• Journal of Korean Society of Forest Science
• /
• v.85 no.2
• /
• pp.300-308
• /
• 1996

This study was conducted to identify factors involved in rooting of Japanese larch cuttings. Cuttings were taken every week from June 24th to July 25th before and after the 10th of July which supposed to be best time for cutting. The content of IAA, carbohydrate, nitrogen and inorganic elements such as $Mg^{{+}{+}}$, $K^+$ and $Ca^{{+}{+}}$ within cutting materials were analyzed. After the cuttings were planted, the root development was observed every week. Cuttings of easy-to-root clones were higher in IAA contents than the cuttings of hard-to-root clones at the time of cuttings were taken. There were significant differences in moisture content of cuttings taken on June 24th and July 25th between easy-to-root and hard-to-root clones, however no significant differences in moisture contents were observed between them taken on the 8th and 15th of July which supposed to be the best time for cutting. Inorganic matters and carbohydrate contents of cuttings taken from hedged trees were higher than those of non-hedged trees. The ratios K/C and C/N of cuttings taken from hedged trees were higher than those of non-hedged trees. Cuttings taken from easy-to-root clones showed higher content of carbohydrate and nitrogen than those of hard-to-root clones. The contents of inorganic elements, such as $Mg^{{+}{+}}$, $K^+$, and $Ca^{{+}{+}}$ in cutting taken from hard-to-root clones were slightly higher than that of easy-to-root clones. The ratio C/N and K/C of cuttings which were taken from easy-to-root clones have shown a similar trend of carbohydrate and nitrogen. These data indicate that there was no one dominant factor that affect rooting of cutting, but many factors such as contents of IAA, carbohydrate, nitrogen, phosphate, and ratios of C/N and K/C appeared to affect on rooting of cuttings variously. Most of root initials were formed from phloem tissue within one week after cutting, and few were formed from callus tissue. Root primordia were developed from root initials within two weeks after cutting, and most of new roots were developed from root primordia within about five weeks after cutting.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.11 no.1
• /
• pp.95-101
• /
• 2009

This study was carried out to investigate the optimum condition of germination temperature and cutting condition in Thymus quinquecstaus. A germination rate was superior at light condition and 20℃ in temperature(rooting rates : 89%) Rooting rates by cutting times were the highest in 20th May(rooting rates : 94%) The best Cutting media for rooting rates and member of roots observed peatmoss (50%)+perlite(50%) among the Cutting media. The rooting rates by concentration of growth hormone were the highest(96%) at IBA 100·mg/l among the treatment.

• Journal of Plant Biotechnology
• /
• v.41 no.2
• /
• pp.94-99
• /
• 2014

This study was conducted to elucidate the effect of light sources and explant types on in vitro shoot multiplication and rooting of a rare and endangered plant Abeliophyllum distichum. Both apical buds and axillary buds were used as explants under 4 different light sources, cool white florescent light (F), 100% blue light-emitting diode (LED) (B), 50% blue and 50% red LED mixture (BR), and 100% red LED (R). Clear difference was observed in terms of shoot proliferation by light sources types but not by position-dependent explant types. Multiple shoot induction rates were enhanced under both B and BR light sources. Spontaneous rooting was induced in shoot induction medium under B light source. Both the rates of rooting and numbers of roots per explant were higher in apical bud explants compared to axillary bud explants. Interestingly R light source stimulated shoot elongation but inhibited root development. Therefore, our results suggest that the use of apical bud explants under B or BR light sources is suitable for in vitro micropropagation of a rare and endangered plant species, Abeliophyllum distichum.

