• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.180-185
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• 2015

Leaf discs from 'Yeobong' and 'Maehyang' strawberry plants were used as explants for transformation. The Agrobacterium tumefaciens strain EHA105 harboring the monellin gene under the control of the CaMV 35S promoter was used in co-cultivation experiments. The frequencies of callus formation and plant regeneration from leaf explants after co-cultivation in 'Yeobong' were higher than those of 'Maehyang'. These transgenic plants showed normal growth patterns and flowering. PCR and Southern hybridization confirmed that 1 to 2 copies of the monellin gene were integrated into genome of the transgenic strawberry plants. Northern blot analysis confirm that the transcripts were expressed in transgenic strawberry plants. Although long-term subcultured transgenic strawberry plants showed a phenomenon to escape the transgene, the transformation system established in this study provides new opportunities for genetic improvement of strawberry plants.

• Journal of Korean Ophthalmic Optics Society
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• v.20 no.1
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• pp.9-14
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• 2015

Purpose: In this paper, the changes in eyeglasses frames were investigated when the frames were exposed to artificial sweat. Methods: The changes in surface, hardness, and tensile strength of eyeglasses frames made of nickel silver, Monel, and titanium were tested after the exposure to the artificial sweat of pH 6.5, 6.0, and 5.0, during 1, 3, and 6 week(s), for each. Results: Corrosion pattern of eyeglasses frames made of nickel silver, Monel, and titanium was induced by artificial sweat, and the patterns were different from another frame materials. In proportion to the pH of artificial sweat and exposure time to artificial sweat, hardness of all frames made of three different material decreased, however, tensile strength of the three frames was not changed. Conclusions: The necessity of routine management for eyeglasses frames can be suggested since the changes in surface corrosion and hardness of eyeglasses frames induced by sweat were shown.

• The Journal of the Korean Society for Microbiology
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• v.9 no.1
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• pp.1-5
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• 1974

The authors identified 426 cultures of the Genus Salmonella among 1644 suspectable cultures of enteric pathogens submitted, to be bacteriologically confirmed, by the eleven hygiene laboratories of cities and provincial level and some hospital laboratories in 1973. According to the results obtained from the physical and biochemical tests, and the antigenic structural analyses, eleven cultures of Salmonella paratyphi B, nineteen cultures of Salmonella typhimurium, one each culture of Salmonella potsdam and Salmonella newport, sixteen cultures of Salmonella enteritidis and three hundred thirty-four cultures of Salmonella typhi were confirmed. The drugs of choice for treating Salmonella infections were found to be chloramphenicol, ampicillin and gentamycin in Korea, although there were a few cultures appearing in a couple of previous years which showed resistant patterns by the In Vitro tests.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.11
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• pp.42-50
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• 2016

Overlay welding affects the chemical components and weld hardness by dilution of the lamination layer thickness, which determines the surface properties. This study experimentally investigates different numbers of layers for overlay welding monel materials, which are anti-corrosion materials. The Fe content, weldability of the base metal and monel materials, hardness, and surface flatness were examined. Each evaluation was carried out after overlay welding with three layers on the base material and pipe base material of the plate. The Fe content was evaluated by analyzing the constituents of each layer. The Fe content was satisfactory in the three layers. The weldability of the laminate specimens was evaluated by a bending test. The hardness and bead flatness of the laminate specimens were evaluated by micro Vickers and 3D measurements. The hardness was highest in the heat-affected zone with one layer, and it decreased with increasing lamination. In the case of bead flatness, there is a sharp difference in the deviation with increasing numbers of laminations, which should be considered carefully.

• KSBB Journal
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• v.13 no.5
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• pp.535-539
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• 1998

The monellin, a sweet-taste protein, was expressed and secreted in Saccharomyces cerevisiae. The secreted menellin was concentrated using an ultrafiltration membrane with a nominal molecular weight cut off of 3,000 or by ammonium sulfate precipitation. The monellin was purified by G-25 gel filtration chromatography, followed by CM-Sepharose ion exchange chromatography. The purified monellin was characterized by SDS-PAGE (SDS-Polyacrylamide Gel Electrophoresis) and PHLC. The molecular weight of monellin was found to be 10,700 dalton, and its purity was over 95%.

