• Proceedings of the Korean Information Science Society Conference
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• 2004.04b
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• pp.316-318
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• 2004

DNA 컴퓨팅에 대한 연구가 진행되어 강에 따라 기존의 튜링 머신과 동등한 계산 능력을 가진 다양한 계산 모델이 제안되고 있으며, 이와 함께 DNA의 병렬성을 이용하여 NP 문제들을 풀고자 하는 시도가 계속되고 있다. 그러나 전통적인 폰 노이만 기계에서의 알고리즘이 해집합에 대한 순차적 탐색을 하는 것과는 달리 가능한 모든 해를 미리 생성해 놓고 그 중에서 해를 찾아내는 기존의 DNA 컴퓨팅 알고리즘으로는 NP 문제의 크기가 증가함에 따라 초기 해의 생성조차도 불가능하게 된다. 이에 대한 해법의 하나로 진화적인 방법론을 생각할 수 있지만, 이 경우에는 진화 연산을 위한 추가적인 연산자의 고안과 이의 적용에 따른 어려움이 생긴다. 따라서 본 논문에서는 DNA 컴퓨팅에서 가능한 초기 해를 모두 생성할 수 있는지를 열역학적인 데이터에 근거한 시뮬레이션을 통하여 검증하였다. 이러한 과정을 통해서 값비싼 실제 실험의 성공 여부나 실험 디자인의 정당성 등을 미리 예측할 수 있을 뿐만 아니라, DNA 컴퓨팅이 보다 큰 크기의 NP 문제를 해결할 수 있는 가능성을 제공할 수 있다.

• KSBB Journal
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• v.25 no.2
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• pp.199-204
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• 2010

We generated the feasibility of DNA separation in free-solution using genetically engineered repetitive polypeptides as drag-tags. Two different-sized repetitive polypeptides were designed, expressed in E. coli, and purified. They were conjugated to a fluorescently labeled DNA (100 base), and the electrophoretic mobilities of these conjugate molecules were analyzed on a microchip. The results of these studies indicate that genetically engineered repetitive polypeptide is a prominent candidate for rapid and high-throughput genetic mutation detection, such as SNP analysis.

• Proceedings of the Korean Information Science Society Conference
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• 2003.04c
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• pp.365-367
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• 2003

염기서열 디자인은 DNA computing, 샘물정보학 등의 분야에서 실험 설계시 고려해야할 중요한 문제 중의 하나이다. 이 문제는 다양한 조건을 만족시키는 최적의 염기서열 집합을 생성하는 조합 최적화 문제로 생각될 수 있으며, 염기서열이 갖추어야 할 조건을 적합도 함수로 사용한 진화 연산 등의 방법이 적용되어 왔다. 본 논문에서는 여러 논문들에서 제시된 적합도 함수의 구체적인 형태를 해 공간상에서 조사해 보았으며, 각 적합도 함수간의 관계도 분석해 보았다.

• Management & Information Systems Review
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• v.30 no.4
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• pp.293-314
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• 2011

The current study investigated the position of future strategy analyzed from the perspective of design management in relation to 10 strategic industries implemented through 3 steps based on promising growth and advancement of Busan industries and evidence provided from a study on the development program of design industry in Busan. It elucidated the role of design industry as a key role from the perspective of design management in an age of creative revolution of futures values. It analyzed the associations between composition of future strategy and design industry in 10 strategic industries of Busan, and explained the relationships with the strategic industries. The perspective of design management involves that design as a ground of values is an industry of the future values, which performs a key strategic function and role, and a theoretical investigation examined the relationships between main functions of design management and business management. Chapter 3 organized items proposed in the design development program in Busan and examined goals and systems which become basic formation of establishment of design strategy in Busan and conditions for design industry in the associations with strategic industry. Chapter 4 described priorities of practicability by step through analyzing and grouping top 30 projects in Busan industry including meanings as key strategy, position relations, and policy priorities by analyzing elements of design management of strategic industry and describing and analyzing the concept of promoting Busan design. The theme of the present study is to change perception of design management as a key value and a condition to decide creativity industry into future industry and to evaluate vision of Busan design industry and meanings proposed as proceeding strategy. The early 21st century is an age when agrarian society has changed into industrial society is dominated by knowledge economy of the information revolution and one should prepare for the growth phase of creative innovation based on creative revolution of the 4th wave of creative society by design management which has become a center in 2000s on the whole. With the advent of creative paradigm and based on the function and role of the current creative economy age new innovation DNA of design management will be created. Design process has changed through information and knowledge-oriented trends of digital through convergence between industries from industrial design to convergence of industries, and it is expected that integrated design of value creation using information and technology will play a key role in Busan design industry development and top 10 strategic industries.

