• The Science & Technology
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• v.35 no.11 s.402
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• pp.70-71
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• 2002

• Proceedings of the KAIS Fall Conference
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• 2006.11a
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• pp.309-312
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• 2006

최근에는 산업의 발전에 따라 다종 다양한 형태의 중금속이 이용되고 있지만 처리공정의 효율상의 한계성 때문에 미량의 중금속이 배출되어 생태계의 치명적인 위협요소로 부각되고 있다. 중금속이 포함된 폐수는 일반적으로 여러 가지 방법이 있지만 생체물질을 이용한 생체흡착에 대한 연구 및 공정 개발이 활발히 이루어지고 있다. 생체흡착은 중금속이 생물체 표면이나 내부로 물리 화학 및 생물학적 상호작용에 의한 이온교환, 흡착등 다양한 기작에 의해 수용액으로부터 중금속이 제거되는 것이다. 본 연구에서 바실러스에 의한 EPS 물질을 추출하였으며 포자화 전과 후의 EPS를 이용하여 중금속제거 실험을 하였다. EPS 물질은 Protein이 Carbohydrate보다 많은 함량을 보였으며 중금속 제거는 포자화 전보다 포자화 후의 EPS가 더 많이 제거되는 것으로 나타났다.

• 농업기술회보
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• v.51 no.5
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• pp.17-18
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• 2014

최근 들어 식물의 뿌리 주변에는 사는 근권미생물과 식물과의 상호작용연구가 새롭게 조명되고 있다. 바실러스, 슈도모나스 등의 근권미생물은 식물의 면역기능을 활성화시켜 작물의 건강을 지키는 것으로 밝혀지고 있으며 이러한 기술을 도입할 경우 농작물의 안전 관리에 중요한 역할을 한다.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2006.11a
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• pp.357-361
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• 2006

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.5
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• pp.819-823
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• 2011

The present study evaluated the seasonal changes of the soil microbial population by selected media in an organic farming system (OFS) with rye rotation cropping management compared to those in a conventional farming system (CFS) with chemical fertilizers, pesticide and herbicide from May 2009 to October 2010 in an upland field. With the exception of fungi, populations of aerobic bacteria, Gram-negative bacteria, and Bacillus spp. were higher in the OFS soil during soybean-growing stages. In addition, populations of aerobic bacteria, Gram-negative bacteria, and Bacillus spp. in the OFS soil were nearly two times more than those in the CFS soil on reproductive growth stages. Our findings suggested that Bacillus spp. should be considered as responsible factor for microbial population differentiation observed between the OFS and the CFS in upland fields.

• Journal of the Korean Applied Science and Technology
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• v.37 no.6
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• pp.1535-1544
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• 2020

The primary objective of this study was to isolate and identify amine oxidase-producing probiotic Bacillus strains from traditional fermented soybean paste. Biogenic amines (BA)-forming bacteria isolated from the samples were identified as Bacillus sp. TS09, Bacillus licheniformis TS17, Bacillus subtilis TS19, Bacillus cereus TS23, Bacillus sp. TS30, Bacillus megaterium TS31, B. subtilis TS44, Bacillus coagulans TS46 and Bacillus amyloliquefaciens TS59. Meanwhile, B. subtilis TS04 and TS50 isolated from the same samples exhibited good probiotic properties, including the tolerance to artificial gastric juice and bile salts, the adhesion to intestinal epithelial cells, and the production of bacteriocin(s) active against BA-forming bacteria (Bacillus sp. TS30 and B. subtilis TS44). In addition, the amine oxidase produced by B. subtilis TS04 and TS50 significantly decreased the formation of BA, especially cadaverine, putrescine, and tyramine, therefore, these strains could be considered good potential probiotic candidates to prevent or reduce BA accumulation in food products.

