• The Korea Swine Journal
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• v.17 no.8 s.192
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• pp.72-73
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• 1995

• Korean Journal of Veterinary Research
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• v.28 no.2
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• pp.365-369
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• 1988

The present study was done to demonstrate ADV antigens in frozen and paraffin sections from ADV-infected pigs and cell cultures by using of the IGS method. Tissue specimens from 3 young pigs infected with ADV-phylaxia strain and of 2 healthy pigs were used. Fibroblastic cells originated from pig brain and BHK cells were grown and confluent monolayers were infected with the virus. Two monoclonal antibodies and a specific hyperimmune serum to ADV were used as the source of primary antibodies for both the IGS and immunoperoxidase methods. Application of the IGS method yielded a black fine granular reaction in positive areas, and the results were superior to those obtained using the immunoperoxidase technique for all cases tested. The IGS method might be useful in the detection of various viral antigens in tissue sections.

• Korean Journal of Veterinary Research
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• v.32 no.3
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• pp.365-368
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• 1992

1988년부터 1990년 6월까지 전국의 양돈장에서 수집된 돼지 유사산 태아 74복에서 바이러스성 원인체 분리 및 혈청학적 진단을 수행하였던 바 다음과 같은 결과를 얻었다. 공시한 74복의 유사산 태아중 44복의 태아 흉강액에서 면역 globulin이 검출되어 전염성 질병감염에 의한 유사산으로 추정되었다. 이중 37%가 바이러스성 유사산으로 나타났으며 유사산의 원인체별 분포를 살펴보면 돼지 파보바이러스가 21%로 가장 높았으며, 뇌심근염 바이러스가 11%, 일본뇌염 바이러스가 9% 등의 순으로 나타났다. 한편 돼지 콜레라바이러스 및 오제스키병 바이러스에 의한 유사산이 각각 1건씩 검출되었으며 동일 유사산 태아에서 2가지 병원체가 중복감염된 예도 관찰되었다.

• 사료
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• s.7
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• pp.4-28
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• 2004

태국등동남아시아국가에서 시발된가금인플루엔자가 2003년 12월국내에서처음으로발생되었다. 가금인플루엔자의발생은 비단양계산물뿐만 아니라전체축산물의소비를급감시키며 우리축산업의 생산기반을크게위축시키는결과를 가져왔다. 이는가축질병에 대한 잘못된이해와언론의 그릇된보도의 탓도있으나결국 가장큰원인은 소비자들의축산물 안전성에대한요구를수용치못한 결과이다. 향후 축산물 안전성에 대한소비자들의 욕구는 더욱증대될것이며, 이를 충족시키지못하는 경우축산물에대한소비자들의기피현상은 보다 근본적이고 장기적인 경향으로나타날 수있다. 기히수입개방되어 있는 축산업시장에칠레와의자유무역협정을시작으로 축산물에 대한 관세철폐가현실로다가온시점에서우리 축산물의시장개혁을 위해서는 외국축산물과의차별화 즉, 소비자들의가장큰관심사인 축산물안전성을제고시키는것이다. 이에 국내에서주로발생하거나재발가능성이 높은주요가축질병에 대한증상 및 예방대책등을 알아본다.

• Korean Journal of Veterinary Research
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• v.37 no.4
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• pp.765-772
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• 1997

제주지역 돼지에서 각종 전염성 질병 원인체에 대한 항체를 조사하여 그간 전염성 병원체에 대한 역학조사가 미진하였던 부분을 보완하여 질병의 분포를 파악하고자 1995년부터 1996년에 걸쳐 제주도 전역에서 돼지의 혈청을 채취하여 각종 병원체에 대한 항체 분포율을 조사하였다. 본 연구에서 검사한 돼지 혈청 시료에서는 돼지 오제스키병 바이러스에 대한 항체는 전혀 검출되지 않았다. 돼지 콜레라바이러스에 대한 항체는 기대 수준 이하로 낮아 백신접종이 원활히 수행되고 있지 않음을 시사하였으며 특히 농장에 따라 항체 보유돈과 항체 음성돈이 혼재하는 농장과 항체가 전혀 검출되지 않는 농장 등 돼지 콜레라 방역의 사각지대가 존재할 가능성이 있음을 보여주었다. 유 사산 원인체인 돼지 파보바이러스 및 뇌심근염에 대한 항체가가 다양하게 나타나 일부 문제가 있을 것으로 사료되었다. 돼지 생식기호흡기증후군(PRRS) 바이러스에 대한 항체 분포율은 내륙 보다 다소 낮게 나타났고, 돼지 influenza virus, 위축성 비염, 흉막 폐염 등 각종 세균성 질환에 대한 항체수준도 다양하게 나타났다. 본 혈청학적인 연구결과는 제주지역에서의 양돈방역 정책수립 및 질병방제의 기초자료로 유용하게 이용될 것으로 사료된다.

