• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.209-209
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• 2011

본 연구는 횡성다목적댐이 준공되면서 유수역이었던 섬강이 정수역의 환경으로 변하면서 나타난 어류의 군집변화에 대하여 알아보고자 시행하였다. 조사 지역인 횡성다목적댐은 강원도 횡성군 갑천면 대관대리에 위치하고 있으며, 높이 48.5m, 길이 205m, 총저수량 8,690만t의 다목적댐으로 1993년 12월에 착공하여 2000년 11월에 준공되었다. 어류 조사는 2010년 04월부터 2010년 11월까지 총 4회에 걸쳐 횡성다목적댐을 기점으로 상류 2지점(유평교, 매일교), 횡성호 2지점(구방교, 부동교), 하류 2지점(활아지교, 수백교)에서 조사를 실시하였고 과거 문헌과 비교하여 결과를 분석하였다. 횡성다목적댐의 착공 전/후 조사 결과를 비교한 결과 착공하기 전에는 총 8과 29종 2,325개체가 조사되었으며, 참갈겨니(Zacco koreanus) 576개체(24.8%), 쉬리(Coreoleuciscus splendidus) 316개체(13.6%), 피라미(Zacco platypus) 203개체(8.7%), 배가사리(Microphysogobio longidorsalis) 194개체(8.3%) 등의 순으로 기록되었다. 반면, 착공한 후에는 총 8과 24종 2,769개체가 조사되었으며, 피라미(Z. platypus) 2,169개체(78.3%), 밀어(Rhinogobius brunneus) 91개체(3.3%), 참갈겨니(Z. koreanus) 89개체 (3.2%), 돌고기(Pungtungia herzi) 83개체(3.0%) 등의 순으로 조사되었다. 각 조사지점별로 비교한 결과 상류 2지점(유평교, 매일교)에서는 착공하기 전에 총 6과 20종 609개체가 조사되었으며, 참갈겨니(Z. koreanus) 227개체(37.3%), 피라미(Z. platypus) 74개체(12.2%), 쉬리(C. splendidus) 65개체(10.7%), 돌고기(P. herzi) 43개체(7.1%), 납자루(Acheilognathus lanceolatus) 41개체(6.7%) 등의 순으로 기록되었다. 반면, 착공한 후에는 총 6과 16종 1,923개체가 조사되었으며, 피라미(Z. platypus)가 1,707개체(88.8%)로 대부분을 차지하였고, 그 외 참갈겨니(Z. koreanus) 53개체(2.8%), 돌고기(P. herzi) 46개체(2.4%), 참마자(Hemibarbus longirostris) 35개체(1.8%), 모래무지(Pseudogobio esocinus) 23개체(1.2%) 등의 순으로 조사되었다. 횡성호 2지점(구방교, 부동교)에서는 착공하기 전에 총 6과 22종 721개체가 조사되었으며, 참갈겨니(Z. koreanus) 177개체 (24.5%), 배가사리(M. longidorsalis) 91개체(12.6%), 쉬리(C. splendidus) 88개체(12.2%), 묵납자루(A. signifer) 71개체(9.8%), 피라미(Z. platypus) 45개체(6.2%) 등의 순으로 기록되었다. 반면, 착공한 후에는 총 6과 12종 393개체가 조사되었으며, 피라미(Z. platypus) 228개체(58.0%), 붕어(C. auratus) 56개체(14.2%), 밀어(R. brunneus) 46개체(11.7%), 쏘가리(Siniperca scherzeti) 20개체(5.1%), 동자개(Pseudobagrus fulvidraco) 16개체(4.1%) 등의 순으로 조사되었다. 하류 2지점(활아지교, 수백교)에서는 착공하기 전에 총 8과 25종 995개체가 조사되었으며, 참갈겨니(Z. koreanus) 172개체(17.3%), 쉬리(C. splendidus) 163개체(16.4%), 묵납자루(A. signifer) 96개체(9.6%), 배가사리(M. longidorsalis) 91개체(9.1%), 피라미(Z. platypus) 84개체(8.4%) 등의 순으로 기록되었다. 반면, 착공한 후에는 총 7과 19종 453개체가 조사되었으며, 피라미(Z. platypus) 234개체(51.7%), 밀어(R. brunneus) 42개체(9.3%), 돌고기(Pungtungia herzi) 36개체(7.9%), 참갈겨니(Z. koreanus) 36개체(7.9%), 쉬리(C. splendidus) 10개체(4.2%) 등의 순으로 조사되었다. 본 연구의 조사 및 비교분석 결과 유수역이었던 섬강에 횡성다목적댐이 건설되어 주변 환경이 크게 변화하였고 그 결과 각 조사 지점에서의 어류상이 유수역 선호 어종에서 오염 내성종 및 정수역 선호 어종으로 변화되는 것을 확인하였다.

