• Journal of Life Science
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• v.16 no.4
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• pp.605-611
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• 2006

To investigate the possible use of probiont in fish farming industry, a bacterium with inhibitory effect against Vibrio anguillarum was isolated from gastrointestinal tract of the marine fish of yellow tail. It was identified to be Bacillus amyloliquefaciens H41 based on biochemical and physiological characterization. The optimal growth conditions of the isolated strain were 1% peptone, 1.5% yeast extract, 1% sucrose, 0.5% NaCl, 0.05% $MgSO_4{\cdot}7H_20$, pH 7.0-8.0, and 20 hr of incubation between $28-35^{\circ}C$ under aeration. The culture supernatant of the isolated strain showed inhibition activity against V. anguillarum. Inhibition activity was cleared by forming a clear zone by a paper-disk method. The maximal production of growth inhibition factor was induced by cultivation under 1% peptone, 1.5% yeast extract, 1% sucrose, 1% NaCl, 0.05% $MgSO_4{\cdot}7H_20$, pH 7.5 and at $35^{\circ}C$. The highest growth inhibition factor production was observed after 16-24 hr cultivation under aeration. The culture supernatant of the isolated strain showed inhibition activity whereas no inhibition activity was shown from the standard B. amyloliquefaciens KCTC 1724 strain. The growth inhibition affected only against V. anguillarum among other pathogenic Vibrios tested here.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.36 no.4B
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• pp.332-345
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• 2011

As weapon systems are evolving into more advanced and complex ones, the role of the software is becoming heavily significant in their developments. Particularly in the war field of today as represented by the network centric warfare(NCW), the reliability of weapon systems is definitely crucial. In this context, it is inevitable to develop software reliably enough to make the weapon systems operate robustly in the combat field. The reliability engineering activities performed to develop software in the domestic area seem to be limited to the software reliability estimations for some projects. To ensure that the target reliability of software be maintained through the system's development period, a more systematic approach to performing software reliability engineering activities are necessary from the beginning of the development period. In this paper, we consider the software reliability in terms of the development of a weapon system as a whole. Thus, from the systems engineering point of view, we analyze the models and methods that are related to software reliability and a variety of associated activities. As a result, a process is developed, which can be called the software reliability engineering process for weapon systems (SREP-WS), The developed SREP-WS can be used in the development of a weapon system to meet a target reliability throughout its life-cycle. Based on the SREP-WS, the software reliability could also be managed quantitatively.

• Microbiology and Biotechnology Letters
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• v.48 no.2
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• pp.215-222
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• 2020

The marine microorganism PX-1, which can hydrolyze xylan, was isolated from coastal sea water of Jeju Island, Korea. Based on the 16S rRNA gene sequence and chemotaxonomy analysis, PX-1 was identified as a species of the genus Aestuariibacter and named Aestuariibacter sp PX-1. From the culture broth of PX-1, an extracellular xylanase was purified to homogeneity through ammonium sulfate precipitation and subsequent adsorption chromatography using insoluble xylan. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration chromatography estimated the molecular weight of the purified putative xylanase (XylA) as approximately 64 kDa. XylA showed xylanase activity toward beechwood xylan, with a maximum enzymatic activity at pH 6.0 and 45℃. Through thin-layer chromatographic analysis of the xylan hydrolysate produced by XylA, it was confirmed that XylA is an endo-type xylanase that decomposes xylan into xylose and xyloligosaccharides of various lengths. The K_m and V_max values of XylA for beechwood xylan were 27.78 mM and 78.13 μM/min, respectively.