• Korean Journal of Veterinary Research
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• v.34 no.1
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• pp.77-83
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• 1994

Three viral strains, causing CPE in porcine alveolar macrophage cell, were isolated from aborted fetus, serum from young pig showing blue-ear sign and lung of suspected pig, respectively. The differential diagnostic results showed no characteristics of Aujeszky's disease virus(ADV), hog cholera virus (HCV), Japanese encephalitis virus(JEV), porcine parvovirus(PPV) and encephalomyocarditis virus (EMCV). However, positive reactions were demonstrated by IFA using monospecific porcine antibodies against Lelystad virus. When the paired sera of experimentally inoculated swine with one of isolate, KPRRSV-l were tested by IPMA, the result indicated that the isolate was related to United States isolate than European LV.

• Korean Journal of Veterinary Research
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• v.28 no.1
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• pp.99-103
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• 1988

The first outbreak of Aujeszky's disease(AD) was identified from piggery located at the southern part of Korea in July, 1987. This piggery suffered from a significant economic loss caused by unexpected piglet mortality and reproductive failure. Etiologic viral agents were isolated from tonsil and spleen of the infected piglets, and the isolates produced a typical cytopathic effects of herpesvirus with giant cell formation when inoculated in many different cells. Subsequently the field isolates were characterized as suid herpesvirus I by cross-neutralization test and indirect fluorescence assay utilizing specific monoclonal antibody, and were proved to be a pathogenic strain of AD virus(ADV).

• Korean Journal of Veterinary Research
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• v.33 no.2
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• pp.241-247
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• 1993

Forty day old piglets were intranasally inoculated with 2ml of Aujeszky's disease virus (NYJ-1-87 strain, $10^{7.0}$ $TCID_{50/0.2}ml$), and the viral antigens were detected in nasal and circulating white blood cells for 20 days after inoculation by immunocytochemical method. Antibody titers in the blood were also detected by neutralizing test and Aujeszky's disease serodiagnostic kit(Choong Ang) in this periods. 1. Viral antigens were detected by the immunocytochemical technigue, and positive reactions were observated in nasal cells from the 2nd to the l0th days after inoculation and circulated white blood cells from the 4th to the 12th days after inoculation. 2. In neutralization test antibodies levels showed titers of 2 on the 8th day, 8 on the l5th day, 16 on the 18th day and 32 on the 20th day after inoculation. In serodiagnostic kit test positive reactions were observed after the 15th day after inoculation.

• Korean Journal of Veterinary Research
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• v.36 no.2
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• pp.359-369
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• 1996

An effective candidate subunit vaccine was prepared by using the immunostimulating complexs(ISCOMs) with Quil A and recombinant protein(gp50, gIII and inactive $\alpha$-ADV) Aujeszky's disease virus(ADV). The weaned pigs were twice immunized with a ADV-ISCOMs, and followed by intramuscular challenge with $1{\times}10^4$ $TCID_{50}$ ADV(strain Yangsan). The unvaccinated pigs were also challenged with same dose of ADV. At 5 days after challenge, the control pigs have developed ADV clinical signs. Whereas, the vaccinated pigs protected them from ADV-induced acute symptoms and death. Also, to identify the lymphocyte subpopulation in peripheral blood with pigs from ADV-ISCOMs vaccinated and control group, lymphocyte reacted with a panel of monoclonal antibodies which are specific to swine leukocyte surface antigens and assayed by the flow cytometry. MHC class I, CD2, CD8, N cells, CD11a, and CD45 antigen positive cells were decreased after inoculating virulent ADV Yangsan strain in control group. The data indicated that ISCOMs technique was useful in ADV subunit vaccine preparation and demonstrated the importance of gp50, gIII as a component of ADV vaccine.

• Korean Journal of Veterinary Research
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• v.47 no.4
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• pp.417-423
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• 2007

Serological surveillance programs in animal populations are becoming increasingly important to estimate prevalence of a specific disease and subsequently to document disease-free status in a region or a country. For these purposes, the programs need to be based on both theoretical and economical aspects from the designing phase. From Aujeszky's disease (AD)-eradication program point of view, group of animals (aggregates, herds) not individual animal is the more important sampling unit of concern. In this study the authors therefore attempted to compute an appropriate sample size tailored to a current surveillance program against AD, assuming that the goal of this program is either herd-level prevalence estimation or documentation of AD-freedom. For prevalence estimation, assuming a finite population with imperfect sensitivity (Se) and specificity (Sp) of ELISA kit for AD diagnosis, the number of herds present, expected herd prevalence, and desired accuracy for a certain level of confidence, sample size was estimated at herd-level in the first stage and individual animal-level in the second stage. A two-stage sampling design was used to calculate a sample size to indicate AD-freedom. In this instance, the computation was based on the possible detection of a predetermined prevalence at a certain herd-level Se and Sp. This study indicated that the sample size varied with predetermined confidence, tolerance, Se and Sp at herd- and animal-level, and within- and among-herd prevalence. In general, smaller sample size was required to estimate AD prevalence than to document of AD-freedom. Compared to individual-based samples, two-stage sampling strategy requires a larger sample size to show disease-freedom. Statistical considerations including herd-level test characteristics when designing surveillance program also are further discussed.