• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2019.10a
• /
• pp.29-29
• /
• 2019

상동나무(Sageretia theezans)는 갈매나무과 상동나무속 식물로 특이하게 늦가을에 꽃이 피고 봄에 열매가 익는 수종이며, 최근 암세포 생장억제 효과가 입증되고 제주와 전남 남해안 지역에서 예로부터 열매를 식용 및 약용하여 민속식물로서의 가치가 크다. 본 연구에서는 향후 약용소재로서의 가치가 높은 상동나무의 대량증식을 위하여 식물생장호르몬의 처리에 따른 효과적인 증식법을 구명하고자 실시하였다. 상동나무 삽수묘는 고흥, 완도 일대에서 2019년 3월 19일에 2년생 가지를 수집하였으며, 3월 20일 삽목을 실시하였다. IAA(250, 500, 1,000 ppm), IBA(250, 500, 1,000 ppm), NAA(250, 500, 1,000 ppm), 루팅(200배) 수용액에 50개의 삽수묘를 3분간 절단기부를 침지 후 모래에 치상하였으며, 무처리구는 바로 치상하였다. 각 삽수상은 온실에 보관 후 1일 2회씩 관수 하였으며 120일간 유지하였다. 그 결과 전체 66.4%의 발근율을 보였고 캘러스와 뿌리의 발달은 평균 17.5 mm의 길이를 보였다. IBA 처리구가 전반적으로 높은 발근율을 보였는데 그 중 500 ppm 처리구에서 가장 많은 41개 삽수묘의 부정근이 형성되어 82%의 발근율을 보였고, 평균 20.2 mm 길이의 뿌리가 생성되었다. IBA 1,000 ppm처리구에서도 38개의 삽수묘에서 캘러스 및 부정근이 형성되어 76%의 발근율을 보였다. 뿌리의 발달은 평균 18.4 mm로 IBA 500 ppm에 비해 길이가 짧았으나 뿌리의 길이가 삽수묘간 차이가 커 가장 긴 개체가 32.3 mm로 나타났다. NAA 500 ppm에서도 높은 발근율을 보였는데 32개의 삽수묘에서 부정근이 형성되어 64%의 발근율로 나타났다. 뿌리의 길이는 18.9 mm로 IBA 1,000 ppm에 비해 발근율은 낮았으나 뿌리의 발달은 평균 0.5 mm 높은 것으로 나타났다. 무처리구는 29개의 삽수묘가 캘러스 및 부정근이 형성되었으며 58%의 발근율로 나타났으며 IAA, IBA, NAA 처리구 모두 무처리구에 비해 높은 발근율을 보였다. 생장호르몬 당 발근율을 보면 IBA는 500 ppm, NAA는 500 ppm, IAA는 1,000 ppm에서 가장 높은 발근율을 보였다. 루팅 수용액 처리구는 60%의 발근율을 보여 루팅 200배 수용액 침지와 무처리는 비슷한 수치의 발근율로 나타나 루팅은 상동나무 삽목 발근촉진제로 효과가 없는 것으로 확인되었다.

• Horticultural Science & Technology
• /
• v.16 no.1
• /
• pp.33-36
• /
• 1998

This study was carried out to examine the effects of cutting dates and rooting promoters on rooting of the Rhododendron mucronulatum Turcz. The rooting percentage in greenwood cutting was high in June and decreased after July 3, when reproductive growth began. The best rooting percentage(92.5%) occurred when cutting was completed on June 12. There was no difference in rooting potentials between the greenwood straight cutting and the greenwood heel cutting. Cuttings of R. mucronulatum for. albiflora showed high rooting percentage(85%) similar to the cuttings of R. mucronulatum. Dipping cuttings in NAA $2,000mg{\cdot}L^{-1}$ solution for 15 sec increased the rooting percentage up to 97.5%, and also promoted root growth.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.12 no.1
• /
• pp.39-45
• /
• 2010

This study was carried out to investigate the growth and rooting of cutting time, media and plug cell size in Dianthus spp and Coreopsis spp. A rooting rate was the low in lated rooting time rooting rate was the over 90% in 20th May and 20th August otherwise was the 82% and 87% in 20th September. Media of rooting was the over the 90% in all treatment media and sand media was low. Number of rooting was the best in peatmoss(1)+perlite(1) media. The size of plug cell was the 98% in 162 plug cell in Coreopsis spp. The 162-200 plug cell size was the 95.8-96% in Dianthus spp.

