• Title/Summary/Keyword: 면화

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Development of simultaneous detection method for living modified cotton varieties MON757, MON88702, COT67B, and GHB811 (유전자변형 면화 MON757, MON88702, COT67B, GHB811의 동시검출법 개발)

  • Il Ryong Kim;Min-A Seol;A-Mi Yoon;Jung Ro Lee;Wonkyun Choi
    • Korean Journal of Environmental Biology
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    • v.39 no.4
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    • pp.415-422
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    • 2021
  • Cotton is an important fiber crop, and its seeds are used as feed for dairy cattle. Crop biotechnology has been used to improve agronomic traits and quality in the agricultural industry. The frequent unintentional release of LM cotton into the environment in South Korea is attributed to the increased application of living modified (LM) cotton in food, feed, and processing industries. To identify and monitor the LM cotton, a method for detecting the approved LM cotton in South Korea is required. In this study, we developed a method for the simultaneous detection of four LM cotton varieties, MON757, MON88702, COT67B, and GHB811. The genetic information of each LM event was obtained from the European Commission-Joint Research Centre and Animal and Plant Quarantine Agency. We designed event-specific primers to develop a multiplex PCR method for LM cotton and confirmed the specific amplification. Using specificity assay, random reference material(RM) mixture analysis and limit of detection(LOD), we verified the accuracy and specificity of the multiplex PCR method. Our results demonstrate that the method enabled the detection of each event and validation of the specificity using other LM RMs. The efficiency of multiplex PCR was further verified using a random RM mixture. Based on the LOD, the method identified 25 ng of template DNA in a single reaction. In summary, we developed a multiplex PCR method for simultaneous detection of four LM cotton varieties, for possible application in LM volunteer analysis.

An Ecological Reflection on the Food Self-Sufficiency Debate of the Antebellum American South (남북전쟁 이전 미국 남부지방 식량자급 논쟁의 환경사적 검토)

  • Keumsoo Hong
    • Journal of the Korean Geographical Society
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    • v.39 no.2
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    • pp.171-194
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    • 2004
  • The antebellum American South has been characterized by the lingering backward images of plantation, slaves and cotton. The South specializing in the cotton cultivation is compared with the manufacturing East and the breadbasket Midwest. Douglass North who examined the interregional trade assumed that the South up until 1860 relied on the Midwest for the foodstuffs. Statistical and literary evidence, however, disputes the North's model, showing instead that the southern region attained self-sufficiency in foodstuffs at least in the late 1830s or early 1840s. The South's food self-sufficiency is attributable, to a greater extent, to the region-wide environmental movement of scientific agriculture launched to address the aggravating soil problems from cotton monoculture. Diversification and crop rotation lied in the center of the new regime. The new agricultural system combining com, cotton and cowpea ensured the procurement of hoecake, hog meat, and cotton. The most significant outcome of the good farming regime, however, was the enhanced environmental consciousness which came to prevail the best farmer's reckless rush for profit maximization.

The Effects of Ethre1 Spraying on Shortening Maturity, Yield of Seed Cotton before Frost and Fiber Quality of Upland Cotton (Ethrel처리가 맥후작 면화의 숙기단축과 수량 및 섬유품질에 미치는 영향)

  • Jung-Il Lee;Eung-Ryong Son;Dal-Ho Choi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.20
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    • pp.115-121
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    • 1975
  • To achieve self-sufficiency in good production, cotton should be grown after barley in the southern part of Korea. But, this cropping system reduces cotton yields and fiber qualities in the current leading cotton varieties because of the short frost-free growth period. Ethrel-treated plots shortened the time to open boll by about 20days, increased the percentage of open bolls before the first frost from 38% to 93% and increased yield by 15-38%. There are not significant differences in staple length, tensile strength of the fiber, single bollweight, 100 seeds weight and germination percentage of the seeds between Ethrel-treated and untreated plots. Ethrel should be recommended to the farmers growing cotton after barley harvest.

