• Microbiology and Biotechnology Letters
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• v.30 no.3
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• pp.210-215
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• 2002

A bacterium degrading soy protein was isolated from Korean traditional fermented foods. The isolated strain was identified as Bacillus subtilis, and named as B. subtilis EB464. The optimum pH and temperature of the protease produced by 5. subtilis EB464 were pH 9.0 and $50^{\circ}C$, respectively. The protease was stable in the range of pH 6~10 and below $40^{\circ}C$. The content of water-soluble protein and free amino acid of the medium were increased from 4.2% to 20.6% and ken 1.9% to 22.0%, respectively, by solid-state fermentation of soybean meal with B. subtilis EB464 for 72 h.

• The Journal of the Korea Contents Association
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• v.7 no.11
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• pp.9-22
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• 2007

While standardization and its applications for registering and sharing information resources about B2B transactions such as business documents are relatively well developed, it is not easy to register and share business process resources because there are many ways to define complex business processes using different specification(definition) languages. In practice, there are several competing business process specification languages applicable under service-oriented architecture (SOA) such as ebXML BPSS, WS-BPEL, BPMN and so on. A systematic way has to be prepared to register/share diverse and heterogeneous specifications represented using those languages. This paper demonstrates the usefulness of sharing B2B business processes by prototyping a business process registry called ebRR4BP. First of all, we designed a metadata ontology to support the registration of diverse B2B business processes. To implement the proposed metadata ontology using ebXML registries, a mapping scheme to ebXML Registry Information Model is also suggested. The ontology and mapping scheme will be a foundation for supporting common interchange of business process metadata among B2B resistries.

• Journal of Fisheries and Marine Sciences Education
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• v.28 no.4
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• pp.903-912
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• 2016

Hagfish which belongs to the chordate contact cyclostomata, is important phylogenetic relationship between vertebrate and invertebrate. Cathepsins of the cysteine protease family have traditionally been thought to play a major role in intracellular protein degradation and turnover in lysosomes. In this study, Catepsin L was cloned from Inshore hagfish (Eptatretus burgeri), the cDNA encoding ORF of the Eptatretus burgeri Cathepsin L (EbCtL) is 978 bp. The cDNA encoding proEbCtL was expressed in Escherichia coli strain BL21(DE3) using the pGEX-4T-1 expression vector system. The recombinant proEbCtL protein was overexpressed as a approximately 55 kDa fusion protein. The overproduced soluble GST-fusion protein was then applied to glutathione-Sepharose 4B column chromatography; the sample harboring the fusion protein evidenced a high degree of purity when analyzed via SDS-PAGE and Western blot analysis. Its activity was quantied by cleaving the synthetic peptide Z-FR-AMC, Z-LLE-AMC, and Suc-AAF-AMC, and the optimal pH for the protease activity was 8, 9.5, and 9, respectively.

• Journal of Internet Computing and Services
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• v.3 no.4
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• pp.1-9
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• 2002

As a XML Schema has been settled to W3C as standard at May. 2001 and technologies like as XML. Xpath and NameSpace related with XML has been developing. XML is becoming the standard of the new document exchanging. Due to the ability expressing various types of documentstructure through XML, ebXML, RosettaNet, and BizTalk are using XML as a core technology in the part of e-Business, To make the task automatically in the B2B EC now, a standard XML document is needed. Internal documents in each company should be transformed differently without any loss to work with other companies. In this paper, transforming XML document based on JAVA servlet is considered as realization of a XML system to exchange documents safely.

