• Korean Journal of Microbiology
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• v.37 no.1
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• pp.61-65
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• 2001

Epifluorescence microscopy was used to observe epiphytic bacteria directly on plant leaf surfaces as well as indirectly in the leaf liberating solution by staining with fluorochromes of 4',6-diamidino-2-phenylindole (DAPI) and acridine orange(AO). Epiphytic bacteria could not be well observed on the leaf surface by staining with AO due to an intrusive orange or red background fluorescence. However, DAPI gave us clear epifluorescent images of the bacteria on the leaf. On the contrary, epiphytic bacteria in the liberating leaf solution were well observed on filters stained by both types of fluorochrome, although DAPI showed better fluorescent images than AO and not necessarily required a washing step of the filters stained. The optimum conditions of the DAPI stains were 5 $\mu$g/ml for 5 min both for leaves and for filters of the liberating solution. It was confirmed that a critical step in the epifluorescence microscopy of leaf surfaces was to minimize release of water from the leaf. For this, the stained leaf samples were put on a filter paper, kept in a dry oven at $70^{\circ}C$ for 2 min instead of air-drying, and then immediately observed by epifluorescence microscopy. The established technique was applied to enumerate epiphytic bacteria on oak tree leaf surfaces.

• Korean Journal of Environment and Ecology
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• v.32 no.1
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• pp.118-131
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• 2018

The forest vegetation of Juwangsan National Park, which is famous for its towering scenic valleys, was syntaxonomically described. The study adopted the $Z{\ddot{u}}rich$-Montpellier School's method emphasizing a matching between species composition and habitat conditions. A combined cover degree and the r-NCD (relative net contribution degree) were used to determine a performance of 265 plant species listed-up in a total of 52 phytosociological $relev{\acute{e}}s$. Nine plant communities were classified through a series of table manipulations, and their distribution and actual homotoneity($H_{act}$) were analyzed. Syntaxa described were Carex gifuensis-Quercus mongolica community, Athyrium yokoscense-Quercus mongolica communiy, Arisaema amurense-Quercus serrata community, Lespedeza maximowiczii var. tomentella-Quercus variabilis community, Tilia rufa-Quercus dentata community, Carex ciliatomarginata-Carpinus laxiflora community, Aristolochia manshuriensis-Zelkova serrata community, Onoclea orientalis-Fraxinus mandshurica community, and Carex humilis var. nana-Pinus densiflora community. A zonal distribution was reviewed and the altitude of about 700 m was the transition zone between the cool-temperate central montane zone (Lindero-Quercenion mongolicae region) and southern submontane zone (Callicarpo-Quercenion serratae region). Only 19 taxa were associated with r-NCD 10% or more, most of which were tree species occurring in the Lindero-Quercenion and some of which was a member of open forests. Species composition of forest vegetation was much less homogeneous, showing the lowest $H_{act}$. Nearly natural forests and/or secondary forests in the Juwangsan National Park were defined as a regional vegetation type, which reflects much stronger continental climate in the Daegu regional bioclimatic subdistrict, rhyolitic tuff predominant, and wildfire interference.

• Journal of Forest and Environmental Science
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• v.13 no.1
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• pp.28-50
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• 1997

This study was carried out in a part of conservative plans recovering natural ecosystem which will be submerged, as Hoengseong dam is being constructed around Hoengseong-Eup and Gabcheon-Myun. The submerged area was divided into 7 small areas to figure out what kinds of species of plants are and will be according to detailed geographic characteristics. In the center of the subdivided areas, Degree of Green Naturality, standing biomass, net 36production per year, and composition of plant species were investigated. The results are as following; 1. The surveyed area was classified into 8 degree by Degree of Green Naturality, and the appeared degree and the rate of occupation were 0(3.1%), 1(5.3%), 2(28.2%), 3(0.1%), 5(0.2%), 7(4.6%), 7(44.7%), and 8(13.8%). 2. Standing biomass was 88,436.3 ton/year and net production per year was 12,960.3 ton/year. 3. The list of vascular plants identified in this study consisted of 86 families, 221 genera, 1 variety, 3 formae and 306 species, totally summiting upto 310 species. 4. The 7 surveyed areas were largely occupied by Pinus community. In addition, there were Quercus mongolica, Quercus dentata, and Quercus acutissima communities.

