• 제목/요약/키워드: 반딧불이

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Toxicity of Firefly, Luciola lateralis(Coleoptera: Lampyridae) to Commercially Registered Insecticides and Fertilizers (애반딧불이(Luciola lateralis)에 대한 살충제와 비료의 독성평가)

  • Lee, Ki-Yeol;Kim, Young-Ho;Lee, Jae-Wung;Song, Myung-Kyu;Nam, Sang-Ho
    • Korean journal of applied entomology
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    • 제47권3호
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    • pp.265-272
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    • 2008
  • Toxicity of 10 registered insecticides and 6 fertilizers were tested against $3^{rd}$ larva and adults of Korean firefly, Luciola lateralis Motschulsky(Coleoptera: Lampyridae). All experiments were tested at the recommended concentration of each inescticides by producer. MEP, PAP, Acephate, Fenthion, and Diazinon, which were organophates, a mixtures combined with Burofezin fenobucarb, Cartap buprofezin, and Thiamethoxam(Neonicotinoids), Fipronil(Phenylpyrazoles) showed more 80.0% mortality on larva and adults of L. lateralis. However, tebufenozide(I.G.R) showed low mortality of 33.3%. $LC_{50}$ (ppm) value of Assit, Cartap buprofezin, Fenthion and PAP were showed 1.03 ppm, 1.90 ppm, 10.26 ppm, 0.98 ppm, respectively, against $3^{rd}$ larva of L. lateralis. Effects against eggs showed very high toxicity. Otherwise, tebufenozide(I.G.R) was showed hatchability of 100%. Toxicity of Urea fertilizer, Ammonium sulfate, Potassium chloride, Fused phosphate, Complex fertilizer and Silicate fertilizer were showed the mortality with 27.3%, 56.7%, 73.3%, 0.0%, 0.0%, 0.0%, respectively, when exposed 72 hrs after treatment.

Seasonal Species Composition of Marine Organism Collected by a Shrimp Beam Trawl in Nakdong River Estuary, Korea (낙동강 하구에서 새우조망으로 채집된 생물의 계절별 종 조성)

  • Lee, Jong Hee;Lee, Jae Bong;Kim, Jung Nyun;Lee, Dong Woo;Shin, Young Jae;Chang, Dae Soo
    • Korean Journal of Ichthyology
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    • 제21권3호
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    • pp.177-190
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    • 2009
  • Species composition and abundance of marine organism in the Nakdong River estuary were investigated seasonally from November 2007 to November 2008. During the study period, a total of 169 species, 93 families, and 6 taxa were collected in the study area. Species included were 2 species in Bivalvia, 11 in Cephalopoda, 43 in Crustecea, 8 in Echinodermata, 6 in Gastropoda, and 99 in Pisces. The seasonal dominant species in number of individuals were Acropoma japonicum in autumn, Apogon lineatus in winter, Siphonalia spadicea fuscolineata in spring, and Crangon hakodatei in summer. Dominant species in abundance were Chelidonichthys spinosus in autumn, Liphius litulon in winter and spring, and Raja kenojei in summer. The number of species and their abundance, the species composition, and the diversity indices fluctuated with seasons. The organisms were divided into 17 groups by seasonal variation using a self-organizing map.

늦반딧불이 Pyrocoelia rufa의 유전적 지역 특이성

  • Lee, Sang-Cheol;Bae, Jin-Sik;Kim, Ik-Su;Jin, Byeong-Rae;Kim, Jong-Gil;Kim, Geun-Yeong;Kim, Sam-Eun;Suzuki, Hirobumi;Lee, Sang-Mong;Son, Heung-Dae
    • Proceedings of the Korean Society of Applied Entomolohy Conference
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    • 한국응용곤충학회 2001년도 추계 합동 심포지엄 및 학술발표대회
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    • pp.99-99
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    • 2001
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국내 늦반딧불이(Pyrocoelia rufa)의 분포 및 생활사

