• Title/Summary/Keyword: 발효방법

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Quality Improvement of Wines Made from Domestic Grapes by the Elimination or Addition of Grape Skins (포도껍질의 제거 또는 첨가를 통한 국내산 포도주의 품질개선)

  • Yook, Cheol;Jang, Eun-Mi
    • Korean Journal of Food Science and Technology
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    • v.41 no.5
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    • pp.528-535
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    • 2009
  • Three domestic varieties of red grapes were utilized for the fermentation of wines, including Campbell, Muscat Bailey A (MBA), and Sheridan. The grapes were treated by four different methods before fermentation, which included conventional fermentation for red wine, fermentation with skin-removed grapes, fermentation with grapes and addition of removed skins, and fermentation with grape juice. Three different grape varieties with four different treatments did not show any differences in terms of $^{\circ}Bx$ and alcohol changes during fermentation. However, the L value of the MBA wine was lower and darker than the L values of wines prepared from Campbell and Sheridan grapes. The wines prepared from grapes with the addition of removed skins were darker and had 30% higher polyphenol concentrations than those of wines made from grapes by the conventional method, regardless of the grape varieties. Sensory evaluations of dry-type wines demonstrated that quality was higher for those made from Campbell and MBA grapes with the addition of removed skins than for wines made by the conventional method. Whereas for sweet-type wines, the wine that was made from the skin-removed grapes (Sheridan) was more favorable than that made by the conventional method.

Production of Bio-ethanol from Brown algae by Enzymic Hydrolysis (효소적 가수분해에 의한 갈조류 바이오 에탄올 생산)

  • Lee, Sung-Mok;Choi, In-Soon;Kim, Sung-Koo;Lee, Jae-Hwa
    • KSBB Journal
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    • v.24 no.5
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    • pp.483-488
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    • 2009
  • The Brown-algae polysaccharide consisting of alginate and laminaran is usable as high bio-ethanol production if hydrolyzed to monomer unit. The objective of this study is to produce bio-ethanol from brown-algae using enzymatic saccharification. Bio-ethanol was produced by Saccharomyces cerevisiae KCCM 1129 and Pachysolen tannophilus KCTC 7937 strains. The substrate used Laminaria japonica, Sargassum fulvellum and Hizikia fusiformis. We isolated a new alginate lyase and laminaran lyase producing microorganism for hydrolysis of brown-algae from southern sea of Gijang. The reducing sugar was obtained 1.90 g/L from Laminarin japonica 20 g/L that used enzyme from Bacterium antarctica. In pretreatment of the most suitable brown-algae for ethanol production, ethanol concentration of 0.93 g/L and yield of 4.65% were obtained in condition of Laminaria japonica in medium.

Quality Characteristics of Fermentation Gastrodia elata Blume by Saccharifying Methods (당화방법에 따른 유산균 발효천마의 품질특성)

  • Song, Young-Eun;Choi, So-Ra;Song, Eun-Ju;Seo, Sang-Young;Lee, In-Sok;Han, Hyun-Ah;Lee, Ki-Kwon;Song, Young-Ju;Kim, Young-Hoi;Kim, Myung-Kon;Park, Shin-Young
    • The Korean Journal of Food And Nutrition
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    • v.29 no.5
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    • pp.698-705
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    • 2016
  • This study aimed to evaluate the quality characteristics of Gastrodia elata Blume fermented by lactic acid bacteria after saccharifying by 3 methods including enzyme, malt, and rice-nuruk. The lactic acid bacteria (LAB), Pediococcus inopinatus BK-3, isolated from kimchi could reduce the unpleasant taste and odor of Gastrodia elata Blume. The total acidity value of Gastrodia elata fermented by LAB on the malt and rice-nuruk extract solution for 3 days was 2.23% and 2.33%, respectively. After saccharification by malt and rice-nuruk extract solution for 3 days, the viable cell number of fermented Gastrodia elata was 9.14 log cfu/mL and 9.27 log cfu/mL, respectively. The total acidity values were increased above 3.35% by malt and rice-nuruk extract solution for 8 days. Thus, the viable cell number was the highest by malt and rice-nuruk extract solution fermentation for 3 days. The amino acid content of Gastrodia elata fermented by LAB after saccharification by malt extract solution was higher than that of other saccharifying methods. The free sugar content and p-hydroxybenzyl derivatives induced by the enzyme method were higher than those of other saccharifying methods. The overall acceptability was the highest at 4.2 point in Gastrodia elata fermented by malt extract solution.

