• Journal of Animal Science and Technology
• /
• v.45 no.5
• /
• pp.777-786
• /
• 2003

An experiment was conducted to investigate the effects of supplementary Blended essential oil(CRINA$^{\circledR}$) on the performance, nutrient availability, fatty acid composition of leg muscle, small intestinal microflora and blood parameters in broiler chickens. One thousand unsexed day-old broiler chickens were assigned to five treatments : control(T1), 5ppm avilamycin(starter diet) & 5ppm flavomycin(grower diet) T2, 5ppm avilamycin(starter diet) & 50ppm CRINA$^{\circledR}$(grower diet) T3, 50ppm CRINA$^{\circledR}$(starter & grower diet) T4, 50ppm CRINA$^{\circledR}$+ 500ppm lactic acid$^{\circledR}$ (starter & grower diet) T5. Each treatment had four replications of 50 birds each. Growth performance was significantly improved by dietary supplements(T2-T5). There were no significant differences among treatment T2, T3, T4 and T5. Feed intake was not significantly different among treatments. Dietary supplementation of CRINA$^{\circledR}$(T3, T4, T5) resulted in significant(p〈0.05) improvement in feed/gain(F/G) during finishing period (4-5weeks). The birds fed CRINA$^{\circledR}$ supplemented diet(T4) showed significantly(p〈0.05) higher availability of crude fat, methionine and methionine + cystine than those fed antibiotics supplemented diet(T2). Mortality was not significantly affected by treatments. The colony forming unit(CFU) of E.coli in small intestinal content was significantly lower in antibiotics & CRINA$^{\circledR}$(T3) compared to CRINA$^{\circledR}$ treatment(T4)(P〈0.05). CFU of Cl. perfringens was low in CRINA$^{\circledR}$(T4) but not different significantly with other treatments. Serum triglyceride level of birds fed CRINA$^{\circledR}$ + lactic acid diet(T5) was significantly lower(p〈0.05) than those fed antibiotics supplemented diet(T2). Cholesterol level of the birds fed antibiotics(T2) or CRINA$^{\circledR}$ + lactic acid supplemented diet(T5) was significantly higher(p〈0.05) than other treatments. HDL level of birds fed control diet was significantly lower(p〈0.05) than that of others. The levels of serum IgG were not significantly different among treatments. Major fatty acids composition of leg muscle fat was significantly influenced by treatments. Control group showed significantly higher palmitic acid(C$_{16:0}$) and steraric acid(C$_{18:0}$) content than other treatments(p〈0.05). Content of oleic acid(C$_{18:1}$), however, was significantly lower in the control than others treatments. Content of linolenic acid(C$_{18:3}$) was significantly higher in CRINA$^{\circledR}$+ lactic acid(T5) than antibiotics & CRINA$^{\circledR}$(T3) treatments. Total saturated fatty acids content was higher and total unsaturated fatty acids were lower in the leg muscle fat of the control than that of other treatments. It is concluded that CRINA$^{\circledR}$ supplementation improved growth rate and F/G ratio in broilers. The combination of CRINA$^{\circledR}$ with either antibiotics or lactic acid did not show any additive or synergistic effects in broiler chickens .

