• Proceedings of the Korean Society of Crop Science Conference
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• 2019.04a
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• pp.103-103
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• 2019

• Korean Journal of Plant Resources
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• v.34 no.1
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• pp.79-88
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• 2021

This research was conducted to investigate the genetic diversity and select useful accession with agronomic characteristics of garlic (Allium sativum L.). germplasms at Garlic Research Institute in Chungbuk Agricultural Research and Extension Service. Morphological diversity and relationships among 160 germplasms collected from 26 countries were assessed by methods of clustering and principal component analysis. Among 11 types of leaves and bulbs characteristics, emergence days of leaf showed the highest variation with coefficient of variation of 84.8%, and the bulb weight and the number of scales showed higher variability with 24.3%. Correlation analysis based on 11 quantitative traits showed that bulb weight and bulb length have very high positive correlation with bulb quantity. Plant height, leaf length, and number of leaves showed positive correlation with bulb weight as collections with better performance in growth produced large bulb with higher quality. The cluster analysis based on 5 principal components generated 6 clusters with an average distance of 1.6 among clusters. Domestic genetic resources were the largest with 36 species (22.5%) in group II.

• Applied Biological Chemistry
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• v.38 no.1
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• pp.49-54
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• 1995

To understand the molecular structure and pathogenesis mechanism of Korean garlic viruses, we have isolated cDNA clones for garlic viruses. The partial nucleotide sequences of 24 cDNA clones were determined and those of five clones containing poly(A) tail were compared with sequences of other plant viruses. One of these clones, V9, has a primary structure similar to the carlavirus group, suggesting that the clone V9 derived from a part of garlic latent virus (GLV). Northern blot analysis with the clone V9 as a probe demonstrated that GLV genome is 8.5 knt long and has a poly(A) tail. The clone V9 encodes coat protein (CP) of 33 kDa and nucleic acid binding protein of 10 kDa in different reading frame. The hexanucleotide motif, 5'-ACCUAA, which is conserved in the 3' noncoding region arid was proposed to be a cis-acting element involved in the production of negative strand genomic RNA was noticed. Complementary sequence to the hexanucleotide motif, 5'-TTAGGT, is also found in the positive strand of V9 RNA. The putative CP gene was cloned into the pRSET-A expression vector and expressed in E. coli BL21. The expressed recombinant V9CP protein was purified by $Ni^{2+}$ NTA affinity chromatography. The anti-V9CP antibody recognizes 34 kDa polypeptide which could be CP of GLV in infected garlic leaf extract. Immunoblot and Northern blot analysis of various cultivars shows wide occurrence of GLV in Korean garlic plants.

• Research in Plant Disease
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• v.30 no.2
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• pp.124-130
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• 2024

Leaf blight caused by Stemphylium vesicarium is one of the most important fungal diseases of garlic (Allium sativum L.) worldwide, which results in a reduction of quality and yield. The breeding of resistant cultivars is an efficient approach to decrease the use of chemical fungicides and minimize crop losses. In this study, to find the resistant garlic resources against S. vesicarium, we evaluated the resistance degree of 20 garlic germplasms. To do this, garlic seedlings at four-leaf stage were rubbed with nonabsorbent cotton and then inoculated with spore suspension (3.0×10⁵ spores/ml of potato dextrose broth) of S. vesicarium by spray method. Three to seven days after inoculation, the infected leaf area (%) of garlic seedling was measured. 'Daeseo' and 'Namdo' were included as susceptible and resistant control cultivars, respectively. After 3 to 7 days of incubation, the infected leaf area (%) of garlic seedling was measured. Our results showed that IT245512, IT245528, and IT244068 lines exhibited the highest resistance against S. vesicarium, whereas IT257134 and IT253043 lines were more susceptible than the susceptible cultivar 'Daeseo'. Based on the results, the resistant genetic resources selected in this study can be used a basic material for resistant garlic breeding system against leaf blight.

• Korean Journal of Food Science and Technology
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• v.53 no.6
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• pp.701-705
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• 2021

Although the breed protection rights of Jangsaemi garlic were registered in 2012, the cultivation area was not large and no related papers were found, so this study was conducted. Potassium was the highest inorganic content of Jangsaemi garlic, followed by nitrogen, sulfur, and phosphorus. Allin and allicin were also present at 9.73±0.97 and 1.65±0.15 mg/g, respectively. The solid content was 7.90±0.00°Bx, and the reducing sugar was 33.42±1.79 mg GE/g. The total phenol and flavonoid contents were 80.35±2.67 mg GAE/100 g and 33.80±2.17 mg QE/100 g, respectively. The antibacterial activity of Jangsaemi garlic was higher in gram-positive bacteria than in gram-negative bacteria, especially Staphylococcus epidermidis and Micrococcus luteus. The results of these studies are expected to provide useful information for farmers growing Jangsaemi garlic in the future.