• Journal of Korea Technology Innovation Society
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• v.15 no.4
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• pp.745-765
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• 2012

When attempting to use the income approach for the purpose of technology valuation, it is essential to identify the economic life of the technology in question. From the mid-2000s up to the present, the methods proposed by major Korean institutions for estimating the economic life of technologies have been based on cited patent life (CLT), which is one of the types of technology life. The present study utilizes cited patent life (CLT) to estimate the economic life of technology for the purpose of technology valuation, and proposes a new method of analyzing cited patent life, a method that has been improved by taking into consideration the elapsed period and the time period of investment required for commercialization, two factors which have been hitherto overlooked. Survival curve analysis is a method that has already been widely utilized to estimate the economic life of tangible assets, and this study applies the same method to the calculation of the cited patent life index of technology to provide a more objective method for determining the lifetime of a technology. The remaining life expectancy of cited patent life based on the number of elapsed years was calculated and used to determine the life expectancy of a technology that has reached a specific number of elapsed years, which is referred to as the remaining cited-patent life time (r-CLT).

• Research in Plant Disease
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• v.14 no.1
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• pp.43-50
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• 2008

Rhizoctonia blight (large patch) caused by Rhizoctonia solani AG2-2 is one of the major diseases on zoysiagrass in golf courses. In this study, anatgonistic bacteria to R. solani AG2-2 were selected in vitro tests using confrontation bioassay and triple layer agar diffusion method. The most active bacteria, Bacillus subtilis CJ-9 were tested for controlling large patch in pots. Relative Performance Indies (RPI) was used as a criterion for the selection of potential biocontrol agent. B. subtilis CJ-9 showed resistance to major synthetic agrochemicals used in golf course. In field tests at golf course, B. subtilis CJ-9 was more effective in suppression of large patch severity and population development of R. solani AG2-2 in soil than chemical fungicides. B. subtilis CJ-9 could be an alternative to chemical fungicides for eco-friendly management of large patch on zoysiagrass.

• Journal of Life Science
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• v.21 no.2
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• pp.235-241
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• 2011

The purpose of this study was to isolate soy curd forming bacterial strains. Soy curd forming bacteria were isolated from Kimchi, a traditional Korean vegetable food that is fermented using lactic acid bacteria. Among 196 bacterial strains, ten isolates (strain No. 2-2-2, 2-15-2, 2-18-1, 2-19-2, 3-4-1, 3-4-2, 3-8-1, 3-8-3, 3-17-1, 4-39-5) formed firm soy curd. The isolated bacterial strains were identified by molecular biological and biochemical analyses. The genomic DNAs extracted from the isolated bacterial strains were used as a template for PCR amplification of 16S rDNA region. By comparing the results of the 16s rDNA sequences with GenBank data, the isolated strains were identified as Leuconostoc mesenteroides group and Lactobacillus sakei group. The phylogenetic position of soy curd forming strains and their related taxa were investigated using neighbor-joining method. L. mesenteroides group was further identified as L. mesenteroides subsp. dextranicum based on biochemical properties. L. sakei group was named Lactobacillus sp., because it showed a variety of biochemical properties.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.149-154
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• 2015

A new Phalaenopsis cultivar 'SM 333' was bred by Sangmiwon Orchid, Korea, which produces young plants through tissue culture techniques. The new cultivar 'SM 333', showing the phenotype of multiflora with pink color and small, multibranching-type characteristics, was derived from crossing between Phalaenopsis 'Odoriko' and 'Be Tris'. An elite individual number '02-03-33' later termed 'SM 333' was selected among about 300 individual progenies, based on an intensive selection process covering vegetative and flowering distinctiveness over more than 2 years. In year 2004-2005, the 1st and 2nd characteristic analyses were carried out through performance and uniformity tests. 'SM 333' shows flower color that is bright clean pink (RHS # RP69D) and flower shape that is formal type with 5.0 and 5.8 cm in flower height and width, respectively. 'SM 333' is regarded as raceme flower type suitable for the small casual flower market. The leaves of 'SM 333' grow horizontally and about 20.8 cm in length and 6.5 cm in width. This cultivar also possesses no genetic variation, and is amenable to fast in vitro propagation and easy growth due to its vigorous growth habit. This 'SM333' was registered (Reg. # 2916) with Korea Seed & Variety Service (KSVS) on 1st December, 2009, and the plant breeder's right is currently controlled by Sangmiwon Orchid Company, Korea.