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Multiplex PCR method for environmental monitoring of approved LM cotton events in Korea (국내 승인 LM면화의 자연환경 모니터링을 위한 multiplex PCR 개발)

  • Jo, Beom-Ho;Seol, Min-A;Shin, Su Young;Kim, Il Ryong;Choi, Wonkyun;Eum, Soon-Jae;Song, Hae-Ryong;Lee, Jung Ro
    • Journal of Plant Biotechnology
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    • v.43 no.1
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    • pp.91-98
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    • 2016
  • The growth area of living modified (LM) cotton has steadily increased every year, since its first commercialization in 1996. Development of environmental risk assessment tools and techniques for LM cotton is required for ecosystem safety. We therefore developed multiplex PCR assays for simultaneous detection of two (MON15985, MON531) and four (GHB614, LLCOTTON25, MON88913 and MON1445) LM cotton events approved in Korea, with event specific primer pairs. The PCR reactions were optimized by using event specific primers of six LM cottons at various concentrations. The reactions allows amplification of estimated amplicons of MON15985 (214 bp), MON531 (270 bp), GHB614 (119 bp), LLCOTTON25 (164 bp), MON88913 (276 bp), and MON1445 (389 bp) from multiplex PCR reactions. The multiplex PCR assay developed allowed that two annealing steps (15 cycles at $55^{\circ}C$ and 25 cycles at $60^{\circ}C$) were performed for amplification of distinguished two LM cottons, and only one annealing step (50 cycles at $60^{\circ}C$) was necessary for tetraplex PCR. Primer extension step of all PCR reactions was skipped for time-effective amplification. Our methods suggest that two multiplex PCR assays can be cost-effective and a rapid diagnostic tool for environmental LMO monitoring of six LM cottons.

LCD 대화면화에 따른 CCFL의 기술개발 방향

  • Kim, Jung-Hyeon;Hwang, In-Seon
    • The Proceedings of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.19 no.1
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    • pp.15-21
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    • 2005
  • 이제까지 주로 LCD가 대화면화가 진행될 때 CCFL의 기술 개발 동향을 살펴 보았다. CCFL lamp는 LCD module의 BLU에서 광원으로 중요한 자리를 차지해 왔고, 원가 절감 및 성능향상이라는 목표로 지속적으로 기술 개발이 되어 왔다. 하지만 현재 LCD TV가 FPD 시장의 주도적인 역할을 하기 위해서 혁신적인 기술 개발이 요구되어서 EEFL, FFL, LED 광원, CNT 광원 등의 다양한 형태의 광원이 CCFL의 자리를 넘보고 있는 실정이다. 이에 CCFL 메이커들 또한 다른 광원들의 장점을 CCFL 내에서 구현하고, 자신만이 가지고 있는 기술로 주력광원으로서의 자리를 잃지 않기 위해 기술 개발이 더욱 빠르게 이루어져 가고 있다.

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A Study on the Ignition Behaviors of Textiles according to Permeation Amount of Oils and Aeration (유지류의 침윤량과 공기주입에 따른 면화류의 발화거동에 관한 연구)

  • 오치훈;이창우;김정환;현성호
    • Fire Science and Engineering
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    • v.14 no.1
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    • pp.8-12
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    • 2000
  • We had investigated thermal and ignition behaviors of textiles. Decomposition of textiles with temperature was investigated using a DSC and the weight loss according to temperature using a TGA in order to find the thermal hazard of textiles, and the ignition behaviors of textiles according to species and permeation amount of oil. In addition, ignition behaviors of those permeated into oils indicating different iodine value and of those with arid without air in reaction vessel of measuring equipment were studied with constant temperature method among ignition temperature measuring methods. As results, the range of decomposition temperature of synthetic fiber was slightly broad compared with that of natural fiber, pure cotton. Besides, the initiation temperature of heat generation of both samples riced in the case of no air injection in the reaction vessel. On the other hand, in the case of air injection that was lowered according to the increase in permeative amount of oils and fats and decreased quickly as sample was permeated into drying oil.

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A study on the process of spatial reduction of cotton culture in Korea since 1945 (해방 이후 우리나라 면작농업 소멸의 지역적 전개과정)

