• Title, Summary, Keyword: 돌연변이

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Antimutagenic Effects of Extracts of Curry Powder and Its Individual Spice (카레분 및 향신료 추출물의 항 돌연변이 효과)

  • 정승현;정명수;이진선;박기문
    • Food Science of Animal Resources
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    • v.22 no.4
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    • pp.352-357
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    • 2002
  • Antimutagenic effects of extracts from curry powder and its individual fourteen kinds of spices, were investigated by Ames test. The antimutagenic effects against a direct mutagen, 2-nitrofluorene(2 -NF) and two indirect mutagens, 2-anthramine(2-AT) and 2-acetamidofluorene (2-AE) in the S. typhimurium TA98 were tested. For the 2-NF, the antimutagenicity of cinnamon, fenugreek, fennel, ginger, clove, turmeric and celery seed were determined as 42, 38, 32, 28, 24, 23 and 20%, respectively. The antimutagenicity of clove against the 2-AT was the highest (116%), and followed by the order of celery seed(103%), cardamon(100%), red pepper(99%), cinnamon(92%), cumin(83%), ginger(82%), fennel(82%), coriander (71%), nutmeg(68%) and turmeric (55%). The results also showed that the antimutagenic effect of clove against the 2-AF was superior to other spices. In case of curry powder among more than 10 kinds of spices, the antimutagenenicity against the 2-AT and 2-AF showed 23% and 6%, respectively, but no effect was observed against the 2-NF.

사람 T-임파구의 HPRT유전자에서 방사선 및 환경독성물질에 노출에 의한 돌연변이 빈도의 변화

  • 윤병수;조명행;이영순;김인규;이강석
    • Proceedings of the Korean Nuclear Society Conference
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    • pp.40-45
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    • 1996
  • 사람 T-임파구에서 방사선 독성물질인 감마선과 화학적 독성물질인 PCP(pentachlorophenol)의 돌연변이 효과를 hprt (hypoxanthine phosphoribosyl transferase) 유전자의 돌연변이빈도로써 측정하였다. 감마선은 $^{137}$Cs원을 사용하여, 0 - 300 rads의 양으로 세포의 초기배양 시기에 조사하였으며, PCP는 역시 세포의 초기배양시 최종농도 0-100 ppm으로 24시간 투여 하였다. 양 돌연변이원은 hprt유전자를 돌연변이 시키어, 300rads의 조사는 대조군에 비하여 약 7.5배의 돌연변이빈도의 증가를 나타내었고, PCP 500 ppm의 처리는 대조군에 비하여 약 5배의 돌연변이빈도 증가를 나타내었다. 본 실험에 사용된 상이한 두 돌연변이원은 모두 비교적 정확한 용량-반응 관계를 보였으나, 환경독성물질들의 혼합효과에서 돌연변이원을 정성하기위하여 개선된 T-cell hprt clonal assay, 즉 reverse transcriptase/polymerase chain reaction 및 direct sequencing에 의한 mutational spectrum의 적용이 요구되었다.

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Isolation of Conditional Lethal Temperature-sensitive Mutants of Bacillus sphaericus (Bacillus sphaericus의 치사감온성 돌연변이체의 분리)

  • Kim, Young Han;Lee, Hyung Hoan
    • Microbiology and Biotechnology Letters
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    • v.13 no.1
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    • pp.41-49
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    • 1985
  • Bacillus sphaericus was mutagenized with UV light irradiation and dimethyl sulfate. Thirty-five conditional lethal temperature-sensitive(ts) mutants were isolated at the nonpermissive temperature of $42^{\circ}C$ and classified into three groups by their growth characteristics on the nutrient broth, peptone glucose yeast extract agar and mineral salts agar. First was the lethal ts group, 24 mutants, which did not grow at the nonpermissive temperature, the second, 9 mutants, was the less growth is group whose growth was restricted to one-half, and the third, 2 mutants, was the cold lethal ts group whose growth was restricted at the permissive temperature($25^{\circ}C$and $30^{\circ}C$)

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Mutational Analysis of K-ras and p53 Genes in Human Lung and Pancreatic Carcinoma Cell Lines (사람 폐암과 췌장암 세포주에서 K-ras p53 유전자의 돌연변이에 대한 연구)

  • 정경이;정노팔
    • The Korean Journal of Zoology
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    • v.39 no.3
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    • pp.231-238
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    • 1996
  • Several types of human lung and pancreatic carcinoma cell lines were cultured and their chromosomal DNAs were extracted. These DNAs were then partially amplified by PCR (Polymerase Chain Reaction) and sequenced to analyze the types and frequency of mutations, and their possible relation in the oncogene, K-ras and suppressor gene, p53. Regardless of the cell line origin, 81% were found to possess at least one mutation. Among the cell lines analyzed, 54.5% of the mutations were found in either K-ras or p53. Except for one nonsense mutation, all mutations were missense with either base insertions or substitutions. Furthermore, besides the p53 codons Known to be mutated simultaneously with' ras to enhance tumor growth, p53 164-165 and 248 were found to be mutated simultaneously with K-ms. Regardless of the site of p53 mutation, all K-ras mutations found in these cases occurred at exon 1, codon 12.