• Horticultural Science & Technology
• /
• v.30 no.1
• /
• pp.13-20
• /
• 2012

This study was carried out to examine the effect of cutting condition on the growth of rooted cuttings and their subsequent growth of cut flower in plug cutting of Dendranthema grandiflorum 'Baekma'. The more leaves were attached to the cutting, the higher shoot growth of rooted cutting was observed. Cutting with two to six leaves was effective in shoot growth and rooting than any other treatment. Shoot growth of rooted cutting was not affected by cutting length, but rooting was better in 5 to 7 cm long cutting. Shoot growth and rooting of rooted cutting was promoted by increasing the cutting diameter, and rooting was better in 3.6-4.2 mm thick cutting than 3.1 mm thick cutting. As the treating concentration of NAA increased, shoot growth was inhibited and root length shortened in rooted cutting. Soaking with $100mg{\cdot}L^{-1}$ ${\alpha}$-naphtalene acetic acid (NAA) for 1 h was effective in shoot growth and rooting. Root growth such as root number, root length, and root weight was better in rooting medium mixed with 2:2 of peatmoss:perlite than the other treatments. When rooted cutting produced from cutting with four or six leaves was planted, better length, weight, and leaf number of cut flower was observed. In rooted cutting produced with 7-9 cm long cutting, growth of cut flower such as length, weight, and ray flower number was more effective than the others. Therefore, it is recommended that the 7 cm long and 3.6 mm thick cutting with four to six leaves is used to improve their rooting and subsequent growth of cut flower in plug cutting of Dendranthema grandiflorum 'Baekma'.

• Journal of Plant Biotechnology
• /
• v.35 no.3
• /
• pp.229-234
• /
• 2008

An efficient micropropagation was established by using axillary bud explants from two-year-old tree(Echinosphorea koreensis Nakai), which has been known as a rare and endangered species. Among various basal media tested, DKW medium was shown to be the best for axillary shoot elongation. The addition of both BA and TDZ to the medium induced 6 to 10 shoots per explant during eight weeks of culture, without showing any abnormal morphology at the shoot proliferation stage. However, high concentration of TDZ(>0.05 mg/L) appeared to cause hyperhydration on either leaf or shoot at the later developmental stage. Approximately 20% of shoots produced roots by the addition of 1.0 mg/L NAA but not by IBA($0.2{\sim}1.0$ mg/L). Ex vitro micro-cuttings were better source for root induction; up to 58.6% of the micro-cuttings rooted when 100 mg/L IBA was applied to the soil(vermiculite). More than 90% of plantlets with roots were successfully acclimatized and grew normally in the field. Therefore, we suggest that this endangered tree species can be effectively micropropagated by axillary bud culture system developed in this study.

• Journal of Plant Biotechnology
• /
• v.43 no.4
• /
• pp.486-491
• /
• 2016

The aim of this research is to establish shoot regeneration system for 'Wonhwang' pear (Pyrus pyrifolia L.) using various concentrations of 1-Naphthaleneacetic acid (NAA) 0.01, 0.05, 0.1, 0.5 mg/L in combination with benzylaminopurine (BA) 3, 5, 10 mg/L. Medium containing 4.4 g/L of Murashige and Skoog (MS) medium with vitamins containing 8 g/L of plant agar and 30 g/L of sucrose with NAA 0.05 mg/L and BA 3 mg/L showed 13.3% of shoot regeneration rate. 'Wonhwang' showed no root growth on existing rooting media of P. pyrifolia cv. Niitaka, 'Whang-keumbae' and 'Bae Yun No. 3'. We evaluated the effect of concentration and kinds of plant growth regulators and carbon source to establish efficient rooting condition for 'Wonhwang' pear. In the result of using various concentrations of NAA 0.5 mg/L and 1.0 mg/L in combination with indolebutyric acid (IBA) 3, 5, 10 mg/L, rooting rate of 24% was observed using 1/4 Linsmaier and Skoog (LS) medium supplemented with 7.5 g/L glucose as carbon source and IBA 1.0 mg/L with NAA 1.0 mg/L.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.39 no.2
• /
• pp.115-120
• /
• 1994

To find out the effects of preharvest sprouted degrees of barley on yield, grain quality and germination rate, various sprouted grains were planted. The grains sprouted upto 2 mm and 4mm of root lengths showed regerminating rates of 68% and 49% respectively, and those above 4mm of root length decreased seriously in regerminating rate. First of all, the PI (promptness index) of sprouted barley compared with that of intact barley decreased conspicuously even in 2mm of root length. Grain yield decreased inversely with the growth of root lengths of sprouted grains. The times required to polish the naked barley up to polishing yield of 72% and husked one upto that of 64% were shortened inversely with the root lengths of sprouted grains. The rate of split kernel against sound one after polishing increased greatly by 17.6% to 36% in sprouted barley compared to 2% to 3.5% of the control which presoaked in water for 2 hours. Whiteness indexes of polished kernels of the sprouted barley and the control were higher than that of intact one. The index, however, was lowered inversely with root lengths in sprouted barley.

