• Title/Summary/Keyword: 메기

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메기 Silurus asotus 발생난의 핵분열

  • 임재현;박인석;허준욱;정지혜;김동수
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2003.10a
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    • pp.30-30
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    • 2003
  • 자성발생(gynogenesis)은 대상 어류의 성 결정기작이 암컷 동형접합성(female homogamety)인 경우 전 암컷 단성집단을 생산할 수 있으며, 단기간 내에 우수한 품종의 순계 획득과 유전적으로 동일한 clone 집단을 만들 수 있다(Thorgaard, 1986; 정 등 1996). 이에 제 1 난할 억제에 의한 메기 S. asotus에서의 체세포분열 억제성(mitotic) 자성발생 2배체가 유도된 바 있다(임 등, 2002), 염색체공학 기법 중 웅성발생 2배체(androgentic diploid)는 난자 핵을 불활성 시킨 후 정자 핵에 의해 제 1 난할억제에 의한 유전자 배가로 유도 될 수 있다. 본 연구는 염색체공학에 의한 메기 4배체 생산, 메기체세포분열 억제성 자성발생성 2배체의 효과적인 유도 조건 검정 및 메기 웅성발생성 2배체 생산을 위한 연구의 일환으로, 메기에서의 핵분열(korykinesis)에 관하여 조사하였다. 메기에서의 핵분열 수온 25$\pm$0.5$^{\circ}C$ 조건에서 수정 후 31분에 가장 현저하였다. 본 연구 결과를 Park and Im (2001)의 수온 24$^{\circ}C$에서의 제 1 난할 후기가 50분이며 mitotic interval이 18.5$\pm$1.2분인 결과와 비교시, 본 연구 결과의 수온 24$\pm$0.5$^{\circ}C$ 조건에서 핵분열이 수정 후 31분인 것은 핵분열과 세포질분열(cytokinesis)이라는 관점에서 정확히 일치한다. 염색체 수의 배가시, 핵분열 억제는 세포질 분열 억제에 비해 더욱 효과적으로서(Cherfas et al., 1993; Nam et al., 1999), 본 연구 결과의 핵분열 시간(수온 24$^{\circ}C$ 조건에서의 수정후 31분)을 기반으로 하여 차후, 4배체 메기와 체세포분열 억제성 자성발생 2배체 메기 및 웅성발생성 2배체 메기 유도 및 그의 생산성 향상에 관한 연구가 기대된다.

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Histological Study of Oculocutaneous Albinism in Korean Far Eastern Catfish Silurus asotus (백색증 메기 Silurus asotus에 관한 조직학적 연구)

  • Park, Jong-Young;Oh, Min-Ki;Yoon, Seung-Woon;Lee, Wan-Ok
    • Korean Journal of Ichthyology
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    • v.21 no.2
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    • pp.100-105
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    • 2009
  • Histological study of normal and albinic catfish Silurus asotus produced in aquaculture has been carried out on organs such as the eye, the barbel, the dorsal fin, and the skin (including dorsal, ventral and lateral regions). Although individuals have no differences in morphological appearance, their colors showed clear difference as follows: the normal catfishes were black overall, but the albinic ones have a yellowish-white color over the whole body, red eyes and white barbels. All the organs investigated were fundamentally very similar in structure. Regarding the distribution and density of the melanin pigments, however, the albinic catfish have no melanin in the skin and eye, particularly in the basement membrane of the epidermis and the pigment epithelial layer in the retina, while the normal catfish have lots of melanin. This phenomenon may coincide with the so-called $i^1/i^1$ type of oculocutaneous albinism.

Comparision of Carotenoid Pigments in Catfish, Parasilurus asotus and Slender catfish, Parasilurus microdorsalis in the Family Siluridae (메기과에 속하는 메기와 미유기의 Carotenoid 색소 성분의 비교)

  • 백승한;하봉석;김수영;권문정;최옥수;배태진;강동수
    • The Korean Journal of Food And Nutrition
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    • v.17 no.2
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    • pp.156-162
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    • 2004
  • This study was performed as a part of comparative biological studies of carotenoid pigment for the unutilized biological products. The content of total carotenoid in the integument of wild catfish were 0.27(${\pm}$0.7) mg% and composed of 25.8% zeaxanthin, 9.7% diatoxanthin and 9.1 % cynthiaxanthin as major carotenoid. The content of total carotenoid in the integument of cultured catfish were 0.07(${\pm}$0.03) mg% which is relatively lower compare to wild catfish and composed of 48.5% lutein, 13.9% zeaxanthin and 13.3% isocryptoxanthin as major carotenoid. The total carotenoid contents of the slender catfish were 0.75(${\pm}$0.25) mg% which is relatively higher compare to other species of catfishes. The carotenoids were composed of 24.5% zeaxanthin, 24.1 % 7'8'-dihydro-${\beta}$-carotene-4-ol, 17.9% 7'8'-dihydro-${\beta}$-carotene and 10.8% 7'8'-dihydro-${\beta}$-carotene-3-01 as major carotenoid and 8.7% diatoxanthin, 6.7% cynthiaxanthin and 5.0% lutein as minor carotenoid. Based on these data, as a comparative studies of carotenoid in integument of siluridae, parasiloxanthin and 7',8'-dihydroparasiloxanthin which are the characteristic carotenoid of catfish from biwa lake in Japan, Slender catfish contained more based on 7',8'-dihydro-${\beta}$-carotene while that of wild and cultured catfishes were not found, indicating that carotenoid pigment of slender catfish depend on their living conditions.

