• Horticultural Science & Technology
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• v.31 no.3
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• pp.380-387
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• 2013

This study was performed to examine the increasing biological activities of both nerve growth factor induction and anti-aging activity of gastrodia (Gastrodia elata) with fermentation process. Antioxidant activities, taken in the fermenting, were investigated to verify utility value of gastrodia for functional food and cosmetics. Fermented gastrodia extracts showed higher antioxidant activities than dried gastrodia extracts. During the routine check of all the practical use potential as functional food, inhibition effect of angiotensin converting enzyme (ACE) and xanthine oxidase (XOase) was tested among water extracted dried gastrodia (WEDG), water extracted fermented gastrodia (WEFG), 70% ethanol extracted dried gastrodia (EEDG) and 70% ethanol extracted fermented gastrodia (EEFG). DPPH was shown as WEDG = $64.14{\pm}0.89%$, WEFG = $66.21{\pm}1.03%$, EEDG = $82.25{\pm}0.52%$, and EEFG = $82.36{\pm}2.37%$. ABTS was shown as WEDG = $54.15{\pm}1.37%$, WEFG = $60.24{\pm}2.25%$, EEDG = $59.18{\pm}1.86%$, and EEFG = $77.17{\pm}4.23%$. Therefore, ACE activity was dramatically inhibited by EERG while there was no difference of XOase inhibition between EEFG and EERG. Nerve growth factor (NGF) activity was measured and indicated about 40% increased neurite growth effect. To conclude, biologically active compounds of gastrodia were increased by fermentation process. It seems to be that ferment gastrodia enhance the use ranges from functional food to fuctional cosmetics, and to all processing industry.

• Journal of Animal Science and Technology
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• v.54 no.5
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• pp.375-379
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• 2012

This study was carried out to define the "Wolla" coat color using 376 Jeju registered horses (white patched 142, solid coat color 234). Three major factors related to the white patches i.e ECA3-inversion for Tobiano, EDNRB 2 bp nucleotide substitution for frame Overo, and the KIT intron 16 single nucleotide polymorphism (SNP) for Sabino types of coat color were analyzed. It was found that out of 142 Jeju horses with white patches that have the genotype for ECA3-inversion (To) 140 horses were +/To heterozygous and 2 horses were To/To homozygous all Jeju horses with white patches had ECA3-inversion allele. However, there was no frame Overo or Sabino allele type in EDNRB and KIT intron 16 SNP in Jeju horses with white patches. As for 234 Jeju horses with a solid coat color, there was no ECA3-inversion allele related to the white patches. Thus, it could be considered that Wolla coat color with white patches in Jeju horses might have come from the Tobiano line in the genetic classification by coat color.

• Journal of the Korean Ceramic Society
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• v.41 no.5
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• pp.410-416
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• 2004

In this study, we fabricated magnetite coated on a cordierite honeycomb which has complex shape by ultrasound-enhanced ferrite plating. The effects of the plating condition on the formation of the magnetite and its microstructure were investigated. The magnetite coated on the honeycomb became an oxygen-deficient ferrite by H$_2$ gas reduction, then the effects of the molar concentrations of ammonium acetate for $CO_2$ gas decomposition have been studied. As the molar concentration of a pH buffer($CH_3$COONH$_4$, 0.1946∼0.3892 M) solution increased, the average particle size increased about 200∼250 nm. The magnetite coated on the honeycomb was reduced by H$_2$ gas for 2 h at 30$0^{\circ}C$. The inner pressure change in the cell began to occur at 315∼34$0^{\circ}C$. The H$_2$-Reduced magnetite coated on the honeycomb at 35$0^{\circ}C$ contained an oxygen deficient magnetite and $\alpha$-Fe phase. The thermogravimetric analysis with H$_2$ reduction and $CO_2$ decomposition were carried out with the magnetite coated on the honeycomb. A weight loss in process of H$_2$ reduction occurred between 32$0^{\circ}C$ and 34$0^{\circ}C$, while a weight gain was observed during the $CO_2$ decomposition.

