• Title/Summary/Keyword: 무성아

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Isolation and Identification of Yarrowia lipolytica 504D producing Alkaline Proteinase (Alkaline Proteinase를 생산하는 Yarrowia lipolytica 504D의 분리 동정)

  • Kim, Chang-Hwa;Jin, Ingnyol;Yu, Choon-Bal
    • Korean Journal of Microbiology
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    • v.34 no.3
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    • pp.75-81
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    • 1998
  • The yeast strain 504D, isolated from salted shrimp soup, showed the best proteolytic activity under alkaline condition. The yeast formed vegetative cells in almost optimal media for yeasts, but formed only pseudohyphae in the MM medium containing citric acid and true hyphae in the MM medium containing N-acetylglucosamin and ${\beta}$-D-glucose. The yeast was classified as hemiascomycetes to form ascospores by sexual reproduction, and formed blastospores and athrospores by asexual reproduction. The yeast strain did not assimilate almost of the carbon sources, nitrate and nitrite, but some organic acids and alcohols. The fatty acids of whole cells were composed of 53.67% unsaturated fatty acids and 14.58% saturated, and, especially, C17:1 was observed in this strain but not in two control yeasts. However, almost of all results were very similar to the morphological and physiological characteristics of Yarrowia lipolytica KCCM 12495 and KCCM 35426, except for a little differences which are the composition of fatty acids and the manner of mycellium formation. Therefore, the isolated yeast strain 504D is identified as a Yarrowia lipolytica.

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Development of a Personal Robot Based on Modularization (모듈화 개념의 퍼스널 로봇 플랫폼 개발)

  • 최무성;양광웅;원대희;박상덕;김홍석
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2004.10a
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    • pp.742-745
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    • 2004
  • If a personal robot is popularized like a personal computer in the future, many kinds of robots will appear and the number of manufacturers will increase as a matter of course. In such circumstances, it can be inefficient, in case each manufacturer makes a whole platform individually. The solutions for this problem are to modularize a robot component (hardware and software) functionally and to standardize each module. Each module is developed and sold by each special maker and a consumer purchases desired modules and integrates them. The standardization of a module includes the unification of electrical and mechanical interface. In this paper, the standard interfaces of modules are proposed and CMR(Component Modularized Robot)-P2 made with the modules(brain, sensor, mobile, arm) is introduced. In order to simplify and to make the modules light, a frame is used for supporting a robot and communication/power lines. The name of a method and the way to use that are defined dependently on the standard interfaces in order to use a module in other modules. Each module consists of a distributed object and that can be implemented in the random language and platform. The sensor, mobile and arm modules are developed on Pentium or ARM CPU and embedded Linux OS using the C programming language. The brain module is developed on Pentium CPU and Windows OS using the C, C++ and RPL(Robot Programming Language). Also tasks like pass planning, localization, moving, object perception and face perception are developed. In our test, modules got into gear and CMR-P2 executed various scenarios like guidance, errand and guarding completely.

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Detection of Zymogenic ChsC Activity in Vegetative Hyphae of Aspergillus nidulans. (Aspergillus nidulans 영양균사에서 효소전구체형 ChsC 활성의 검출)

  • 박범찬;박윤희;박희문
    • Korean Journal of Microbiology
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    • v.40 no.2
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    • pp.178-182
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    • 2004
  • In the vegetative hyphae of Aspergillus nidulans, a zymogenic form of the class I chitin synthase activity was successfully measured by the assay condition for Saccharomyces cerevisiae class I chitin synthase, Chsl. The class I chitin synthase activity of the A. nidulans chsC wild type strain was increased about six-fold by trypsin-pretreatment, but that of the chsC disruption strain revealed no increase. Interestingly enough, level of the class I chitin synthase activity of the chsC disruption strain was almost the same as that of the chsC wild type without trypsin-pretreatment. These results indicated that the A. nidulans ChsC activity could be measured by account-ing the class I chitin synthase activity without the trypsin-pretreatment as an internal control. Consistence to the expression pattern of the chsC revealed by northern blot analysis, the activity of ChsC was increased upon reaching the culture time for acquiring developmental competence. Our results shown here also supported the previous report suggesting the possible involvement of ChsC in vegetative hyphal growth of A. nidulans.