  • Kim, Jong-Gil;Kim, Geun-Yeong;Choe, Yeong-Cheol;Choe, Ji-Yeong;Kim, Sam-Eun;Jin, Byeong-Rae;Lee, Sang-Mong;Lee, Jong-Eun;Lee, Gi-Yeol;Lee, Sang-Ho
    • Proceedings of the Korean Society of Applied Entomolohy Conference
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    • 한국응용곤충학회 2002년도 추계 합동 특강 및 학술발표대회
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    • pp.94-94
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    • 2002
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Analysis of Vegetation Characteristics of Close-to-nature Creek Improvement (자연형 소하천정비사업의 식생특성 분석)

  • Yeon, Gyu-Bang;Kang, Sang-Jun;Yun, Kang-Hun;Lee, Jong-Hyung
    • Proceedings of the Korea Water Resources Association Conference
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    • 한국수자원학회 2007년도 학술발표회 논문집
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    • pp.657-661
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    • 2007
  • 본 연구에서는 자연형 소하천정비 시범사업 중에서 2003년 하천정비공사가 완료된 후 식생의 침입과 정착하는 과정을 조사함으로서 교란된 하천을 자연형 하천으로 복원하는데 생태학적으로 어떤 공법이 최적의 공법인가를 알아보고자 각 호안공법 별로 식생의 변화특성을 분석한 것으로 결론은 다음과 같다. 이동 소하천에 적용된 다양한 공법(자연석 쌓기, 식생축조블록, 그린리버(환경)블록, 스톤넷, 친환경 돌망태, 식생매트, 환경블록 H형, 반딧불 블록, 지오그린셀, 자연석 3단쌓기 호안)으로 정비된 자연형 하천 복원 후의 식생조성과 목본식물의 침입 및 정착 과정을 조사하였다. 9가지의 공법 중에서 식생 조성과 목본식물의 침입과 정착에 양호하다고 판단된 공법은 자연석 2단 및 3단 쌓기가 가장 좋다고 판단되었고, 그 다음은 지오그린셀 공법, 식생 매트 공법 그리고 그린리버 공법의 순이었다. 친환경 돌망태 공법, 환경블록 공법, 환경 H블록 공법, 식생 축조 블록 공법 및 반딧불 블록 공법은 다른 공법에 비하여 적절하지 못한 공법이었다. 각 공법 간 식생 조성의 유사도 비교에 의하면 시간이 지남에 따라 하천 복원 후 하천 변의 식생이 한 방향으로 서서히 수렴되어 가고 있었다. 하천 공법에 관계없이 봄에 침입하는 식물을 보면 둑의 사면에는 갈퀴나물, 큰김의털, 쑥, 환삼덩굴, 점나도나물 이었고, 수로에는 고마리, 소리쟁이, 미나리, 개구리자리였다. 여름에는 둑 사면에 망초, 억새, 환삼덩굴, 토끼풀이, 수로에는 고마리, 미나리, 미국가막사리, 닭의장풀, 그리고 가을에 많이 침입한 식물은 사면에 파종된 외래식물인 큰김의털, 식재한 억새, 강아지풀 및 환삼덩굴이었음을 알 수 있었다. 처음 침입한 목본은 갯버들, 아카시나무, 내버들 및 버드나무 등 4종이었으나, 1년 후에는 싸리, 산딸기, 쥐똥나무 및 뽕나무가 새로 침입하였다. 이렇게 침입한 목본식물들은 복원된 하천에 다른 물리적인 교란이 심하게 가해지지 않는다면 당분간 계속 생장할 것으로 사료된다.

