loss of food wastes) were 93%. Also the quality of fermenting output were assayed at the basis of fertilizer, and the results were as good as general compost.

• Journal of the Korean Society of Food Science and Nutrition
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• 제30권4호
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• pp.617-622
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• 2001

Changes of quality properties of fermented tofu prepared with two molds like Actinomucor elegans (AE) and Rhizopus oligosporus (RO) and coagulants (CaCl$_2$ and citric acid) were investigated. Moisture and crude protein of fermented tofu were rapidly decreased during fermentation, the contents of crude lipid and crude ash were shown to be slightly increased, ad then total acidity was slowly decreased. The content of reducing sugar of fermented tofu was slowly increased for 7 day of fermentation, but rapidly increased after that time because of rapid hydrolysis of carbohydrate in fermented tofu. The contents of amino and ammonia type nitrogen were quickly increased during fermentation. The highest contents of amino type nitrogen of fermented tofu were found in sample of CaCl$_2$group as a coagulant and RO group as a mold. Contents of minerals in tofu fermented for 14 day were high in order of K>Ca>Mg>Na. Iin conclusion, AE was more effective than RO to enhance the contents of reducing sugar and amino type nitrogen as an indicator of fermentation within 7 day of fermentation, and then RO was more effective than AE after that time. Calcium chloride as a coagulant was more effective than citric acid in tofu fermented with the same strain for 14 day.

• Proceedings of the Membrane Society of Korea Conference
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• 한국막학회 1992년도 춘계 총회 및 학술발표회
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• pp.5-6
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• 1992

폐수처리장치등의 발효조와 매립지등에서 발생하는 biogas는 CO$_2$와 CH$_4$를 주성분으로 하여 $H_2S$, 할로겐화합물, 방향족화합물 등을 포함하고 있으므로 이들 성분들로 인한 환경오염의 방지와 함께, $CH_4$를 가스터빈에 의하여 전력을 생산하거나 천연가스에 상당하는 경제성있는 에너지원으로 변환시키기 위하여 분리, 정제 공정이 필요하다. 본 연구에서는 biogas의 주성분을 이루는 CO$_2$와 CH$_4$를 상용 Cellulose Acetate 막을 이용하여 분리함에 있어서 분리효과에 대한 두 기체와 막 사이의 상호영향을 알아보고자 하였다.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 한국미생물생명공학회 1976년도 제8회 학술발표회
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• pp.186.3-187
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• 1976

섬유소단세포단백의 공업적 생산을 위하여 실험적인 결과를 토대로하여 Jar Fermentor에 의한 scale up 실험을 하였고, pilot plant scale의 발효조를 이용하여 대량생산 실험을 하였는 바 그 결과를 발표하고자 한다. 1. 배양중 생성되는 유기산을 중화하기 위하여 주입되는 암모니아의 소비량과 균체농도는 배양종반을 제외하고는 거의 비례하였으며 암모니아소비량으로 균체생육 정도를 간접적으로 판단할 수 있었다.(중략)

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 한국어업기술학회 2001년도 추계 수산관련학회 공동학술대회발표요지집
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• pp.277-278
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• 2001

최근 경제적이고 안정적인 rotifer 배양방법으로 담수산 농축 Chlorella를 이용한 rotifer 고밀도 배양이 수행되었고 이러한 배양방법은 rotifer의 양적 확보에 매우 효과적인 것으로 나타났다. 이처럼 고밀도 배양에 이용되는 담수산 농축 Chlorella는 무균 발효조에서 배양되기 때문에 가격이 매우 싸고, 세포내 rotifer 성장에 많은 영향을 미치는 B$_{12}$함량이 매우 풍부하다. (중략)