• Journal of Life Science
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• v.28 no.1
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• pp.130-139
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• 2018

In ancient times, horse racing was done in ancient European countries in the form of wagon races or mountain races, and wagon racing was adopted as a regular event at the Greek Olympic Games. Thoroughbred horse has been bred since 17th century by intensive selective breeding for its speed, stamina, and racing ability. Then, in the 18th century, horse racing using the Thoroughbred species began to gain popularity among nobles. Since then, horse racing has developed into various forms in various countries and have developed into flat racing, steeplechasing, and harness racing. Thoroughbred racehorse has excellent racing abilities because of powerful selection breeding strategy for 300 years. It is necessary to maintain and maximize horses' ability to race, because horse industries produce enormous economic benefits through breeding, training, and horse racing. Next-generation sequencing (NGS) methods which process large amounts of genomic data have been developed recently. Based on the remarkable development of these genomic analytical techniques, it is now possible to easily carry out animal breeding strategies with superior traits. In order to select breeding racehorse with superior racing traits, the latest genomic analysis techniques have to be introduced. In this paper, we will review the current efforts to improve race performance for racehorses and to examine the research trends of genomic analysis. Finally, we suggest to utilize genomic analysis in Thoroughbred racehorse and Jeju horse, and propose a strategy for selective breeding for Jeju horse, which contributes job creation of Korea.

• Korean Journal of Microbiology
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• v.50 no.1
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• pp.46-52
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• 2014

This study was carried out to investigate the characteristics of fermented chinese yam and rice (Dioscorea japonica, Dioscorea batatas, Dioscorea opposita, Rice, FCYR) using Monascus sp. MK805. The extracts from FCYR were measured to examine pigments, antioxidant activities were investigated through DPPH radical scavenging activity, total polyphenol and flavonoid contents, reducing power. Also it was investigated monacolin K productivity by FCYR. Pigments productivity (yellow, orange and red) were 26.2, 13.9, 17.3 at Dioscorea japonica, 41.9, 22.6, 53.2 at Dioscorea batatas, 12.5, 7.5, 9.7 at Dioscorea opposita and 10.1, 7.7, 10.2 at rice, respectively. DPPH radical scavenging activity of FCYR was about 69.7, 79.6, 57.8, and 42.3%, total polyphenol contents of FCYR was about 480.6, 658.7, 379.3, and 212.9 mg/kg, total flavonoid contents of FCYR was about 342.5, 448.4, 235.2, and 168.7 mg/kg, reducing power of FCYR was about 1.57, 2.14, 1.14, and 0.35 (OD at 700 nm), respectively. And then monacolin K productivity of FCYR was about 453.8, 509.5, 332.2, and 263.2 mg/kg, respectively.

• Korean journal of food and cookery science
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• v.14 no.4
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• pp.333-338
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• 1998

On the mutagenicity induced by 2-aminofluorene (2-AF) with S9 mix and N-Methyl-N＇-Nitro-N-Nitrosoguanidine (MNNG) without S9 mix, the antimutagenic effects of dietary fiber (total dietary fiber, u-cellulose and pectin) from Yam were examined by the Ames assay using Salmonella typhimurium TA98 and TA100. Total dietary fiber, $\alpha$-cellulose ind pectin from natural and cultural Yam didn＇t have mutagenicity. Most of sample dietary fiber showed the antimutagenicity. Total dietary fiber from cultural Yam was more effective than that from natural Yam on mutagenicity induced by 2-AF and MNNG. $\alpha$-cellulose from cultural Yam was more effective than that from natural Yam on mutagenicity caused by 2-AF and MNNG. Pectin from natural and cultural Yam had antimutagenic effect on mutagenicity induced by MNNG. In this study, antimutagenicity on MNNG was more effective than that on 2-AF. Antimutagenic effect of Samples had influence on incubation time. $\alpha$-cellulose and pectin from natural and cultural Yam showed stronger antimutagenic effect than standard $\alpha$-cellulose and standard pectin, respectively, on mutagenicity induced by 2-AF and MNNG.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.4
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• pp.365-374
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• 2009

