• 한국경제지리학회지
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• 제9권3호
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• pp.431-440
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• 2006

최근 세계적으로 지역경제의 활성화 정책에 문화예술활동을 도입하는 사례가 크게 늘어나고 있다. 일본의 경우에는 이미 수십, 수 백년 전부터 여러 지역에서 마을만들기 운동, 즉 "마치즈쿠리(まちづくり)"를 통해 지역 정체성을 강화하는 한편, 침체된 지방의 경제를 활성화하고 있다. 일본 시가현의 나가하마(長浜市)의 경우에는 1980년대 중반부터 마찌쯔꾸리운동을 통해 지역부존자원이 빈약한 인구 5만의 소도시가 전국적으로 알려지게 되었다. 나가하마는 문화활동의 활성화로 관광객을 유치하고, 지역 특산물을 홍보하여 지역경제의 활성화에 기여하는 한편, 그러한 성과에 기초하여 전문대학의 유치, 실내야구장의 건설 등과 같은 사업을 추진하여 지역주민들의 자긍심을 제고하는데 성공하였다.

• 한방안이비인후피부과학회지
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• 제27권3호
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• pp.115-132
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• 2014

Objective : Portulacae Herba (PH) can clear away heat, detoxicate, cool down blood heat. The present study was designed to investigate the effects of PH on ACD induced by 2,4-dinitrochlorobenzene (DNCB) in mice. Method : In this experiment, the effects of PH on changes in body weights, thicknesses and weights of ear, thicknesses of dorsum skin, degree of symptoms on the dorsum skin, histopathological changes of ear and dorsum skin, spleen weights, TNF-${\alpha}$ and IL-10 in serum were observed. And the effects on the proliferation rates of splenocytes were also investigated in vitro and in vivo. Results : 1. PH spread (PHS) group and PH spread plus administered (PHS+Adm) group didn't show any changes in weight and thickness of ear. 2. But in PHS and PHS+Adm group, thickness of dorsum skin decreased significantly. 3. And PHS and PHS+Adm group showed meaningful effectiveness on ACD symptoms like erythema, desquamation and keratinization. 4. In histopathological observation, hyperplasia and edema of spongy tissues were remarkably diminished in PHS and PHS+Adm group. 5. And PH reduced the proliferation rates of splenocytes in vivo and vitro study. Conclusions : PH effectively decreased symptoms of ACD in this study, therefore PH can be one of medicinal ingredients to treat ACD.

• Journal of Nutrition and Health
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• 제39권8호
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• pp.742-747
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• 2006

This study was performed to investigate the lipolytic effects of Portulaca oleracea L. extract in 3T3-L1 adipocytes. The Portulaca oleracea L. was extracted with extrusion method using twin-screw extruder under $58{\sim}60rpm$ screw speed, $4{\sim}5kg/hr$ feed rate, $140^{\circ}C$ extrusion temperature. The lipolytic action of Portulaca oleracea L. extract was estimated by measuring the amount of glycerol and free fatty acids (FFA) released from 3T3-L1 adipocytes and by measuring the cellular lipid content in 3T3-L1 adipocytes. The hormone sensitive lipase (HSL) mRNA level was analyzed using quantitative real-time PCR. The Portulaca oleracea L. extract at 1 to $100{\mu}g/ml$ suppressed lipid accumulation. The release of glycerol and FFA into the medium, and the mRNA level of HSL were significantly increased by the addition of Portulaca oleracea L. extract at dose-dependent manner. In conclusion, the Portulaca oleracea L. extract was suggested to have the lipolytic effect through release of lipolytic products (FFA and glycerol) of triacylglyceride to the culture medium and suppression of lipid accumulation via up-regulation of HSL gene expression in 3T3-L1 adipocytes.

• 대한본초학회지
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• 제31권1호
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• pp.61-67
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• 2016

Objectives : The present study aimed to investigate the anti-inflammatory effects of the water extracts of Portulacae Herba (PH).Methods : We measured the effects of the water extracts of Portulacae Herba (PH) on the cell viability of mouse macrophage RAW 264.7 cells, the intracellular calcium production, and the proinflammatory mediators including nitric oxide (NO), vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF)-BB, which are induced by the lipopolysaccharides (LPS), and obtained the results shown below.Results : After the cultivation of the PH extracts along with the mouse macrophages, the cell survival rate did not decrease with the MTT assay. However, the PH extracts did significantly suppress the production of NO by the mouse macrophages induced by LPS at the concentrations of 25, 50 and 100 ㎍/mL. The PH extract also significantly suppressed the VEGF, PDGF-BB and intracellular calcium production of the mouse macrophages by LPS at concentrations of 25, 50 and 100 ㎍/mL. As shown in the results above, the PH extracts do not have a toxic effect on the macrophages, but still have an anti-inflammatory effect that significantly reduces the intracellular calcium production as well as the production of NO, VEGF and PDGF-BB at concentrations above 25 ㎍/mL.Conclusions : In conclusion, the inhibitory anti-inflammatory effects of the PH extract can be used for a new treatment of anti-inflammatory diseases.

