• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.46 no.4
• /
• pp.403-413
• /
• 2020

While searching for useful microorganisms, Saccharomycopsis fibuligera which can be used as cosmetic materials were divided from Jeju island's traditional fermented foods. In this study, blackcurrant extract which contains a large amount of anthocyanin glycosides was fermented with S. fibuligera. HPLC analysis was performed to analyze the components of blackcurrant extract (BE) and fermented blackcurrant fruit extract (FBE). As a result, bio-conversion of delphinidin and cyanidin were able to be identified. In order to verify the anti-oxidant effect of BE and FBE, we investigated radical scavenging ability with DPPH and ABTS. In addition, to confirm anti-inflammatory effect, we investigated inhibition effect of nitric oxide (NO) production on lipopolysaccharide (LPS) - stimulated RAW264.7 macrophages, and inhibition effect of the expression of inflammatory-related proteins (iNOS, COX-2) by western blot analysis. As a result, as FBE has anti-oxidant and anti-inflammatory effects, we suggest that it might be used as an active ingredient for cosmetics.

• Journal of the Korea Convergence Society
• /
• v.13 no.3
• /
• pp.281-289
• /
• 2022

Plasma ozone is utilized in a variety of applications in the field of sterilization due to its high sterilization performance. Dielectric materials used in DBD(dielectric barrier discharges) are mainly polymer, quartz and ceramics. These dielectric layers have the advantage of limiting the amount of supplied electron charge and allowing plasma to occur evenly on the surface of dielectric. Actually, the target or environment for sterilization is often a complex structure, so research and academic study are needed by utilizing the concept of space sterilization. In this study, the device is applied to generate DBD plasma at atmospheric pressure for disinfection due to the effectiveness in producing radicals and ozone. The generator of plasma ozone is a basic structure of dielectric barrier discharge by placing ceramic tube dielectrics and stainless steel electrical conductors at regular intervals. Various applications can be developed based on the proposed design method. Plasma ozone generation for space sterilization device is recognized as an excellent sterilization device. Through the design and verification of the device, we intend to establish an optimal design of the spatial sterilization device and provide the basis data for sterilization applications.

• Applied Chemistry for Engineering
• /
• v.33 no.4
• /
• pp.444-450
• /
• 2022

The effect of coated polyester (PET) textiles with metal oxide, chitosan, and copper ion on the antibacterial and antiviral activities was evaluated to investigate the applicability of multi-coated PET textiles as antiviral materials. Compared to coated PETs with a single agent, multi-coated PETs reduced the loading amount of coating materials as well as the contact time with bacteria for a bacterial cell number of < 10 CFU/mL, which was not detectable with the naked eyes. Metal oxides generate reactive oxygen species (ROS) such as free radicals by a catalytic reaction, and copper ions can promote contact killing by the generation of ROS. Chitosan not only enhanced antibacterial activities due to amine groups, but enabled it to be a template to load copper ions. We observed that multi-coated PET textiles have both antibacterial activities for E. coli and S. aureus and antiviral efficiency of more than 99.9% for influenza A (H1N1) and SARS-CoV-2. The multi-coated PET textiles could also be prepared via a roll-to-roll coating process, which showed high antiviral efficacy, demonstrating its potential use in air filtration and antiviral products such as masks and personal protective equipment.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.49 no.4
• /
• pp.299-306
• /
• 2023

In this study, exosomes were isolated by ultrafiltration from Lactobacillus rhamnosus J2K-821 and their various effects for skin were evaluated. Their size and concentration were identified 50 ~ 200 nm and 3.22 × 10⁸ particles/mL, respectively through nanoparticle tracking analysis. In order to verify the inflammatory relief effect of Lactobacillus rhamnosus-derived exosomes (LRDEs), their nitric oxide (NO) production inhibitory ability in RAW 264.7 macrophages induced an inflammatory reaction with lipopolysaccharide (LPS) was confirmed. It was revealed that they inhibited NO production in a concentration-dependent manner. To evaluate the antioxidant activity and skin barrier improvement effect of LRDEs, their 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and hyaluronidase inhibitory activity were confirmed. It was also revealed that their activities were increased by concentration-dependent manner. Through these results, It is believed that LRDEs can be used as a effective natural cosmetic ingredient for anti-inflammation, antioxidation and skin barrier improvement.