• The Journal of Society for e-Business Studies
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• v.9 no.4
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• pp.53-64
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• 2004

Today's enterprises take processes beyond their own organizational boundaries in order to electronically trade goods and services with partners under the concept of B2B e-commerce. In this environment, inter-organizational business processes are required and should be well defined not only in public processes between partners but in private processes within individual partners. For this purpose, we propose the method to represent inter-organizational business processes. First of all, a feasible modeling method for the inter-organizational workflow for B2B e-commerce is developed. This method is proposed based on BPSS in ebXML so that the binary and multiparty collaborations share a common process. In this method, message flows and control flows are separated in order to facilitate the design procedure of the inter-organizational workflow process. Second, a well-structured process modeling algorithm to design a well-structured inter-organizational workflow process is proposed. In the algorithm, a process is transformed to a Petri-net-based process model. This algorithm employs well-behaved modeling blocks, well-behaved control structures, and business transactions to develop well-structured process models by a top-down design.

• Bulletin of the Korean Chemical Society
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• v.29 no.3
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• pp.615-619
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• 2008

Eight triterpenoids, six lanostanes 1-6, one lupenane 7, and one oleanane 8, were isolated by bioactivity-guided fractionation of the ethylacetate extract from roots of Glycine max (L.) Merr. All isolated compounds were examined for their inhibitory activities against human ACAT-1 (hACAT-1) and human ACAT-2 (hACAT-2). Among them, three triterpenoids showed potent hACAT inhibitory activities, (24R)-ethylcholest-5-ene-3,7-diol (1) and 3b -hydroxylup-20(29)-en-28-oic acid (7) exhibited more potent inhibitory activity against hACAT-1 (1: IC50 = 25.0 1.2 and 7: IC50 = 11.5 0.4 m M) than hACAT-2 (1: IC50 = 102.0 5.4 and 7: IC50 = 33.9 3.7 m M), respectively. Interestingly, 5a ,8a -epidioxy-24(R)-methylcholesta-6,22-diene-3b -ol (4) has proven to be a specific inhibitor against hACAT-1 (IC50 = 38.7 0.8 m M) compared to hACAT-2 (IC50 >200). In conclusion, this is the first study to demonstrate that triterpenoids of G. max have potent inhibitory activities against hACAT-1 and hACAT-2.

• Journal of Trauma and Injury
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• v.30 no.4
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• pp.145-157
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• 2017

Purpose: We accessed epidemioloy of 908 acute burns (7 years) in the military, of injuries and propose proper educational programs to suit community. Methods: We surveyed burn demographics, circumstances of injuries, size, result of treatment. Results: The mean age was 20.6 years. The flame burns (FB) (325, 35.8%) were most common, followed scald (SB) (305, 33.6%), contact (CB) (219, 24.1%), electric (EB) (45, 5.0%) and chemical burns (ChB) (14, 1.5%). The more occurred during winter (29.7%). SB had mean 3.9% total body surface area (TBSA). The 251 (82.3%) had superficial burns by spillage of hot water/food on lower limbs (45.6%), feet (33.8%) in summer (34.8%), treated with simple dressing (92.8%). Morbidity rate was 5.6%; post traumatic stress disease (PTSD) (0.7%). FB had large wound (9.3% TBSA). The 209 (64.3%) had superficial burns by ignition to flammable oils (31.7%) and bomb powders (29.2%) on head/neck (60.3%), hands (58.6%) in summer (31.7%), autumn (30.2%). They underwent simple dressing (83.4%) and skin graft (16.0%). Morbidity rate was 18.8%; PTSD (10.5%), inhalation injuries (4.0%), corneal injury (3.7%), amputations (0.9%), and mortality rate (1.2%). CB had small (1.1% TBSA), deep burns (78.5%) by hotpack (80.4%) on lower limbs (80.4%). The more (59.8%) underwent skin graft. EB had 6.8% TBSA. The 29 (64.4%) had superficial burns by touching to high tension cable (71.1%) on hand (71.1%), upper limbs (24.4%) in autumn (46.8%). They underwent simple dressing (71.1%) and skin graft (24.4%). They showed high morbidity rate (40.0%); loss of consciousness (13.3%), nerve injuries (11.1%), neuropathy (8.9%), amputations (2.2%), and mortality rate (2.2%). Conclusions: The cook should wear apron over the boots during work. The lighter or smoking should be strictly prohibited during work with flammable liquids or bomb powders. Don't directly apply hotpack to skin for a long time. Use insulating glove during electric work. Keep to the basic can prevent severe injury and proper education is important.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.14 no.12
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• pp.1300-1310
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• 2003