• 대한본초학회지
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• 제30권6호
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• pp.33-38
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• 2015

Objectives : Portulaca oleracea (PO) have been used as a traditional medicine to treat inflammatory diseases in Korea. However, the anti-inflammatory effect of PO ethanol extract on lipopolysaccharide (LPS)-induced inflammation is not well-known. Therefore, this study was performed to identify the anti-inflammatory effect of PO on LPS induced inflammatory.Methods : Identification of PO was conducted by comparison with purified standards by HPLC. To measure out the cytotoxicity of PO, author performed the MTT assay. To evaluate the anti-inflammatory effects of PO, author examined the inflammatory mediators such as nitric oxide (NO) and pro-inflammatory cytokines (tumor necrosis factor (TNF)-α, interleukin, (IL)-1β and IL-6) on RAW 264.7 cells. Author also examined molecular mechanisms such as mitogen-activated protein kinases (MAPKs) and nuclear factor-B (NF-κB) activation by western blot.Results : Three major components (peaks 1, 2, 3) were detected in both varieties and peak 1 was characterized as caffeic acid, peak 2 as p-coumaric acid, and peak 3 as ferulic acid by comparison of chromatographic properties with authentic standards. Extract from PO itself did not have any cytotoxic effect in RAW 264.7 cells. PO inhibited LPS-induced productions of inflammatory mediators such as NO and pro-inflammatory cytokines in RAW 264.7cells. In addition, PO inhibited the phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2), c-Jun NH₂-terminal kinase (JNK) and NF-κB activation in RAW 264.7 cells.Conclusions : Above experiment data can be an important indicator for the identification of PO and this study suggest that treatment of PO could reduce the LPS-induced inflammation. Thereby, PO could be used as a protective agent against inflammation.

• 대한한방부인과학회지
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• 제18권1호
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• pp.29-44
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• 2005

To address the ability of Herba Portulacae(HP) to induce cell death, we investigated the effect of HP on cell viability. Twenty-four hours later, loss of viability occurred following HP exposure in a dose-dependent manner. The treatment of HP, a commonly used herb formulation in Korea, Japan and China, caused a decrease in cell viability. HP also resulted in apoptotic morphology a brightly blue-fluorescent condensed nuclei by Hoechst 33258-staining, and reduction of cell volume. Our results show that 2mg/ml HP induces mitochondria membrane potential collapse. Immunoblotting data also shows that the expression of Bcl-2, antiaoptotic protein, decrease by the addition of HP. This GFP-Bax overexpression system shows that an important pro-apoptotic Bcl-2-family protein, Bax is translocated to mitochondria by the addition of 2mg/ml HP. Inerestingly, MAPK inhibitor study shows that p38 MAPK inhibitor, SB203580 inhibits HP-induced cell death and caspase-3 activation in HP-treated HeLa cells. Furthermore, HP transiently but significantly induces p38 activation. But P38 MAPK inhibitor does not have any effect on the translocation of Bax. Considering these results, HP induces apoptosis via p38 MAPK activation. But the pathway does not involve the translocation of Bax.

• 한방안이비인후피부과학회지
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• 제27권1호
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• pp.17-36
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• 2014

Objectives : The purpose of this study is to investigate the efficacy of external preparation containing herbal extract(Sophorae Radix, Aurantii Immaturus Fructus, Schizonepetae Spica, Forsythia fructus and Portulacea Herba) on atopic dermatitis patients. Methods : A total of 33 patients who visited the Dept. of Ophthalmology, Otolaryngology & Dermatology of Korean Medicine of Korean Medical hospital of Daejeon university from February 2012 to February 2013 were engaged in this study. Patients who corresponded the requirements were enrolled in the study. This study shows symptom comparisons before and after the use of this product. Effectiveness was evaluated through SCORAD(SCORing Atopic Dermatitis) Index, and the quality of life scale was conducted through Skindex-29. Results : 1. Comparison of the general changes through SCORAD Index indicated that the experimental group showed more statistically significant declines than the placebo group after the two-week use. 2. Comparison of changes in intensity criteria(Erythema, Edema, Oozing, Excoriation) through SCORAD Index indicated that the experimental group showed more statistically significant declines than the placebo group after the two-week use. 3. Comparison of changes in subjective symptoms through SCORAD Index indicated that the experimental group showed no statistically remarkable changes. 4. Comparison of changes in the body parts(Upperlimb) through SCORAD Index indicated the experimental group showed statistically significant changes after the two-week use. 5. Comparison of the general changes in quality of life through Skindex-29 indicated that the total score from experimental group showed statistically significant changes after the four-week use. Conclusions: This study, evaluated through SCORAD Index, indicated that the treatment with the external preparation containing herbal extract has a statistically marginal significance of efficacy on atopic dermatitis patients in the short term.