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Study on the Safety of Firefly Luciferase in Human as a Transient Reporter Gene of Oncolytic Virotherapy (항암 바이러스 치료제의 보고유전자로써 반딧불이 루시퍼레이즈의 인체 내 안전성에 대한 연구)

  • Hong, Young Mi;Yoon, Woong Hee;Lee, You Ra;Kim, Soo Ji;Ngabire, Daniel;Narayanasamy, Badrinath;Ornella, Mefotse Saha Cyrelle;Kim, Myunghee;Cho, Euna;Lee, Bora;Hwang, Tae-Ho
    • Journal of Life Science
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    • 제31권11호
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    • pp.1028-1036
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    • 2021
  • Firefly luciferase (FLuc) can function as an efficient marker in the gene and viral therapies. Nonetheless, its clinical translation has been unaccomplished with the concerns on its exogenous nature and the similarity with human fatty acyl-CoA synthetase. In this study, we aimed to show safety of FLuc by conducting a set of preclinical experiments and a human use. Initially, FLuc permeability across the plasma membrane was investigated by delivering the FLuc-carrying viral vector, OTS-412, or the FLuc recombinant protein. After in vitro infection of OTS-412 into different cancer cell lines, FLuc activity was detected only in the cell lysates, but not in culture media. In addition, recombinant FLuc protein further showed the impermeability against the plasma membrane. Similar result was also observed in the in vivo experiment. After being injected into the VX2 tumor-bearing rabbit, the FLuc exclusively resided within the tumor tissue without being detected in the blood plasma or other organs. Human cancer cell lines originated from various organs were lysed and treated to the FLuc, and none of the human substrates was reactive against the FLuc. As a final step, FLuc recombinant protein was intravenously injected into a human. The luciferase was degraded with the half-life of 20 to 30 minutes in blood, and was untraceable from 1.5 hr after the injection. In addition, the blood plasma was nonresponsive against the fatty acids. Hematological analysis was also comparable between the pre- and post-injection. Altogether, our study collectively demonstrates the safety of the firefly luciferase.

Effects of Temperature on Reproduction and Development of Firefly, Luciola lateralis(Coleoptera: Lampyridae) (온가가 애반딧불이의 생식과 발육에 미치는 영향)

  • 이기열;안기수;강효중;박성규;김종길
    • Korean journal of applied entomology
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    • 제42권3호
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    • pp.217-223
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    • 2003
  • Effects of temperature on the development and reproduction of the Luciola lateralis were investigated at various temperatures. The development time of eggs, larvae, and pupae were shorter at higher temperatures than at lower ones. The insect did not develop at 10$^{\circ}C$ and 35$^{\circ}C$. The hatchability was 61.5% at 15$^{\circ}C$, 73.9% at 20$^{\circ}C$, 93.3% at 23$^{\circ}C$, 91.8% at 25$^{\circ}C$, 74.0% at 27$^{\circ}C$, and 46.0% at 30$^{\circ}C$, indicating the best hatchability rate at the temperature condition of 23 DC. Larval periods were 341.5:t 23.2 days at 15$^{\circ}C$, 265.5${\pm}$17.5 days at 20$^{\circ}C$, and 250.9${\pm}$11.7 days at 25$^{\circ}C$. Pupal periods were 94.7${\pm}$11.5 days at 15$^{\circ}C$, 41.7${\pm}$9.1 days at 20$^{\circ}C$, and 18.5${\pm}$7A days at 25$^{\circ}C$. Emergence rate was 23.3, 89.3 and 80.7%, respectively at the above temperatures. Adult longevity of female was 18.0 days at 15$^{\circ}C$, 2004 days at 20$^{\circ}C$, 10.7 days at 25$^{\circ}C$, and 5.8 days at 30$^{\circ}C$. Mean fecundity per female was higher at 20$^{\circ}C$ compared with at other temperatures. The developmental zero point temperatures (1) and the total effect temperatures (I<) of egg, larva, pupa, and complete development were 10.6, 14.0, and l3.1$^{\circ}C$ and 214.8, 1,564.8, and 229.2 degree-days, respectively. Mean generation time in days (T) was shorter at higher temperature. Net reproductive rate per generation (Ra) was the lowest at the highest temperature as well as at the lowest, and it was 177.19 which was the highest at 23$^{\circ}C$. The intrinsic rate of natural increase (r$\sub$m/) was highest at 27$^{\circ}C$ as 0.019. As a result, optimum range of temperature for L. lateralis growth was between 20-25$^{\circ}C$.

