• The KIPS Transactions:PartB
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• v.15B no.6
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• pp.561-574
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• 2008

The spot detection phase of the 2-DE image analysis program segments a gel image into spot regions by an image segmentation algorithm and fits the spot regions to a spot shape model and quantifies the spot informations for the next phases. Currently the watershed algorithm is generally used as the segmentation algorithm and there are the Gaussian model and the diffusion model for the shape model. The diffusion model is closer to real spot shapes than the Gaussian model however spots have very various shapes and especially an asymmetric formation in x-coordinate and y-coordinate. The reason for asymmetric formation of spots is known that a protein could not be diffused completely because the 2-DE could not be processed under the ideal environment usually. Accordingly we propose an asymmetric diffusion model in this paper. The asymmetric diffusion model assumes that a protein spot is diffused from a disc at initial time of diffusing process, but is diffused asymmetrically for x-axis and y-axis respectively as time goes on. In experiments we processed spot matching for 19 gel images by using three models respectively and evaluated averages of SNR for comparing three models. As averages of SNR we got 14.22dB for the Gaussian model, 20.72dB for the diffusion model and 22.85dB for the asymmetric diffusion model. By experimental results we could confirm the asymmetric diffusion model is more efficient and more adequate for spot matching than the Gaussian model and the diffusion model.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.1 no.1
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• pp.23-26
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• 2000

In spot welded joint. Electronic Speckle Pattern Interferometry(ESPI) method using the Model 95 Ar laser a video system and an image processor was applied to measure the stress Unlike traditional strain gauges or Moire method, ESPI method has no special surface preparation or attachments and can be measured in-plane displacement with non-contact and real time. In this experiment, specimens are loaded in parallel with a load cell. The specimens are made of the cold rolled steel sheet with 1mm thickness, are attached strain. gauges. This study Provides an example of how ESPI has been used to measure stress and strain inspecimen. The results measured by ESPI are compared with the data which was measured by strain gauge method under tensile testing.

• Korean Journal of Agricultural Science
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• v.6 no.1
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• pp.27-32
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• 1979

Seed samples of sesame (Sesamum indicum L.) from Chung-nam Provincial Office of Rural Development were infected by 12 fungi. Alternaria sesamicola, A. tenuis and Corynespora cassicola were the predominant fungi. Except C. cassicola, all fungi were almost completly reduced and wipid out the infections by pretreatment with chlorine. Plating of seed components also indicate that C. cassicola was well-established infections. In infection experiment C. cassicola produced severe leaf and stem spots and blights on sesame plants and showed severe seed and seedling rot.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.16 no.3
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• pp.123-128
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• 2016

In manufacturing processing of a secondary battery, the visual inspection system is studied and developed to check the surface defects of the electrode plates. It consists of two parts, one is the hardware control and the other software implementation. The former is made up to the system configuration and the design of the optical system, the illuminations and the controllers. The latter is the detection algorithms of the surface defects. This system achieves the quality improvement of the electrode process and the price competitiveness. By using the proposed defects detection algorithms this system demonstrates the high reliability of spot, line, manhole, extraneous substance, scratch, and crater defect of a electrode plate surface.

• Korean Journal of Agricultural Science
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• v.12 no.2
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• pp.199-205
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• 1985

1. Alternaria tenuis, Cladosporium cladosporioides, Curvularia lunata Fusarium semitectum, Gerlachia oryzae, Phoma sp. Trichoconiella padwickii were found associated with ear blighting of rice plants. The frequency of isolation of the fungi was found to be higher from the severely discolored rice grains than that from the brownish spotted grains, but H. oryzae was encounted highly from the latter. 2. Higher counts of Alternaria tenuis, Helminthosporium oryzae, Trichoconiella padwickii, Fusarium moniliforme, Fusarium semitectum were observed under alternating cycles of 12hrs of light and darkness than under complete darkness but G. oryzae was encountered highly under darkness in the blotter. While no difference in the occurrence of the other fungi was recorded under l2hr NUV light-12hr darkness cycles and darkness. 3. A. tenuis and C. cladosporioides were encountered highly at the lower temp. $(15^{\circ}C)$ but Phoma sp. was occurred highly at the higher temp. $(30^{\circ}C)$ when the seeds were incubated in the blotter. 4. Artificial inoculation of the fungi in the ears of rice plants caused severe discoloration of rice grains and reduced 1,000 grain weights and grain ripening percentage.

• Journal of fish pathology
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• v.21 no.1
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• pp.21-27
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• 2008

Mass mortality occurred in mud loaches, Misgurnus mizolepis, cultured in ponds located in Kunsan. External signs of affected fish showed hemorrhage of skin and fins, Internally, pale liver with congestion, enlarged kidney, and spleen and enteritis exhibited. Causative bacteria isolated from liver, spleen, and kidney of the disease fish. In biochemical tests, the isolates were similar with those of the reference strains, A. sobria. The aerolysine gene from the present isolate was amplified PCR with the primer SOBF and SOBB for A. sobria. The isolate was identified as A. sobria on the basis of those tests. In virulence test, the present isolate resulted in the development of clinical signs identical to those in naturally infected fish. The present results conclude that the present isolate is A. sobria and can be a pathogen which causes motile aeromonad septicemia to mud loach.