• 생약학회지
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• 제46권2호
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• pp.116-122
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• 2015

Keratinocytes are first barrier against outer challenges on skin. However, it is still largely unknown about effective protectors against ultraviolet B (UVB), and oxidative stress in human keratinocyte, HaCaT cells. Inducible heme oxygenase (HO)-1 acts against oxidants that are thought to play a role in the pathogenesis of skin disorders. Therefore, the purpose of this study was to evaluate the effect of Portulaca oleracea 70% EtOH extracts against hydrogen peroxide (H₂O₂)-induced oxidative stress in human keratinocytes, HaCaT cells. P. oleracea 70% EtOH extracts showed the potent protective effects on H₂O₂-induced toxicity by induced the expression of HO-1 in human keratinocyte, HaCaT cells. Furthermore, P. oleracea 70 % EtOH extracts caused the nuclear accumulation of nuclear factor E2-related factor 2 (Nrf2) in human keratinocytes, HaCaT cells. In addition, we found that treatment with c-Jun N-terminal kinase (JNK) inhibitor (SP600125) reduced P. oleracea 70% EtOH extracts-induced HO-1 expression, and JNK inhibitor (SP600125) also inhibited protective effects by P. oleracea 70% EtOH extracts. Therefore, these results suggest that P. oleracea 70 % EtOH extracts increases cellular resistance to H₂O₂-induced oxidative injury in human keratinocyte, HaCaT cells, presumably through JNK pathway-Nrf2-dependent HO-1 expression.

• 전기의세계
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• 제46권12호
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• pp.8-10
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• 1997

본 학회는 1947년 7월 9일 설립되어 금년으로 뜻깊은 창립 50주년을 맞이하였다. 학회에서는 1997년 7월 9일 창립 50주년 기념일을 맞아 창립 50주년 기념 각종 사업들을 진행하고자 지난 2월 이사회에서 50주년 기념사업 조직위원회를 구성하고 산하에 8개 분과위원회를 두었는데, 그중 50주년 기념행사중 제일 빅 이벤트인 창립 50주년 기념식과 축하연을 담당 진행할 목적으로 기획담당이사를 위원장으로 하여 기획분과위원회가 설치되었다. 기획분과위원회 위원으로는 전기학회 소속 교수중 업무추진력이 있는 중견교수로 구성되었다. 선임된 교수들은 준비하는 행사가 50년에 한번밖에 없다는 생각에 소명의식과 함께 가벼운 흥분을 느끼며 이와 같은 규모와 비중이 있는 행사개최에 대해 처음에는 걱정들이 앞섰으나, 선임된 위원들은 유사한 종류의 행사를 진행하고 준비한 경험이 있는 교수가 많아 행사준비가 원만히 진행될 수 있었다. 창립 50주년 기념식 행사는 아무런 차질없이 잘 진행된 것을 자랑스럽게 여기며 기념식 및 축하연 행사 내용 소개와 준비과정등 기록들을 남김으로서 향후 75주년, 100주년 기념사업을 준비하실 후학 여러분에게는 도움을 주고, 현 회원에게는 진행보고를 하고자 이 글을 남긴다.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2020년도 춘계학술발표대회
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• pp.417-419
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• 2020

데이터 3법 시대에 접어들면서 기업들에는 가명화된 개인정보를 활용할 수 있는 길이 열렸다. 하지만 현 데이터 3법은 데이터를 생성하고 유통하며 활용할 기업들의 책임과 혜택에 내용이 맞춰져 있어 아쉬운 감이 있다. 개인의 기본권을 보장하면서도 마이데이터 유통 및 활용을 도울 방법은 없을까? 본 논문에서는 데이터의 주체인 개인이 데이터 주권을 행사하고 실질적인 혜택을 받는 마이데이터 서비스의 활성화를 위한 ID 관리 기술로 블록체인 기반 분산 ID(Decentralized Identification, DID)를 제안하고, DID 수용의도에 영향을 마치는 요인을 연구함으로써 마이데이터 서비스 개발 활성화를 위한 정책적, 실무적 시사점을 도출하고자 한다.