• 제목/요약/키워드: 반응 조건 최적화

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Preparation Of levan Oligosaccharides by Acid Hydrolysis and It Application in Growth of lactic Acid-producing Bacteria (산가수 분해법에 의한 레반 올리고당의 제조 및 유산군 생육촉진 효과 연구)

  • 강태호;정성제;강순아;강기효;장은경;김승환;김철호;이상기;전억한
    • KSBB Journal
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    • v.17 no.2
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    • pp.137-141
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    • 2002
  • Levan oligosaccoharides and low molecular weight levin were produced from levin by acid hydrolysis and following column chromatography. Levan hydrolysis was progressed proportionally as increased incubation time. In terms of levan hydrolysis reaction, no differences were found from the sources of levan. Optimum hydrolysis conditions for the formation of levan oligosaccharides were, 0.38 M H$_2$S0$_4$; and incubation at 95$\^{C}$ for 4 min. The purified products were determined as the mixture of oligosaccharides (degree of polymerization (DP) of 3-6), Two of lactic acid-producing bacteria, Lactobacillus plantarum KCTC 3104 and Pediococcus pentosaceus KCTC 3507, were studied in vitro for their ability to metabolize levin oligosaccharides. Apparently, the growth of both cells were increased by levin oligosaccharide diet, compared with those of levan diets, suggesting that levan oligosaccharides may be beneficial in selectively growth of lactic acid-producing bacteria.

A Study of Scattered Radiation Effect on Digital Radiography Imaging System (디지털 방사선영상 시스템에서 산란선이 영상 품질에 미치는 영향)

  • Baek, Cheol-Ha
    • Journal of radiological science and technology
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    • v.40 no.1
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    • pp.71-78
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    • 2017
  • Scattered radiation is inherent phenomenon of x-ray, which occurs to the subject (or patient). Therefore it cannot be avoidable but also interacts as serious noise factor because the only meaningful information on x-ray radiography is primary x-ray photons. The purpose of this study was to quantify scattered radiation for various shooting parameters and to verify the effect of anti-scatter grid. We employed beam stopper method to characterize scatter to primary ratio. To evaluate effect on the projection images calculated contrast to noise ratio of given shooting parameters. From the experiments, we identified the scattered radiation increases in thicker patient and smaller air gap. Moreover, scattered radiation degraded contrast to noise ratio of the projection images. We find out that the anti-scatter grid rejected scattered radiation effectively, however there were not fewer than 100% of scatter to primary ratio in some shooting parameters. The results demonstrate that the scattered radiation was serious problem of medical x-ray system, we confirmed that the scattered radiation was not considerable factor of dig ital radiog raphy.

Characterization of Reverse Osmosis Membrane Surface Modified by Silane-epoxy Using UV (UV를 적용한 역삼투막의 실란-에폭시 표면 개질 및 특성 평가)

  • Park, Hee Min;Yang, Won Yong;Lee, Yong Taek
    • Membrane Journal
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    • v.28 no.3
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    • pp.169-179
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    • 2018
  • The purposes of this paper were to improve both fouling and chlorine resistance by increasing the hydrophilicity of the reverse osmosis membrane. In order to improve chlorine resistance, the surface of RO membrane was activated by ultraviolet irradiation, and then it was modified by the sol-gel method using Octyltriethoxysilane (OcTES) such as the silane coupling agent to low sensitivity to chlorine, thereby the polyamide active layer was protected and chlorine resistance was improved. In addition, polyglycerol polyglycidyl ether (PGPE) and sorbitol polyglycidyl ether (SPE) coating with different number of epoxides, ring opening reaction of epoxide improved the anti-fouling resistance. The surface modification condition was optimized by FT-IR, XPS, and contact angle analysis. As a result, the permeability reduction rate of the silane-epoxy modified membrane after the fouling test was decreased about 1.5 times as compared with that of the commercial membrane. And the salt rejection was maintained over 90% at $20,000ppm{\times}hr$ even after chlorine resistance test.

Optimization of the High-Pressure Condition for Rice Protein Extracting Using Response Surface Methodology (RSM) (반응표면분석법을 이용한 쌀 단백질 초고압 추출조건 최적화)

  • Ra, Ha-Na;Park, Sa-Ra;Kim, Ha-Yun;Cho, Yong-Sik;Kim, Kyung-Mi
    • Journal of the Korean Society of Food Culture
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    • v.34 no.6
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    • pp.779-784
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    • 2019
  • The purpose of this study was to optimize the rice protein extracted using a response surface methodology. The experiment was designed based on a CCD (Central Composite Design), and the independent variables were the high pressure (X1, 0-400 MPa) and processing time (X2, 0-10 minutes). The results of the extraction content (Y1), residue content (Y2), and recovery yield (Y3) were fitted to a response surface methodology model (R2= 0.92, 0.92, and 0.93, respectively). Increasing the pressure and processing time has a positive effect on the extraction content (Y1), residue content (Y2), and recovery yield (Y3). Therefore, these high-pressure conditions (independent variables) can significantly affect the improvement in rice protein extraction efficiency. Thus, the optimal conditions of X1 and X2 were 400 MPa and 10 min., respectively. Under these optimal conditions, the predicted values of Y1, Y2, and Y3 were 62.93, 57.53 mg/g, and 91.76%, respectively.

