• Journal of Korean Society of Forest Science
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• v.71 no.1
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• pp.66-73
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• 1985

Factors affecting isolation and fusion of protoplasts of three Quercus species were investigated and procedures for isolation, purification and fusion of protoplasts of the three species were also established. Unhardened leaves and rapidly growing callus cultures were good source of viable protoplasts. The optimum composition of enzyme mixture for rapid isolation of protoplasts from leaf mesophyll tissues and calli was Cellulase Onozuka R-10 (20g/l, Macerozyme R-10(10g/l), Pectinase(250 units/l, $CaCl_2$, $2H_2O$(14mM), $MgSO_4{\cdot}7H_2O$(1.8mM), $KNO_3$(1.0mM), $H_3BO_3$(1.0mM), $KH_2PO_4$(0.2mM), KI($1.0{\mu}M$), 1,4-dithiothreitol (0.1mM) and mannitol (0.6M). Optimum density of protoplasts for maximum fusion was $2{\times}10^5/ml$ which was the highest protoplast density given in this study. Optimum concentration and duration of PEG 1450 treatment for inducing fusion appeared to be 29%(W/V) final PEG 1450 concentration and 5-10 minutes, respectively.

• Korean Journal of Plant Taxonomy
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• v.38 no.4
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• pp.573-582
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• 2008

We report a newly found natural habitat of Abeliophyllum distichum in mountainous slope range of Yeongdong-gun, Chungbuk Province. Abeliophyllum distichum Nakai is one of the Korean monotypic endemic species. Natural growth habitats of this species have been recorded from seven sites up to now, and all of the natural habitats are located in middle (Chungbuk Prov.) and middle west (Jeonbuk Prov.) parts of South Korea. Among the previously recorded seven natural habitats, six sites have been designated as Korean national monuments and protected with in situ conservation. New natural habitat of A. distichum is located on northwest slope of stiff hillock area beside the small stream, Seolgye-ri, Yeongdong-eup, Yeongdong-gun, Chungbuk Province. Total growing area is nearly $3,000 m^2$. It is 10-25 cm in soil depth and pH 5.0-6.5 in soil acidity in that area. And many of A. distichum are clustered with 2-5 individuals extended by stoloniferous asexual reproduction. And the total numbers of A. distichum are about 700 individuals with only typical white flowers, and the ratio between pin type and thrum type is 37% and 63%, respectively. The huge population of A. distichum is growing with Quercus mongolica-Fraxinus rhynchophylla association in a mixed forest, and it shows high affinity with Stephanandra incisa, Ligustrum obtusifolium, Euonymus alatus for. ciliatodentatus, and Smilax sieboldi.

• Korean Journal of Ichthyology
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• v.23 no.2
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• pp.119-127
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• 2011

Fish species composition in eelgrass bed of Geoje island, Korea was determined using monthly samples collected by a surf net from August 2009 to July 2010. A total of 31 species, 1,387 individuals and 4,776.5 g of fishes were collected. The dominant species were Rudarius ercodes, Gymnogobius heptacanthus, Ditrema temminckii, Takifugu niphobles, Hypodytes rubripinnis, Chaenogobius annularis, Aulichthys japonicus, Syngnathus schlegeli, Pterogobius elapoides which accounted for 89.3% of total number of individuals of fish collected. Eelgrass biomass showed a peak in August (DW 154 g/$m^2$), low values in November (DW 31 g/$m^2$) to February (DW 41 g/$m^2$), and started growing from May (DW 78 g/$m^2$). The biomass of fish increased by the catch of a large number of Gymnogobius heptacanthus, Chaenogobius annularis, Pterogobius elapoides, Rudarius ercodes and Pseudoblennius percoides in May and September when the eelgrass grew fast. Meanwhile, Syngnathus schlegeli, Urocampus nanus and Aulichthys japonicus were collected year-round with no clear relationship to the biomass of eelgrass bed.

• The Korean Journal of Mycology
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• v.41 no.3
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• pp.181-184
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• 2013

Rhodosporidium fluviale 201-C-1 and Kluyveromyces thermotolerans 150-JE-2(1) were isolated from wild flowers in Geumsan-gun, Chungcheongnam-do and Wando-gun, Jeollanam-do, respectively. Rhodosporidium paludigenum 86-J-1 and Candida sp. 80-J-3 were also isolated from flowers in Jeju island, Korea. Characteristics for these yeasts were not reported before. Therefore, morphological and physiological characteristics for these yeasts were investigated in this study. R. fluviale 201-C-1 was halophilic asporogenous yeast and formed pseudomycelium. It also grew at $30^{\circ}C$ and pH 4.0-6.0. K. thermotolerans 150-JE-2(1) was sugar-tolerant and halotolerant asporogenous yeast. It formed pseudomycelium and showed positive urease activity. Candida sp. 80-J-3 and R. paludigenum 86-J-1 were asporogenous yeasts, and grew well in yeast extract-peptone dextrose(YPD) medium, potato-dextrose medium as well as vitamin-free medium and YPD medium containing 5% NaCl.

• Korea journal of population studies
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• v.30 no.2
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• pp.117-146
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• 2007

The research reported in this paper suggests an index of a 'good job' and validates it in several different ways. Not much is known yet, it is emphasized, about what the defining characteristics of a good job are and what the causes and major consequences are resulting from the attainment of such job. This is not merely because relatively little attention has been paid to construct a usable index, but also because a few studies, if any, were often plagued with several limitations, some theoretical and other analytical. As a consequence, fragmented speculations and research findings tended to flourish in the shortage of an overarching conceptualization and rigorous empirical assessment. In particular, a comprehensive index that encompasses a few critical job characteristics based on some solid theoretical underpinnings was in thirsty want. To relieve this want, the current study tries to formulate such index and validate it. A covariance structure analysis of representative national sample survey (Korean General Social Survey) data in South Korea indicates that wage, occupational prestige, authority and job security are the defining characteristics of a good job and that the index consisting of these characteristics is generally valid with respect to its constituent attributes, antecedents and a consequence, thereby supporting its discriminant-convergent and construct validities. The findings are interpreted with providing a few substantive implications stemming from them.