• Food Science and Preservation
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• v.21 no.3
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• pp.321-327
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• 2014

This paper introduces the physicochemical characteristics of garlic shoots investigated in the 8 areas of Namhae, Gohyeon-myeon (GHM), Nam-myeon (NM), Samdong-myeon (SDM), Namhae-eup (NHE), Seo-myeon (SM), Seolcheon-myeon (SCM), Idong-myeon (IDM) and Changsoen-myeon (CSM). The moisture was $80.15{\pm}0.37{\sim}83.87{\pm}0.15g/100g$. The crude protein and lipid were $1.59{\pm}0.02{\sim}2.25{\pm}0.09g/100g$ and $0.35{\pm}0.01{\sim}0.48{\pm}0.03g/100g$, respectively. Ash was $0.65{\pm}0.03{\sim}0.71{\pm}0.03g/100g$. In addition, the total levels of phenol and flavonoid were searched as $33.02{\pm}1.78{\sim}58.41{\pm}1.08mg/100g$ and $11.60{\pm}3.36{\sim}23.95{\pm}2.04mg/100g$, respectively. The garlic shoots in IDM, where the highest total chlorophyll level detected as $170.58{\pm}1.04{\mu}g/g$ and in CSM, where the lowest total chlorophyll level investigated as $116.65{\pm}0.00{\mu}g/g$, were unveiled. The mineral elements (S, Ca, Fe, K, Mg, Na, Mn and Zn) were analyzed by ICP-OES, and K was the most highly contained mineral with $7257.0{\pm}87.9{\sim}10070.0{\pm}9.7mg/kg$. Significant feature of garlic shoots in IDM and NHE was that the total amounts of mineral were relatively higher than other regions as 21,143.5 mg/kg and 20,581.2 mg/kg, respectively. The cultivating regions were revealed as the one of important matters with the level of allicin ($78.03{\pm}6.15{\sim}203.43{\pm}18.47mg/kg$). In particular, the level of allicin of garlic raised in SDM ($203.43{\pm}18.47mg/kg$) was approximately 2.2~2.6 times higher than those in CSM ($92.86{\pm}8.70mg/kg$) and SCM ($78.03{\pm}6.15mg/kg$). The sulfur compounds content of diallyl trisulde was remarkably higher, especially the garlic shoots in IDM with $830.62{\pm}14.69{\sim}1281.10{\pm}27.71mg/kg$.

• Korean Journal of Food Science and Technology
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• v.29 no.5
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• pp.882-887
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• 1997

Quantitative determination of sulforaphane, S-methylsulfinylbutyl isothiocyanate in 20 cruciferous vegetables of Korean origin was performed. Homogenate of vegetable was extracted with dichloromethane, and the extract, after drying, was subjected to GC/MS analysis, which was based on single ion monitoring (SIM) at m/z 72, 160, 55, 114 and 177. The content of sulforaphane was found to be the highest in the extract of broccoli $(80.2{\sim}617.7\;ppm)$ followed by turnip $(15.4{\sim}23.1\;ppm)$, red cabbage $(9.9{\sim}32.1\;ppm),\;radish\;(5.5{\sim}8.8\;ppm)$ and kale (8 ppm). Among various cultivars of broccoli, broccoli '1243' and broccoli 'Pilgrim' showed higher content of sulforaphane than others. Especially, in the stem of broccoli '1243' and the floret of broccoli 'Pilgrim' the amount of sulforaphane was the highest (>700 ppm). Thus, the content of sulforaphane differed according to the cultivars and the portion of the vegetables.