• Korean Journal of Environment and Ecology
• /
• v.29 no.6
• /
• pp.842-849
• /
• 2015

This study investigated the effects of rare-earth fertilizer on the shoot cuttings' rooting of Vitex rotundifolia L. and Tamarix chinensis Lour. The shoot cutting test was carried in 2008 and the main results are summarized as follows. The rate of rooting and the average roots increased in both number and length when rare-earth fertilizer is treated in V. rotundifolia and T. chinensis in comparison to those of the untreated control plot. In particular, when rare-earth fertilizer is diluted with water 1/2500, the rooting outstandingly increases. This result is almost similar to the effect of the rooting stimulant, IAA. Although there is no differentiation in its rooting rate according to the density, the rooting of T. chinensis shows a 100 percent effect on in the entire treated plot but not in the untreated control plot, so it is usable as a rooting stimulant. As for shoot cuttings' rooting, depending on the time immersed in diluted solution of rare-earth fertilizer, both V. rotundifolia and T. chinensis showed relatively higher percentages in all treatment plot immersed for 60 minutes than for 10 minutes. In conclusion, considering the results of the rooting percentage and the average number and length of roots of V. rotundifolia and T. chinensis, the shoot cuttings' rooting appeared higher in percentage when they were immersed in the rooting stimulant for sixty minutes with a lower density than 1/2500. This result shows that rare-earth fertilizer can be utilized as an alterative for IAA rooting stimulants currently available in the market.

• The Journal of Natural Sciences
• /
• v.6 no.1
• /
• pp.55-64
• /
• 1993

Experiments were conducted to study the effect of auxins and cultural factors on rooting characteristics in offshoots cutting of Aloe vera. The result obtained were as follows;1. Short offshoots(15-20cm) with rhizome had more roots than that of the removed rhizome, while long offshoots(25-30cm) without rhizome had more roots and heavier root dry weight than short offshoots with rhizome. The highest rooting ability occured when IBA treated 500mg per liter in short offshoots with rhizome, whereas long offshoots without rhizome showed higher rooting ability at the level of 1,000mg/l of IBA and 200mg/I of NAA2. Among three kinds of anxin treated, both IBA and NAA showed better rooting ability than ethychlozate. Root number, root length and root dry weight were higher at 10 min. dipping treatment of IBA 500mg/I for short off-shoots without rhizome and 10 min. dipping treatment of IBA 1,000mg/l for long offshoots with rhizome. Rooting ability of long offshoots was higher than that of short offshoots.3. In addition to IBA 1,000mg/l, 7-day dryness under shading condition increased root number and dry weight of root in offshoots without rhizome. Control treatment lengthened root by increasing drying period under the shading condition.4. The plain soil with mamure had not only higher root dofferentiation but higher root growth of offshoots cutting than the artificial soil.5. Rooting ability in offshoots cutting had a tendency increasing with decrease in shading percentage to natural. Black color poly ethylene film mulching had better rooting ablility than transparency poly ethlene film mulching in offshoots cutting.

• FLOWER RESEARCH JOURNAL
• /
• v.17 no.4
• /
• pp.297-302
• /
• 2009

This study was carried out to investigate the characteristics of rooting and bud break of single-stemmed roses (Rosa hybrida L. 'Rate Rose' and 'Teresa') as affected by axillary bud position and leaf area of cuttings derived from different growth stage of the mother shoots. In fact, both rooting and budbreak of single-stemmed roses were not influenced by growth stage of mother shoots, with showing more than 95% in all treatment related to mother shoot maturity. 'Rote Rose' required 34 days to average rooting and budbreak after cutting, whereas 'Teresa' did only 18 days to budbreak which was 9 days faster than rooting. Rooting and bud break needed more time and showed lower percent as the axillaty bud position for cuttings went down to the base of mother shoots. Especially 'Teresa' showed 12 days of delay to budbreak and 14.4% decrease in budbreak. Also, the increase in leaf area of cuttings accelerated rooting and budbreak, of which the time was shorter and the percent was higher.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.17 no.1
• /
• pp.145-155
• /
• 2015

To investigate the practicality of a small scale softwood propagation plastic film tent and the rooting promoting effect of indole-3-butyric acid(IBA) for northern highbush blueberry, softwood cuttings of 3 varieties (Duke, Elizabeth, Elliot) were made after dipping in the IBA solutions of 100ppm, 300ppm, 500ppm, 1,000ppm for 2 hour, and 3,000ppm for 20 seconds, respectively. Two-way ANOVA confirmed the significant differences in the percentage of rooting of cutting, longest root length and rooting volume index (RVI) induced by IBA treatment, as well as the percentage of rooting of cutting and RVI among examined varieties. In particular, the highly significant difference was observed in th RVI for IBA treatment. However, there was no interactions between the auxin treatments and varieties. Spontaneous rooting ability (control) depends on the varieties, showing that the lowest rooting ability was observed in the 'Duke' (45%) followed by 'Elliot'(85%) and 'Elizabeth' (90%). In addition, the rooting of the examined 3 varieties were enhanced by both of short time (20sec) dipping treatment of low level solutions, and long time (2hr) dipping treatment of high level solution, with the remarkably promoted rooting of the 'Duke' cuttings.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2020.08a
• /
• pp.44-44
• /
• 2020

