• Korean Journal of Agricultural Science
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• v.12 no.1
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• pp.22-30
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• 1985

Protoplasts of Pisum sativum L. were isolated and cultured from leaf mesophyll tissue. The successful yield of protoplast was obtained in an enzyme solution of 2% 'Onozuka R-10' and 2 % 'Macerozyme R-10' contained 6mM $CaCl_2{\cdot}2H_2O$ within 4 hours. They were divided in B5 culture medium supplemented with 2mg/l kinetin, 1mg/l 2, 4-D and 0.2% Difcobacto agar. Divisions of the protoplasts were continued and led to colony formation for 1 months. The colony from protoplasts of pea mesophyll tissue was formed to callus after subculture in a medium contained macronutrients and amino acids of BII medium and micronutrients and vitamins of B5 medium, and also supplemented with 2mg/l kinetin 2mg/l NAA.

• Journal of Life Science
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• v.23 no.3
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• pp.341-346
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• 2013

The disappearance of seaweed flora in some rocky areas, which is known as algal whitening, barren ground, coralline flats, or deforested areas, is associated with some species of coralline algae. To determine the biological characteristics of a representative species of crustose coralline alga, the 18S rDNA gene was sequenced to identify the genus Lithophyllum. According to its morphological and distributional characteristics, it was deduced to be L. yessoense. Viability was measured using triphenyl tetrazolium chloride and showed high viability from December to February. Culture conditions of $16^{\circ}C$, a 16 hr light, 8 hr dark cycle, and 30 ${\mu}E/m^2/s$ light intensity were optimal for maintaining the viability of the alga for up to five days. Included in the fatty acids was 9.7% ${\omega}$-3 eicosapentaenoic acid. An electron microscopy scan of the surface structure revealed round craters about 3.6 ${\mu}m$ in diameter, which were covered with rough, irregular, and angular polygon-shaped structures about 1.0 to 3.7 ${\mu}m$ in size. Based on the composition and structure found in our study, biomimetic coralline alga might become an environmentally friendly antifouling material against the attachment of soft foulants.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.46 no.2
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• pp.40-47
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• 2009

This paper proposes a method for vehicle license plate detection using mathematical morphology and symmetry. In general, the shape, color, size, and position of license plate are regulated by authorities for a better recognition by human. Among them, the relatively big intensity difference between the letter and the background region of the license plate and the symmetry about the plate are major discriminating factors for the detection. For the first, the opened image is subtracted from the closed image to intensify the region of plate using the rectangular structuring element which has the width of the distance between two characters. Second the subtraction image is average filtered with the mask size of the plate. Third, the column maximum graph of the average filtered image is acquired and the symmetry of the graph is measured at every position. Fourth, the peaks of the average filtered image are searched. Finally, the plate is assumed to be positioned around the one of local maxima nearest to the point of the highest symmetry. About 1,000 images taken by speed regulation camera are used for the experiment. The experimental result shows that the plate detection rate is about 93%.

• Journal of the Korea Computer Graphics Society
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• v.23 no.2
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• pp.11-22
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• 2017

This study proposes IMToon(IMage-based carToon) which is an image-based cartoon authoring system using an image processing algorithm. The proposed IMToon allows general users to easily and efficiently produce frames comprising cartoons based on image. The authoring system is designed largely with two functions: cartoon effector and interactive story editor. Cartoon effector automatically converts input images into a cartoon-style image, which consists of image-based cartoon shading and outline drawing steps. Image-based cartoon shading is to receive images of the desired scenes from users, separate brightness information from the color model of the input images, simplify them to a shading range of desired steps, and recreate them as cartoon-style images. Then, the final cartoon style images are created through the outline drawing step in which the outlines of the shaded images are applied through edge detection. Interactive story editor is used to enter text balloons and subtitles in a dialog structure to create one scene of the completed cartoon that delivers a story such as web-toon or comic book. In addition, the cartoon effector, which converts images into cartoon style, is expanded to videos so that it can be applied to videos as well as still images. Finally, various experiments are conducted to verify the possibility of easy and efficient production of cartoons that users want based on images with the proposed IMToon system.

• Journal of the Korea Concrete Institute
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• v.20 no.4
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• pp.513-522
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• 2008

The fiber dispersion performance in fiber-reinforced cementitious composites is a crucial factor with respect to achieving desired mechanical performance. However, evaluation of the fiber dispersion performance in the composite PVA-ECC (Polyvinyl alcohol-Engineered Cementitious Composite) is extremely challenging because of the low contrast of PVA fibers with the cement-based matrix. In the present work, an enhanced fiber detection technique is developed and demonstrated. Using a fluorescence technique on the PVA-ECC, PVA fibers are observed as green dots in the cross-section of the composite. After capturing the fluorescence image with a Charged Couple Device (CCD) camera through a microscope. The fibers are more accurately detected by employing a series of process based on a categorization, watershed segmentation, and morphological reconstruction.