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Enhancing the Alginate Degrading Activity of Streptomyces sp. Strain M3 Alginate Lyase by Mutation (Streptomyces sp. M3 알긴산분해효소의 돌연변이에 의한 활성증대)

  • Kim, Hee-Sook
    • Journal of Life Science
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    • v.22 no.1
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    • pp.7-15
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    • 2012
  • A polyguluronate-specific lyase from Streptomyces sp. strain M3 has been previously cloned and characterized. In this study, the M3 alginate lyase gene in the pColdI vector was mutated by site-directed mutagenesis and random mutagenesis to enhance the alginate degrading activity. Six mutants were obtained: Ser25Arg, Phe99Leu, Asp142Asn, Val163Ala, Lys191Glu, and Gly194Cys. Phe99Leu and Lys191Glu mutants completely lost their alginate lyase activity, whereas the alginate degrading activity of Gly194Cys mutant increased by nearly 10 fold. The 3-D protein structure of M3 alginate lyase, which was constructed using the Swiss-Model automodeler, was also compared to the crystal structure of another alginate lyase. A mutated glycine residue was positioned between Gly193 and Tyr195 of the C-terminal conserved sequence, YFKAGXYXQ. A phenylalanine residue (at position 99) and a glycine residue (at position 194) mutated in this study were distant from the active site, but the degrading activity was strongly affected by their mutation.

Desmutagenic Action of Sugar Degradation Products (당(糖) 분해생성물의 돌연변이원성 억제작용)

  • Kim, Seon-Bong;Kim, In-Soo;Yeum, Dong-Min;Park, Yeung-Ho
    • Korean Journal of Food Science and Technology
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    • v.20 no.1
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    • pp.119-124
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    • 1988
  • The desmutagenic effects of ${\alpha}$-hydroxycarbonyl compounds, such as glyceraldehyde, glycolaldehyde, dihydroxyacetone, furfural, 5-hydroxymethylfurfural, maltol, acetol and acetoin and ${\alpha}$-dicarbonyls, such as diacetyl, glyoxal, methyl glyoxal and 2, 3-pentanedione were investigated against the mutagenic heterocyclic amines, such as Trp-P-1, Trp-P-2, Glu-P-1, Glu-P-2 and IQ. Most of the carbonyl compounds suppressed the mutagenicity of heterocyclic amines for S. typhimurium TA98, ${\alpha}$-dicarbonyl compounds showing a higher desmutagenic effect than ${\alpha}$-hydroxycarbonyl compounds. Among the ${\alpha}$-hydroxycarbonyl compounds, glyceraldehyde, glycolaldehyde and dihy-droxyacetone showed more effective desmutagenicity, and diacetyl among the ${\alpha}$-dicabonyl compounds had the highest desmutagenic effect. These carbonyl compounds alone also showed mutagenicity to S. typhimurium TA100 without S-9 mix. The reaction of carbonyl compounds with mutagenic heterocyclic amines also eliminated the mutagenicity of the former for S. typhimurium TA100.

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Screening of Mutagenic Activity of Extracts from Croaker and Pork Cooked by Various Cooking Methods (여러가지 조리방법으로 조리된 조기와 돼지고기의 돌연변이원성의 검색)

  • 이은경;이임선;신남희;정승희;구성자
    • Korean journal of food and cookery science
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    • v.11 no.1
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    • pp.77-82
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    • 1995
  • Croaker and pork were cooked by four kinds of methods(boiled, broiled, deep fried, pan fried) and their extracts were extracted with 50% methanol. The Ames test were performed on these methanol extracts, employing Salmonella typhimurium tester strain TA98 and TA100, with and without S9 mix and after nitrite treatment. The methanol extracts of cooked croaker and pork showed mutagenicity between original weight 0.0125 g/plate and 0.1 g/plate in all strains and induced a higher mutagenicity in all strains with S9 mix than without S9 mix. In all kinds of cooking methods, pork extracts showed higher mutagenicities than croaker extracts and especially the extract of pan fried croaker and pork showed high mutagenicities with S9 mix. The extract after nitrite treatment showed higher mutagenicities than that after non treatment and after treatment with nitrite, the mutagenicities of extracts were higher on TA98 than TA100.