• Journal of Life Science
• /
• v.34 no.4
• /
• pp.236-244
• /
• 2024

Melanin is a natural pigment found in most plants and animals, and it is involved in determining the color of the skin and hair. Melanogenesis is a reactive occurrence in melanocytes aiming to protect the skin from external stimuli, such as ultraviolet rays. Tyrosine is involved in the biosynthesis of the substrate tyrosine into melanin. However, melanin overproduction can lead to skin diseases, such as melasma, blotching, hyperpigmentation, and skin cancer. Although many studies have been conducted on whitening substances, such as kojic acid and arbutin, some countries have banned or refrained from using them due to their side effects. Therefore, this study assessed the potential of horseradish (HR) as a new whitening agent in cosmetic products. For efficient extraction, subcritical water extraction was conducted. The results showed that the horseradish subcritical water 200℃ (SW 200) extract showed high DPPH radical scavenging ability, total phenolic contents (TPC), inhibiting tyrosinase activity and inhibiting melanin production of B16-F10 melanoma cell lines. To investigate its cytotoxicity to the B16-F10 melanoma cell lines, MTT reduction assay and morphological changes were observed. No cytotoxicity was found in horseradish methanol extract and SW 200. In conclusion, this research suggests the possibility of horseradish subcritical water may be useful as a natural whitening ingredient to be used in cosmetic products.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.50 no.1
• /
• pp.19-27
• /
• 2024

This study is to propose Lagerstroemia indica flower as an anti-acne cosmetic material, and confirmed the sebum control, anti-inflammatory, and antioxidant effects using Lagerstroemia indica flower extract (LIFE) . As a result of evaluating the sebum control effectiveness, it was confirmed that LIFE inhibited the production of sebum excessively induced with palmitic acid up to 65%. Subsequently, it was confirmed that LIFE has an antibacterial effect and the ability to inhibit lipase activity against Cutibacterium acnes (C. acnes), which mainly appears in acne lesions, and can also reduce the inflammatory response caused by virulence factors secreted by C. acnes. It was also confirmed that LIFE inhibited the secretion of nitrogen monoxide (NO) and prostaglandin E2 (PGE2), which are inflammatory mediators induced by LPS in macrophages, by 75% and 54%, respectively, and that it also had a high DPPH radical scavenging ability similar to that of ascorbic acid. These results suggest that LIFE, a natural extract, can be used as an anti-acne material to relieve and treat acne, a complex disease, by controlling sebum production and having antibacterial and lipase activity inhibiting against C. acnes, and anti-inflammatory, antioxidant properties.

• Journal of the Korean Applied Science and Technology
• /
• v.41 no.2
• /
• pp.159-171
• /
• 2024

This study confirmed the antioxidant activity and antimicrobial efficacy and formulation stability for the effectiveness experiment of Rumex crispus. L root extract. For antioxidant activity, DPPH radical scavenging, FRAP activity, ABTS+ radical scavenging, and SOD-like activity were performed. Antimicrobial activity was evaluated for Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans strains. In addition, skin containing Rumex crispus. L root extract is checked over time for pH, temperature, and daylight for 21 days. As a result of antioxidant evaluation, it was confirmed that the activity increased in a concentration-dependent manner at a concentration of 0.0625-1 mg/mL. The clear zones of each bacterium at 100mg/mL concentrations were 10.45±0.34, 9.77±0.59, 9.92±0.22, and 10.08±0.12, which were superior to the control group Methyl paraben, and the antibacterial power of S. aureus and E. coli was confirmed at 100mg/mL concentration for MIC. There was little change in absorbance when the pH of the skin was 4.0, 6.0, and 7.0 and At 4℃, 25℃, and 40℃, it was discolored as the temperature increased. It was also observed that discoloration occurred when exposed to daylight. This is presumed to be able to prevent discoloration when it is shielded and stored at low temperatures. When the results of this study are summarized, Rumex crispus. L root extract is considered to have high value in use as a cosmetic raw material that can expect antioxidant and antibacterial activities.