• Journal of the Korea Institute of Military Science and Technology
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• v.4 no.1
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• pp.198-206
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• 2001

The structure of Yb complex with 3-nitro-1,2,4-triazol-5-one(NTO), $[Yb(NTO)3(H2O)4].5H2O$ has been investigated by X-ray diffraction method. Crystallographic data for the title compound : monoclinic, C2/c, $a=36.925(2){\AA},$ b=6.6770(4)${\AA},$ c=25.6376(15)${\AA},$ {\beta}=130.978(1)^{\circ},$ V=4772.0(5)${\AA}^3,$ Z=8, $D_c,=1.952\; Mg/m^3.$ The intensity data were collected on a Broker SMART diffractometer equipped with a CCD area detector using Mo $K\alpha$ radiation. The structure was solved by direct method and refined by full-matrix least-squares calculations to a final R value of 0.0424 for 4727 independent reflections and 335 parameters. The three carbonyls of three NTO anions and four ligand water molecules which are formed the pentagonal bipyramid are coordinated with $Yb^{3+}$ and also five water molecules are included in the form of the crystal water in the molecular structure.

• Journal of the Institute of Convergence Signal Processing
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• v.9 no.1
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• pp.1-9
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• 2008

One of the problems for implementing the spot detection phase in the 2-DE gel image analysis program is the eliminating noises in the image. Remained noises after the preprocessing phase cause the over-segmented regions by the segmentation phase. To identify and exclude the over-segmented background regions, if we use the fixed thresholding method that is choosing an intensity value for the threshold, the spots that is invisible by the eyes but mean a very small amount proteins which have important role in the biological samples could be eliminated. This paper propose an adaptive thresholding method that come from an idea that is got on statistical analysing for the prominences of the peaks. The adaptive thresholding method works as following. Firstly we calculate an average prominence value curve and fit it to exponential function curve, as a result we get parameters for the exponential function. And then we calculate a threshold value by using the parameters and probability distribution of errors. Lastly we apply the threshold value to the region for determining the region is a noise or not. According to the probability distribution of errors, the reliability is 99.85% and we show the correctness of the proposed method by representing experiment results.

• Research in Plant Disease
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• v.17 no.3
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• pp.358-363
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• 2011

Dapple fruits of plum cv. Oiishiwase (Prunus salicina L.) were occurred at Gyeonggi-do and Gyeongsangbukdo. The symptoms resembled the dapple fruit disease caused by Hop stunt viroid (HSVd). To identify the causal disease agents, RT-PCR was performed with the specific primers of HSVd. RT-PCR analysis showed that HSVd variants (DP1, DP2) were detected from dapple fruits. HSVd detection was also confirmed by the dot blot hybridization using a DIG-probe specific to HSVd. Nucleotide sequences of DP1 and DP2 had the identities of 94-100% with those of other 7 variants of HSVd in Genbank database. DP1 and DP2 were different in two nucleotides of CG and AA at position of 59 and 60, orderly. Based on nucleotide sequences at position of 59 and 60, HSVd variants associated with plum dapple fruits could be divided mainly into three groups as CG, AA and TG.

• Research in Plant Disease
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• v.19 no.3
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• pp.183-187
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• 2013

Barley kernel discoloration (KD) leads to substantial loss in value through downgrading and discounting of malting barley. The objective of this research is to investigate fungal distribution and varieties resistance to KD in Korean two-rowed barley. Several fungal organisms including Alternaria spp., Fusarium spp., Aspergillus spp., Epicoccum spp. and Rhizopus spp. were isolated from Korean two-rowed barley representing KD. The symptoms of KD were brown and black discolorations of the lemma and palea. The most frequently detected fungal species was Alternaria spp. which exhibited 69.1% and 72.2% in 2011 and 2012, respectively. Epicoccum spp., Fusarium spp., and Aspergillus spp. were also detected. Fusarium spp., primary pathogen of barley head blight, were rarely occurred in the 2011 and their occurrence increased to 4.7% in 2012. Twenty cultivars of Korean two-rowed barely were evaluated to KD. The average percentage of KD was 8.0-36.0% in 2011 and 5.2-36.6% in 2012. Two cultivars ('Sacheon 6' and 'Dajinbori') showed KD of 6.2% to 8.8% and determined resistant, however 'Samdobori' and 'Daeyeongbori' demonstrating KD of 22.2-36.6% were highly susceptible. 'Jinyangbori', 'Danwonbori', 'Sinhobori' and 'Kwangmaegbori' showing KD of less than 15% were moderately resistant cultivar.