• Research in Plant Disease
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• v.11 no.1
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• pp.28-34
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• 2005

This study was conducted to investigate the control possibility of garlic white rot causing severe yield losses of Allium species and cultivars using cultural practices such as optimal sowing date and burial depth, and lime application. Inoculum density in infested field soil was investigated at different soil depth, and that on the diseased plant debris was done. Inoculum density and recovery ratio of white rot pathogen of garlic was highly different between two species of Sclerotium cepivorum forming comparatively small sclerotia and Sclerotium sp. forming comparatively large ones. It was confirmed that S. cepivorum formed more sclerotia on bulbs of garlic than S. sp., and sclerotial recovery of S. cepivorum was higher than that of S. sp. Inoculum density of white rot pathogen in the infested field at garlic seeding period ranged from one to thirteen sclerotia per 30 g soil. Inoculum density of white rot pathogen decreased remarkably with increasing soil depth and above 95% of sclerotia were distributed within 5 cm of soil depth. Disease severity of white rot was higher on slightly planted garlics than deeply-planted ones. Garlic seed bulbs infected by white rot pathogens were confirmed to be one of main inoculum sources of white rot in the field and the disease incidences caused by garlic seed transmission showed big differences among garlic varieties. When nine garlic varieties harvested from infested plots were sown in the field, highly susceptible varieties, ‘Wando’, ‘Daeseo’, ‘Namdo’ and ‘Kodang’ showed high disease incidences, whereas other five varieties were not infected at all. It was confirmed that white rot occurred higher on early-sown garlics, before middle October, than on late-sown ones, after late October. Meanwhile, increasing application rate of lime ranged from 100 to 300 g reduced disease severity of white rot.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.5
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• pp.480-488
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• 2018

The development of information and communication technology has been carried out actively in the field of agriculture to generate valuable information from large amounts of data and apply big data technology to utilize it. Crops and their varieties are determined by the influence of the natural environment such as temperature, precipitation, and sunshine hours. This paper derives the climatic factors affecting the production of crops using the garlic growth process and daily meteorological variables. A prediction model was also developed for the production of garlic per unit area. A big data analysis technique considering the growth stage of garlic was used. In the exploratory data analysis process, various agricultural production data, such as the production volume, wholesale market load, and growth data were provided from the National Statistical Office, the Rural Development Administration, and Korea Rural Economic Institute. Various meteorological data, such as AWS, ASOS, and special status data, were collected and utilized from the Korea Meteorological Agency. The correlation analysis process was designed by comparing the prediction power of the models and fitness of models derived from the variable selection, candidate model derivation, model diagnosis, and scenario prediction. Numerous weather factor variables were selected as descriptive variables by factor analysis to reduce the dimensions. Using this method, it was possible to effectively control the multicollinearity and low degree of freedom that can occur in regression analysis and improve the fitness and predictive power of regression analysis.

• Bulletin of the Korea Photovoltaic Society
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• v.6 no.2
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• pp.15-24
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• 2020

영농형 태양광 발전은 농업과 태양광 발전 시스템의 동시 상생이 가능한 기술로 현재와 같은 농업 환경 및 생태계 개선 그리고 에너지 전환의 시대에 대처해야 하는 훌륭한 대안이다. 한국형 영농형 태양광 발전 시스템 개발 및 보급을 위한 국내 첫 연구개발 사업인 농림축산식품부(농림식품기술기획평가원 첨단생산기술개발사업)의 "한국형 태양광 이모작(농업&태양광 발전 병행) 스마트 영농 시스템 개발(과제번호: 1545015977)"과제를 통해 총 60kW급 규모의 5개소 실증단지(10kW급 3개소 신규 개발 및 구축)를 활용한 최적 시스템 개발, 6품종(벼 2품종, 감자, 배추, 마늘, 양파 및 배) 표준재배기술 도출 등을 수행하였다. 기존 태양광 발전용 모듈(72cell) 대비 half-size(36cell) 모듈 개발, 작물 별 최적 구조물(차광률 30% 미만 적용) 도출, 대상 작물 감수율 20% 미만 달성 및 표준재배기술개발을 수행하였다. 추후 장기간 동안 영농형 태양광 구조물의 경제성 확보, 시공성 개선 그리고 다양한 작물 대상 표준재배기술 최적화 등의 실증연구가 필요하다. 마지막으로 영농형 태양광 발전 시스템의 확산 및 보급을 위해 국가적 지원과 농민들의 적극적인 참여 없이는 불가능 하다. 그리고 본 개발 기술은 농업인을 위한 기술로 범국민적인 수용성 개선 또한 수반되어야 한다. 특히, 정부는 영농형 태양광 발전 시스템이 대한민국의 식량안보와 에너지안보를 확보를 위해 농업진흥지역에 대한 최대 20년까지 타용도 일시전용이 가능하도록 농지법 개정으로 화답 할 때이다.