• Transactions of the Korean Society of Mechanical Engineers
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• v.16 no.1
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• pp.1-12
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• 1992

Generally, laser machines with high generated power can be developed by means of enhancing their mean power, since the enhancement of mean power exerts an influence on peripheral parameters and machining performance. In this research, we evaluate the various machining properties by the use of two machines which bear different mean power each other, so that we may study various effects of the enhancement of mean power. As a result, when the mean power comes to be enhanced to 75%, we obtain the increase of output energy up to 69% and of peak power more than 95%, and also obtain almost twice of the cutting speed. Moreover we find the fact that if the test pieces have enough thickness in contrast with output energy, the pulse frequency moves toward the frequency bandwidth which takes proportion to the cutting speed mas well as to the amount of material removal per unit time. In addition it is finally obtained that the laser machine with high output power yields small taper degrees at kerf parts, while it has large cutting widiths and dross lengths.

• Transactions of the Korean Society of Mechanical Engineers
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• v.16 no.10
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• pp.1940-1954
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• 1992

Fine grid computations were attempted to analyze the turbulent flows in the near wall low Reynolds number region and the numerical analyses were incorporated by a finite-volume discretization with full find grid system and low Reynolds number k-.epsilon. model was employed in this region. For the improvement of low Reynolds number k-.epsilon. model, modification coefficient of eddy viscosity $f_{\mu}$ was derived as a function of turbulent Reynolds number $R_{+}$ and nondimensional length $y^{+}$ from the concept of two length scales of dissipation rate of turbulent kinetic energy. The modification coefficient $f_{\epsilon}$ in .epsilon. transport equation was also derived theoretically. In the turbulent kinetic energy equation, pressure diffusion term was added in order to consider low Reynolds number region effect. The main characteristics of this low Reynolds number k-.epsilon. model were founded as : (1) In high Reynolds number region, the present model has limiting behavior which approaches to the high Reynolds number model. (2) Present low Reynolds number k-.epsilon. model dose not need additional empirical constants for the transport equations of turbulent kinetic energy and dissipation of turbulent kinetic energy in order to consider wall effect. Present low Reynolds number turbulence model was tested in the pipe flow and obtained improved results in velocity profiles and Reynolds stress distributions compared with those from other k-.epsilon. models.s.s.

• Microbiology and Biotechnology Letters
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• v.36 no.3
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• pp.182-188
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• 2008

A hyperthermophilic bacteria (strain HJ6) was isolated from a hot springs located in the Arima-cho, Hyogo, Japan. The cells were long-rod type ($2-4{\mu}m$), about $0.4{\mu}m$ in diameter. The pH and temperature for optimal growth were 6.5 and $80^{\circ}C$, respectively. Phylogenetic analysis based on the 16S rDNA sequence and biochemical studies indicated that HJ6 belonged to the genus Thermus thermophilus (Tt). The gene encoding the Trehalose synthase (TS) was cloned and sequenced. The open reading frame (ORF) of the TtTS gene was composed of 2,898 nucleotides and encoded a protein (975 amino acids) with a predicted molecular weight of 110.56 kDa. The deduced amino acid sequence of TtTS showed 99% and 83% identities to the Thermus caldophilus TS and Meiothermus ruber TS, respectively. TtTS gene was expressed in Escherichia coli cells, and the recombinant protein was purified to homogeneity. The optimal temperature and pH for Trehalose synthase activity were found to be $80^{\circ}C$ and 7.5, respectively. The half-life of heat inactivation was about 40 min at $90^{\circ}C$. The maximum trehalose conversion rate of maltose into trehalose by the enzyme increased as the substrate concentration increased, and reached 55.7% at the maltose concentration of 500 mM, implying that the enzyme conversion was dependent of the substrate concentration.