• Title/Summary/Keyword: 마이크로그루브

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Manufacturing Technology of Lenticular Lens Mold by Shaping (세이핑에 의한 렌티큘러 렌즈 금형 가공)

  • Je T. J.;Choi D. S.;Lee E. S.;Shim Y. S.;Kim E. Z.;Na K. H.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2004.11a
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    • pp.249-254
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    • 2004
  • 광의 효율적 사용을 위해 표면에 마이크로 그루브가 새겨진 고성능 광학 부품의 개발이 활발하고, 이들 부품의 다량 생산을 위한 초정밀 금형제조기술이 각광을 받고 있다. 최근의 초정밀 미세 기계가공의 경우 간단한 공정으로 이러한 마이크로 그루브 금형을 제작할 수 있다. 특히 조명각 변조용 렌티큘러 렌즈와 같이 실린더형 그루브 금형의 경우에는 기존의 Lithography, MEMS, LIGA 등 광 에너지를 이용한 다른 제조방법들에서는 가공하기 어려운 점이 있으나, 기계가공에서는 쉽게 제작가능한 장점이 있다. 본 연구에서는 이러한 미세기계가공기술의 장점을 활용하여 U 형 마이크로 그루브를 가진 Lenticular 렌즈용 금형을 가공하고자 하였다. 가공에는 3 축 구동의 초정밀 미세 복합가공기와 단결정 천연 다이아몬드공구가 사용되었고, 가공방식은 마이크로 세이핑 공정을 적용하였으며, 가공 금형 재료에는 Brass와 무전해 Nickel이 사용되었다. 실험을 통하여 금형가공시의 절삭력, 칩 형상, 가공표면 등의 분석이 수행되었으며 이를 기반으로 여러 가지 가공문제점을 해결하고, 최종적으로 양호한 렌티큘러렌즈용 금형을 가공하였다.

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Regulation of human gingival fibroblast gene expression on microgrooves: A DNA microarray study (마이크로그루브 상 인간치은섬유아세포의 유전자 발현 분석: DNA microarray 연구)

  • Lee, Kyungho;Leesungbok, Richard;Ahn, Su-Jin;Park, Su-Jung;Lee, Suk Won
    • The Journal of Korean Academy of Prosthodontics
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    • v.55 no.4
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    • pp.361-371
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    • 2017
  • Purpose: We aimed to investigate the gene expression of human gingival fibroblasts on microgroove surface using DNA microarray. Materials and methods: Microgrooves were applied on grade II titanium discs to have 0/$0{\mu}m$ (NE0, control group), 60/$10{\mu}m$ (E60/10, experimental group) of respective width/depth by photolithography. The entire surface of the microgrooved Ti substrata was further acid etched and used as the two experimental groups in this study. Human gingival fibroblasts were cultured in the experimental group and the control group, and total RNA was extracted. The oligonucleotide microarray was performed to confirm the changes of various gene expression levels between experimental group and control group. Changes of gene expression level were determined at the pathway level by mapping the expression results of DNA chips, using the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis. Results: Gene expression levels on E60/10 and NE0 were analyzed, there were 123 genes showing significant differences in expression more than 1.5 times on E60/10 microgrooved surface compared to NE0 surface, and 19 genes showing significant differences in expression more than 2 times. The KEGG pathway analysis confirmed the changes in gene expression levels under experimental conditions. Cell signaling, proliferation, and activity among the various gene expression results were identified. Conclusion: Microgrooved surfaces induce gene expression changes and related cell signaling. According to the results of this study, microgrooves can be used as the surface of various biomaterials which need to improve cell activity through gene expression changes and activation of cell signaling.