• Proceedings of the Korea Information Processing Society Conference
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• 2003.11b
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• pp.733-736
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• 2003

본 논문은 DNA microarray chip 을 사용한 실험 결과로 생산되는 대량의 데이터를 효율적으로 관리하기 위한 LIMS 개발에 대해 기술한다. 기존의 상용 LIMS 는 보편적 패턴과 방식을 정규화하여 제공하기 때문에 실험실의 고유한 방식을 포함하긴 어렵다. 본 논문에서는 유연성 있는 LIMS 를 개발하기 위해 특정 실험 중심으로 설계하면서 MAGE-OM 의 표준을 따르도록 디자인하였고, HYLIMS manager 라는 Local Application 과 검색을 주로 이용하는 사용자를 위하여 Web 검색 시스템을 구현하였다. 데이터베이스의 부하를 줄이기 위해 데이터 저장용 DB 와 검색용 DB 를 구분하였고, 데이터를 타입과 처리 형태에 따라 분류하여 관리하였으며 데이터 보안을 위해 실험 관리자가 사용자의 접근 제한을 설정 할 수 있도록 하였다.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.24 no.6
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• pp.133-144
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• 2021

This study aims to estimate location of land mammals habitat by analyzing spatial data and investigate how to apply environmental DNA monitoring methodology to lotic system in Yangpyeong-gun, Gyeonggi-do. Environmental DNA sampling points are selected through spatial analysis with QGIS open source program by overlaying Kernel density of wild boar(Sus scrofa), elevation, slope and land-cover map, and 81 samples are collected. After 240 mL of water was filtered in each sample, metabarcoding technique using MiMammal universal primer was applied in order to get a whole list of mammal species whose DNA particles contained in filtered water. 8 and 22 samples showed DNA of wild boar and water deer, respectively. DNA of raccoon dog, Eurasian otter, and Siberian weasel are also detected through metabarcoding analysis. This study is valuable that conducted in outdoor lotic system. The study suggests a new wildlife monitoring methodology integrating overlayed geographic data and environmental DNA.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2004.11a
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• pp.21-28
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• 2004

모든 생명체의 genetic information에는 보존적 염기서열과 다형적 염기서열이 존재한다. 다형적 염기서열과 보존적 염기서열은 하나의 종(species)을 감별하거나, 여러 종류의 종을 동시에 감별할 수 있는 genotyping의 표지자로 각각 이용될 수 있다. 본 논문은 병원성 감염질환 세균, 식중독 유발 세균, 생물의약품 오염 유발 세균 및 환경오염 세균 등 세균의 존재 유무와 속과 종 감별을 위해 대부분 세균 종의 보존적 염기서열과 다형적인 염기서열을 포함하고 있는 23S rDNA 유전자의 표적 염기 서열로부터 고안된 세균 특이적(bacterial-specific), 속 특이적(genus-specific), 종 특이적(species-specific) 올리고 뉴클레오티드프로브와 프라이머를 디자인하는 시스템을 소개한다. 시스템을 통해서 얻어진 프로브와 프라이머들은 PCR을 통한 검증단계를 거쳐서 디자인 결과의 정확성을 확인하였다. 본 시스템의 이용으로 프로브와 프라이머를 디자인하는데 몇 주가 소요되는 시간을 몇 일 내로 줄일 수 있었으며, 체계적인 데이터의 관리로 결과의 정확성을 높일 수 있었다.

