• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.6
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• pp.930-935
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• 2004

This study was carried out to determine the antimutagenic effect of soybean sprouts cultured in water containing germanium by Ames test and SOS chromotest. Germanium significantly inhibited the mutagenicity induced by aflatoxin B$_1$ (AFB$_1$) in Salmonella typhimurium TA100 by Ames test, and 4-nitroquinoline-N-oxide (4-NQO) in SOS chromotest. Juice from germanium treated soybean sprouts (GTS) inhibited 57∼75% mutagenicity induced by AFB$_1$, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and 4-NQO compared with 20∼48% inhibition rate of control soybean sprouts (germanium non-treated soybean sprouts, GNTS) in the Ames test. Also, methanol extracts from GTS inhibited 65% mutagenicity induced by AFB$_1$ in Salmonella typhimurium TA100, and 51% mutagenicity by 4-NQO in SOS chromotest. Therefore, it suggests that GTS has strong potential antimutagenic effect.

• Korean Journal of Environmental Biology
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• v.17 no.3
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• pp.331-335
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• 1999

The present study was carried out to investigate the combined effect of radiation and photoperiod (PP) regimes on Tradescantia 4430 somatic cell mutations. Potted plants were irradiated with 0.3, 0.5 and 1.0 Gy of gamma radiation from 60Co source. The plants irradiated only with gamma radiation were used as control group (CT). The somatic cell mutation rate in 0.5 Gy irradiated CT and PP20 group started to increase on the 6th day and reached a maximum value on the l0th day and 9th day after irradiation while the rate in the experimental group under 4 hours of photoperiod a day (PP4) started to increase on the l0th day and reached a maximal value on the 16th day post-irradiation. The slope of dose-response curve in CT was 5.99 ($r^2$=0.99), while it was 6.93 ($r^2$=0.98) in PP20 and 11.74 ($r^2$=0.99) in PP4, respectively. The biological efficacy of radiation in the induction of pink mutation increased by 15.7% in PP20 and 95.9 % in PP4, respectively. It is suggested that photoperiod regimes unfavorable to the plant have an additive effect on radiation-induced mutations and a delaying or inhibiting effect on cell damage repair, as well.

• Journal of Life Science
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• v.17 no.5 s.85
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• pp.641-645
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• 2007

We conducted a seed germination screening under saline conditions to identify salt tolerance(sto) mutants with ethyl methane sulfonate(EMS) mutagenesis seed pool. During the screening, we identified three mutant lines that seemed to confer elevated salt tolerance in high concentrations of NaCl. At 175 mM NaCl, germination rate of sto42-14 mutant(one of the EMS salt tolerance mutants) was 7-fold higher than that of wild-type plants. Interestingly, sto42-14 mutant exhibited insensitivity to high glucose concentration and growth inhibition to gibberellin. Our results suggest that sto42-14 is involved in salt stress tolerance as well as in glucose and gibberellin response in Arabidopsis.

• The Journal of Natural Sciences
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• v.9 no.1
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• pp.45-52
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• 1997

We have examined the effects of low concentrations of MNNG, alkylating agent, in survival and mutagenesis in Aspergillus nidulans. Pretreatments of cells with nontoxic and submutagenic doses of MNNG did not reduce the cytotoxic and mutagenic effects of exposure to a high concentration of drug. The results imply that adaptive responses on survival and mutagenesis for MNNG treatments do not occur in Aspergillus nidulans. In the first mitotic cell cycle during germination, the sensitivity to MNNG has been investigated at hourly time interval, and compared with that for UV irradiation. In both UV and MNNG treatments, the sensitivity increased till S cell stage, and decreased while DNA replication continued. Different from that show for UV irradiation, lethality to MNNG reached to the maximum at G2 cell stage.

• Korean Journal of Microbiology
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• v.28 no.3
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• pp.192-198
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• 1990

To determine whether the muc region of pKM101 and its mutant pSL4 is sufficient for the expression of these phenotypes, muc regions of pKM101 and pSL4 were subcloned onto the highcopy number vector pKB354 and were selected the cloned pJB200 and pJB210. The recombinant plasmids pJB200 and pJB210 were introduced into umu $C36^{-}$ uvr $A6^{-}$ (TK610) strain and determined the protection effect and mutagenecity for UV and MMS. The protection effect and mutagenecity of umu $C36^{-}$ uvr $A6^{-}$ (TK610) were supressed by muc gene of the recombinant plasmids. The muc gene of pSL4 has higher effect than that of pKM101. The recombiant plasmid pJB210(inclued muc gene of pKM101) did not affect uv-mutagenesis in the $recA^{-}$ (JC2926) mutant.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.4
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• pp.451-459
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• 2005