Effect of titanium surface microgrooves and thermal oxidation on in vitro osteoblast responses (마이크로그루브 및 열산화 복합 티타늄 표면의 골아세포분화 증진효과)

  • Seo, Jin-Ho;Lee, Richard sungbok;Ahn, Su-Jin;Park, Su-Jung;Lee, Myung-Hyun;Lee, Suk Won
    • The Journal of Korean Academy of Prosthodontics
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    • v.53 no.3
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    • pp.198-206
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    • 2015
  • Purpose: We aimed to investigate the effect of combined various microgrooves and thermal oxidation on the titanium (Ti) and to evaluate various in vitro responses of human periodontal ligament cells (PLCs). Materials and methods: Grade II titanium disks were fabricated. Microgrooves were applied on titanium discs to have $0/0{{\mu}m}$, $15/3.5{{\mu}m}$, $30/10{{\mu}m}$, and $60/10{{\mu}m}$ of respective width/depth by photolithography. Thermal oxidation was performed on the microgrooves of Ti substrata for 3 h at $700^{\circ}C$ in air. The experiments were divided into 3 groups: control group (ST), thermal oxidation group (ST/TO), and combined microgrooves and thermal oxidation group (Gr15-TO, Gr30-TO, Gr60-TO). Surface characterization was performed by field-emission scanning microscopy. Cell adhesion, osteoblastic differentiation, and mineralization were analyzed using the bromodeoxyurdine (BrdU), Alkaline phosphatase (ALP) activity, and extracellular calcium deposition assays, respectively. Statistical analysis was performed using the oneway analysis of variance and Pearson's bivariate correlation analysis (SPSS Version 17.0). Results: In general, the combined microgrooves and thermal oxidation group (Gr15-TO, Gr30-TO, Gr60-TO) showed significantly higher levels compared with the control (ST) or thermal oxidation (ST-TO) groups in the BrdU expression, ALP activity, and extracellular calcium deposition. Gr60-TO group induced highest levels of cell adhesion and osteoblastic differentiation. Conclusion: Within the limitation of this study, we conclude that the Ti surface treatment using combined microgrooves and thermal oxidation is highly effective in inducing the cell adhesion andosteoblastic differentiation. The propose surface is also expected to be effective in inducing rapid and strong osseointegration of Ti oral implants.

Characteristic of EP-MAP for Deburring of Microgroove using EP-MAP (전해-자기 복합 가공을 이용한 미세 그루브형상의 가공 특성에 관한 연구)

  • Kim, Sang Oh;Son, Chul Bae;Kwak, Jae Seob
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.37 no.3
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    • pp.313-318
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    • 2013
  • Magnetic abrasive polishing is an advanced deburring process for nonmagnetic materials and micropattern products that have non-machinability characteristics. Despite these advantages, there are some problems with using MAP for deburring. MAP has introduced geometric errors into microgrooves because of an over-cutting force caused by uncontrolled magnetic abrasives in the MAP tool. Thus, in this study, to solve this problem, an EP (electrolyte polishing)-MAP hybrid polishing process was developed for deburring microgrooves in an STS316 material. In addition, an evaluation of EP-MAP for the deburring of microgrooves was carried out by profiling the burrs. The results of the experiment showed geometric errors after the deburring process using MAP. However, in the case of EP-MAP, no geometric error was observed after the process because of the lower material removal rate in EP-MAP.

A Study on the Characteristics of Boiling Heat Trausfer of Thermosyphon Heat Exchangers with Various Micro Grooves (마이크로 그루브를 가진 열사이폰 열교환기의 비등열전달 특성에 관한 연구)

  • Cho Dong-Hyun;Lee Jong-Sun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.5 no.5
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    • pp.421-428
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    • 2004
  • This study concerns the characteristics of boiling heat transfer in two-phase closed thermosyphons with various micro grooves. A study was carried out with the performance of the heat transfer of the thermosyphon having 60 internal micro grooves in which boiling and condensation occur. A plain thermosyphon having the same inner and outer diameter as the grooved thermosyphon is also tested for comparison. Distilled water, methanol, ethanol have been used as the working fluid. The heat flux and the boiling heat transfer coefficient at the evaporator zone are estimated from the experimental results. The experimental results have been assessed and compared with existing correlations. Imura's and Kusuda's correlation for boiling showed in good agreement with experimental results within ${\pm}20{\%}$ in plain thermosyphon.