An Effect of Visual Acuity Protection by Natural Pigment (Anthocyanine) Extracted from Fermented Purple Sweet Potato (발효 자색고구마 추출 천연색소(anthocyanine)에 의한 시력보호 효과)

  • Seo, Eun-Sun;Ryu, Geun-Chang
    • Journal of Korean Ophthalmic Optics Society
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    • v.17 no.4
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    • pp.395-401
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    • 2012
  • Purpose: The purpose of this study was to research any effect on vision protecting or decreasing VDT syndrome of extracted anthocyanine from fermented purple sweet potato and blueberry. Methods: Subjects were aged 19-20 years old who do not have ophthalmic and systemic diseases and over -N4.00 D of refraction error. 40 mg of extracted anthocyanine from fermented purple sweet potato, from blueberry, and control group, placebo were dosed at separate try. After 2 hours later, subjects were directed perform visual display terminal (VDT) work for 2 hours. Objective refractive error was measured before dosing anthocyanine and after VDT work for 2 hours. Degree of head ache, eye pain and strain and subjective symptoms of neck, shoulder and waist was also examined through interviews by dividing its degree into severe, moderate, slight or none. Results: After 2 hours VDT work, vision protection effect in terms of refractive error for dominant eye was decreased by $0.031{\pm}0.21$ D in the group of extracted anthocyanine from fermented purple sweet potato, $0.006{\pm}0.32$ D in the group of extracted anthocyanine from blueberry. However, there was significantly myopic progression in the placebo group by $0.144{\pm}0.28$ D (t=2.27, p=0.03). Conclusions: It is considered that extracted anthocyanine from fermented purple sweet potato inhibits increase of refraction anomalies of dominant eye rather than non-dominant eye after VDT work.

Effects of Pre-treated Sub-ingredients and Deodorization Materials on the Kimchi Smell during Fermentation (전처리된 김치 부재료와 냄새 흡수 물질이 발효중 김치냄새에 미치는 영향)

  • Ku, Kyung-Hyung;Kim, Young-Jin;Koo, Young-Jo;Choi, In-Uook
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1549-1556
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    • 1999
  • This study was conducted to investigate the effects of pre-treated sub-ingredients and deodorization materials on the smell intensity of Kimchi during fermentation.Among the various sub-ingredients of Kimchi, garlic, ginger and green onion have comparatively strong smell. The smell intensities of the sub-ingredients, which were pre-treated with various patented methods were examined using the sensory evaluation method and AromaScan. The results showed that the good methods to reduce the smell of sub-ingredients were hot air drying and soaking with heating treatment. The pH, titratable acidity and the number of microorganism of Kimchi prepared with pre-treated sub-ingredients were not different among samples during fermentation. The smell intensity of the Kimchi with pre-treated sub-ingredients was weaker than that of control until ten fermentation days, but the smell intensity after 10 days of fermentation was not different among samples. The addition of ${\alpha}-cyclodextrin\;and\;{\beta}-cyclodextrin$, which are known to have deodorization effect, at a level of 0.1% respectively, to Kimchi resulted in no difference in the pH, titratable acidity and smell intensity during fermentation at $10^{\circ}C$ compared to those of control Kimchi. However, the addition of deodorizer reduced sulfide classes such as methyl allyl sulfide, dimethyl disulfide, allyl sulfide, methyl propyl disulfide up to 50%.

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Simultaneous Determination of Carbohydrates and Organic Acids in Various Cultured Dairy Foods by High-Performance Liquid Chromatography: A Preliminary Study (다양한 낙농 발효유제품에서 HPLC를 이용하여 탄수화물과 유기산의 동시 검출에 관한 연구)

  • Kim, Dong-Hyeon;Hwang, Dae-Geun;Chon, Jung-Whan;Kim, Hyunsook;Kim, Hong-Seok;Song, Kwang-Young;Yim, Jin-Hyuk;Kim, Young-Ji;Kang, Il-Byung;Lee, Soo-Kyung;Seo, Kun-Ho
    • Journal of Dairy Science and Biotechnology
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    • v.33 no.4
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    • pp.263-269
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    • 2015
  • Various carbohydrates (lactose, glucose, and fructose), lactic acid, uric acid, and acetoin were separated on a ZORBAX Carbohydrate Analysis column using the Agilent 1200 HPLC ChemStation$^{TM}$, and were identified according to retention times with 325 Dual Wavelength UV-Vis Detector and Refractive Index Detector with 0.013 N $H_2SO_4$ at a flow rate of 0.8 mL/min. In addition, the lactase activity of four commercial probiotic lactic acid bacteria during 6-hour incubation was determined using a high-performance liquid chromatography (HPLC) method. Among the tested samples, Bifidobacterium animalis subsp. lactis showed the greatest lactase activity, followed by Lactobacillus rhamnosus and Lactobacillus casei, with Streptococcus salivarius subsp. thermophilus showing the lowest activity. Therefore, this HPLC technique shows potential for evaluating the fermentation processes of probiotic lactic acid bacteria and could simultaneously confirm the degree of ripening in various fermented dairy foods within only a half hour.

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The experiment of process efficiency and salt elimination in food waste compost using triple salt (삼중염을 이용한 음식물 쓰레기 퇴비 중 염분제거 및 공정효율화 실험)

  • Kim, Nam-Cheon;Jang, Byung-Man
    • Journal of the Korea Organic Resources Recycling Association
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    • v.14 no.2
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    • pp.83-90
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    • 2006
  • The NaCl contents of food waste composts made by various techniques known up to now were under the level of 1% by fresh weight basis. But these techniques has some problem that is environment pollution from treated water and high equipment cost. The application to agricultural land of food waste compost that is not sufficiently removed NaCl was considered to be improper due to salt accumulation in soils and plant growth inhibition by salt stress. The purpose of this study is to decompose NaCl in food waste compost using triple salt and this method is differ from existing chemical method. Also, reaction of NaCl with triple salt produced KCl that is basic material of potassium fertilizer. Moreover Also, there was temperature rise of average $5^{\circ}C$ as result that apply triple salt in food waste 600 ton in food wast composting productive capacity. Obvious odious smell reduction effect appeared pretreatment process and fermentation process with temperature rise and this is because triple salt activation of aerobe and removes odious smell cause material by salt content decrease effectively.

