• KSCE Journal of Civil and Environmental Engineering Research
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• v.29 no.1B
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• pp.97-103
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• 2009

The decompostion characteristics of NAPL TCE in cement/slag/Fe(II) system were studied with various TCE concentration and amounts of binders (cement/slag) For analyses of the TCE degradation by cement/slag/Fe(II), TCE solution injected using gas-tight syringe after TCE solution dissolved a methanol. Initial concentrations of TCE are 0.42 mM, NAPL condition 11.7 mM and saturated condition 16.8 mM respectively. The result showed that the cases of 8.4 mM and 4.2 mM are decreased 88% of total TCE concentration within 18 days. NAPL condition 11.7 mM was decreased 84% within 50 days and saturated condition 16.8 mM was decreased 60% of total TCE concentration within 60 days respectively. This showed that degradations of TCE in various concentrations were in one kind reaction as pseudo-first-order. TCE was dissolved as aqueous solution before degraded. The reaction rate was increased $0.12day^{-1}$, $0.24day^{-1}$, $0.31day^{-1}$ when the mass of media 0.1, 0.2, 0.3 S/L rate was increased. TCE reaction speed is affected by cement/slag surface ares in this system. When HDTMA, experimental facter, was added, TCE decomposition rate was high despite the high concentration of NAPL. and The decompostion characteristics of NAPL TCE in cement/slag/Fe(II) system were studied by using modeling.

• Journal of Korea Foresty Energy
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• v.20 no.1
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• pp.28-34
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• 2001

To analyze chemical compositions of softwood vinegar prepared with continuous carbonized kiln, the chemical compositions were analyzed by GC-MS spectrometry. The results were summarized as follow : 1. The amounts of methylalcohol and acetic acid and of vinegar were 0.12% and 0.8% respectively, and acidity was 0.85 2. Perfume components of vinegar were frufual, 5-meayl-2-furancarboxyaldehyde, 2,3-pentanedione, 2-butanol, 2,3-dihydrofuran, 1-(2-furanyl)-etanone, benzaldehyde, 2-furan carboxyaldehyde and acetic acid. 3 Vinegar prepared from softwood, so that murk amount of guaiacyl compound and phenol derivetives are produced from lignin and extractives was analγzed. 4 The yield of 4-methyl-di-tert-butylphenol was the highest in the nutural and carbonyl and acetic acid in the acid fractions, 3-ethylpentane in the basic fraction, and guaiacol in the phenolic fraction.

• Korean Journal of Environmental Biology
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• v.31 no.2
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• pp.158-164
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• 2013

A microcystin-degrading bacterium was isolated from Daechung reservoir, Korea. The isolated bacterium was identified as Microbacterium sp. by 16S rRNA gene sequence analysis, and designated as Microbacterium sp. MA21. This strain degraded cyanobacterial hepatotoxin, microcystin-LR, over 80% when incubated at $30^{\circ}C$ for 12 hr in R2A medium. Two unknown metabolites of microcystin were also identified during the degradation process. Although only Sphinogomonas and Actinobacteria have been known to degrade microcystin previously, this is the first report that Microbacterium sp. MA21 could degrade microcystin.

• Journal of Life Science
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• v.10 no.3
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• pp.300-306
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• 2000

Several bacterial strains utilizing crude oil as their sole carbon and energy source were isolated from polluted marine by crude oil. One of the strains, named KCL-2 showed strong degradation activity for crude oil. This strain was identified as a Klebsiella sp. based on the morphological, biochemical, and physiological characteristics. The optimum cultural conditions were as follows; $27^{\circ}C$~$37^{\circ}C$ for temperature and 7.0 for initial pH. Additionally, the optimal concentration of sodium chloride was 3.0%, confirming indicating that this strain was derived from sea water.The strain KCL-2 could use several kinds of n-alkane hydrocarbones from octadecane to octacosane as a sole carbon source. The emulsifying activity by KCL-2 was the highest after 3 days of cultivation under the condition of 3.0% sodium chloride, pH 7.0 and 32$^{\circ}C$. This strain had several criptic plasmids.

• Korean Journal of Food Science and Technology
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• v.20 no.5
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• pp.650-658
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• 1988

The mobilization of proteins in the cotyledons of germinating soybean seeds (Glycine mar [L.] Merr.) and seedlings was studied by using light microscopy and transmission electron microscopy. The cotyledon tissues of soybean. were packed with protein bodies(diameter $0.1-15{\mu}m$) where storage protein of soybean is deposited. Degradation of protein bodies started in the epidermis and vascular tissues. After swelling of the protein bodies, autolysis of storage proteins began while the external membrane remained unbroken. Hydrolysis of proteins could be internal or peripheral and fusion might begin before complete protein degradation. Possible instances of vacuolar fusion were encountered in some cells. In all cases, the result of degradation was the same; the central vacuole of the cell. At the late stages of seedling growth, breakdown of tonoplast was observed in some cells.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.733-736
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• 2000

Lignin is usable as fuels and heavy oil additives if depolymerized to monomer unit, because the chemical structures are similar to high octane materials found in gasoline. In this study, the solvent-phase thermal cracking(solvolysis) of lignin was performed at the various temperature and time in a laboratory tubular reactor. Conversion yield was measured for the properties of thermal cracking and liquefaction reaction of lignin. Highest conversion yield when acetone was used as thermal cracking solvent was 55.5% at $350^{\circ}C$, 50minutes and highest tar generation were $260{\sim}350mg/g\;{\cdot}\;lignin$ at $250^{\circ}C$, and highest conversion yield after tar removal was 76.88% at $300^{\circ}C$, 30minutes. Conversion yield, product compositions and amounts were determined by tar degradation yield.