  • ;Kim, Kihyuk
    • Journal of the Korean Geographical Society
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    • v.29 no.3
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    • pp.318-339
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    • 1994
  • U.S. had given large amount of cotton to Korea as food aid program since 1945. This cotton aid had negative impact on cotton culture in Korean agriculture. Korean government used counterparts funds (sale proceeds of food aid) not for investment to agriculture sector, but for military budgets. And food aid on program type had influenced general economic policies, which neglected agricultural sector too. Anti-agricultural policy which was helped by U.S. food aid, had caused cotton cultivator an economic loss. So this economic loss had made many farmers abandon cotton culture. But in our times, cotton is cultivated for the purpose of domestic consumption in a few rural villages. The purposes of this study are 1) to analyze the process of spatial reduction of cotton culture since 1945 in regional contexts in Korea, and 2) to identify the function and meaning of cotton culture which does not pay off in agricultural region. Materials for acreage of cotton culture are acquired through the agricultural statistical year book(1952-1989) and census. To clarify the meanings of cotton culture, field survey are conducted in a rural village which is identified as only one where cotton was cultivated in 1993. In these contexts, this study has come to the following conclusions. In the period of under the rule of Japanese Imperialism (1910-1945), G. arboreum, species of cotton which was traditionally cultivated since 1364, had been driven out. And G. hirustun species, which is suitable for the production of highly qualified textile, has been hierarchically diffused by policy. In these period, regional structure of Korean agriculture was reorganized for the provision with food to Japan. Crops leading this dependent spatial structure were rice and cotton. So agricultural region, specialized with cotton, were distributed in the hinterland of the area which is specialized with rice. U.S. cotton aid to Korea began in 1947. U.S. took an interest in agricultural export because of her domestic surplus of cotton. Cotton aid is one mechanism by which U.S government developed agricultural market in recipient countries, Specially in the exchange rates, up-valuation of won to the U.S. dollars made domestic cotton more expensive than cotton imported, Production cost of domestic cotton is higher than Government's purchasing price of cotton which was also more expensive than price of cotton imported. Korean farmer could not help abandoning the cultivation of cotton, and this gave rise to spatial reduction of cotton culture. Spatially, cotton culture was abandoned in early stage of reduction in regions where stand at a disadvantage climatically, and in next stage in regions where other up-land crops which paid off in urban market, eg, fruits, could be cultivated. In the stage of extinction, cotton was cultivated only in area where G. hirustun species was originated in Korean peninsula. This region is not only suitable climatically for cotton culture, but is far away from urban market. Use of cotton produced is not for spinning, but for fillings of comforter. The main purpose of cotton culture in rural village is not for cotton yields, but for increase of production of seasame, which is grown together with cotton as mixed crops. Cotton product are used for domestic consumption and sold out to gin house. Though cotton culture is not paid off, farmer wanted to cultivate continuously for the cultural purpose, and they wanted the cotton culture promotion policy with the goverment subsidy.

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비정상상태에서의 미생물과 과도응답

  • 조영일;김우식
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1975.07a
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    • pp.110.3-110
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    • 1975
  • 환경인자로써 배양액의 희석속도와 기본기질의 농도를 변화시킨 과도상태에서의 yeast의 대사기능의 면화를 고찰하고 과도응답을 해석하였다. 그 결과 기질의 잔존농도가 충분할때는 응답이 늦었으며 또 희석속도가 클수록 새로운 정상상태에 도달하는 시간이 길었다.

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Effects of Decreasing Methods of Salt Content in Root Zone on Soil Properties and Crop Grwoth at the Newly Reclaimed Tidal Soil (신간척지(新干拓地)에서 근권(根圈)의 염농도(鹽濃度) 저하(低下) 방법(方法)이 토양특성(土壤特性)과 작물생육(作物生育)에 미치는 영향(影響))

  • Cho, Yeong-Kil;Jo, In-Sang;Um, Ki-Tae
    • Korean Journal of Soil Science and Fertilizer
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    • v.25 no.2
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    • pp.127-132
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    • 1992
  • This experiment was conducted to find out the useful data for upland crop cultivation in the newly reclaimed tidal land. Poseung silty clay loam soil was selected, and cotton(Mogpo VII) and tall fescue were cultivated under different drainage systems and soil ameliorator applications. Soil hardness and bulk density were decreased by subsurface drainage and plastic film installed at 40cm depth of the soil. Red earth application was also effective to loosen the soil, but zeolite and gypsum made the subsoil compact. Water content of the soil was high in surface drain than that of subsurface drain or plastic film curtain plot during dry season. The water content was in order of plastic film curtain, surface drain and subsurface drain. Electrical conductivity(EC) was decreased to lower than 0.4 Simens $meter^{-1}$ ($SM^{-1}$) in the subsurface drain during rainy season, and the EC of subsurface drain was maintained a quater to an half of surface drain. The yield of cotton and tall fescue were high in order of subsurface drain, plastic film curtain and surface drain plot. The yields of cotton were increased to 36-73 % by ameliorator application, and the red earth application was more effective for tall fescue growth compare to gypsum and zeolite.

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