• Radiation Oncology Journal
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• v.19 no.1
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• pp.45-52
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• 2001

Purpose : Granulocyle-colony stimulating factor (G-CSF) has been widely used to treat neutropenia caused by chemotherapy or radiotherapy. The efficacy of recombinant human hematopoietic growth factors in improving oral mucositis after chemotherapy or radiotherapy has been recently demonstrated in some clinical studies. This study was designed to determine whether G-CSF can modify the radiation injury of the intestinal mucosa in mice. Materials and Methods : One hundred and five BALB/c mice weighing 20 grams were divided into nine subgroups including G-CSF alone group $(I:10\;{\mu}g/kg\;or\;II:100\;{\mu}g/kg)$, radiation alone group (7.5 or 12 Gy on the whole body), combination group with G-CSF and radiation (G-CSF I or II plus 7.5 Gy, G-CSF I or II plus 12 Gy), and control group. Radiation was administered with a 6 MV linear accelerator (Mevatron Siemens) with a dose rate of 3 Gy/min on day 0. G-CSF was injected subcutaneously for 3 days, once a day, from day -2 to day 0. Each group was sacrificed on the day 1, day 3, and day 7. The mucosal changes of jejunum were evaluated microscopically by crypt count per circumference, villi length, and histologic damage grading. Results : In both G-CSF I and II groups, crypt counts, villi length, and histologic damage scores were not significantly different from those of the control one (p>0.05). The 7.5 Gy and 12 Gy radiation alone groups showed significantly lower crypt counts and higher histologic damage scores compared with those of control one (p<0.05). The groups exposed to 7.5 Gy radiation plus G-CSF I or II showed significantly higher crypt counts and lower histologic damage scores on the day 3, and lower histologic damage scores on the day 7 compared with those of the 7.5 Gy radiation alone one (p<0.05). The 12 Gy radiation plus G-CSF I or II group did not show significant difference in crypt counts and histologic damage scores compared with those of the 12 Gy radiation alone one (p>0,05). Most of the mice in 12 Gy radiation with or without G-CSF group showed intestinal death within 5 days. Conclusion : These results suggest that G-CSF may protect the jejunal mucosa from the acute radiation damage following within the tolerable ranges of whole body irradiation in mice.

• Journal of Life Science
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• v.20 no.4
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• pp.555-560
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• 2010

The objective of this study was to investigate levels of serum creatinine, CuSOD and MnSOD protein expression in the kidney after renal ischemic reperfusion with pre-exercise using heat shock protein 70.1 in knock-out mice (KO). The C57/BL6 strain (Wild type: WT) and KO were divided into 4 groups as follows: Sham control group (Sham), pre-exercise group (Ex), pre-exercise +ischemia group (Ex+IR), and ischemia group (IR). CuSOD and MnSOD expression were significantly decreased (p<0.01, p<0.05) and blood creatinine concentration was significantly increased (p<0.01) in the IR group of KO. In contrast, CuSOD and MnSOD expression in the Ex+IR group of KO were higher than the IR group, while creatinine concentration was significantly lower. These results suggest that Hsp70 is directly correlated to renal ischemic reperfusion injury. Pre-exercise in renal ischemia might prevent or inhibit positive oxidative stress inhibitory effects by increasing anti-oxidative enzymes (CuSOD, MnSOD) within the kidney and improve to prevent renal function. Thus, pre-exercise may have a protective role against renal injury after renal ischemia.

• Korean Journal of Environment and Ecology
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• v.26 no.3
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• pp.322-332
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• 2012

The objectives of this paper were to investigate home range, habitat selection, and threat factors of leopard cats (Prionailurus bengalensis) living in rural area of Korea. The results based on radio tracking of three leopard cats (two males and one female) can be summarized as follows. First, the average home range of leopard cats were $2.64{\pm}1.99km^2$ (Kernel 95) and $3.69{\pm}1.34km^2$ (MCP 100), and the average size of core areas was $0.64{\pm}0.47km^2$ (Kernel 50). The home range of a male leopard cat that radio-tracked in winter was the largest ($5.19km^2$, MCP 100). Second, the Johnson's habitat selection model based on the Jacobs index showed that leopard cats preferred meadows and paddy fields avoiding forest covers at the second level, whereas they preferred meadows adjacent to streams and avoided paddy fields at the third level. Finally, roadkill could be prime threat factor for the cat population. Therefore, habitats dominated by paddy fields, stream corridors with paved roads, and human settlements with insufficient forest patches could threaten the long-term viability of leopard cat populations. Thus the habitat managements for the leopard cat conservation should focus on the prevention of road-kill and the installation of wildlife passages in rural highways adjacent to stream corridors.