• Korean Journal of Family Social Work
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• no.23
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• pp.263-302
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• 2008

The study had four purposes as follows; First, the researcher tried to find the characteristics of conflict. Second, the researcher attempted to find the factors influencing the conflict between mother and the eldest son, Third, the study attempted to find the therapist's intervention techniques, which were caused the change of the conflict between mother and the eldest son. Lastly, the study was to find the changed contents of family relationships. The total family counseling was 13 sessions, which included individual and family counseling. The study used constant comparative analysis by using open coding method to find the factors. Also, the researcher used Miles and Huberman's matrix and graphical network display to show the relationships among factors influencing the conflicts between mother and the eldest son. The study found the boundary problem of family, family's undifferentiated ego, dysfunctional communication patterns, and mother's experience from family of origin as factors influencing the conflict between mother and the eldest son. On the other hand, the study found that the therapist used the formation of therapeutic alliance relationship, guide of communication skills, the reflection of repeated pattern, reframing and a metaphor as intervention methods. The result of study revealed that changed level of family relationship, activating functional communication, changed boundary among family members, differentiated ego of family members, and changed cognition as the changed contents of family relationship.

• Journal of agriculture & life science
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• v.46 no.4
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• pp.41-55
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• 2012

On the basis of vegetation data collected by point-centered quarter method in analysis in Baekdudaegan of Gangwon province in the area of Hyangrobong, Odaesan, Seokbyeongsan, Dutasan, Deokhangsan and Hambaeksan, the study was carried out to classify forest communities and to evaluate the successional trends. The classification method of cluster analysis was used to make various disordered forests into several common groups for 1,004 sample points all together. By clustering the forests in the six study areas were classified into 28 forest communities, which were subjected to aggregate 8 representative forest communities on the count of species composition and species diversity. They were Mesophytic mixed forest community, others deciduous forest community, Quercus mongolica (dominant) community, Q. mongolica (pure) community, Pinus densiflora - Q. mongolica community, P. densiflora community, Betula ermanii community and Q. mongolica - Pinus koraiensis community. The ecological outlook from the result indicated that P. densiflora community and P. densiflora - Q. mongolica community, which were located in Seokbyeongsan, Dutasan and Deokhangsan around 1,000m above the sea level showed lower species diversity index. On the contrary Mesophytic mixed forest community, others deciduous forest community which was located in Hyangrobong, Odaesan and Hambaeksan mostly in protected area and national park around 1,500m above the sea level displayed higher species diversity index. As the composition ratio of Q. mongolica within a certain community was decreased, the species diversity was generally increased, assumed that abundance of Q. mongolica might be negatively associated with species diversity in the national deciduous forest.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.15 no.6
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• pp.1-26
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• 2020

The term 'Unicorns' in the corporate ecosystem was firstly introduced by Aileen Lee in 2013. It has been actively discussed in South Korea particularly to compare the level of the 'start-up ecosystem' from a global perspective. Accordingly, the Korean government has recently set a policy goal 'to nurture 20 Korean unicorn companies by 2022'. While the phenomenon of 'Unicorn Club Company' has been brought to the level of policy objectives and spread more widely to the public, existing academic research to understand its substantial and underlying implications has been insufficient. First, in this study, the characteristics of 479 'Unicorn Club' companies in 2019 were analyzed in-depth. Previous research has focused on the general status and trend by analyzing the number of unicorn companies by country and industry classifications. However, this study conducted a qualitative exploratory analysis by investigating descriptive statistics about unicorn companies, including their investors, while providing case studies. Also, cluster analysis, ANOVA, and multi-level regression were employed for quantitative exploration. The characteristics of individual companies were examined based on the "ERIS Model (Entrepreneur - Industry(Market) - Resource - Strategy Model)". Secondly, factors influencing its valuations were examined in connection with the previously analyzed characteristic variables and investor characteristics. Finally, based on these, the future direction of the "Unicorn Phenomenon" from the perspective of "Enterprise Ecosystem" and productively using it from the perspective of the public policy is suggested.