• Korean Journal of Environmental Biology
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• v.36 no.3
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• pp.352-358
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• 2018

This study aimed to develop a species identification method for the egg and fry of the three Korean bitterling fishes (Pisces: Acheilognathinae), including Acheilognathus signifer, Acheilognathus yamatsutae and Rhodeus uyekii based on the PCR-based Restriction Fragment Length Polymorphism (RFLP) markers. We conducted a field survey on the Deokchicheon River from the North Han River basin, where the three Acheilognathinae species co-occur, and also analyzed the existing sequence dataset available from the GenBank. We found coexistence of the three species at the study site. The egg and fry were obtained from the host mussels (Unio douglasiae sinuolatus) by hand from May to June 2015 and in May 2017. To develop PCR-based RFLP markers for species identification of the three Acheilognathinae fish species, restriction enzymes pinpointing species-specific single nucleotide variation (SNV) sites in mitochondrial DNA COI (cytochrome oxidase I) and cyt b (cytochrome b) genes were determined. Genomic DNA was extracted from the egg and fry and RFLP experiments were carried out using restriction enzymes Apal I, Stu I and EcoR V for A. signifer, A. yamatsutae and R. uyekii, respectively. Consequently, unambiguous discrimination of the three species was possible, as could be seen in DNA band patterns from gel electrophoresis. Our developed PCR-based RFLP markers will be useful for the determination of the three species for the young and would assist in studying the spawning patterns and reproductive ecology of Acheilognathinae fishes. Furthermore, we believe the obtained information will be of importance for future maintenance, management and conservation of these natural and endangered species.

• Korean Journal of Environmental Biology
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• v.42 no.1
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• pp.15-27
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• 2024

The goal of this study was to provide basic data necessary for managing fish in the Seomgang River by confirming diversity of fish species in the Seomgang River and determining the degree of change in fish species through a comparison with earlier related work. Growth rate, obesity level, and temporal and spatial reproductive capacities were analyzed to determine growth characteristics of dominant species. Fish fauna and structure of the fish community were surveyed at three different time points from October of 2020 to August of 2021 at eight sites. In addition, growth characteristics of Zacco platypus from June of 2021 to July of 2022 at four sites were determined. A total of 3,999 individuals, 43 species, and ten families of fish were identified. Among these species, 19 (44.19%) were Korean endemic species and four (9.30%) were endangered species. The dominant species was Z. platypus. Pungtungia herzi was identified as a subdominant species. An analysis of length-weight relationships and condition factor (k) of Z. platypus confirmed a good state of growth. As a result of a frequency analysis of the total length, the life cycle of the Z. platypus population living in the Seomgang River was found to be stable. According to gonadosomatic index (GSI) analysis, the spawning season of Z. platypus was estimated to occur after May, consistent with the literature.