• Korean Journal of Environment and Ecology
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• v.30 no.3
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• pp.328-334
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• 2016

This study identified the habitat use pattern and migration distance of spot-billed ducks during winter that occur for breed and wintering. The study was carried out using GPS-mobile phone based Telemetry (WT-300) to provide the research results as basic data for protection and management of the habitats of water birds in Korea. The tracking devices (WT-300) were attached to a total of twenty spot-billed ducks collected from the rivers and reservoirs in Gyeonggi-do Anseongcheon, Chungcheongnam-do Seocheongun, Jeollabuk-do Mangyunggang, Dongjingang, and Jeollanam-do Haenamgun. It was found that spot-billed ducks used rivers most frequently as their habitats (47.3%), followed by rice fields, ocean, inland wetlands, and tidal flats. While spot-billed ducks used rice fields at night time and ocean, inland wetlands, and tidal flatss at day time, they used rivers regardless of time. Change rate of habitat use pattern was highest between 6:00am and 8:00am and between 16:00pm and 18:00pm. The average daily migration distance was $0.75{\pm}0.27km$/2hours and accumulated migration distance was 8.95km. Spot-billed ducks covered the longest migration distance between 6:00am and 8:00am and between 16:00pm and 18:00pm; the distance they moved during the periods from 6:00am to 8:00am and from 16:00pm to 18:00pm was 2km but during the rest of the time, it was less than 1km for equivalent durations. The time when spot-billed ducks covered the longest migration distance also corresponds to the time when they showed the greatest change rate of habitat use pattern.

• Korean Journal of Food Science and Technology
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• v.47 no.6
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• pp.779-784
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• 2015

This study was conducted to investigate the effect of herbs on memory improvement by focusing on their cholinergic functions in Tg2576 mice. Seven herbs were used to obtain extracts by using alcohol and water. In screening test for cholinergic activities of the extracts, acetylcholinesterase (AChE) activity was highly inhibited in Oenanthe javanica alcohol extract (OJAE, 18.76%) as compared with the others. The OJAE-treated Tg2576 (Tg-OJAE) groups showed the statistically significant increases of latency time in passive avoidance test. Also, it was found that the concentration of $A{\beta}1-42$ was significantly reduced in Tg-OJAE groups compared to non-treated Tg2576 groups. In the additional enzyme test, it was found that $IC_{50}$ of OJAE was $991.77{\mu}g/mL$ and OJAE acted as an uncompetitive inhibitor of AChE. Therefore, it seemed that OJAE can be used for the development of processed foods for memory improvement.

• Journal of Preventive Medicine and Public Health
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• v.36 no.4
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• pp.303-313
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• 2003

General information is summarized, that is necessary to introduce a scientific assessment of the human health and exposure issue concerning dioxin and dioxin-like compound. Scientific literatures were reviewed to assess the background exposures to the dioxin-like compounds for normal residents. Epidemiologic studies were also reviewed to assess malignant and nonmalignant sweets of dioxins. In 1997, the International Agency for Research on Cancer classified 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as a human carcinogen, primarily based on occupational cohort studies. The US Environmental Protection Agency made the same decision in it's Draft Dioxin Reassessment. Epidemiologic evidences point to a generalized excess of all cancers, without any pronounced excess at specific sites. Reported non-cancer effects included a range of conditions affecting most systems. Among them, chloracne, elevation in gamma glutamyl transferase(GGT), and alterations in reproductive hormones are related to TCDO, Other adverse outcomes, such as lipid concentrations, diabetes, circulatory and heart diseases, immunologic disorders, neurobehavioral effects, and developmental outcomes require further study before their respective relationships to TCDD can be more definitively assessed.