• Journal of Korea Water Resources Association
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• v.43 no.4
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• pp.353-365
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• 2010

Generally, river discharge is measured at flood forecasting points, upstream dam points, large rivers, and important points over a basin, and it is hard to estimate discharge of medium or small stream and small catchment. Physically based rainfall-runoff model with geographical parameters can simulate discharge at all the points within a basin with optimized parameters for a point in the basin. In this study, GRM (Grid based Rainfall-runoff Model) calibrated at the outlet is applied. The discharge at upstream point is estimated and the possibility of model regionalisation is examined for ungauged catchment of small or medium stream within a river system. Wicheon and Boksu watershed in Nakdonggang (Riv.) and Yudeungcheon (Riv.) respectively are selected. The discharge at Miseong and Sindae station is simulated with the parameters estimated at Museong and Boksu station. The results of Miseong and Sindae station show good agreement with observed hydrographs in peak discharge and peak time and consistently linear relationships with high correlations in discharge volume, peak discharge, and peak time. And it shows GRM could be applied to estimate discharge at ungauged catchments along a river system.

• Journal of Life Science
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• v.20 no.7
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• pp.1005-1011
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• 2010

$\gamma$-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the central nervous system. GABA transporters (GATs) control extracellular GABA levels by reuptake of released GABA from the synaptic cleft. However, how GATs are regulated has not yet been elucidated. Here, we used the yeast two-hybrid system to identify the specific binding protein(s) that interacts with the carboxyl (C)-terminal region of GAT1, the major isoform in the brain and find a specific interaction with the ubiquitin-specific protease 14 (Usp14), a deubiquitinating enzyme. Usp14 protein bound to the tail region of GAT1 and GAT2 but not to other GAT members in the yeast two-hybrid assay. The C-terminal region of Usp14 is essential for interaction with GAT1. In addition, these proteins showed specific interactions in the glutathione S-transferase (GST) pull-down assay. An antibody to GAT1 specifically co-immunoprecipitated Usp14 from mouse brain extracts. These results suggest that Usp14 may regulate the number of GAT1 at the cell surface.

• Journal of Life Science
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• v.24 no.6
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• pp.612-617
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• 2014

The phenetic relationships among six natural populations of Equisetum pratense in Korea were investigated at the population level by constructing a tree based on Random Amplified Polymorphic DNA (RAPD) markers. RAPD analysis was also conducted to estimate genetic diversity and the population structure of E. pratense. A mean of 26.7% at the six population levels indicated polymorphism. E. pratense was found to have fewer alleles per locus (1.267) and fewer effective alleles per locus (1.176). Genetic diversity (0.102) in E. pratense is lower than the average for species with similar life history traits. Total genetic diversity values (HT) varied between 0.112 (OPD-07) and 0.445 (OPD-16), for an average overall polymorphic locus of 0.141. Inter-locus variation in the within-population genetic diversity ($H_S$) was low (0.102). Asexual reproduction, small population size, and the colonization process are proposed as possible factors contributing to the observed low genetic diversity in E. pratense. On a per-locus basis, the proportion of total genetic variation due to differences among populations ($G_{ST}$) ranged from 0.129 for OPD-07 to 0.455 for OPD-09, with a mean of 0.277. This indicated that about 27.7% of the total variation was among populations. Thus, genetic variation (72.3%) resided within populations. This study contributes new information for research on the taxonomy and population genetics of E. pratense.

• Journal of the Korean Geographical Society
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• v.42 no.6
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• pp.863-878
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• 2007

The time-spatial distribution of Scrub typhus and its relationship with environmental ecology, such as climate, and habitat change are analyzed in respect of the geography of disease. Scrub typhus was firstly reported in 1951, and reemerged in 1986 mainly in southwestern Korea. later it has sharply increased and spreaded out toward whole country in 1998 and 2004, except mid-eastern mountainous region. Hwasung City is the typical example of sudden upsurge of Scrub typhus. High incidence of Scrub typhus patients might due to elevated temperature and decreased precipitation during the summer, as well as milder autumn. Sharp increase of Scrub typhus patients at rural area since 1980's might also be the result of the rapid changes of land use pattern, which eventually have contributed for the active development of dense vegetation and propagation of chigger mites around cultivated land.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.1
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• pp.597-604
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• 2016

The aim of this study was to prevent the decrease in crop output by disease and insect pests and excessive spraying of agricultural pesticides by application uniformity. A 3m height and 15km/h speed is difficult to maintain with an unmanned helicopter for aerial application, which has been affected by the controlling habits and methods or environmental factors, such as changes in the wind. Therefore, in this study, an aerial application system was design to be attached to an unmanned helicopter, which can allow a controlled application width and spray rate automatically and verified experimentally using Rmax of MS-AVIATION. The size of agricultural land was 50 m2 and nine water sensitive cards were arranged at 1.25m intervals in 5 rows with each row having a 10m interval from the position of 5m. The unmanned helicopter was flying at speeds ranging from 7.2km/h to 17.6km/h and heights ranging from 2.32m to 3.47m. The proposed aerial application system allowed application uniformity by making a valid spraying area of 7.5 m2 with 46423 particles distributed on average.