• Parasites, Hosts and Diseases
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• v.31 no.4
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• pp.375-378
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• 1993

The prevalence of Intestinal parasite Infections In Inhabitants at the reaches of the Hantan River, Chorwon-gun, Korea, was observed from August 12 to September 14, 1993. Of 465 people observed by cellophane thick smear and formalin-ether concentration method, 2 Ascots lumbricoides (unfertilized), 1 Trichwis Dichturn, 39 Clonorchis sinensis and 16 Metagonimus egg positive cases were found. After treatment, the Metogonimus egg positive cases passed out flukes of Metogonimus Mlyata type. Of 68 fish caught In the Hantan River, 14 (20.6%) were Infected with metacercanae of Metogontmn, while no metacercana of C. sinenis was found. At this area, soul-transmitted nematodes are very low, but clonorchlasis and metagonlmlasis are prevalent by modernate endemicity.

• The Korean Journal of Ecology
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• v.28 no.5
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• pp.281-286
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• 2005

The ripe unfertilized eggs of Acheilognathus signifer were elliptical shape and baggily swollen toward animal pole. The size of fertilized eggs were $2.19{\pm}0.01$ mm in long axis and $1.85{\pm}0.08$ mm in short axis and oil globe were not observed. The zona radiata of A. signifer had the structure of regular fibrilar net and the diameter of outer opening micropyle was about $2.5{\mu}m$ and that of inner opening micropyle was about $1.5\sim2.0{\mu}m$. Development of fertilized eggs in A. signifer was proceeded in inclining position of animal pole and hatched in 49 hours under $20{\pm}1^{\circ}C$. The yolk sac was absolutely absorbed in 21 days after hatching.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.1
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• pp.39-44
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• 2014

This study aimed to find an optimal diluent and cryoprotective agent (CPA) during cryopreservation of common Korean bitterling Acheilognathus signifier sperm. The bitterling is an endangered species in Korea, and this study will enable conservation and further technical development of artificial seed production. We tested the effects of cryopreservation and toxicity by the type of diluent and CPA. The optimal combination of diluent and CPA for cryopreservation was 300 mM glucose+10% methanol, resulting in a survival rate and sperm activity index (SAI) of $96.3{\pm}1.5%$, and $3.0{\pm}0.0$ respectively, and no significant difference compared to fresh sperm. The survival rate and SAI of post-thawed sperm was $9.7{\pm}1.5%$, $0.8{\pm}0.3$ respectively, which was significantly higher than had been achieved with other diluents and CPAs.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.6
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• pp.1220-1225
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• 1999

Differences in carotenoid composition in the integument of Korean bittering, Cheilognathus signifer and bride bittering, Rhodeus ukekii which are Korean native fresh water fish were compared. Total ca rotenoid contents in the integument of wild Korean bittering was 2.11mg% and composed of 42.6% zeaxanthin, 12.1% diatoxanthin and 12.1% lutein epoxide which are predominant carotenoids and 10.3% cynthiaxanthin, 8.3% zeaxanthin epoxide, 6.4% lutein and 1.5% cryptoxanthin which are minor carotenoids. Total ca rotenoid contents in the integument of wild bride bittering was 4.99mg% during a spawning period but after the spawning period it was decreased to 4.17mg% and carotenoid composition of bride bittering during the spawning period was 46.7% zeaxanthin, 26.5% diatoxanthin and 12.3% lutein which are predominant carotenoids, and 6.2% zeaxanthin epoxide, 3.1% cynthiaxanthin, 2.9% cryptoxanthin and 0.7% canthax anthin which are minor carotenoids. These results indicated that the carotenoid composition of bride bittering during spawning period was very similar to that of Korean bittering and carotenoid composition of bride bittering after the spawning period was 30.5% diatoxanthin, 21.5% cynthiaxanthin and 16.8% zeaxanthin which are predominant carotenoids and 14.0% cryptoxanthin, 11.3% lutein and 3.4% can thaxanthin which are minor carotenoids, indicating that after the spawning period, the content of zeaxanthin was decreased while that of cryptoxanthin and cynthiaxanthin was increased as compared to that of the spawning period. Total carotenoid contents in Korean bittering and bride bittering was relatively higher than that in other species of cyprinidae whereas composition of the carotenoid was similar.