• The Korean Journal of Pharmacology
• /
• v.28 no.2
• /
• pp.201-212
• /
• 1992

Effect of exogenous taurine on HOCl, $NH_2Cl$ and other oxidants-induced degradation of hyaluronic acid was investigated. The scavenging action of taurine on HOCl, $NH_2Cl$ and other oxidants was examined. The antioxidant action of taurine was also compared with that of thiol compounds. Viscosity of hyaluronic acid was markedly decreased by HOCl and $NH_2Cl$ on a dose dependent fashion. The degradative effect of HOCl on hyaluronic acid was greater than that of $NH_2Cl$. Taurine effectively inhibited HOCl-and $NH_2Cl-induced$ degradation of hyaluronic acid in a dose dependent fashion. The degradative effect of HOCl was markedly inhibited by DMSO. $Fe^{2+}$ plus $H_2O_2-induced$ degradation of hyaluronic acid was inhibited by catalase and DMSO but not affected by taurine. The desradative action of xanthine and xanthine oxidase was effectively inhibited by SOD and catalase but not affected by taurine. HOCl was significantly decomposed by taurine, DMSO, GSH and MPG. Both absorbance of HOCl at 250 nm and absorbance of $NH_2Cl$ at 242 nm were significantly increased by the addition of taurine. Interaction of $NH_2Cl$ with GSH or MPG showed an initial peak absorbance, but these absorbances were gradually decreased with time. OH production in the presence of $Fe^{2+}$ and $H_2O_2$ was inhibited by catalase and DMSO but not affected by taurine. Taurine did not affect $^1O_2$ production by U.V. irradiation which is responsible for DABCO and DABA. GSH and MPG markedly inhibited the degradative action of HOCl. These results suggest that the protective action of taurine on oxidants-induced damages of tissue components, including degradation of hyaluronic acid may be attributable to both its scavenging action on HOCl and $NH_2Cl$ and the complex formation of taurine with HOCl or $NH_2Cl$ without scavenging action on oxygen free radicals. Sulfhydryl group of taurine appears to show partially a protective action on HOCl-and $NH_2Cl-induced$ degradation.

• The Korean Journal of Food And Nutrition
• /
• v.23 no.1
• /
• pp.30-38
• /
• 2010

This study was conducted to determine the effects of vitamin C on the activity of liver function enzymes and electromicrographic changes in white rats treated with aflatoxin $B_1(AFB_1)$ or X-ray and $AFB_1$. Six week-old male Sprague-Dawley rats were randomly divided into five groups: a control group, $AFB_1$ treated group, $AFB_1$ treated group with vitamin C, X-ray and $AFB_1$ co-treated group, X-ray and $AFB_1$ co-treated group with vitamin C. On the first day of the experiment, only one dose of X-rays was exposed to the entire liver at 1,500 cGy. Next, vitamin C was injected at 10 mg/kg body weight by intraperitoneal injection, followed 1 hr later by the administration of 0.4 mg/kg of $AFB_1$ by intraperitoneal injection. These treatments were then administered every three days over a period of 15 days. On the 16th day of treatments, the animals were sacrificed. Analysis of the activity of the liver function enzymes, GOT, ALK phatase and LDH, in the sera of rats revealed that they were somewhat increased by $AFB_1$ treatment, X-ray and $AFB_1$ co-treatment when compared to the control group. Furthermore, the activity of these enzymes decreased in response to administration of vitamin C. Especially, the levels of GOT were remarkably decreased in the $AFB_1$ treated group treated with vitamin C when compared to the group treated with $AFB_1$ alone(p<0.001). Electromicrographic analysis revealed cloudy swelling, necrosis, vesicular degeneration and fat accumulation of hepatocytes in response to treatment with $AFB_1$ or co-treatment with X-ray and $AFB_1$. However, the destruction of hepatic cells was considerably lower in the vitamin C-treated group. These results indicate that vitamin C had ameliorating effects on the hepatic cell damage.

• Food Science and Preservation
• /
• v.24 no.5
• /
• pp.623-630
• /
• 2017

Functional compounds including flavonoids, anthocyanins, polyphneols and antioxidants were extracted from blue honeysuckle (Lonicera caerulea L.) using highly efficient microwave-assisted extraction. And extraction process was modeled and optimized according to response surface methodology (RSM). The independent variables ($X_n$) were ethanol concentration ($X_1$: 0, 25, 50, 75, 100%), irradiation time ($X_2$: 1, 3, 5, 7, 9 min), and microwave power ($X_3$: 60, 120, 180, 240, 300 W). Dependent variables ($Y_n$) were total flavonoid contents ($Y_1$), total anthocyanin contents ($Y_2$), total polyphenol contents ($Y_3$) and antioxidant activity ($Y_4$). Four-dimensional response surface plots were generated based on the fitted second-order polynomial models to get optimal conditions. Estimated optimal conditions for 4 responses were ethanol concentration of 54-72%, irradiation time of 7.1-7.6 min, and microwave power of 243-251 W. Ridge analysis predicted the maximal responses of total flavonoid content, total anthocyanin content, total polyphenol content and antioxidant activity were 38.00 mg RE/g, 6.80 mg CGE/g, 14.90 mg GAE/g, 89.10%, respectively. Verification experiment was carried out at predicted optimal conditions and experimental values for total flavonoid content, total anthocyanin content, total polyphenol content and antioxidant activity were 38.10 mg RE/g, 6.72 mg CGE/g, 14.91 mg GAE/g and 89.13%, respectively. No significant difference was observed between predicted and experimental values, indicating good fitness of fitted model and successful application of RSM.