• Journal of Environmental Impact Assessment
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• v.32 no.2
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• pp.94-111
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• 2023

In order to establish an effective management strategy for invasive species early detection and regular monitoring are required to assess their introduction or dispersal. Environmental DNA (eDNA) is actively applied to evaluate the fauna including the presence of invasive species as it has high detection sensitivity and can detect multiple species simultaneously. In Korea, the applicability evaluation of metabarcoding is being conducted mainly on fish, and research on other taxa is insufficient. Therefore, this study identified the feasibility of detecting invasive species in Korea using eDNA metabarcoding. In addition, to confirm the possibility of detection by taxa, the detection of target species was evaluated using four universal primers (MiFish, MiMammal, Mibird, Amp16S) designed for fish, mammals, birds, and amphibians. As a result, target species (Trachemys scripta, 3 sites; Cervus nippon, 3 sites; Micropterus salmoides, 7 sites; Rana catesbeiana, 4 sites) were detected in 17 of the total 55 sites. Even in the selection of dense sampling sites within the study area, there was a difference in the detection result by reflecting the ecological characteristics of the target species. A comparison of community structures (species richness, abundance and diversity) based on the presence of invasive species focused on M.salmoides and T.scripta, showed higher diversity at the point where invasive species were detected. Also, 1 to 4 more species were detected and abundance was also up to 1.7 times higher. The results of invasive species detection through metabarcoding and the comparison of community structures indicate that the accumulation of large amounts of monitoring data through eDNA can be efficiently utilized for multidimensional ecosystem evaluation. In addition, it suggested that eDNA can be used as major data for evaluation and prediction, such as tracking biological changes caused by artificial and natural factors and environmental impact assessment.

• Journal of Life Science
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• v.25 no.8
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• pp.903-909
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• 2015

Loop-mediated isothermal amplification (LAMP), a PCR-based diagnostic method, is based on autocycling strand displacement DNA synthesis in the presence of exonuclease-negative Bst DNA polymerase under isothermal conditions. With the help of four specific primers that recognize six different sequences of a target DNA, LAMP has high specificity in pathogenic identification in a short time. Hence, in the present study, LAMP is used as a diagnostic tool in the identification of the most dreadful aquatic pathogenic species, Vibrio alginolyticus, and to develop species-specific LAMP primers and optimization of LAMP reaction conditions such as annealing temperature, elongation time, and other PCR chemical concentrations, including MgSO₄, dNTPs, Betaine, and Bst polymerase. The optimized LAMP primers were also checked for specificity with other Vibrio species, which showed that the designed primers were very specific to V. alginolyticus After the first introduction of a species name like this one, the first part (“Vibrio” in this case) should be abbreviated to only the first letter.only. These are usually the most harmful pathogens of the Vibrio species that appear in shrimp and crabs. The results also revealed that the LAMP assay could be 10-fold more sensitive than conventional PCR in detecting V. alginolyticus. This could be the first report on using a rapid and highly sensitive technique, the LAMP assay, in the effective diagnosis of the pathogenic bacteria V. alginolyticus, which could help in the early detection of diseases, particularly in aquaculture.

• The Korean Journal of Mycology
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• v.38 no.1
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• pp.34-39
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• 2010

We have attempted to verify 30 strains of Hypsizigus marmoreus from various mushroom stocks in Korea using random amplified polymorphic DNA (RAPD) methodology. Chromosomal DNAs of them were extracted and subjected to PCR analyses with 3 random primers. Each PCR produced approximately 30 distinct PCR bands with the size from 200 bp to 3000 bp. A dendrogram was acquired using the unweighted pair-group method with arithmetic average (UPGMA) clustering methodology on the basis of the DNA band pattern. The analysis revealed that 30 strains of H. marmoreus were clustered into two distinct clusters. Cluster 1 contained 3 subgroups while the cluster 2 consisted of rather diverse strains. Interestingly, Hm3-10, a wild strain collected from Deog-Yu mountain, was not included in either clusters, indicative of uniqueness of this strain. We nextly attempted to develop strain-specific DNA markers to verify a specific strain. A unique band in the RAPD gel lane of Hm0-4 was extracted and its sequence was determined. PCR with a primer set from the determined sequence revealed that the primer set gave a 250 bp DNA band only for Hm0-4, indicating that this approach works well for the strain-specific identification of H. marmoreus.