The protective effects of ethanol extracts from 5 seaweeds on the mutagenic and cytotoxic damage were evaluated. They were separately extracted using ethanol from dried samples at room temperature, and freeze-dried. The inhibition effects on the mutagenicity in Salmonella assay by Ames test and cancer cell inhibitory effect in HeLa cell, MCF-7 cell and SNU -638 cell by MTT assay were assayed. Seaweed fusiforme, sea tangle and green laver showed strong inhibitory effect against 2-nitrofluorene, sodium azide- or 2-anthramine-induced mutagenicities in Salmonella Typhimurium TA 98 and TA 100 at the level of 2.5 mg ethanol extract per plate. Cancer cell inhibitory effect was shown with all of the seaweed extracts. Green laver, sea mustard, sea tangle and seaweed fusiforme showed strong cytotoxicity against HeLa and MCF-7 cells, with inhibiting by $92\~93\%$ and $89\~92\%$, respectively. These data show that 5 seaweeds tested in this study might be potent functional foods for cancer prevention, and consumption of these seaweeds in adequate amount is recommended.

• Proceedings of the KIEE Conference
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• 2002.07b
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• pp.1175-1177
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• 2002

본 논문에서는 외란에 매우 강인한 특성을 갖는 유전자 알고리즘에 의한 PI 제어기를 통하여 속도 제어기를 설계하고 성능평가를 하였다. 유전자 알고리즘의 파라미터인 교배율, 돌연변이율, 세대내 개체수 세대수를 선정하여 최적해를 구하는데 적절한 선정이 필요하다. 따라서 Matlab/Simulink 환경 하에서 유도전동기 제어 시스템을 모델링하고 시뮬레이션 결과를 통해서 유전자 알고리즘의 적절한 파라미터 선정 후 제어기의 유용성을 입증하였다.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2002.12a
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• pp.375-378
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• 2002

In this paper we propose parallel GA to optimize mutation rate and crossover rate using server-client model. The performance of GA depend on the good choice of crossover and mutation rates. Although many researcher has been study about the good choice, it is still unsolved problem. proposed GA optimize crossover and mutation rates trough evolving subpopulation. In virtue of the server-client model, these parameters can be evolved rapidly with relatively low-grade

• Korean Journal of Microbiology
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• v.33 no.3
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• pp.199-202
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• 1997

The effect of electric shock (AC 38 Vll.3 cm) on the lethal effect and induction rate of mutation of chemical mutagen N-methyl-N'-nitro-N-nitrosoguanidine (NTG) was examined by using the spores of Streptomyces and the cells of haploid and polyploid yeast strains of Saccharomyccrs. Spores of Sireptomyces were all alive after 180 min of electric shock, hut all dead after 960 min-treatment. When the spores of Streptomyces or the cells of haploid and polyploid yeast were treated with electric shock and NTG, the electric shock increased the lethal effect of NTG; the survival rate of Streptomyces dropped from 72 to 48% after 180 min-treatment and those of haploidand polyploid-yeast decreased from 8 to 3% and 25 to lo%, respectively, after 40 min-treatment. The electric shock also increased mutation rates of Streptomyces and haploid yeast. from 1.8 to 13.6%' after 120 min-treatment and from 2.4 to 4.8% after 40 min-treatment, respectively.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1992.05a
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• pp.25-25
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• 1992

1.형질전환 조건 설정: 페염균을 casein hydrolysate배지에서 배양하여 550nm에서의 흡광도가 0.1이 되었을 때 pH를 7.0으로 낮추고 형질전환 능력이 나타나도록 할 때에는 pH를 7.6으로 높여 줌으로써 형질전환이 원하는 시기에 발현되도록 함. 2.돌연변이원에 대한 감수성 측정: com- mutant는 야생형보다 돌연변이가 일어난 위치에 따라 UV에 대한 감수성의 차이가 나타났으나 EMS에 대해서는 야생형과 큰 차이가 얼었음. 3.온도민감성: 5시간동안 43$^{\circ}C$에서는 37$^{\circ}C$에서의 생장율의 5%만 증가되었으나 5$0^{\circ}C$에서는 전혀 성장하지 않음.