• Journal of IKEEE
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• v.19 no.2
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• pp.193-200
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• 2015

In this paper, we propose the radiation measuring algorithm and the device composition using CMOS image sensor. The radiation measuring algorithm using CMOS image sensor is based on the radiation particle distinguishing algorithm projected to the CMOS image sensor and accumulated and average number of pixels of the radiation particles projected to dozens of images per second with CMOS image sensor. The radiation particle distinguishing algorithm projected to the CMOS image sensor measures the radiation particle images by dividing them into R, G and B and adjusting the threshold value that distinguishes light intensity and background from the particle of each image. The radiation measuring algorithm measures radiation with accumulated and average number of radiation particles projected to dozens of images per second with CMOS image sensor according to the preset cycle. The hardware devices to verify the suggested algorithm consists of CMOS image sensor and image signal processor part, control part, power circuit part and display part. The test result of radiation measurement using the suggested CMOS image sensor is as follows. First, using the low-cost CMOS image sensor to measure radiation particles generated similar characteristics to that from measurement with expensive GM Tube. Second, using the low-cost CMOS image sensor to measure radiation presented largely similar characteristics to the linear characteristics of expensive GM Tube.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.37A no.12
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• pp.1054-1064
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• 2012

HDR (high dynamic range) tone mapping algorithms are used in image processing that reduces the dynamic range of an image to be displayed on LDR (low dynamic range) devices properly. The retinex is one of the tone mapping algorithms to provide dynamic range compression, color constancy, and color rendition. It has been developed through multi-scale methods and luminance-based methods. Retinex algorithms still have drawbacks such as the emphasized noise and desaturation. In this paper, we propose a multi scale tone mapping algorithm for enhancement of contrast, saturation, and noise of HDR rendered images based on visual brightness functions. In the proposed algorithm, HSV color space has been used for preserving the hue and saturation of images. And the algorithm includes the estimation of minimum and maximum luminance level and a visual gamma function for the variation of viewing conditions. And subjective and objective evaluations show that proposed algorithm is better than existing algorithms. The proposed algorithm is expected to image quality enhancement in some fields that require a improvement of the dynamic range due to the changes in the viewing condition.

• Cartoon and Animation Studies
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• s.36
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• pp.349-374
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• 2014

The light is the language. Directors have to describe the scene component effectively his intention to configure the scene as an appropriately. After this act of the character, the layout of the props and scene lights will enter to the scene components. Those things help to audiences can understand narrative of work and emotion that producer want to send. Expressing their emotions especially using the lights by adjusting the colors and contrast makes audience to concentrate on work and understand naturally. This lighting technique clearly appears on early year theaters stage of England and Rembrandt's paintings. Properly dividing and controlling the lights dramatically increases the beauty of the work elements to express a variety of emotions such as worries and fear. Therefore, it can be evolve depending on director's intent of using lights on his work. Lights can increase involvement of human emotion through basic features that cognition of object, visualization of space-time and by artistic method in the product. This study will examine the role and how to use lighting to express the proper sentiment based on the narrative of the work. Making research named "Lighting Research of 3D animated film which applying light features to express emotion" previous study and have to combine emotional vocabulary and emotion-based theory for classifying the emotional language that can be applied on 3D animation. And choosing most emotional scene from 3D animation for analyze how they used lighting to expressing emotions. Directors trying to show up about the light role through light method that matched perfectly with an emotional language. Expecting this research work of directing 3D animations light for expressing emotional feelings will be continue successfully.

• Economic and Environmental Geology
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• v.34 no.2
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• pp.227-241
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• 2001

Several $Co_2$-rich springs in the Chungcheong area, Le., the Angsung spring, the Chojung spring, the Myungam spring, the Bukang spring and the Daepyung spring have been long known for their particular water chemistry. The occurrences of these springs are closely related to the geologic structure of Mesozoic granite such as dyke swarms, fault zones and the geologic boundary between granite and its adjacent gneiss. The $Co_2$-rich water samples show a high $Co_2$ concentration ($P_{CO2}$ 0.25 atm to 0.99 atm), weak acidic pHs and the electrical conductivity ranging from 101 to 2,950 ${\mu}$S/cm. The $Co_2$-rich water samples are classified into the Ca-$HC0_3$ type and the Ca(Na)-$HCO_3$) type in chemical composition. Environmental isotopic data $^{2}H/^{1}H, ^{18}O/^{16}O$) indicated that $Co_2$-rich water was meteoric origin. The ${\delta}^{13}C$ values of $Co_2$-rich water range from -3.1$\textperthousand$ to -6.8$\textperthousand$ PDB. The values indicate that $H_2CO_3^0$ and $HC0_3^-$ of the water samples are mainly originated from a deep-seated source and partly contributed from carbonatc minerals. The major minerals determining the chemistry of $Co_2$-rich watcr arc probably the carbonate minerals which are present as veins and secondary minerals, and the plagiocalse in granite and gneiss.

• The Korean Journal of Pharmacology
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• v.20 no.2
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• pp.23-34
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• 1984

To investigate diurnal variations of opiate receptor binding and its modification by experimental condition or treatment of various centrally-acting drugs, the amount of maximum $^3H-morphine$ binding in rat midbrain homogenates was measured at 4 hour intervals for 24 hours. Animals were conditioned under the controlled L : D, 12 : 12 cycle or D: D, 12 : 12 cycle, for 3 weeks and treated with 0.5 ml of physiological saline or drugs for 2 weeks. A highly significant diurnal rhythm with peak at 22 hour of early dark phase with an amplitude$(0.68{\pm}0.06\;pmole/mg\;protein)$ of +51.1% and nadir $(0.33{\pm}0.03\;mole/mg\;prtein)$ at 18 hour of late light phase with an amplitude of -26.6% was found in control group. 24 tour mean of $^3H-morphine$ binding was $0.45{\pm}0.03\;pmole/mg$ protein respectively. Constant dark adaptation or treatment of reserpine, pargyline, imipramine, amphetamine and chlorpromazine modified the diurnal rhythm in the time of peak and nadir binding shape, phase, amplitude of the diurnal curve and 24 hour mean of $^3H-morphine$ binding. However, Kd values were not changed in all experimental groups : Statistical analysis at times of least and great binding indicates that the differences in $^3H-morphine$ binding were due to changes not in the affinity, but in the number of binding sites. The results are interpreted with regard to the diurnal rhythm of opiate receptor finding. The modes of action of psychoactive drugs are closely related to postulated changes of receptor sensitivity in neuropharmacological aspects.