Gamma Knife Radiosurgery on Uveal Melanoma - Cases Report - (포도막 흑색종에 대한 감마나이프 방사선수술 - 증례보고 -)

  • Kim, Byung Wook;Kim, Moo Seong;Sim, Hong Bo;Jeong, Yeong Gyun;Lee, Sun Il;Jung, Yong Tae;Kim, Soo Chun;Sim, Jae Hong;Youn, Il Han;Kim, Young Il;Paik, Koang Ook
    • Journal of Korean Neurosurgical Society
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    • v.30 no.5
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    • pp.652-656
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    • 2001
  • Uveal melanoma is uncommon but life-threatening intraocular malignancy and has been treated by irradiation, local excision and enucleation. Gamma-Knife radiosurgery allows a high dose of radiation to be delivered to an intracranial target with a very high spatial accuracy and has been used for the treatment of ocular melanomas. We have treated two cases of uveal melanoma between October 1994 and December 1999. They include one man and one woman(34, 62 years, respectively). They were followed up for 12 momths. Mean maximal dose was 65Gy. In one case, the tumor disappeared 7 months after gamma-knife radiosurgery. In another case, multiple tumors (uveal, suprasellar and cerebellar tumor) had decreased in size. These results show that single and high dose gamma-knife radiosurgery is may be an option in the local control of uveal melanoma which can spare the eyeball and vision.

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Network Topology Discovery with Load Balancing for IoT Environment (IoT환경에서의 부하 균형을 이룬 네트워크 토폴로지 탐색)

  • Park, Hyunsu;Kim, Jinsoo;Park, Moosung;Jeon, Youngbae;Yoon, Jiwon
    • Journal of KIISE
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    • v.44 no.10
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    • pp.1071-1080
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    • 2017
  • With today's complex networks, asset identification of network devices is becoming an important issue in management and security. Because these assets are connected to the network, it is also important to identify the network structure and to verify the location and connection status of each asset. This can be used to identify vulnerabilities in the network architecture and find solutions to minimize these vulnerabilities. However, in an IoT(Internet of Things) network with a small amount of resources, the Traceroute packets sent by the monitors may overload the IoT devices to determine the network structure. In this paper, we describe how we improved the existing the well-known double-tree algorithm to effectively reduce the load on the network of IoT devices. To balance the load, this paper proposes a new destination-matching algorithm and attempts to search for the path that does not overlap the current search path statistically. This balances the load on the network and additionally balances the monitor's resource usage.

Effect of Inoculation (Rhizobium meliloti) on the Plant Height and Dry Matter Yield in Seven Alfalfa (Medicago sativa L.) Varieties (알팔파(Medicago sativa L.) 7개 품종(品種)의 생육(生育)에 미치는 근류균접종(根瘤菌接種)의 영향(影響))

  • Kim, Moo-Sung
    • Korean Journal of Soil Science and Fertilizer
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    • v.8 no.1
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    • pp.37-43
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    • 1975
  • Effect of inoculation trial on plant height and dry matter yield of seven alfalfa varieties (Medicago sativa L. var. Washoe, Ranger, Lahontan, Narragensett, Atlantic, Vernal, and Moapa 69) which are considered to be well adapted in these areas were conducted during 1973-1974 in a relatively poor soil at the field station in Kyung Hee University. Root nodule bacteria: Rhizobium meliloti (Noculator Northrup Kings Co. Ltd) was applied at a rate to provide 6 oz/30l of alfalfa seeds. Seven varieties were seeded in pots on May 31, 1973 and in second year, they were transplanted in field. 1. Dry matter yield and plant height of all inoculation plots were significantly greater than those of the no inoculation plots in all varieties. 2. In the 1973, dry matter yield of inoculation plot a in pot experiment was in the order of Moapa 69> Vernal> Lahontan> Washoe> Atlantic> Narragensett> Ranger. 3. There was no difference in dry matter yield between with and without-inoculation after transplanted in a field in the second year (1974). Total dry matter yield of varieties was in the order of Washoe> Lahontan> Moapa 69> Ranger> Narragensett> Vernal> Atlantic.

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Sulfur Isotopic Ratios in Precipitation around Chonju-city, Korea and Its Availability as a Tracer of the Source of Atmospheric Pollutants (전주지역 강수의 황동위원소비와 대기오염원의 추적자로서 그 유용성)

  • Na, Choon-Ki;Kim, Seon-Young;Jeon, Sir-Ryeong;Lee, Mu-Seong;Chung, Jae-Il
    • Economic and Environmental Geology
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    • v.28 no.3
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    • pp.243-249
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    • 1995
  • In order to investigate the origin of sulfate in rain waters and to evaluate the feasibility of using sulfur isotope method as a tracer of atmospheric pollutants, the sulfur isotopic ratio of sulfate in rain waters collected in Chonju city from October 1994 to March 1995 was monitored and was compared with those of possible sources proposed by previous works. The pH of rain waters shows an intermediate acidic range from 4.45 to 6.88 and their daily variation appears to be well correlated with to the amount of precipitation. The sulfur isotopic ratios of sulfate in rain waters show a highly restricted range from 0.0 to + 1.8‰. The ${\delta}^{34}S$ values are similar to those of soil and pine tree surrounding Chonju city, but largely deviate from those of China. D-parameter($d={\delta}D-8{\delta}^{18}O$) of rain waters varies from 9.4 to 28.8. The values indicate that the rain waters in Chonju city are originated from the rainy front of China continent. All data obtained from this study suggested that sulfate in the rain waters collected in Chonju city was mainly derived from the sulfur dioxide gas emitted by the petroleum combustion. Therefore, sulfur isotopic study for the precipitation provided an excellent tool for environmental assessment in this region and for tracing the source of atmospheric pollutants.