• Korean Journal of Medicinal Crop Science
• /
• v.3 no.3
• /
• pp.246-250
• /
• 1995

Scions from the one- and two-years old and the nodes of Ligusticum chuanxiong stem were used to study the rooting ability with the treatment of plant growth regulators planted to different bed soils as the alternative propagation for the present method by rhizome. The rooting ratio in the scion from the one- and the two-years old plants showed any difference, but that was ranged from 94% to 100% in the first node and from 56% to 64% in the second node from the bottom of the stem. The scion of first node from both the plants showed more than 90% in the root ratio and 16 in the number of rooting and growed more than 31cm in the root length. The Rootone-F and 250ppm of NAA were most effective for the promotion of rooting. Rooting ratios in the sand and in the mixture of vermiculite and perlite as bed soils were about 18%, but that in the cultured soil considerably decreased.

• Journal of Bio-Environment Control
• /
• v.23 no.4
• /
• pp.288-292
• /
• 2014

This study was performed to investigate the possibility of water cutting by vegetative propagation method from July to August of Clematis. To find out proper cutting length and rooting growth regulator for water cuttings production, cutting lengths of C. 'Honora' made 1 node and 2 nodes length cutting, which were soaked in NAA $0.1mg{\cdot}L^{-1}$. Rooting rate of cuttings treated by NAA $0.1mg{\cdot}L^{-1}$ was 100% irrespective of root lengths, but that of 1 node and 2 nodes untreated by NAA $0.1mg{\cdot}L^{-1}$ were very low about 30% and 19% respectively. Number of roots and roots length were also highest in NAA $0.1mg{\cdot}L^{-1}$, effects of NAA $0.1mg{\cdot}L^{-1}$ treatment were greater at 1 node cutting. Based on the results, rooting rate for I node cutting of C. 'Pairu' according to NAA (0, 0.1, 0.5, $1.0mg{\cdot}L^{-1}$) concentrations was investigated. Days to rooting were 70-74 days (about 10 weeks), which was not significantly different among the NAA concentrations. Rooting rate increased by NAA concentration. it was about 90% usually and was improved with more than NAA $0.5mg{\cdot}L^{-1}$.

• Korean Journal of Plant Tissue Culture
• /
• v.27 no.1
• /
• pp.63-69
• /
• 2000

In order to establish a micropropagation system for buffalo gourd (Cucurbita foetidissima ) and common milkweed (Asclepias syriaca), the effects of several plant growth regulators and culture temperature on shoot multiplication and rooting were investigated. In buffalo gourd, the greatest number of shoot from shoot tip culture and well growth of formed shoot were obtained on the MIS medium supplemented with 1.0 mg/L BA and 0.3 or 0.6 mg/L IAA. Whereas kinetin and 2iP were not effective for shoot multiplication in vitro. It was found that 22$^{\circ}C$ and $25^{\circ}C$ were suitable for shoot multiplication. Roots were easily formed by the addition of auxins, especially 1.0 or 2.0 mg/L IBA and 2.0 mg/L IAA. Over 90% of plants survived successfully after being transferred into the field. In common milkweed, BA was more effective than kinetin or 2iP for its micropropagation in vitro. The increased shoot weight and number of nodes per shoot were obtained on the medium containing 3.0 mg/L BA and 0.3 or 0.6 mg/L IAA. But 2iP promoted the shoot elongation. In addition. common milkweed was sensitive to culture temperature in vitro. Temperature around 22$^{\circ}C$ was favorable for shoot multiplication and growth, whereas temperature higher than $25^{\circ}C$ usually reduced the rate of shoot survival rate.