In this paper, the performance of a multicarrier CDMA system using adaptive modulation and adaptive subchannel allocation scheme is analyzed in Rayleigh fading channel. The proposed adaptive modulator consists of modulation schemes using QPSK 16 QAM, 64 QAM and 256 QAM and constellations are pointed by Gray code. In addition, the threshold of the analysis is average E$\_$b//N$\_$o/ when the BER is 1 %. In the multicarrier system with adaptive subchannel allocation scheme, each DS waveform of user is transmitted over the K subchannels with the biggest fading among L subchannels. In case of the proposed system, total 4 subchannels are used and data are transmitted over 2 subchannels with the biggest fading, which results in the threshold of each channels is 5.2 dB, 9 dB, 13.2 dB and 8.4 dB, 12.2 dB, 16.3 dB. In the case of proposed system, the BER of 10$\^$-3/ is satisfied if average E$\_$b//N$\_$o/ is 8.1 dB. This is increased performance of 12.9 dB in comparison with conventional system. The BPS according to average channel is needed average E$\_$b//N$\_$o/ of about 15 dB in 7 bit. In the case of subchannel error, the BER of 10$\^$-3/ is 13.6 dB and is declined about 5.5 dB.

• Journal of Embryo Transfer
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• v.16 no.3
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• pp.193-201
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• 2001

This study was designed to examine the factors affecting in fertilization and development of embryos in vitro, and to examine whether zone drilling by laser irradiation can improve the hatching rate of IVF embryos from DNA marker-proved Hanwoo. DNA markers related to marbling score were identified using DNA fingerprinting with Ml3 probe and restriction enzyme Hae III. Oocytes were aspirated from immature ovarian follicles using a combined method of rectal ovarian-palpation and transvaginal ultrasound-guidance(6.5MHz) under local anesthesia. The aspirated oocytes were washed twice with fresh D-PBS containing 5% FBS and were rewashed 4 to 5 times with TCM-199 containing 5% FBS. A morphological grade of I to IV was assigned to each oocyte. Data were analyzed using the GLM procedure of SAS. Sperm separation methods did not have any significant effect on cleavage or developmental abilities of IVF embryos. Significantly(P<0.05) higher cleavage rate was observed in embryos from GI(60.0%, 3/5), GII(69.2%, 18/26) and GIII(62.1%, 59/95) compared to embryos from GIV oocytes(36.2%, 25/69). And the developmental rate to blastocyst stage was higher(P<0.05) in embryos from GI(33.3%, 1/3) and GII oocytes(38.9%, 7/18) than those from GIII(16.9%,10/59) and GIV oocytes(4.0%, 1/25). There was no significant difference in development of IVF embryos to blastocyst by media for in vitro culture. Proportion of hatched blastocyst was significantly(P<0.05) higher in embryos received zona drilling by laser than those of non-drilled.

• Journal of the Institute of Electronics and Information Engineers
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• v.53 no.9
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• pp.67-74
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• 2016

This paper presents a novel multiple orthogonal subcarrier modulation based UHF band RFID communication system. In tag-to-reader communication, the demonstration system can deliver 1.6 Mbps through four subcarriers. To improve data rate while suppressing increase in circuit complexity, tag employs square-waves as the subcarriers and uses individual load modulators for each subcarrier. By using multiple orthogonal subcarrier based modulation, proposed communication system can be operated under existing UHF band RFID regulation. In reader, an OFDM demodulator is used. Since the tag backscatters the reader's CW carrier, carrier frequency offset compensation is not necessary in reader demodulator. Experimental results show that the demonstration system achieves a bit error rate of 10-5 at an Eb/N0 of 10.8 dB.