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A study on the effect of microgroove-fibronectin complex titanium plate on the expression of various cell behavior-related genes in human gingival fibroblasts (인간치은섬유아세포의 다양한 세포행동 관련 유전자발현에 마이크로그루브-파이브로넥틴 복합 티타늄표면이 미치는 영향에 대한 연구)

  • Hwang, Yu Jeong;Lee, Won Joong;Leesungbok, Richard;Lee, Suk Won
    • Journal of Dental Rehabilitation and Applied Science
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    • v.38 no.3
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    • pp.150-161
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    • 2022
  • Purpose: To determine the effects of the microgroove-fibronectin complex surface on the expression of various genes related to cellular activity in human gingival fibroblasts. Materials and Methods: Smooth titanium specimens (NE0), acid-treated titanium specimens (E0), microgroove and acid-treated titanium specimens (E60/10), fibronectin-fixed smooth titanium specimens (NE0FN), acid-treated and fibronectin-immobilized titanium specimens (E0FN), and microgroove and acid-treated titanium specimens immobilized with fibronectin (E60/10FN) were prepared. Real-time polymerase chain reaction experiments were conducted on 44 genes related to cell behavior of human gingival fibroblasts. Results: Adhesion and proliferation of human gingival fibroblast on microgroove-fibronectin complex titanium were activated through four types of signaling pathway. Integrin α5, Integrin β1, Integrin β3, Talin-2, which belong to the focal adhesion pathway, AKT1, AKT2, NF-κB, which belong to the PI3K-AKT signaling pathway, MEK2, ERK1, ERK2, which belong to the MAPK signaling pathway, and Cyclin D1, CDK4, CDK6 genes belonging to the cell cycle signaling pathway were upregulated on the microgroove-fibronectin complex titanium surface (E60/10FN). Conclusion: The microgroove-fibronectin complex titanium surface can up-regulate various genes involved in cell behavior.

Analysis of Thermal Performance in a Micro Flat Heat Pipe with Axially Trapezoidal Groove (그루브형 마이크로 히트파이프의 열전달특성에 대한 연구)

  • Suh, Jeong-Se
    • Proceedings of the KSME Conference
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    • 2001.06d
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    • pp.360-364
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    • 2001
  • Numerical Aanlysis is made on the thermal performance of micro heat pipe in a axial flat grooved channel. The flow of liquid and vapor is investigated in trapezoidal grooves and the effect of variable shear stress along the interface of the liquid and vapor considered. The results from this study are obtained in the axial variation of pressure difference between vapor and liquid, contact angle, velocity of liquid and vapor and so forth. In addition, maximum heat transport capacity of micro-heat pipe is provided by varying the operation temperature, and compared with that from Schneider and Devos's model in which the interfacial shear stress is neglected.

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A Study on the Performance of Boiling Beat Transfer of Inclined Thermosyphon Heat Exchangers with Internal Grooves (경사 열사이폰 열교환기의 비등열전달 성능에 관한 연구)

  • Cho, Dong-Hyun;Lee, Jong-Sun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.6 no.2
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    • pp.202-209
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    • 2005
  • This study concerns the performance of boiling heat transfer in inclined thermosyphons with internal grooves. A study was carried out with the performance of the heat transfer of the inclined thermosyphon having 60 internal grooves in which boiling and condensation occur. A plain thermosyphon having the same inner and outer diameter as the grooved thermosyphon is also tested for comparison. Distilled water, methanol and ethanol have been used as the working fluid. The inclination angle, three working fluids, heat flux and the boiling heat transfer coefficient at the evaporator zone are estimated from the experimental results. The experimental results have been assessed and compared with existing correlations. Imura's and Kusuda's correlation for boiling showed in good agreement with experimental results within ${\pm}20%$ in plain thermosyphon. The high heat transfer coefficient was found between $25^{\circ}$ and $30^{\circ}$ of inclination angle for water and between $20^{\circ}$ and $25^{\circ}$ for methanol and ethanol. The relatively high rates of heat transfer have been achieved in the thermosyphon with internal micro grooves.

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