• Journal of Environmental Impact Assessment
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• v.26 no.5
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• pp.376-384
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• 2017

Bangladesh is a South Asian country with subtropical monsoonal climate between the intersection of the Indo-Himalayan and Indo-Chinese sub-regions, is known as biodiversity hotspot of the Asian region. The country has different types of forest like deciduous forest, evergreen forest, mixed forest, haor (wetlands) and mangrove forest. The natural beauty of the country is increased with the presence of so many rivers, longest sea beach of the world, green plants, critical hilly regions and green agricultural forest widely spread here and there. Sundarbans is the world largest mangrove forest and world natural heritage site declared by UNESCO in 1999 situated in Bangladesh and India. About 62 percent of this mangrove forest is situated in Bangladesh and there are so many plants and animals are found in this forest. To meet the increasing demand of the large population most of the natural ecosystem is now altered, deforestation rate is increased, natural habitat of the species is disturbed. Due to the imbalance of the climate and natural system many of the rare species of the world found this region is now endangered and some of the species are extinct. Directly or indirectly they are benefited from natural resources. At present time community, based ecotourism is also an important source of income for rural poor peoples. To protect the natural resources the government is now developed so many conservation acts and policy as well NGOs are also doing work for the conservation of ecosystem and biodiversity. At present transboundary pollutants and so many natural disasters also destruct the natural resources of Bangladesh.

• Applied Microscopy
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• v.33 no.3
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• pp.233-241
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• 2003

Patterns of tannin accumulation in leaves of C-4 Euphorbia maculata have been examined using electron microscopy. Tannins, which are secondary metabolite phenolic compounds, were found to be deposited conspicuously in vacuoles of certain tissues regardless of their stage in development. However, patterns of deposit accumulation were distinguishable by their cell type during leaf differentiation. The deposits appeared most concentrated in the concentric bundle sheath cells enclosing veins, while little or no density was detected mostly in the mesophyll cells close to the epidermis. An ultrastructural study revealed that the deposits were restricted to the vacuoles at an early stage of leaf development; during which the vacuoles were almost completely filled with the tanniferous substances. The deposits themselves took different forms ranging from granules to huge globules while expanding leaf blade. As the leaf matured, the deposits accumulated either centripetally adjacent to the inner tangential tonoplast or by penetration into the cytoplasm amongst various cellular organelles, resulting in an extremely dense cytoplasm. Electron micrographs frequently showed the delineation of each organelle by the presence of dense deposits within the cytoplasm. Some large depository vacuoles filled with tannins had a corrugated appearance on the sectioned surface. The pattern and potential role of the deposits have been discussed.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.5
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• pp.12-25
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• 2004

Integration system of conservation ecology, ecosystem management and land planing in landscape scale is major principle strategy for national management system of environmental resource and biodiversity. Current conservation strategy is concerned with not only population and habitat level but also ecosystem and landscape level. In especially, ecological networking to link core areas or major habitat patches is most eminent and emerging issue in European Union (EU) and North America. Moreover, the promotion of national ecological network system in EU is focused on national cooperation to construct ecological corridor for key habitat that distributed in many countries. Integrating landscape ecology into conservation ecology and restoration have important role to stimulate the network system in regional and national level. In this review paper, comprehensive and necessary considerations arisen from the view of landscape ecology were discussed for the present situations of wildlife conservation and management in Korea compared with other countries. Especially, the conservation strategy and policy of biodiversity were addressed in broad sense including habitat protection, legal approaches, and ecological network programs. Finally, a national ecological network system was suggested for environmental policy in Korea in global consideration after the Korean Unification.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.8
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• pp.1246-1251
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• 2004

To investigate the effects of plant extracts on the protection against liver damage by $CCl_4$ in rat, two kinds of experiment were performed, firstly by the primary hepatocyte culture and secondly by the animal feeding. The primary hepatocyte culture with the extracts of pine leaf, soybean sprout and mugwort showed significantly low activities (p<0.01∼0.05) of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), indicating an excellent protective effect against liver damage by $CCl_4$. In the second experiment, the microsomal malondialdehyde (MDA) contents of the above same groups were also significantly lower (p<0.01) than the $CCl_4$-treated group without plant extracts, but shiitake showed less effect. Among four kinds of plant extracts, extracts of pine leaf and mugwort showed also much higher activities of the microsomal cytochrome P-450 in comparison to soybean sprout and shiitake. In the test of xanthine oxidase (XOD) activity, all of three groups except shiitake showed significantly low activities (p<0.01). These consistent results in vitro and in vivo suggest that the extracts of pine leaf, soybean sprout and mugwort may have strong protective effects against liver damage induced by the potential toxicants such as $CCl_4$.