Effect of Water temperature on the Climbing up of Larvae of Firefly, Luciola lateralis (Coleoptera: Lampyridae) (애반딧불이(Luciola lateralis) 유충의 상륙에 미치는 수온의 영향)

  • Oh, Hong-Sik;Kang, Young-Kook;Nam, Sang-Ho
    • Korean journal of applied entomology
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    • 제48권2호
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    • pp.203-209
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    • 2009
  • This study was conducted to investigate the relationship between the climbing up event of Luciola lateralis larvae and water temperature. In the laboratory condition, the larvae did not come out of water at constant temperature condition of 19.3${\pm}$$0.3^{\circ}C$ and tried to climb up at varying temperature condition of 20${\sim}$$22^{\circ}C$ (mean temperature 20.9${\pm}$$0.9^{\circ}C$) without success. However, they climbed up at constant temperature condition of 20.8${\pm}$$0.6^{\circ}C$. The frequency of the larval climbing up was highest as 63.6% at 20${\sim}$$21^{\circ}C$. The most larvae climbed up at approximately $21^{\circ}C$ of average daily water temperature, In the natural condition, the larvae climbed up at 19.8${\sim}$$21.7^{\circ}C$ and the frequency of the larval climbing up was high as 80.4% at 20${\sim}$$21^{\circ}C$. The larval climbing up was also little observed at 19${\sim}$$20^{\circ}C$ at which no larval climbing up was found in the laboratory experiment. No larvae attempted to climb up when water temperature was below $19^{\circ}C$ and above $22^{\circ}C$. It took 18 days from climbing up of larvae to appearance of adults. In its habitat, the range of water temperature required for larvae to climb up is assumed to be 19.6${\sim}$$21.8^{\circ}C$ and the most suitable water temperature may be 20.4${\pm}$$2.3^{\circ}C$. Therefore, the range of water temperature required for larvae to climb up is assumed to be 19.8${\sim}$$21.7^{\circ}C$, and the suitable water temperature range for larvae may be 20${\sim}$$21^{\circ}C$, and the most suitable temperature is thought to be $21^{\circ}C$.

Bionomical Characteristics of Luciola lateralis (Coleoptera: Lampyridae) in Mass Breeding (애반딧불이(딱정벌레목: 반딧불이과)의 생육 특성)

  • Kim, Ha-Gon;Kwon, Yong-Jung;Suh, Sang-Jae
    • Journal of Life Science
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    • 제18권12호
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    • pp.1728-1732
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    • 2008
  • This study was performed to investigate the bionomical characteristics of Luciola lateralis in Korea. Imago's longevity of female was $17.5{\pm}0.7$ and male was $20.6{\pm}1.0$ days at $23^{\circ}C$, R.H. 80%, and significant difference was not occurred in breeding density. Pre-oviposition period was a pair $2.8{\pm}1.5$, two pairs $2.5{\pm}1.5$, three pairs $1.7{\pm}0.7$, four pairs $1.3{\pm}0.5$ days and pre-oviposition period was shorten in higher breeding density. The mean of laying eggs was $248.9{\pm}80.7$, was not related breeding density. Daily oviposition rate was 1st day 20.8%, 2nd 17.3%, 3rd 14.3%, 4th 12.7%, 5th 7.0%, the rate was highest in 1st day and gradually reduced. Egg period was $21.8{\pm}0.7$ days and hatchability was $95.5{\pm}4.8%$. Larval instar was identified the 9th instar, the evidence was able to its skin and marking pattern. Larval period of each instar was 1st $1.0{\pm}0.1$, 2nd $3.3{\pm}0.2$, 3rd $10.2{\pm}1.0$, 4th $6.8{\pm}0.5$, 5th $6.9{\pm}2.0$, 6th $15.4{\pm}4.1$, 7th $18.3{\pm}5.9$, 8th $25.8{\pm}8.7$, 9th $31.2{\pm}13.2$ days. Matured 9th larva was made of cocoon during $2.8{\pm}0.8$ days and pre-pupal, pupal period was $4.6{\pm}0.9$, $5.6{\pm}0.7$ days. Imago was made hard hind wing during $3.8{\pm}0.4$ days in cocoon and come out.