• Korean Journal of Microbiology
• /
• v.41 no.3
• /
• pp.183-187
• /
• 2005

This study was performed to investigate the hygiene condition of PC room(internet cafe) in Seoul Korea. From July 2004 to December, 34 samples were collected, there's an average of $9.0{\times}10^4$ CFU/ml on keyboards, $2{\times}10$ CFU/ml on mouse and $5{\times}10^3$ CFU/ml on door konbs toilets, suggesting that keyboards and mouse are more contaminated than toilet door knobs. Seven antimicrobial resistant strains were isolated from PC Rooms. Two isolates were resistant to methicillin and erythromycin, while five isolates were resistant to gentamicin, ampicllin, cefotaxim, and chloramphenicol. By identification, these strains were identified as Staphylococcus aureus (2 strains). Actinobacillus ureae (4 strains) and Pasteurella multocida (1 strain), respectively. Pasteurella multocida and Actinobacillus ureae are potentially pathogenic bacteria. Actinobacillus ureae, formerly, known as Pasteurella ureae, is an uncommon of the upper respiratory tract in humans. Pasteurella multocida is a part of the normal flora in the nasopharynx of many domestic animals. We concluded that Staphylococcus aureus is highly resistant to erythromycin and methicillin over $100\;{\mu}g/ml$, while Pasteurella multocida and Actinobacillus ureae is highly resistant to gentamicin, ampicillinover over $100\;{\mu}g/ml$.

• Microbiology and Biotechnology Letters
• /
• v.44 no.4
• /
• pp.504-511
• /
• 2016

Lactulose, a synthetic disaccharide, has received increasing interest because of its role as a prebiotic that can increase the proliferation of Bifidobacterium and Lactobacillus spp. and enhance the absorption of calcium and magnesium. While the industrial production of lactulose is still mainly achieved by the chemical isomerization of lactose in alkaline media, this process has drawbacks including the need to remove catalysts and by-products, as well as high energy requirements. Recently, the use of cellobiose 2-epimerase (CE) has been considered an interesting alternative for industrial lactulose production. In this study, to develop a process for enzymatic lactulose production using CE, we screened improved mutant enzymes ($CS-H^RC^E$) from a library generated by an error-prone PCR technique. The thermostability of one mutant was enhanced, conferring stability up to $75^{\circ}C$, and its lactulose conversion yield was increased by 1.3-fold compared with that of wild-type CE. Using a recombinant Escherichia coli strain harboring a CS35 $H^RC^E$-expressing plasmid, we prepared cell beads immobilized on a Ca-alginate substrate and optimized their reaction conditions. In a batch reaction with 200 g/l lactose solution and the immobilized cell beads, lactose was converted into lactulose with a conversion yield of 43% in 2 h. In a repeated 38-plex batch reaction, the immobilized cell beads were relatively stable, and 80% of the original enzyme activity was retained after 4 cycles. In conclusion, we developed a reasonable method for lactulose production by immobilizing cells expressing thermostable CE. Further development is required to apply this approach at an industrial scale.

• Microbiology and Biotechnology Letters
• /
• v.34 no.1
• /
• pp.28-34
• /
• 2006

A native extracellular acid phosphatase, phytase (EC 3.1.3.8), from Bacillus coagulans IDCC 1201 (commercially known as Lactobacillus sporogenes) used as probiotics, was characterized. Though some strains of B. coagulans have been evaluated with regard to several health-promoting effects, it has not been reported to produce phytase. Partially purified phytase front the strain IDCC 1201 had a pH optimum of 4.0 and a temperature optimum of $50^{\circ}C$, respectively. The requirement for divalent cations was studied and cobalt ion remarkably increased the enzyme activity. The removal of metal ions from the enzyme by EDTA decreased activity below 50%. The enzyme activity depleted restored when the assay was performed in the presence of $Co^{2+}$. Also, $Co^{2+}$ is the most active stimulator and has unique activation effect at high temperature. The phytase was specific for sodium phytate and p-nitrophenylphosphate, which is different from other known Bacilli phytases. The putative amino acid sequences of the phytase from B. coagulans IDCC 1201 were very similar to that of the phytase from B. subtilis strain 168. Based on these data, we concluded that the phytase from B. coagulans IDCC 1201 is a $Co^{2+}$-dependent acid phosphatase. Therefore, the strain B. coagulans IDCC 1201 is thought to be a valuable addititive for livestocks as well as a beneficial probiotics for human.