• The Korean Journal of Ecology
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• v.28 no.1
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• pp.15-23
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• 2005

The black locust (Robinia pseudoacacia L.) forests were studied by a phytosociological approach. Particular attention was given to characterize the vegetation classification, distribution pattern, and ecological flora of the syntaxa classified. A total of 38 releves were analyzed by using Correlation coefficient, UPGMA as the clustering method, and Principal Coordinates Analysis for ordination. Ecological flora analyzed by plant character sets such as scrambler, annual and biennial plants, forest elements, and actual urbanization index. The analyzed data are based on site-releve matrix with relative net contribution degree (r-NCD) of species. A total of 77 families, 193 genera and 323 species of vascular plants are recorded. Camellino-Robinietum pseudoacaciae ass. nov. and Phragmites-Robinia pseudoacacia community were described. Main cluster and ordination could be separated: 1) urban type, 2) rural type, 3) riparian type, and 4) combined type. It is defined that the Robinietum is a representative unit on the black locust afforestation, Phragmites-Robinia community on the lentic zone in the river ecosystem, and Cameliino-Robinietum ailanthetosum altissimae as an urban forest type. The Robinietum was considered as a perpetual community.

• Food Science and Preservation
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• v.21 no.4
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• pp.529-535
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• 2014

To examine the levels of preservatives that occur naturally in food, wild plants used as commercial teas, rice cakes, or spices were studied according to the method of the Korean Food Code and analyzed with a gas chromatograph and HPLC. The levels of the natural preservatives (sodium dehydroacetate, sorbic acid, benzoic acid, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, isopropyl p-hydroxybenzoate, propyl p-hydroxybenzoate, isobutyl p-hydroxybenzoate, butyl p-hydroxybenzoate, and propionic acid) in 21 cases were investigated against 15 kinds of wild plants. Six of 15 kinds of wild plants, such as pine needles, bamboo leaf, kudzu leaf, ramie leaf, mugwort, and nut pine leaf, were confirmed to have had sorbic acid, benzoic acid, and propionic acid. 8.201-21.839 mg/kg of benzoic acid was detected in the bamboo leaf, ramie leaf, pine needles, mugwort, kudzu leaf, and nut pine leaf. The sorbic acid contents of the bamboo leaf and the kudzu leaf were 5.630-24.995 mg/kg, respectively. The propionic acid content of the ramie leaf was 61.324-62.726 mg/kg. Nine kinds of wild plants, such as the Korean berchimia leaf, taro leaf, sasa borealis, lotus leaf, kuansh, chrysanthemum zawadskii, oak tree leaf, Chinese pepper leaf, and persimmon leaf, were not detected at the levels of the natural preservatives.

• Korean Journal of Plant Taxonomy
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• v.39 no.4
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• pp.254-263
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• 2009

The distribution and habitat characteristics of Echinosophora koreensis (Nakai) Nakai were investigated to compile basic data for conservation and restoration. The species Echinosophora koreensis is distributed within fourteen regions of the Korean Province of Gangwon, including Yanggu-gun, Inje-gun, Cheorwon-gun, Chuncheon-si and Hongcheon-gun. Natural habitats were located at altitudes of 192-626 m, with inclinations of $1-45^{\circ}$. One hundred and fifty seven vascular plant taxa were identified from 42 quadrats in 14 habitats. Dominant species among the woody plants, based on importance value, were Pinus densiflora and Quercus dentata in the tree (T1) layer, Quercus variabilis, Pinus densiflora and Quercus mongolica in subtree (T2) layer, and Fraxinus rhynchophylla, Prunus sargentii, Zanthoxylum schinifilium, and Ulmus davidiana var. japonica in the shrub (S) layer. Importance values for members of the herb layer were: Echinosophora koreensis 28.34%; Polygonatum odoratum var. pluriflorum 10.21%; Spodiopogon sibiricus 7.60%; Atractylodes ovata 4.77% and Carex lanceolata 4.13%. The importance values of the last four species were high, so they were at affinity with Echinosophora koreensis in their habitats. Average species diversity was 1.03, and evenness and dominance were found to be 0.82 and 0.16, respectively. The soil types were sandy loam and loam. Average field capacity was 13.28%, and the organic matter and soil pH were 6.70% and 5.77, respectively.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.38 no.2
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• pp.77-94
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• 2020

Gugok poems were an important material for studying the Gugok landscape garden(九曲園林). the landscape feature(景物)that emerges from poetry is an important material that expresses the sense of placefulness and garden consciousness(園林意識). However, many studies are passive about plant materials that can explore the perception of nature or the aesthetic embodiment process. Therefore, this study seeks to discover various symbolic meanings and reveal the context of plants that have appeared in Gugok poems in order to expand the research material of the Gugok culture. To carry out the purpose of the study, I collected a total of 25 related poems from 18 Gugoks and found a total of 20 species of trees. I used 'R-program' to derive the meaning of trees and examined the meaning of trees by intertextuality. According to the study, the 20 species of trees contained symbolic meanings of world of Taoist hermit, pursuit of study, constancy, true pleasure, dignity, honest poverty, reign of peace, nostalgia. Many species focused on the symbolism of the 'world of Taoist hermit' and then on the 'dignity' was the most frequent. A number of species, except for the peach, zelkova and oak, had multiple meanings. Among them, pine trees and lotus had a wide range of symbolic meanings and different meanings depending on the characteristics of the surrounding landscape. While the Gugok culture generally targets natural scenery, Yongsan, Toegye, Deoksan, and Jusan showed the characteristics of strengthening or reproducing symbolic meanings through artificial plating and gardening. In order to overcome the limitations of the peach tree, which symbolizes 'Mureungdowon(武陵桃園)', Gugok poems used maple trees and reeds as alternative species. In accordance with the above context, the trees appearing in the Gugok poems expressed their symbolic meaning differently according to the landscape features, acts and purposes of the Gugoks, rather than sticking to the traditional meaning.