The Study of the Kwamaegi Preference in Pohang (포항지역 주민의 꽁치 과메기 기호도 조사)

  • 조영대;김정애;오승희
    • The Korean Journal of Food And Nutrition
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    • v.13 no.3
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    • pp.255-262
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    • 2000
  • 포항지역 주민 500명을 대상으로 꽁치과메기의 기호도 조사를 한 결과를 요약하면 다음과 같다.과메게 용어 인지 여부에 대해서는 대부분 응답자가 알고 있었으며, 과메게 시식 여부는 92.5%가 먹어 보았으며, 먹어보 지 못한 7.5%에 대해 그 이유를 알아본 결과 먹고 싶지 않았다가 61.3%를 차지하였다. 연령별 시식 여부 차이 분석에서 나이가 많을수록 대부분 과메게를 먹어본 것으로 나타났다.(P<0.05). 과메기를 먹는때는 술안주로가 80.9%로 가장 많았다. 남(86.5%), 여(56.8%)모두 술안주로 먹는 경우가 가장 많았다. 간식으로 먹는 경우도 남자(6.6%)보다 여자(16.3%)가 많게 나타났다(P<0.05). 또한 연령이 많을수록 간식으로 먹는 경우가 많았다.이러한 결과를 놓고 볼때 사철과메기의 개발 또는 포항 꽁치과메기가 향통식품으로 자리잡기 위해서는 술안주로서의 과메기가 아니라 조리방법이나 스낵 등의 가공방법 개발 등을 통하여 반찬이나 간식으로 먹을 수 있어야 진정한 먹거리로서 자리잡을 수 있을 것이다. 과메기를 처음 먹은 꼐기는 주위의 권유로 먹은 경우가 57.9%로 가장 많았다. 연령별로 살펴보면 젊을수록 과메기를 주위의 권유로 먹은 경우가 많으며, 나이가 많을수록 먹음직스러워서 과메기를 처음 먹은 계기가 된것으로 나타났다.(P<0.05). 과메기 선호도는 92.5%가 과메기에 대해 호의적인 응답을 하였다. 남자가 여자보다 과메기를 더 선화하는것으로 나타났다.(P<0.05) 연령별로는 31~40세 연령층이 과메게를 가장 선호하는 계층으로 나타났다. 과메게를 먹을때 곁들여 먹는 것으로 과메기, 생미역,초고추장으로 먹는 경우가 가장 많았다. 포항 지역 주민들은 과메기를 먹을때 대부분 생미역과 곁들여 먹지만 초고추장 없이 먹는 경우도 적지 않았다. 대부분 3~10마리 정도 1회 먹는것으로 나타났으며, 20마리 이상 많이 먹는 주민도 남녀 거의 유사하게 나타났다. 따라서 여성층의 과메기 애호가도 많다는 것(5.6%)을 알 수 있다.(P<0.05)

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Antibacterial effects of Chitosanon-ascorbate Treated Kwamaegi Prepared on Microorganism Contamination (Chitosan-ascorbate 처리 과메기에 있어서 오염미생물에 대한 저해효과)

  • Kim, Young-Sook;oh, Seung-Hee;Kim, Soon-Dong
    • KSBB Journal
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    • v.24 no.2
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    • pp.156-162
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    • 2009
  • We examined saury, herring, gizzard shad kwamaegi to measure of microbic contamination rate of kwamegi that are sold in the market now. In the total bacteria, staphylococcus, peroxide value, and microorganisms is inhibited that from sample that we treated a substance with chitosan-ascorbate (CA) and other orders deep water (DW), ginseng steamed red and wine (GRW), NT (not treated). When we compared between SGRW and SNT, SCA show us more inhibition effect 0.22-0.49 log cycle in the total aerobacter. When we compared between HDW and HNT, HCA restraint 0.05-0.45log cycle, and when we compared between GDW and GNT, GCA inhibited 0.45 log cycle. In the coliform and E. coli, growths of microorganisms were inhibited followed order by treatment of CA, NT, and DW. GDW, HCA and HNT checked enough amount of water from the moisture measurement; but SGW, GCA, HEW and SCA showed 7-15% lack of moisture, and SNT and GNT have 10% more moisture. Peroxide value is changed to 41-51meq/kg when we did treat CA in there and a side that didn't add antimicrobial expressed the result numerically that 56-58meq/kg. In the sensory evaluation, customer gave preference to followed by Saury kwamaegi, herring, and gizzard shad kwamaegi. We have a point of view when kwamaeki manufactured if we add natural antibiotic and it uses to vacuum drying, we would inhibited of multiplication of microorganism, and of peroxides.

