• The Korean Journal of Zoology
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• v.10 no.1
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• pp.21-26
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• 1967

한국산 굴둑거미속에 관해서는 이미 저자의 한사람인 백의 보고(1966)가 있으나 금반 남궁준이 각지의 동굴에서 채집한 표본을 조사한 결과 다음 2 신종을 얻었기에 보고한다. 신종명 Cybaeus mosanensis n. sp. C. whanseunensis n. sp. 전기 백의 보고 중 한국 미기록종으로 기재한 C. nipponicus 는 남궁이 채집한 수컷의 palpus의 형태등에 의하여 이와는 뚜렷이 다른 한 신종으로 판명되었으므로 이에 C. mosanensis 라 명명하여 여기에 다시 기재하였다. 암컷은 이미 전보에서 상세히 기재하였으므로 본보에서는 수컷만 취급하였다. 따라서 본보이전에 저자들이 보고한 C.nipponicus 는 모두 C. mosanensis 의 synonym 으로 한다. 여기 기재한 2 신종 중 C. mosanensis 는 동굴 밖에서도 많이 발견되지만 C. whanseunensis 는 지금까지 동굴에서만 채집되었다. 이로서 한국산 굴둑거미속은 C. longus Paik, 1966 및 C. mosanensis, C. whanseunensis 의 3 종이 된다.

• Journal of the Speleological Society of Korea
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• no.84
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• pp.10-21
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• 2008

In order to accumulate the basic informations to assess the scientific and natural values of the Hoe-ok Cave located in Mountain Chi-ak, Seong-nam 2 ri, sin-rim myeon, the natural environments in the cave were investigated from November 2004 to June 2005. The results are as follows; The average temperature in the cave was 2.5$^{\circ}C$ in the winter, 5.5$^{\circ}C$ in the spring, 6.8$^{\circ}C$ in the summer. The average humidity in the cave was 82.5％ in the winter, 86.0％ in the spring, 86.0 in the summer. this results show that the temperature was 10$^{\circ}C$ lower, and the humidity was 4$\sim$10％ lower than those in the Go-su Cave. Inside of the Heo-og Cave, the damages from the garbages and smoke stains by the reckless civilians are expanding. It is necessary to execute the research on the geological facts of the cave and the reassessment on its values in the natural history.

• Korean Journal of Environmental Biology
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• v.22 no.1
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• pp.213-219
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• 2004

The bacterial community of water stream, soil and guano in Gossi cave was examined by using PCR amplified the 16S rDNA-denaturing gradient gel electrophoyesis (DGGE). In this study, the genetic diversity and the similarity of bacterial community between open area and non - open area toy cave tour were investigated, and the seasonable variation pattern was compared each other. DGGE is attractive technique, as it sepayate same length dsDNA according to sequence variation typical 16S rDNA genes. The diversity and similarity of bacterial community in cave was analyzed by GC341f and PRUN518r primer sets foy amplification of V3 region of eubacteria 16S rDNA. The specific DGGE band profile of the cave water gives the possibility that the specific bacterial cell can be adapting to the specific cave environment and living in the cave. The DGGE band profiles of all samples with guano were compared and analyzed by image analyzer, in which mutual band profile was compared to be and the band intensity of guano was the highest. From these result, it is thought that the guano was main nutrient source and influenced on the community structure of the cave environment where is nutritionally limited. Pseudomonas sp. NZ060, Pseudomonas pseudoalcaligenes, uncultured Variovorax sp. and soli bacterium NS7 were identified to be on some sample from analysing DNA sequence of some DGGE band.

• Proceedings of the Zoological Society Korea Conference
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• 2001.10a
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• pp.138.4-139
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• 2001

• Korean Journal of Microbiology
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• v.49 no.2
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• pp.184-190
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• 2013

Cave environment provides special ecosystems for evolution of lives distant from surface environments. We investigated bacterial and archaeal communities of wall biofilm obtained from of a volcanic cave (Daesubee) in Jeju, Republic of Korea. Bacterial and archaeal 16S rRNA genes were PCR-amplified and sequenced using pyrosequencing technologies. Unique prokaryotic communities with low diversities were observed. The main bacterial sequences (ca. 83% of total reads) were affiliated with Pseudonocardia mongoliensis of phylum Actinobacteria and clustered with clones obtained from various caves. Reflection of light on the wall surface of cave might be caused by formation of beads of water caused by hydrophobic filaments of actinobacterial colonies. Main archaeal sequences (ca. 65.7% of total reads) were related with those of I.1a-Associated group of phylum Thaumarchaeota. The sequences were related with that of Candidatus Nitrosotalea devanaterra which was known to oxidize ammonia under acidic condition (ca. pH 5.0). Nutrients leached through volcanic soils contribute formation of unique microbial communities of wall biofilm of cave Daesubee.

• Journal of the Speleological Society of Korea
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• v.34 no.35
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• pp.43-51
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• 1993

Gosu Karst Cave 지역은 우리나라에서도 Karst rocks가 가장 잘 발달된 곳이며 특히 이 지역은 고생대의 조선계 대석회암통에 해당된다. Karst Cave와 여러가지 형태의 생성물은 지질시대부터 생성에 알맞는 고기후학적인 조건과 지구화학적인 여건이 결합하여 풍화작용의 결과로 이루어진 것이다. Cave의 발달 방향은 지층의 주향과 거의 일치하며 지금도 이러한 형성작용은 진행되고 있다. Cave 내부의 기후환경의 영향으로 Cave내에 서식하는 생물은 7목 9종이 확인 및 채집되었다.