This study was carried out in thoroughbred colts grazed in pasture at summer season of Jeju island, Korea, from June 23 to 29 in 2007. We investigated the behavioral pattern of the thoroughbred colts (n=23) at the age of 3-year-old (n=11) and 1-year-old (n=12). The group behavioral pattern of thoroughbred colts was different according to the age. It was shown that eating 56%, standing 23%, lying 10%, walking 5%, running 2.5% and drinking 3% in the 1-year-old thoroughbred colts group. Especially, it was significantly individual difference (P<0.05) in the average behavioral expression ratio of 1-year-old thoroughbred colts (n=3), which was eating 53%, standing 29%, lying 11%, walking 5%, drinking 1% and running 1%. On the other hand, it was investigated that eating 54%, standing 27%, walking 13%, running and drinking 1.5%, lying 1% in the 3-year-old thoroughbred colts group. Also, the individual behavioral expression ratio of the 3-year-old thoroughbred colts was eating 53%, standing 32%, walking 10%, running and drinking 1%, which was a similar pattern to the result of 1-year-old colts. It is considered that this is the first study regarding a pasturage action of a racehorse produced in Korea. These data cannot be enough, but this data will be based on the production of racehorses and fostered in the management.

• Journal of the Korean Magnetics Society
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• v.16 no.1
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• pp.102-106
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• 2006

Magnetic nanoparticles can be used for a variety of biomedical applications. They can be used in the targeted delivery of therapeutic agents in vivo, in the hyperthermic treatment of cancers. in magnetic resonance (MR) imaging as contrast agents and in the biomagnetic separations of biomolecules. We have synthesized magnetite $(Fe_3O_4)$ nanoparticles using chemical coprecipitation technique with sodium oleate as surfactant. Nanoparticle size can be varied from 2 to 8nm by controlling the sodium oleate concentration. Magnetite phase nanoparticles could be observed from X-ray diffraction. Magnetic colloid suspensions containing particles with sodium oleate and chitosan have been prepared. Nanoparticles, both oleate-coated and chitosan-coated, have been characterized by several techniques. Atomic farce microscope (AFM) was used to image the coated nanoparticles. Magnetic hysteresis measurement were performed using a superconducting quantum interference device (SQUID) magnetometer at room temperature to investigate the magnetic properties of the magnetite nanoparticles. The SQUID measurements revealed superparamagnetism of nanoparticles.

• Journal of Life Science
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• v.17 no.10
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• pp.1441-1446
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• 2007

This study was conducted to establish the individual identification system and to estimate the genetic characteristic of Jeju native horse (JNH) using 13 microsatellite markers located on different horse autosomes. The markers were genotyped on 191 animals from five horse breeds including Jeju native horse (JNH). In total, 138 alleles were detected from the genotypes of 13 microsatellite markers. The average heterozygosities ranged from 0.317 to 0.902 and the polymorphic information content (PIC) ranged from 0.498 to 0.799 in JNH. We found that there were significant differences in allele frequencies in JNH when compared with other horse breeds. In ATH4 marker, there were specific allele frequence pattern that some of allele only found in JNH, Mongolian horse (MONG) and Jeju racing horse (JRH). The calculated cumulative power of discrimination (CPD) was 99.9% when nine microsatellite loci were used for analysis in the individual identification system. Also, the matching probability that two unrelated animals would show the same genotypes, was estimated to be $0.60{\times}10^{-10}$. Therefore, in the nine markers used in this study can be used for individual identification in the Jeju native horse population.

• Korean Journal of Medicinal Crop Science
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• v.8 no.4
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• pp.362-372
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• 2000

This study was conducted to obtain the basic information on the production of high yield and qualitied yam in Andong, major yam-producing district, in Kyongbuk province, using three major Yam cultivars, such as Danma, Jangma and Alata (round type). Alata showed the longest tuber width while Jangma showed the longest tube length. Danma showed the highest tube yield at 2,123 kg/10a. Tubes of Alata had higher dry ratio than those of other cultivars, suggesting that Alata could be used as good processing materials. Characteristics of top parts, such as vine length, leaf number, and lateral vine number showed typical sigmoid curves. Tuber characteristics such as length, width and fresh weight of tuber were linearly increased as the growth period was progressed. Relative growth rate (RGR) for new tuber and stem reached the highest value at 86 DAT (days after transplanting). There were similar tendencies among the cultivars in net assimilation rate (NAR). The highest crop growth rate (CGR) was appeared at 156 DAT regardless of cultivars. In the changes of leaf area ratio (LAR), there were significant differences among three cultivars. LAR were decreased at 86, 106 and 136 DAT for Alata, Danma and Jangma, respectively. However, specific leaf weight (SLW) was greatly increased at 176 DAT, and leaf area index (LAI) was decreased at 156 DAT in all cultivars.