Characteristics of the Histological Structure of the Mandibular Barbels of Two Species of Catfish (Siluridae) from Korea (한국의 메기과 (Siluridae) 어류 2종의 상악 수염의 조직학적 특성)

  • Park, In-Seok;Kim, Chi-Hong
    • Korean Journal of Ichthyology
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    • v.17 no.1
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    • pp.36-42
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    • 2005
  • Histological observations on the barbels of catfish (Siluridae) in Korea are reported. The mandibular barbel of Far Eastern catfish, Silurus asotus, has epidermis and dermis surrounding an axial rod of cartilage. The stratified epidermis embodies mucous cells, club cells, and taste buds. The mucous cells, which are thin rims of basophilic cytoplasm, and the taste buds, which are basophilic, are located at the surface and the distal portion of the epidermis, respectively. The basement membrane lies between the epidermis and dermis except in places of dermal papillae. The dermis is made up of loose connective tissue fibers enclosing nerve bundles and blood vessels. The central region of cartilage is innermost and enclosed within muscle layers. The mandibular barbel of slender catfish, S. microdorsalis, has an identical structure to those of the Far Eastern catfish however, the percentage thickness of epidermis in the upper and lower parts of the barbel and the number of taste buds in the upper and middle parts of the barbel are different from those of Far Eastern catfish. This study shows that the mandibular barbels in Far Eastern catfish and slender catfish are of a stiff and flexible type.

Studies on disease of catfish, Silurus asotus, in Korea. III. Edwardsiella ictaluri infection. (한국산 메기(Silurus asotus)의 질병에 관한 연구 III. Edwardsiella ictaluri 감염증)

  • Park, Sung-Woo;Kim, Young-Gill
    • Journal of fish pathology
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    • v.7 no.2
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    • pp.105-112
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    • 1994
  • A new bacterial infection occurred among the cultured Korean catfish, Silurus asotus, in Chunbuk prefecture, Korea, 1993. This disease produced about 30% mortality in the fish for 4 months. The diseased fish was swimming listless at the water surface with head up and tail down, sometimes spinnig in circles. The most outstanding clinical sign was ulceration on the skull and at the base of the pectoral fins. The causative organism was isolated from the brain, kidney, spleen and liver of diseased fish, and identified as Edwardsiella icaluri by the biochemical and biophysical characteristics. After intraperitoneal innoculaton of the isolate, the pathogenicity was proved positive for Korean catfish, S. asotus, and channel catfish, Ictalurus punctatus, but negative for carp, Cyprinus carpio.

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Optimum Protein Levels in Diet for Fingerling Korean Catfish, Parasilurus asotus (메기, Parasilurus asotus 사료의 최적 단백질 함량)

  • KANG Seok-Joong;JEONG Woo-Geon
    • Journal of Aquaculture
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    • v.6 no.1
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    • pp.47-53
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    • 1993
  • Feeding trials were conducted for a test of the optimum dietary protein levels for the fingerling Korean catfish, Parasilurus asotus. The growth response was examined in terms of weight gain, feed coefficient. protein efficiency ratio and net protein utilization for 8 weeks at $23\~27^{\circ}C$. Within a range of 25 to 55\%$ crude protein levels in the diet, the body weight increased while the dietary protein level increased. Accumulation of protein in the body reached the maximum when the crude protein level in the diet was at $45\%$. These results indicate that the optimum dietary protein level of fingerling Korean catfish was about $45\%$ when anchovy meal was used as the protein source.

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Spawning Behavior of the Silurid Catfish, Silurus asotus, Treated with Hormone in Laboratory Aquaria (호르몬 처리에 의한 수조내 메기 Silurus asotus 산란행동)

  • 최낙중;이완옥
    • Journal of Aquaculture
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    • v.10 no.1
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    • pp.63-68
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    • 1997
  • The spawning behavior of the silurid catfish, Silurus asotus, was observed from laboratory aquaria after treating with human chorionic gonadotropin (HCG) and injection with pituitary extract of grass carp. The details of the spawning behavior presented here are based upon the observations made from 10 trials with 40 male and 20 female catfishes artificially bred between May and July, 1995. Initial spawning activity started 15 to 16 hours after injection with pituitary extract of grass carp and HCG. The activity was continued with for 2 to 3 hours and repeated mating behavior of the catfish were observed during this period. Compared to the spawning activity of the Japanese catifish, S. biwaensis, S. asotus took a different male position during the period o fsearching its partner. This study also reports some other differences in mating behavior between S. asotus and S. biwaensis.

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