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The Heavy Metal Adsorption Properties of Hydroxyapatite Powders Synthesized by Precipitation Reaction Method and Its Applicability for the Removal Agents of Noxious Metallic Ions in Waste Water (침전반응법으로 합성한 수산아파타이트 분말의 중금속 흡착 특성 및 폐수중의 유해 금속 제거제로서의 유용성)

  • Lee, Mu Seong;Na, Choon Ki;Lee, Mi Suk;Kim, Oak Bae;Kim, Moon Young
    • Economic and Environmental Geology
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    • v.28 no.3
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    • pp.231-241
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    • 1995
  • It is well known that hydroxyapatite [$Ca_{10}(PO_4)_6(OH)_2$] have an exchangeability for various heavy metal ions in aqueous solution. To evalute the feasibility of employing the synthetic hydroxyapatites as an eliminatable exchanger for environmentally noxious caions in waste water, the adsorption properties, the removal capacities and the selectivity of the apatites for various cations were investigated in more detailed. The heavy metal cations have been exchanged in calcium part of hydroxyapatite. The order of the degree of amount exchanged of the investigated cations is $Pb^{2+}>Cd^{2+}>Zn^{2+}>Ba^{2+}$. The molar ratios between released $Cd^{2+}$ ions and remeved divalent metal cations in the reacted solution with hydroxyapatite are roughly close to an integer 1.0, suggesting that an ion-exchange reaction could have played a major role in the removal of heavy metals rather then an adsorption effect. The exchangeability of the hydroxyapatite powder of Ca/P molar ratio 1.67, which have specipic surface area of $104.5m^2g^{-1}$, appeared to be better then $237.6{\mu}g$ per g for $Pb^{2+}$ ions. The removal capacity of the heavy metal ions varies directly as particle size of hydroxyapatites. All evidences obtained indicate that the synthesized hydroxyapatite powders by precipitation reaction method can be employed as an effective cation exchanger for eliminating noxious ions in waste water even in some improvemental.

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The Monomyth Structure of Méliès Films and the Way of Story (멜리에스 영화의 원형신화 구조와 이야기의 길)

  • Lee, Won-Ik
    • The Journal of the Korea Contents Association
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    • v.17 no.10
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    • pp.463-471
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    • 2017
  • There is a world of unconscious that works when creating story contents. The unconscious layer of the abyss has the artist goes on the path of creating a common story structure without knowing because the human race is connected. It is monomyth and is now used as an important authoring tool. Analyzing the structure of the silent film "A trip to the Moon" made before the discovery of it, we can still find the frame of monomyth. Although this film is an intuitive imagination of the inspiration received from four contemporary works, the monomyth has been completely revealed.. This means it's still working regardless of area or time. That's why the story that was created in a modern short time has the same structure as the myth that passed down for a long time because there is archetype of story in human collective unconscious. This archetype determines the way the story. The human brain is not infinitely free to imagine but that it can be applied to the surface along the path of the archetype. Good story contents is less likely to succeed if it does not follow the path proposed by the archetype within our collective unconscious.

The Role Change and Geographical Inertia of Central Places : A Case Study Chil-Bo Area, Jeon-Buk (지역 중심지의 역할 변화와 지리적 관성 -전라북도 정읍시 칠보 지역을 사례로-)

  • Cho, Sung-Wook
    • Journal of the Korean association of regional geographers
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    • v.12 no.5
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    • pp.571-582
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    • 2006
  • This study look into a role change and geographical inertia of the central place. The place is selected and progressed as the center which is the joining of physical condition and social situation of the region. The central place is the role change according to social change but it has geographical inertia as the central place. The result is as follows. Firstly, the role of regional center is changed. This sample region is the center of a national defense and administration and traffic in Three-Country period. But the role of the national defense is disappeared with the unity of the three countries. A traffic center facility was lost because of traffic axis changed from the east-west line to the north-south line when Ko-Ryo period. A facility loss as a traffic center leads to diminish the administration function and the movement of the regional center within that area. Secondary. the new regional center function was maintained by the joining of an outside and inside peoples. It maintained the geographical inertia by Confucianism culture instead of administration function. Thirdly, it had geographical location movement within the area.

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