• Korean Journal of Ecology and Environment
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• v.48 no.2
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• pp.122-128
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• 2015

This study aims to figure out the vegetation status and characteristics of the spatial distribution in Taeanhaean National Park. As a result, vegetation communities were Pinus thunbergii community, Pinus densiflora community, Pinus thunbergii-Pinus densiflora community, Camellia japonica community, Carpinus turczaninowii community, Carpinus turczaninowii-Pinus thunbergii community, Kalopanax septemlobus community, Koelreuteria paniculata community, Robinia pseudoacacia community, and sand dune and coastal wetland vegetation including Vitex rotundifolia community, Elymus mollis community, Calamagrostis epigeios community, Ischaemum anthephoroides community, Zoysia sinica community, Suaeda maritima community, and Phragmites communis community. Landscape types in actual vegetation map were Pinus thunbergii community (3.92%), Pinus densiflora community (1.40%), Robinia pseudoacacia community (0.05%), sand dune and coastal wetland vegetation (0.11%), field (0.46%), seashore (0.24%), thinning area (0.08%), bareground (0.16%), and sea (93.58%). Area of Pinus thunbergii community among vegetation type was $14.797km^2$ (3.92%) and the largest. Stratification structure and species composition of Pinus thunbergii community distributed in the seashore were different as a result of disturbance and human use. On the other hand, we assumed that succession will take place to the potential natural vegetation that is Quercus serrata and Quercus dentata in undisturbed Pinus thunbergii community. Meanwhile, Erechtites hieracifolia was occupied in understory of Pinus thunbergii community around the seashore and Diodia teres was invaded to sand dune vegetation. They may affect on natural species negatively. Therefore, monitoring and management plans are necessary.

• Korean Journal of Plant Resources
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• v.20 no.5
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• pp.451-460
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• 2007

This study was carried out to construct the data base for biodiversity conservation, and offer the raw data for obtaining the genetic resources. The vascular plants were summarized as 306 taxa; 79 families, 199 genera, 266 species, 35 varieties and 5 forma. The Korean endemic plants were 7 taxa; Salix caprea, S. purpurea var. japonica, Deuitzia coreana, Akebia quinata var. plyphylla, Spiraea prunifolia for. simpiciflora, Lonicera subsessilis and Weigela subsessilis. The naturalized plants were 16 taxa; Dactylis glomerata, Rumex acetocella, R. crispus, Phytolacca esculenta, Tlaspi arvense, Robinia pseudo-acacia, Trifolium repens, Ailanthus altissima, Oenothera lamarckiana, Veronica persica, Ambrosia artemisiifolia var. elatior, Erigeron annuus, Erigeron canadensis, Bidens frondosa, Taraxacum officinale and Sonchus asper. The specific plants by floristic region were 26 taxa; Chloranthus japonica, Populus davidiana, and so forth.

• Journal of the Korean Wood Science and Technology
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• v.49 no.5
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• pp.462-470
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• 2021

To find out the wood species used in national wood structures in the late Joseon Dynasty, 11 volumes of Yeonggeon-Uigwes were reviewed. It was confirmed that the wood was mainly used for the repair and restoration of palaces, shrines, and fortresses. In the 17th to 20th centuries, the wood species specified in Yeonggeon-Uigwes have revealed either hardwood or softwood. As hardwood species, Juglans mandshurica, Zelkova serrata, Fraxinus rhynchophylla, Betula schmidtii, Paulownia coreana, or Quercus spp. (Quercus aliena; Q, variabilis; Q, dentata; Q, acutissima; Q, mongolica; Q, serrata) were used. As softwood species, Pinus densiflora and Pinus koraiensis were used. Investigation of the wood species is important part because the same species is required as a principle when reparing cultural heritage. In this study, the anatomical images of the wood species were investigated for some samples which were taken from the same species that has been stored in the wood specimen room of the National Institute of Forest Science, instead of the actual wood material used. It was possible to find out the wood species of each member in the wooden cultural heritage buildings by reviewing the Yeonggeon-Uigwes in the late Joseon Dynasty, and the anatomical images of the wood species required for determining the wood species in the repair or restoration of the buildings.