• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.428-434
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• 2013

An isolate of Bacillus amyloliquefaciens subsp. plantarum CC110 was selected as an effective antagonist for biological control of cucumber downy mildew caused by Pseudoperonospora cubensis. Temperature range for growth of CC110 isolate was $7.5{\sim}55.0^{\circ}C$, and its optimal temperature at $36.6^{\circ}C$. pH range for growth of CC110 isolate was 4.5~9.5, and its optimal pH at 7.0. In this study, the most effective sources of carbon and nitrogen for growth of CC110 isolate were fructose and yeast extract, respectively. The volatile of CC110 isolate was found to be effective to control downy mildew on cucumber showing no diseased area whereas that of control was 13.2% using the I plate bioassay. The culture broth and cells of isolate CC110 cultured in TSB media for 48 hours at $28^{\circ}C$ inhibited occurrence of cucumber downy mildew. The cells and culture broth were transformed into sporangia of P. cubensis by in observation under light microscope and scanning electron microscope.