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The Situation of Mushroom Cultivation Growing at High Temperature in Africa Malawi (아프리카 말라위의 고온성 버섯재배 현황)

  • Chang, Hyun-You;Kim, Seon;Baek, Soon-Jib
    • Journal of Practical Agriculture & Fisheries Research
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    • v.14 no.1
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    • pp.195-209
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    • 2012
  • 1) 버섯종균 생산 시스템 개선 버섯의 품질과 수량을 좌우하는 주요 요인은 종균의 활력이다. 그러나 말라위에서는 종균의 활력이 낮아 조그마한 350cc 음료수 병에 배양 완성하는데 20일이 소요되는 등 노동력의 비효율성을 개선하였다. 따라서 350cc 병을 3500cc 비닐봉지로 개선하여 입봉하는데 소요되는 시간을 10배 이상으로 단축 개선하였다. 또한 곡립종균을 액체종균으로 대처한 결과 균사생장 속도가 빨랐으며 작업속도가 빨라졌다. 곡립 수수를 밀로 대체하여 종균제조 노동력을 효율적으로 전환하였다. 2) 버섯재배사 환경관리 개선 버섯재배의 4대 환경조건은 온도, 습도, 환기, 광이다. 그러나 말라위의 버섯재배사는 4대 환경조건 조절을 자연상태에 의존하고 있다. 그럼에도 불구하고 비닐로 커버된 상태에서 문을 닫아두어 과습이 되고 환기가 되지않았다. 이를 개선하기 위하여 직경 5cm 환기공을 버섯재배사 당 4개씩 만들도록 지도하였다. 3) 버섯배지 개선 말라위에서는 옥수수 대를 잘라 버섯배지로 주로 이용하고 있다. 이를 개선하여 말라위 북부지역(다광)에 대규모 사탕수수 공장을 방문하여 사탕수수 박을 이용하여 버섯재배 기술을 교육하였다. 이를 이용함으로서 버섯 균사생장속도를 빠르게 하고 균사량의 축적을 높이도록 하여 고품질 다수확하는데 교육을 하고 컨설팅을 실시하여 개선하였다. 4) 버섯 배지 야외발효 후 지면재배 시도로 시간 단축과 비용절감 말라위 버섯배지 혼합제조법은 옥수수대 한가지로서 영양원이 없다. 이를 개선하여 배지를 수분을 흡수시킨 후 야외 퇴적하므로서 배지의 물리성을 개선하고 야외발효 과정 중 양분이 합성되어 양질의 배지를 만드는 방법이다. 야외발효한 배지를 봉지에 담아 균상에 입상하는 불편함과 나무로 만들어진 균상대의 과습으로 인한 곰팡이 발생과 개미 침입으로 썩는 것 등을 방지하기 위하여 지면재배 방법으로 개선하였다. 이 지면재배는 균상 만드는 나무재료와 인건비 절감. 병해충 발생 방제 뿐만아니라 고품질 다수확할 수 있는 방법으로 개선 교육과 시범 시연을 실시하였다.

Citrus Wine-making from Mandarin Orange Produced in Cheju Island (제주도산(濟州道産) 감귤발효주(柑橘醱酵酒)의 양조특성(釀造特性))

  • Koh, Jeong-Sam;Koh, Nam-Kwon;Kang, Soon-Sun
    • Applied Biological Chemistry
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    • v.32 no.4
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    • pp.416-423
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    • 1989
  • In order to produce clear and favorable citrus wine from Citrus unshiu produced in Cheju island, chemical and microbiological processes for alcoholic fermentation were investigated. The ratio of pressed juice passed below 100 mesh sieve and peel of mandarin orange were 55.9% and 25.6% respectively. Orange juice for fermentation source contained 8.85% total sugar, 1.43% total acid and 0.056% volatile acid. Pressed juice was adjusted to 24 degree Brix with cane sugar, and was fermented at $20^{\circ}C$ for one month. Starter screened and selected was Saccharomyces cerevisiae IAM 4274. As principal fermentation proceeded for one week, suspended solids began to precipitate slowly after then. After fermentation, clear citrus wine consisted of about 8 degree Brix residual sugar, $13.3{\sim}14.4%$ ethanol, $0.78{\sim}1.11%$ total acid, $0.05{\sim}0.07%$ methanol and $2.25{\sim}3.29%$ extract, was obtained. Color, flavor and taste of citrus wine found good with panel test. Citrus wine which was treated with fungal enzyme derived from Aspergillus niger CCM-4 was cleared much faster, and could be filtered more rapidly than the untreated. The enzyme-producing strain was isolated from field soil of Cheju island and identified.

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