Optimum Condition for Non-Aqueous Dispersion Polymerization of Environmentally-friendly Acrylic Resin (비수계 분산중합에 의한 환경친화적 아크릴 수지 합성의 최적화 연구)

  • Oh, Dae Geun;Lee, Kyung Hoon;Kim, Wan Tae;Min, Byung Hoon;Chung, Dae-won;Lee, Jong Doo;Kim, Jeong Ho
    • Applied Chemistry for Engineering
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    • v.19 no.4
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    • pp.370-375
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    • 2008
  • Optimum synthesis conditions were investigated for the non-aqueous dispersion (NAD) polymerization of environmentally-friendly acrylic resin. The optimum amount of stabilizer was observed to be 20 wt% to prevent flocculation of the synthesized polymer particles. The optimum ratio of aliphatic solvents to aromatic solvents turned out to be 80 to 20 in order to obtain a stabilized NAD resins. The viscosity of NAD resins was affected not only by the reaction time but also by the amount of initiator and the impeller rpm. Also, the particle size and the size distribution were influenced by the amount of stabilizers and initiators. Since the stability of NAD resin was devastated by the increase in particle size when all the monomers were fed at the beginning of polymerization, the monomers need to be put into the reactor step by step throughout the entire polymerization process.

Electrochemical Destruction of Cyanide Ions and Recovery of Zinc Ions from Electroplating Wastewater (도금폐수 중의 시안착이온의 전기화학적 분해 및 아연 회수에 관한 연구)

  • Niu, Lin;Ro, Byung-Ho;Jung, Cheul;Lee, Yong-Ill
    • Analytical Science and Technology
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    • v.13 no.6
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    • pp.699-704
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    • 2000
  • A study has been made for the electrochemical destruction of cyanide ions and removal of zinc ions from a simulated electroplating wastewater by the use of a platinum platized-titanium anode and a stainless steel cathode. Several experimental parameters, including electrolysis time, cell current, additives, and chloride concentration, have been investigated and used for efficient destruction of cyanide waste and removal of zinc ions from aqueous solutions. It was found that cell current and type of additives gave great effects on the destruction of cyanide ions and removal of zinc ions. The optimized conditions (electrolysis time: 1hr, current: 12A, additive: 0.5 M NaCl) have been defined to destroy cyanide ions and remove zinc ions with high efficiency and low operation cost. The proper reaction mechanism leading to the destruction of cyanide on the anode has also been discussed.

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Classification of Capsicum annuum Germplasm Using Random Amplified Polymorphic DNA (RAPD를 이용한 고추(Capsicum annuum) 유전자원의 분류)

  • Nam, Seung-Hyun;Choi, Geun-Won;Yoo, Il-Woong
    • Horticultural Science & Technology
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    • v.16 no.4
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    • pp.503-507
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    • 1998
  • This study was initiated to evaluate genetic relationship among various domestic and exotic pepper accessions using random amplified polymorphic DNA(RAPD) markers. The results suggested that the optimum conditions for PCR with random primers in Capsicum spp. could be obtained with 3mM of $MgCl_2$, 1.5U of Taq. DNA polymerase, 10ng of template DNA, $200{\mu}M$ of dNTPs, 200nM of random primer, and $42^{\circ}C$ of annealing temperature. Sixteen random primers showing high band intensity and reproducibility were selected from 80 random primers. Primers having 70% GC content were more effective in DNA amplification than primers having 60% GC content. The total 93 DNA bands including 71 polymorphic bands and 22 monomorphic bands were obtained with selected 16 random primers for 31 pepper cultivars and lines. About 4.4 polymorphic bands per primer were produced. Similarity coefficients were calculated by using 71 polymorphic bands and dendrogram based on the similarity coefficient showed clear classification of 31 peppers into three Capsicum species of Capsicum annuum, Capsicum chinense and Capsicum chacoense.