• Korean Journal of Ichthyology
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• v.17 no.2
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• pp.105-111
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• 2005

Most of the eggs and (or) pre-larvae of Acheilognathus signifer were observed from the gills of Unio douglasiae sinuolatus, 30~45 mm in the shell length, that is the host mussel of A. signifer. There was no selectivity in proportion to mussel size at the range observed. One to seven individual eggs and (or) pre-larvae were found in the mussels, with a mean of 2.5 individuals, and the rate of possessing one egg and (or) pre-larva was 50.0%. When the prelarva of A. signifer acquired swimming ability inside the mussel, it moved into the suprabranchial chamber. It was estimated that the growth period was 4~6 weeks. The minute tubercles of the pre-larvae were observed immediately after hatching. Absorption of the minute tubercles was observed starting the 7 th day, with most of tubercles absorbed 13 days after hatching and completed 20 days after the yolk was entirely absorbed.

• Korean Journal of Ichthyology
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• v.18 no.2
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• pp.141-147
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• 2006

The matured female and male of A. signifer differed in external morphology and body color. There were significant differences in PAD, PAB, PMP and PQR between matured female and male. Green color was very widely distributed in whole as nuptial color, that is similar to green and cyan color in the body part, yellow color in the ventral part and red and yellow color in the tail part. The nuptial organ of A. signifer was formed in a cluster on the horny substance of both sides above the mouth. There were 25~30 nuptial organs in a side. The nuptial organs disappeared with crumbling down from middle part of nuptial organ after spawning period. The nuptial organs disappeared mostly in September as non spawning period and a part of the nuptial organs remained as a vestige in the edges of both sides.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.547-547
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• 2015

본 연구에서는 달천구간 (괴산댐~송동보)에서 어류 군집 (Fish Community)을 고려한 생태학적 최적유량 (Ecological Optimum Discharge)을 산정하였다. 이를 위해 유량증진방법론(IFIM: Instream Flow Incremental Methodology)을 기반으로 한 2차원 모형인 River-2D를 적용하여 수리해석 및 대상어종 (Target Fishes)에 대한 가중가용면적 (WUA: Weighted Usable Area)을 산정하였다. 모형 검증을 위해 김원 등 (2007)에 의해 수행된 과업구간 내 수위 및 유량 모니터링 자료를 활용하였으며, 하류경계조건은 김지성 등 (2007)에 의한 수위-유량 관계곡선식을 활용하였다. 또한 평저류량 조건에서의 조도높이 (Roughness Height) 산정을 위해 유량 및 하상재료의 영향을 고려할 수 있는 멱함수 및 반대수함수 형태의 조도계수 공식을 적용하였다. 모형검증 결과 River-2D에 의해 계산된 수위값이 모니터링값과 비교적 잘 일치함을 알 수 있었다. 대상유역에 대한 어류 모니터링 (에코리버21사업단, 2007~2010) 결과를 바탕으로, 과업지역내 우점종 (피라미), 아우점종 (쉬리 등 3개 어종), 멸종위기어종 (묵납자루)을 대상어종으로 선정하였으며, 대상어종에 대한 서식도적합도지수 (HSI, Habitat Suitability Index) 산정을 위해 IFASG (Instream Flow and Aquatic Systems Group) 방법 및 WDWF (Washington Department of Fish and Wildlife) 방법을 적용하였다. 수심, 유속, 하상재료 및 하상형상에 대한 서식도적합도지수가 5가지 대상어종에 대해 각각 산정되었으며, 복합적합도지수 (CSI : Combined Suitability Index)를 고려한 과업대상 위치별 어류의 발생확률이 모의되었다. 어류 군집에 대한 가중가용면적 (WUA)이 최대로 되는 생태학적 최적유량 산정 결과, Type I~III의 경우 모두 $10m^3/s$ 이하의 유량조건에 대해서 WUA가 최대값을 갖는 것을 확인할 수 있었으며, 납자루속의 어류 (Type III)의 WUA가 000 및 000속 (Type II)의 어류에 비해서 작은 유량에서 더 큰 값을 갖으며, $10m^3/$s 초과 유량에 대해서는 반대의 경향이 나타나는 것을 확인할 수 있었다.