• Korean Journal of Microbiology
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• v.30 no.1
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• pp.47-52
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• 1992

This study describes isolation and identification of a soil bacterium which is degradable of phosphinothricin and improvement of the isolated strain by using mutagenesis and spheroplast fusion. The experiment was performed to search for a possibility of development of a new strain which is both PPT-degradable and glyphosate-resistant by using interspecies cell fusion between the PPT-degrading bacterium. Pseudornonu.\ puucimohlis and a glyphosate -resistant strain, Pseudornonu.~ cc,pucicl. Auxotrophic mutants were obtained by the treatement of P. puucimohili.\ with ethylmethanosulfate, and used to cell fusion. Lysozyme and EDTA were used to spheroplast formation and regeneration rates :)f the spheroplast were 6.5'%1 in P. pauc.irnohili.\ and 8.8% in P.ci,j~u[,i(lr, espctively. Polyethylenglycol 5.000 was used to cell fusion as fusogen. The fusant\ulcorner F1. F2. F\ulcorner and F4 werc- obtained by the intra- and interspecies cell fusion. The fusant Fl of intraspecies cell fusion was higher to the wild type by 1 I'%l in PPT degrading ability, and the fusant F3 of inierspesis cell fusion developed plyphosatc-resistant and PPI-dcgrading ability which were propertics of two parental strains.

• Journal of Life Science
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• v.11 no.6
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• pp.530-536
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• 2001

Several bacterial strains utilizing crude oil as their sole carbon and energy source were isolated from marine environment polluted by crude oil. Among them, the selected strain 4A3 showed strong degradation activity for crude oil. This strain was identified as a Provindencia rettgeri 4A3 based on the morphological, biochemical, and physiological characteristics. The optimum cultural conditions was as follows; 26$^{\circ}C$ for temperature and 7.0 for initial pH. Additionally, the optimal concentration of sodium chloride was 2.0%, indicating that this strain was derived from sea water. The emulsifying activity of 4A3 was the highest after 3 days of cultivation under the condition of 2.0% sodium chloride, pH 7.0 and 26$^{\circ}C$. This strain had one cryptic plasmid in 7.0kb size.

• Journal of Environmental Health Sciences
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• v.26 no.2
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• pp.14-21
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• 2000

DEAE-Toyopearl 650S, Superdex pg-75, Poros HQ, Superdex H200의 각종 칼러크로마토그래피를 이용하여 C.bifermentans DPH-1균주로부터 테트라클로로에틸렌(PCE) 분해 효소를 정제했다. 이 PCE 분해효소 (PCE dehalogenase)는 PCE를 환원적 탈염소화 반응에 의해 시스디클로로에딜렌 (cis-1,2-dichloroethylene)에 전환 가능하여, 이 때의 Vmax 및 Km 치는 각각 73 nmol/h.mg protein, 12$\mu$M이었다. 본 PCE dehalogenase의 겔여과 분자량 Maker Kit를 이용한 분석결과(70kDa)는 SDS-PAGE에 나타난 분자량(35kDa)의 약 2배인 것으로 확인되었다. 따라서 본 효소는 분자량 70kDa의 이량체(Homo dimer)인 것으로 추정되었다. 본 효소의 최적온도 및 pH는 각각 35$^{\circ}C$ 및 8.0 이었다. 또한 본 효소는 PCE외의 트리클로로에틸렌, 디클로로에틸렌 이성체, 1,2-디클로로에템, 1,2-디클로로프로판, 1,1,2-트리클로로에탄에 대하여도 활성을 타나내었다. N-말단 아미노산 분석결과에서도 본 효소는 현재 알려진 PCE dehalogenase와 그 배열이 전혀 다른 것으로 나타나 각종 유기염소 화합물의 분해능을 보유한 신종의 PCE 분해효소인 것이 확인되었다.

• Journal of Life Science
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• v.28 no.5
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• pp.569-576
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• 2018

This study was carried out to investigate the antioxidative, nitrite-scavenging, alcohol-metabolizing, and anti-inflammatory effects of black-cherry tomato juice (BCTJ) on LPS-induced RAW 264.7 cells. The total phenol content of the BCTJ was $156.83{\mu}g\;tannic-acid-equivalent/ml$. The antioxidative effects of BCTJ were measured using DPPH radical-scavenging activity and SOD-like assay. DPPH radical-scavenging activity of BCTJ was increased in a dose-dependent manner (p<0.05) and was 83.39% at 40%. SOD-like activity of BCTJ was 22.01% at 100%. The effects of BCTJ on alcohol-metabolism were determined by measuring generations of reduced nicotinamide adenine dinucleotides (NADH) by alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH). ADH and ALDH activities were 198.87% and 181.89% at 40%, respectively. Nitric scavenging activities of BCTJ were 85.06%, 58.25%, and 43.68% at pH values 1.2, 3.0, and 6.0, respectively, at 50%. Anti-inflammatory effects were examined in LPS-stimulated RAW 264.7 cells. Nitric-oxide production was reduced to 83.55% by the addition of BCTJ at 10%. These results suggest that black-cherry tomato juice has great potential as a resource for natural health products.