• Monthly Duck's Village
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• s.56
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• pp.46-52
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• 2008

최근 오리 농가수 및 사육수수의 급격한 증가와 규모화가 빠르게 진행되어 왔으나 각종 질병(전염성질병, 비전염성질병, 중독증 등)에 대한 피해가 크게 발생되고 있지만 이들 질병들이 어느 때 얼마만큼 피해가 주고 있는지 상황파악이 아주 미흡한 상태이다. 이렇게 농가수와 사육수가 늘어난 근본적인 이유는 외식산업의 발달과 붉은 고기이면서도 건강에 좋다는 선호도가 높아지면서 오리산업의 외형적 성장에 그 기반을 두고 있다. 오리에서 발생되는 질병으로는 오리패혈증(리메렐라 감염증), 오리 바이러스성 간염, 대장균증, 살모넬 라감염증, 마이코플라즈마병, 오리바이러스성 장염, 조류인플루엔자, 보튤리눔중독증 등 다양하며 국내에서는 오리바이러스성 장염을 제외한 위에 기술된 모든 질병이 현재 발생되고 있는 것으로 추정되고 있다. 위에 기술된 질병들 중에서 현재 가장 문제가 되고 있는 질병으로는 오리패혈증과 오리 바이러스성간염이다.

• The Journal of the Korean Society for Microbiology
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• v.11 no.1
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• pp.19-22
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• 1976

The authors identified 436 Salmonella cultures, which were 426 Salmonella typhi and ten cultures of other serotypes among 2033 suspectable enteric pathogens collected from all over the country in 1975.

• The Journal of the Korean Society for Microbiology
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• v.8 no.1
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• pp.1-5
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• 1973

The authors identified 833 Salmonella cultures collected from various parts of the country in 1972. The procedures for the morphological and biochemical tests and for the serological determinations were performed by means of the conventional screening methods recommended by the National Center for Disease Control in U.S.A. The results of the laboratory tests were summerized as follows: 1. Of 833 Salmonella cultures, 1 S. paratyphi A, 1 S. nitra, 1 S. kiel, 1 S. abortusequi, 6 S. paratyphi B, 5 S. abony, 4 S. caledon, 13 S. typhimurium, 2 S. coeln, 1 S. oranienburg, 1 S. thompson, 1 S. bonn, 1 S. gabon, 1 S. colorado, 1 S. richmond, 2 S. berta, 20 S. enteritidis, 1 S. regent, 1 S. london were identified besides 769 cultures of S. typhi. 2. The antibiotics sensitivity tests by means of Ericsson's disc method using seven kinds of antibiotics were carried out, i.e. chloramphenicol, ampicillin, which were widely in common use in the country and the results were compared with that of Salmonella cultures isolated in 1971 as shown in table 4.

• Journal of the Korean Society of Industry Convergence
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• v.25 no.6_2
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• pp.961-968
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• 2022

Hydrogen embrittlement leads to the damages in bolts, nut, especially, high pressure valves, in the semiconductor facilities, hydrogen vehicles, hydrogen stations and so on. Monel material has higher strength than SUS material. Therefore, even though Monel material with special alloy is usually used to prevent the hydrogen embrittlement, it needs powerful drawing system to manufacture the rectangular or hexagonal bar using circular bar. The purpose of this study is to investigate the characteristics of plastic deformation of Monel material and 2 rollers of rolling unit in plastic limit through numerical analysis. As the results, it was predicted that, based on mean stress, as the rolling step was increased, the rolling force of rolling unit was decreased. In addition, the heat treatment for Monel material was needed because of residual stress due to plastic deformation. As for rollers, the roller was safe about 1.86 times compared with that of ultimate strength. In this study, as the roller 2 showed larger stress than roller 1, thus, roller 2 should be designed carefully to guarantee the safety. Further it was confirmed that the reaction force of roller could be helpful in bearing design.

• Journal of the Korean Society of Industry Convergence
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• v.27 no.3
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• pp.573-580
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• 2024

Recently, the special alloy, for instance, such as Monel and Inconel, is used for valves, bolt/nuts, and fittings in semiconductor facility, FCEV(fuel cell electric vehicle) and hydrogen gas station, to reduce the hydrogen embrittlement. Even though the Monel material has high cost, it is recommended to use for the cases of ultra high pressure, ultra high leak-proof and so on. The purpose of this study is to investigate the characteristics of Monel material within elastic limit through the comparative analysis when Monel material is extruded or drawn. As the results, the deformation of Monel material was increased as the number of pass was increased, further, the deformation of Monel material by drawing was larger than that by extrusion. In the safety factor, the case that load is less than 420kN, the plastic deformation due to drawing could be happened faster than that due to extrusion. However, the case of more than 420kN, it showed that the plastic deformation for extrusion and drawing was almost similar.