• Proceedings of the Speleological Society Conference
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• 1993.07a
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• pp.129-145
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• 1993

상근에 있는 자연과학관으로 3층건물이다. 1층은 관광코너로 자연의 아름다움과 이 풍부한 상근의 관광자원을 슬라이드 사진등으로 전시하고 있으며 하이킹코오스 기념사진관코너등을 마련하고 있다. 1층의 관광코너는 전시장에 있는데 하쯔네 화산의 함원과 지질, 암석, 온천등의 전시와 지질관측의 실제상황을 설명하고 있다. 이층 전시장에는 상근의 지형과 모든 지리적인 분포등을 알 수 있는 종합모형, 기상관측과 지구의 역사의 모습 등이 전시되고 있다. 한편 3층에는 생물사회 즉, 삼림과 습지. 계곡 그 부근에 있는 소, 호의 모습과 이들의 고장에서 서식하는 동식물의 모습을 입체적으로 전시하고 있는데 물론 박제품들로 전시되고 있다. 이 자연과학관은 최근에 설립된 것으로 부토산 소, 호를 한눈으로 전망할 수 있는 곳에 세워진 수학여행, 자연관찰, 임간학교등의 관찰코오스로서 가장 적당하다.(중략)

• Journal of Bio-Environment Control
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• v.9 no.4
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• pp.230-236
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• 2000

For extension of storability, onion bulbs produced in Cheju Island were exposed to ${\gamma}$-ray irradiation at a dosage of 0, 3, or 6Krad and stored in a natural cavern, or in low (2$^{\circ}C$) or ambient temperature storage condition. Sprouting began from approximately five months in storage, regardless of storage condition, and increased as the storage period increased. From early August, sprouting as high as 50% occurred in the natural storage cavern, and 20% in ambient temperature storage when bulbs were treated with 0 krad ${\gamma}$-ray. However, bulbs treated with 3 or 6krad ${\gamma}$-ray did not any sprouting until six months in storage. No significant weight loss was observed in all treatments until sprouting occurred from 40 days after the initiation of storage. Weight loss, however, increased remarkably after 40 days in storage, indicating that it was closely related to sprouting during storage. Bulbs stored at 2$^{\circ}C$ showed 5~10% rot, but were still edible. Percent rot reached to as high as 50% in bulbs unirradiated and stored at ambient temperatures after seven months, while it in irradiated bulbs reached to 5, 33, and 30% at 2$^{\circ}C$, in a natural cavern, and ambient temperatures, respectively. Sugar contents slightly increased in irradiated bulbs in the later storage period under ambient temperatures, while sugar contents in irradiated bulbs in the later storage period under ambient temperatures, while sugar contents in Allium vegetables generally decrease in later storage if unirradiated. Sulfur content slightly increased in irradiated bulbs in the early storage period and decreased gradually in all treatments in the later storage stage.

• Journal of the Speleological Society of Korea
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• no.89
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• pp.1-15
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• 2008

In this study, the inhabitant were investigated at the six sites in the cave which is located in Giwha-ri, Mitan-myen, Pyeong Chang-gun from January to June in 2005. As the result of observation, collection, researching, It was confirmed as all animals species were classified by 19 species, 4 classes, 11 orders, 16 families. Among the investigated animals, Diestrammena asynamora Adelung distributed 66.6% and Antrokoreana gracilipes Verhoeff distributed 22.4%. They were confirmed as a dominant species. Among the observed animals, Troglobite with ecological characteristics was 5.3%, troglophiles were 21.1% and trogloxenes were 73.7%. Among them, There were the largest number of species in trogloxenes. In the class manmalia, Rhinolphus ferrumequinum korai Kuroda and two Myolis daubentoni ussuriensis Ognev's species were observed. Among them Rhinolphus ferrumequinum korai Kuroda distributed 58.9%, so they were confirmed as a dominant species. The largest number of Triphosa dubitata Linnaeus, trogloxenes, were observed in January and February in winter. 503 individual of Antrokoreana gracilipes Verhoeffs, troglobites, were observed only in June. Diestrammena asynamora Adelung, troglophiles, appeared the large number of species in sping, especially, in April and May.

• Korean Journal of Environmental Biology
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• v.18 no.1
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• pp.53-61
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• 2000

In order to characterize the genetic diversity of bacterial community in groundwater, samples were collected from used for drinking water and polluted with heavy metal wastewater in Seoul city and natural cave of Kangwondo. The DNA was amplified with 165 rDNA-based primers by use of the PCR, and then analysed ARDRA (amplified ribosomal DNA restriction analysis). Restriction endonuclease analysis patterns of amplified 165 rDNA in drinking water and wastewater relatively showed high genetic diversity in situ and drinking groundwater. The number of DNA fragments varied with in situ and drinking water. This method of ARDRA of bacterial communities in groundwater could be used for a quick assessment of genotypic changes between different locations reflecting different environmental conditions and the diversity reflected pollution of groundwater (natural cave water>drinking water>waste water, as in order of grade). [Genetic diversity, Groundwater, 165 rDNA, PCR, ARDRA].