본 연구는 최근 암세포 생장억제 효과가 입증되어 약용소재로서의 활용가치가 높은 상동나무 (Sageretia theezans)의 대량증식 연구의 기반을 마련하기 위해 삽수묘를 시기별로 채취하여 삽목실험을 실시하였다. 삽수묘는 제주 대정읍, 전남 완도 보길도, 청산도 인근에서 수집하였으며, 상동나무 삽목은 시기별로 2019년 3월 21일(춘계), 7월 22일(하계), 9월 2일(추계)에 실시하였다. 토양조건은 모래, 원예용 상토, 모래와 원예용 상토를 1 : 1 배율로 섞은 토양에 각각 치상하였다. 삽목 후 산림약용자원연구소 온 실에 약 100일간 보관하였으며 1일 2회 관수하여 관리하였다. 그 결과 춘계에 삽목한 삽수묘에서 66.4% 의 발근율로 나타났으며, 뿌리는 평균 17.5mm의 길이로 측정되었다. 하계는 27.4%의 발근율을 보였으 며, 뿌리는 평균 12.6mm의 발달을 보였다. 추계에는 29.6%의 발근율로 13.2mm의 뿌리 발달을 보였다. 상동나무는 춘삽이 추삽에 비해 발근율이 높고, 숙지삽이 상동나무 발근에 있어 유리한 것으로 사료된 다. 또한 토양은 모래에 삽목한 삽수묘가 60.3%로 가장 발근율이 높았으며, 그 다음으로 모래와 원예용 상토를 1 : 1 배율로 섞은 토양이 42.7%의 발근율을 보였다. 마지막으로 원예용 상토에서 25.4%의 발근 율을 보였다. 이는 상동나무 삽목시 배수가 양호한 상태가 뿌리 및 캘러스형성에 유리하게 작용하는 것 으로 사료된다.

• Journal of Korean Society of Forest Science
• /
• v.83 no.2
• /
• pp.205-210
• /
• 1994

This study was conducted to develop a method of mass production by cutting of superior genotypes of Larix leptolepis and to examine the effects of ortet age, clone, crown position, and an applied plant growth substance upon the rooting of cuttings. Four different ortet ages, 2, 8, 16 and 30 years and three levels of root-promoting substances, indole butyric acid(IBA), at 1000, 2000, and 5000ppm were employed. Greenwood cuttings were taken from mother trees in late July and rooted for three months in rooting medium containing peatmoss : vermiculite : perlite(1 : 1 : 1 by volume). with temperature controlled at $20-25^{\circ}C$, humidity by intermittent misting and light by partial shading in the greenhouse. Cuttings treated with 1000ppm IBA showed highest rooting, with 80%, 71%, 52%, 25% for 2-, 8-, 16-. 30-year-old ortet, respectively. Untreated cuttings showed rooting of 52%, 48%, 36%, 20% for the ortet age of 2, 8, 16, 30 years, respectively. The average number of adventitious roots decreased with increasing ortet age from 2 to 30 years, whereas IBA treatment increased the number of roots in all ages. A variation in rooting among 20 clones tested was observed. The 4 clones, Chungnam 6, and 7 and Chonbuk 1, and 9 showed good rooting of about 67%, while Kangwon 49 showed boor rooting of 40% at 1000ppm IBA treatment. When crown position was compared, cuttings taken from middle crown showed highest rooting of 60%, while cuttings from upper crown showed lowest rooting of 53%.

• Korean Journal of Plant Resources
• /
• v.15 no.3
• /
• pp.269-278
• /
• 2002

To establish the mass propagation methods of Weigela subsessilis,, a promising native plant species for horticultural use, several factors influencing rooting from stem cuttings were evaluated. Softwood cuttings showed best rooting rate, 70~77%, in all the cutting media tested, 2.8 times more than hardwood cuttings in which perlite was best medium. The rooting from hardwood cuttings was promoted by higher concentration of all growth regulators used,500 mg.L$^{-1}$ NAA being the best with 80%. More than 80% of softwood cuttings treated by growth regulators produced roots, especially 100, 500, and 1000mg.L$^{-1}$ NAA producing 97％. Roofings from semi-wood cuttings was enhanced by all the growth regulators, except 1000 mg.L$^{-1}$ concentration in which rooting was reduce. Higher rooting rate was obtained by higher concentration and longer soaking duration of NAA in case of hardwood cutting. Softwood cutting showed 100 ％ rooting by soaking treatment with 500 mg.L$^{-1}$ NAA for 3 min. Rooting rate of semi-hardwood cuttings was promoted by higher concentration and longer soaking with growth regulators, However, the degree of improvement was lower than soft and hardwood cutting.