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Optimization of Preparation Conditions of Houttuynia cordata Beverage Containing Saururus chinensis and Polygonatum odoratum by Sensory Evaluation and Response Surface Methodology (관능 평가 및 반응 표면 분석에 의한 삼백초와 둥굴레 가미 어성초 음료의 제조 조건 최적화)

  • Seung, Suk-Kyung;Chang, Kyung-Ho;Joung, Kyung-Hee;Kim, Ae-Jung
    • Journal of the East Asian Society of Dietary Life
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    • v.18 no.6
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    • pp.1072-1080
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    • 2008
  • The optimization of the addition ratios of Houttuynia cordata, Saururus chinensis, and Polygonatum odoratum var. pluriflorum hot water extract to Houttuynia cordata beverage was evaluated by applying response surface methodology. The quality characteristics of a selected best product were investigated. Sweet, savory, and fishy taste, and overall acceptability were optimized by response surface methodology using a central composite design. The concentration of effect of Houttuynia cordata, Saururus chinensis, and Polygonatum odoratum var. pluriflorum hot water extract was modeled using an equation. Hot water extracts of Houttuynia cordata ($1.75{\sim}1.89%$), Saururus chinensis ($1.03{\sim}1.04%$), and Polygonatum odoratum var. pluriflorum ($2.17{\sim}2.31%$) represented the optimal concentration conditions to obtain the best taste. The physical and chemical characteristics of the beverage manufactured at optimized conditions were pH 4.18, soluble solids 0.41 $^{\circ}Brix$, total acidity 0.21%, color value ($L^*$ value 40.08, $a^*$ value 4.53, and $b^*$ value 10.69), total polyphenol 54.63 mg/L, and electron donating ability 26.98%.

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A Study on the Optimization of Combustion and Emission Performance in a Heavy-duty HCNG Engine (Heavy-duty HCNG엔진의 연소 및 배기성능 최적화에 관한 연구)

  • Choi, Young;Park, Chul-Woong;Won, Sang-Yeon;Kim, Chang-Gi
    • Journal of the Korean Institute of Gas
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    • v.15 no.2
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    • pp.15-20
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    • 2011
  • Although CNG is able to meet the current emission standards, it is expected to be impossible to satisfy the requirements of the next EURO-6 emission regulation without an additional after-treatment device. Hydrogen is known to be a gaseous fuel which features the wide flammability limit and the fast reactivity. A certain amount of hydrogen addition to CNG is able to extend the lean combustion range and produce lesser amounts of harmful emissions. In this research, the combustion and emission characteristics of HCNG(mixture of Hydrogen and CNG) fuel were experimented in an 11-liter heavy duty lean burn engine varying hydrogen contents, air-to-fuel ratio and spark timing. The optimization of this HCNG engine for a city bus was performed through the evaluations of oxidation catalyst characteristics.

Optimization for Enzymatic Hydrolysis of Mannitol (만니톨의 효소 가수분해 반응 조건 최적화)

  • Park, Eun-Young;Kim, Yong-Jin;Jeong, Seung-Mi;Lee, Dong-Hoon
    • KSBB Journal
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    • v.28 no.2
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    • pp.65-73
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    • 2013
  • This study aimed to investigate the enzymatic hydrolysis of mannitol using Viscozyme$^{(R)}$ L, Celluclast$^{(R)}$ 1.5 L, Saczyme$^{(R)}$, Novozym$^{(R)}$, Fungamyl$^{(R)}$ 800 L, Driselase$^{(R)}$ Basidiomycetes sp., and Alginate Lyase, and to optimize of reaction conditions for production of reducing sugar. Response surface methodology (RSM) based on central composite rotatable design was used to study effects of the independent variables such as enzyme (1-9% v/w), reaction time (10-30 h), pH (3.0-7.0) and reaction temperature ($30-70^{\circ}C$) on production of reducing sugar from mannitol. The coefficient of determination ($R^2$) of $Y_1$ (yield of reducing sugar by Viscozyme$^{(R)}$ L) and $Y_3$ (yield of reducing sugar by Saczyme$^{(R)}$) for the dependent variable regression equation was analyzed as 0.985 and 0.814. And the p-value of $Y_1$ and $Y_3$ showing 0.000 and 0.001 within 1% (p < 0.01), respectively, was very significant. The optimum conditions for production of reducing sugar with Viscozyme$^{(R)}$ L were 9.0 % (v/w) amount of enzyme, 30.0 hours of reaction time, pH 4.5 and $30.0^{\circ}C$ of reaction temperature, and those with Saczyme$^{(R)}$ were 9.0% (v/w) of amount of enzyme dosage, 30.0 h of reaction time, pH 7.0 and $30.0^{\circ}C$ of reaction temperature, consequently, the predicted reducing sugar yields were 22.5 and 27.9 mg/g-mannitol, respectively.