• Microbiology and Biotechnology Letters
• /
• v.30 no.4
• /
• pp.359-366
• /
• 2002

Bacillus polyfermenticus SCD which is commonly called as Bisroot strain is being used for functional foods through the treatment of long-term intestinal disorders, since the live strains in the form of active endospores can successfully reach the target intestine in humans. The cells of B. polyfermenticus SCD were treated for 4h in artificial gastric juice (pH 2.0,3.0) and bile acid. Final viability of the strain in artificial gastric Juice (pH 2.0, 3.0) is reached to 62.8% and 81.2% respectively B. polyfermenticus SCD is resistant to antibiotics such as streptomycin, rifampicin, nystatin and ampicilin. B. polyfermenticus SCD is well known supplies the nutrients by synthesizing vitamin $B_1$, $B_2$, C and K. B. polyfermenticus SCD produces various digestive enzymes and the enzymes enable to completely digest diets in our body. Above all, $\alpha$-amylase and pretense activities are very higher than B. subtilis KCTC 1020, about two fold and twenty five fold respectively. B. polyfermenticus SCD is very stable during long-term storage period in phosphate buffers of wide-range pH, solutions of various concentrations of sodium chloride, 5% glucose solution and water.

• Journal of Food Hygiene and Safety
• /
• v.31 no.3
• /
• pp.141-152
• /
• 2016

The demand to develop more safe and efficient methods for treating allergic patient is now continuously growing due to the increasing prevalence of allergic diseases. Probiotics are endogenous microbial flora that gives health benefits within hosts. Probiotics are now considered as one of solutions to treat allergic patients since recent evidence shows that some of probiotics have immunomodulatory function. Also, the treatment of probiotics to patients is relatively safer than other anti-inflammatory agents. In this review, we summarized on immunomodulatory function of some probiotics which show preventive or therapeutic effects on major allergic diseases such as atopic dermatitis, allergic rhinitis, asthma, or food allergy. Based on previous literature, the treatment of probiotics can alleviate the symptoms of allergic diseases via balancing $Th_1/Th_2$ response or increasing the number of regulatory T ($T_{reg}$) cells.

• Journal of Life Science
• /
• v.24 no.7
• /
• pp.784-790
• /
• 2014

Housefly (Musca domestica L.) maggots are used as biomedical material. Ethanolic extracts of fly maggot (EM) were orally administered to male rats at levels of 0 (control group), 4.0, 6.0, and 8.0 mg per 100 g live weight for 40 days. Serum triglycerides, total cholesterol, and LDL-C decreased by 17.90, 17.60, and 16.37%, respectively, whereas HDL-C increased by 20.48% in the EM group compared with these parameters in a control group (p<0.05). Thymus and spleen weights dose-dependently increased by 21.42% and 21.42%, respectively, but abdominal fat decreased by 39.66% after EM administration compared with that in the control group (p<0.05). IgG, IgA, and IgM increased 35.14, 68.65, and 190.16%, respectively, in the EM groups compared to the control group (p<0.05). Bifidobacterium and Lactobacillus increased by 41.68% and 35.55%, respectively, in the EM groups compared with the control group, and Bacteroides, Clostridium, Escherichia, and Streptococcus decreased by 24.96, 46.37, 25.00, and 34.05%, respectively, in the EM groups compared with the control group (p<0.05). Compared with the control group, total organic acids, acetic acid, and propionic acid increased by 31.11, 49.34, and 24.88%, whereas butyric acid, isobutyric acid, valeric acid, and isovaleric acid decreased by 30.79, 72.64, 32.90, and 63.16% respectively, in the EM groups (p<0.05). These results suggest that EM has a bifidogenic effect on immune cell development, blood lipid levels, and abdominal fat reduction by increasing the production of organic acid and numbers of cecal microorganisms in animals.