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Effects of KCNQ1 S140G Mutation in Human Ventricular Fibrillation Mechanism

  • Jeong, Da-Un;Im, Gi-Mu
    • Proceeding of EDISON Challenge
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    • pp.665-671
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    • 2017
  • Iks 칼륨 전류에 관여하는 KCNQ1유전자의 S140G 돌연변이는 심방세동에 영향을 미치는 대표적인 돌연변이 유전자로, 심방세동과 S140G 돌연변이의 상관관계를 밝히기 위한 연구들이 많이 진행되어 왔다. 하지만 S140G 돌연변이 유전자가 심방 세동 환자의 심실 반응에 영향을 미칠 수 있다는 선행연구를 비롯하여 심방과 심실의 활동전위에 영향을 미칠 수 있는 가능성이 있음에도 불구하고, KCNQ1 S140G 돌연변이 유전자의 심실세동에 대한 영향과 그 메커니즘에 대한 연구는 부족하다. 따라서 본 연구는 KCNQ1 S140G 돌연변이 유전자가 심실세동에 미치는 영향에 대한 컴퓨터 시뮬레이션 연구를 통해 그 상관관계를 밝히고자 하였다. 이를 위해 1차원 세포 모델을 비롯하여 2차원 심실세동 반응과 3차원 전기 생리학 및 기계적 수축 시뮬레이션을 진행하였다. 3차원의 전기생리학 및 기계적 수축 시뮬레이션에서는 심실의 박출 활동을 확인하기 위한 정상 박동 시뮬레이션과 심실 세동 발생시의 심실의 변화를 확인하기 위한 세동 시뮬레이션을 각각 진행하였다. 그 결과 KCNQ1 S140G 돌연변이로 인해 심실의 Iks가 증가되었으며, 그로 인해 심실의 활동 전위기간(APD)과 불응기(ERP)가 단축되는 것을 확인할 수 있었다. 또한 활동전위 지속 곡선(APDr)과 불응기 지속 곡선(ERPr)이 완만하게 나타났으며, 심근세포의 전도파장이 감소하였다. 3차원 정상 박동 시뮬레이션의 결과 표준형에서 보다 KCNQ1 S140G 돌연변이형에서 심실이 소모하는 ATP의 양과 박출계수가 감소하였다. 3차원 세동 시뮬레이션 결과 표준형에서는 심실세동이 종결되었으나, S140G 돌연변이 형에서는 심실세동이 종결되지않고 유지되었으며, 심실세동이 빠르게 발생하였다. 결론적으로, KCNQ1 S140G 돌연변이로 인해 증가된 심실의 Iks는 심실의 박출 효율을 감소시키고 심실세동을 발생시키고 유지시키며, 부정맥 발생의 위험성을 높일 수 있다.

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Antimutagenicity of Soybean Sprouts Cultured with Germanium (게르마늄 수용액으로 재배한 콩나물의 항돌연변이 효과)

  • 김은정;이경임;박건영
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.6
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    • pp.930-935
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    • 2004
  • This study was carried out to determine the antimutagenic effect of soybean sprouts cultured in water containing germanium by Ames test and SOS chromotest. Germanium significantly inhibited the mutagenicity induced by aflatoxin B$_1$ (AFB$_1$) in Salmonella typhimurium TA100 by Ames test, and 4-nitroquinoline-N-oxide (4-NQO) in SOS chromotest. Juice from germanium treated soybean sprouts (GTS) inhibited 57∼75% mutagenicity induced by AFB$_1$, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and 4-NQO compared with 20∼48% inhibition rate of control soybean sprouts (germanium non-treated soybean sprouts, GNTS) in the Ames test. Also, methanol extracts from GTS inhibited 65% mutagenicity induced by AFB$_1$ in Salmonella typhimurium TA100, and 51% mutagenicity by 4-NQO in SOS chromotest. Therefore, it suggests that GTS has strong potential antimutagenic effect.

환경변이원 및 항변이원에 관한 연구: 살균제 Captan 및 유기인계 살충제 (Diazinon, MEP, Malathion 및 EPN)의 혼합처리가 E. coli $WP_2 S$ (uvrA-, trp-) 균주의 돌연변이와 생잔율로 미치는 영향

  • 조봉금
    • Environmental Mutagens and Carcinogens
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    • v.9 no.2
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    • pp.97-109
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    • 1989
  • 살균제 Captan, 살충제 Diazinon, MEP, Malathion 및 EPN 의 단독 처리 그리고 Captan 반복처리와 이들 살충제와 서로 다른 조합으로의 동시적, 시간적 복합처리가 E. coli WP2S(uvrA-, trp-) 균주의 돌연변이유발 빈도 및 생잔율에 미치는 영향을 검토하여 다음과 같은 결과를 얻었다. 1. Captan, Diazinon, MEP, Malathion, EPN 및 UV 의 동시적 복합처리는 이들 농약의 단독 처리에서 보다 E. coli WP2S (uvrA-, trp-)균주의 돌연변이유발빈도가 높았고 이들 농약의 단독처리효과를 합한 가산적 영향으로 나타났으며, 동일조건하에서 이들 농약의 처리농도를 배로 늘렸을 때는 배가효과를 보였으나 이 균주의 증가된 돌연변이 유발빈도는 cinnamaldehyde 30ng 및 60ng/ml 그리고 tannic acid 50ng/ml 배지로 각각 첨가했을 때 감소되었고 생잔율은 증가되었다.

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