• Journal of Life Science
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• v.28 no.7
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• pp.827-834
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• 2018

In this study, the biological activities of aqueous, ethanol, and methanol extracts of larvae of the edible insect Protaetia brevitarsis seulensis, fermented using several kinds of microorganisms, were tested in in vitro experimental models. Six effective microorganisms were used for fermentation, namely Lactobacillus plantarum JBMI F3, Lactobacillus plantarum JBMI F5, Lactobacillus gasseri Ba9, Aspergillus kawachii KCCM 32819, Saccharomyces cerevisiae KACC 93023, and Bacillus subtilis KACC 91157. Biological activities (${\alpha},{\alpha}^{\prime}-diphenyl-{\beta}-picrylhydrazyl$ [DPPH] free radical scavenging activity, reducing power, and fibrinolytic activity), and biochemical properties (phenolic compounds and flavonoids) were examined in aqueous, ethanol, and methanol extracts from P. brevitarsis seulensis powder and fermented P. brevitarsis seulensis powder. The total phenolic compounds and flavonoid contents were highest in the aqueous extract of B. subtilis-fermented P. brevitarsis seulensis powder. DPPH radical scavenging activity and reducing power were stronger in the fermented group than the nonfermented group. Fibrinolytic activity were highest in the extract from B. subtilis-fermented P. brevitarsis seulensis powder. The ${\alpha}-amylase$ activity in starch was higher in the fermented group than the nonfermented group, but there was no significant difference. These results provide basic data to understand the biological activities of bioactive materials derived from fermented P. brevitarsis seulensis larvae for the development of functional foods.

• Microbiology and Biotechnology Letters
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• v.47 no.4
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• pp.596-602
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• 2019

In September 2017, the rhizospheric soil of Tetragonia tetragonoides (Pall.) Kuntze was further sampled. One hundred and thirty eight species of microorganisms were isolated from the soil. Indole-3-acetic acid (IAA) production, siderophore production, and phosphate degradation were examined in order to confirm bacterial growth from isolated microorganisms. As a result, most strains were able to produce auxins or siderophores and to solubilize phosphate. In addition, 138 isolated strains were treated with tobacco extract and conferred pathogen resistance to host plants upon treatment. As a result, 35 strains that were able to reduce pathophysiology by more the 60% were selected. Among them, 6 strains with high induced systemic resistance (ISR) activity were found. All of these strains belong to the genus Bacillus according to the 16S rDNA sequence analysis. Bacillus aryabhattai KUDC6619 showed outstanding effects with reduced infection in tobacco and pepper plants. Probably, these Bacillus species play a beneficial role by association with T. tetragonoides for its survival in the harsh conditions found on the island of Dokdo.

• Journal of Marine Bioscience and Biotechnology
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• v.2 no.1
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• pp.48-54
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• 2007

Bacillus polyfermenticus SCD (B. polyfermenticus SCD) has been appropriately used for the therapy of long-term intestinal disorders, because live strains in the form of active endospores can successfully reach the target intestine in humans. B. polyfermenticus SCD produces the most antibiotic-related materials. In the present study, B. polyfermenticus SCD was fermented with soybeans (BPFS) and its probiotic properties were investigated. B. polyfermenticus SCD and BPFS showed a broad spectrum of antimicrobial activity against pathogenic Gram-positive (Streptococcus parauberis, Streptococcus iniae, Lactococcus garviae) and Gram-negative (Flexibacter tractuosus, Vibrio harveyi, Vibrio vulnificus, Vibrio ordalii) bacteria and moulds (Aspergillus niger, Aspergillus oryzae). Sebastes schlegeli were used to examine survival rate and cleanup action by BPFS. Bacterial infection resulted in a mortality of up to 99% in the commercial fodder fed groups. BPFS both enhanced the growth rate of fry by improving their appetite and had cleaned by decreased eutrophication. Therefore, it seems appropriate that BPFS should be developed as an antibiotic replacement, favorable fodder additive, and antifungal material in fish farming systems.

• The Korean Journal of Pesticide Science
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• v.15 no.3
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• pp.323-328
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• 2011

Control efficacies of mixing of powdery mildew biofungicides with other control agents against major or fungal diseases of cucumber were investigated. Control efficacies against cucumber powdery mildew were quite different according to the kinds of biofungicides applied but those of powdery mildew biofungicides were increased by mixing application of two biofungicides. More than 80% of control efficacies on powdery and downy mildews of cucumber were obtained by mixing application of a powdery mildew biofungicide Bacillus subtilis KB-401 and a downy mildew chemical fungicide dimethomorph. Similarly, control efficacies on powdery and downy mildews of cucumber were 95% and 70% by mixing application of a powdery mildew biofungicide Bacillus subtilis KB-401 and cooking oils and yolk mixture, respectively.