• Korean Journal of Poultry Science
• /
• v.34 no.4
• /
• pp.287-294
• /
• 2007

Current study was conducted to identify the effects of dietary supplementation of Aspergillus oryzae ferments (AOF) cultured under normal (NAOF) or nitrogen-deficient (NMAOF) environment on feed efficiency, nutrient digestibility for broiler chicks. Fecal microbes and ammonia gas production were also determined. A total of 168 male Avian chicks, 2-wk-old, were randomly assigned into 56 cages, three chicks per cage. There were seven treatments (Control, NAOF 0.05, 0.1, 0.5%, NMAOF 0.05, 0.1, 0.5%), with 8 replicates (cages) per treatment. There was no significant difference in nutrient digestibility between two AOF groups, but the digestibility was greatly(p<0.05) improved by AOF supplementation. Total microbial account significantly (p<0.05) differed between the treatment groups with the highest number for NNAOF, followed by NAOF and control. In the case of Escherichia coli and Salmonella, the AOF supplementation significantly (p<0.05) reduced their numbers in feces, with a particular reduction in NNAOF group. Levels of ammonia gas generation were in order of control>NAOP>NNAOP. The current data implied that AOF supplementation, particularly grown under nitrogen-deficient environment, would be a feasible way to improve feed efficiency for broiler production, as well as to reduce environmental cost. However, further studies remain for industrial application.

• Microbiology and Biotechnology Letters
• /
• v.26 no.3
• /
• pp.221-225
• /
• 1998

Fourteen healthy volunteers ranged in ages from 20 to 30 were served to administrate two types of yoghurt (2 bottles/day) such as one added uncapsulated-Bifidobacteria (Y-UCB) and the other added capsulated-Bifdobacteria (Y-CB) for 4 weeks, and the changes of intestinal microflora and fecal properties were studied. After administration of Y-UCB, the viable cell counts of fecal Bifidobacteria (p<0.01) and Lactobacilli (p<0.05) were significantly increased, however, fecal pH, moisture content and tile viable cell counts of coliform bacteria in feces were not changed when they were compared to those before administration (control). After administration of Y-CB, the viable cell counts of Bifidobacteria were significantly increased (p<0.01) and viable cell counts of coliform bacteria were significantly decreased (p<0.05), however, fecal pH, moisture content, and viable cell counts of Lactobacilli were not changed when they were compared to those before administration. High level of fecal Bifidobacteria and low pH were maintained after 2 weeks from ceasing the administration of both types of yoghurt when they were compared to those before administration. In conclusion, there were not significant differences between two types, Y-CB and Y-UCB in the changes of fecal pH, moisture content, and the viable cell counts of Bifidobacteria, Lactobacilli, coliform bacteria after the administration.

• Korean Journal of Poultry Science
• /
• v.50 no.3
• /
• pp.171-185
• /
• 2023

This study was conducted to investigate the effects of supplementation of Allium hookeri (AH) root powder on the gut microbiome, immunity, and health in broiler chickens fed experimental diets from d 10 to 28. A total of 60 10-day-old Ross 308 broilers were weighed and assigned to two dietary treatments with 5 birds per cage in a randomized complete block design based on body weight. The two experimental diets consisted of a control diet based on corn-soybean meal and the control diet supplemented with 0.3% AH root powder. All birds were fed ad libitum with experimental diets and water for 18 d. At 28 d, two birds near the median weight from each cage were selected for cecal content and small intestinal tissue sample collection. The addition of AH changed the gut microbiome by increasing probiotic candidate beneficial bacteria such as Enterococcaceae, Lactobacillaceae, Limosilactobacillus, Cuneatibacter, and Ruminoccoides. Regarding gut immunity, the supplementation of AH resulted in changes in intestinal immune cells, including reduced CD3+CD4+ T cells, which are a type of helper T cell, in the small intestine of birds (P=0.049). Additionally, there was a tendency to increase the expression of antioxidant function-related gene such as GPX2 (P=0.060), but no significant changes were observed in cytokines such as IL1b, IL6, and IL10. Overall, the addition of AH root powder may have positive effects on the microbiome of the chickens. This may help promote gut health in broiler chickens at the age of d 10 to 28.