• Korean Journal of Microbiology
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• v.46 no.1
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• pp.109-111
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• 2010

Recent studies have described various microorganisms that can degrade PAH, however, there are currently limited methods available to screen for PAH-degrading microorganisms. To screen for PAH-degrading microorganisms, a sublimation method (Alley, Jeremy F. and Lewis R. Brown. 2000. Appl. Environ. Microbiol. 66, 439-442) was modified to produce a simple screening system. In our results, there were several bacterial species capable of pyrene degradation including genera, Coryenbacterium, Gordonia, Rhodococcus, and Streptomyces, which have been screened from 350 bacterial isolates of commercial gasoline and oil-spilled sediment by the sublimation method. The main advantage of this method is that it (i) safely deposits an even, thin and visible layer of PAH onto the agar surface without the use of solvents and (ii) the quantity of PAH sublimed onto the agar can be easily controlled. Overall, this sublimation method may be an effective and simple technique to screen for PAH-degrading microorganisms.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.8
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• pp.579-585
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• 2013

Persistent Organic Pollutants (POPs) are chemical substances which persist in the environment, bioaccumulate through the food web, and pose a risk of causing adverse effects to human health and the environment. POPs are thermally stable, highly lipophilic and water-insoluble compounds which can induce various toxic influences including immunotoxicty, carcinogenicity adverse effects on reproduction, development and endocrine fuctions. Polybrominated diphenyl ethers or PBDEs, are organobromine compounds that are used as flame retardant. Because of their toxicity and persistence, the industrial production of some PBDEs is restricted under the Stockholm Convention, a treaty to control and phase out major POPs. In this study, PBDEs was measured in various site of Sihwa industrial complex to investigate concentration distribution of POPs. The levels of PBDEs in house dust samples were 0.722~44.024 ng/g-dry. In PBDEs congener, BDE-209 was relatively higher in the all sites. The concentration of BDE-209 measured over 80.0% of total PBDEs. In various site, high molecular PBDEs homologues are measured higher than low molecular.

• Journal of Food Hygiene and Safety
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• v.27 no.1
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• pp.42-49
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• 2012

This study was carried out to investigate the toxicity of food Red No.2 in the Sprague-Dawley (SD) female rat for 4 weeks. SD rats were orally administered for 28 days, with dosage of 500, 1,000, 2,000 mg/kg/day. Animals treated with food Red No.2 did not cause any death and show any clinical signs. They did not show any significant changes of body weight, feed uptake and water consumption. There were not significantly different from the control group in urinalysis, hematological, serum biochemical value and histopathological examination. In conclusion, 4 weeks of the repetitive oral medication of food Red No.2 has resulted no alteration of toxicity according to the test materials in the group of female rats with injection of 2,000 mg/kg. Therefore, food Red No.2 was not indicated to have any toxic effect in the SD rats, when it was orally administered below the dosage 2,000 mg/kg/day for 4 weeks.

• The Journal of the Convergence on Culture Technology
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• v.4 no.2
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• pp.161-169
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• 2018

This study aimed to evaluate seaweed polysaccharide extracts as a cosmetic material. To assess anti-microbial efficacy, Staphylococcus aureus (S. aureus) was treated with seaweed polysaccharide extracts and zones of inhibition were measured. In addition, the anti-inflammatory effect was confirmed in RAW 264.7cells, and seaweed polysaccharide extracts was applied to the dorsal skin of Sprague-Dawley (SD) rats to evaluate single-dose toxicity. As a results, seaweed polysaccharide extracts did not exhibit cytotoxicity at concentrations up to $1,000{\mu}g/mL$ in skin fibroblasts. Furthermore, when S. aureus was treated with 1% seaweed polysaccharide extracts, clear zones of $1.52{\pm}0.34cm$ formed, confirming sufficient anti-microbial activity. When RAW 264.7 cells were treated with seaweed polysaccharide extracts extract, nitric oxide (NO) production decreased in a concentration-dependent manner and the production of inflammation-related cytokines, such as interleukin 1 beta ($IL1{\beta}$), tumor necrosis factor alpha ($TNF{\alpha}$), and prostaglandin E2(PGE2), decreased. When seaweed polysaccharide extracts extract was applied at various concentrations to rats, symptoms did not change for more than 14 d, and there was no change in body or organ weights. In conclusion we found that seaweed polysaccharide extracts is not cytotoxic and has anti-microbial and anti-inflammatory effects. Therefore, it is suitable for use as a cosmetic material.

• Journal of Marine Bioscience and Biotechnology
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• v.2 no.2
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• pp.102-107
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• 2007

Environmental contamination becomes a great public concern as our society gets industrialized rapidly. The present study examine the role of chitosan in a effort to intervene the environmental pollutant-induced toxicity. PCB-induced neurotoxicity with respect to the PKC signaling was examined. Since the developing neuron is particularly sensitive to PCB-induced neurotoxicity, we isolated cerebellar granule cells derived from 7-day old SD rats and grew cells in culture for additional 7 days to mimic PND-14 conditions. PCB showed the alteration of PKC signaling pathway. The alteration was structure-dependent. Mono-ortho-substituted congeners at a high dose showed a significant increase of total PKC activity at [$^3H$]PDBu binding assay, indicating that mono-ortho-substituted congeners are more neuroactive than non-ortho-substituted congeners in neuronal cells. PKC isoforms were immunoblotted with respective monoclonal antibodies. PKC-beta II and -epsilon were activated with mono-ortho-substituted congeners exposure. The result suggests that the position with ortho has a higher potential of altering the signaling pathway. Alteration of PKC was blocked with treatment of high molecular weight of chitosan. The study demonstrated that the ortho position in PCBs are important in assessing the structure-activity relationship. The results suggest a potential use of marine bioactive materials as a means of nutritional intervention to prevent the harmful effects of pollutant-derived toxicity.

• Journal of the Korean Institute of Gas
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• v.20 no.4
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• pp.15-24
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• 2016

In the semiconductor industry, the risk of chemical accidents due to miscibility between the many types of chemicals and leakage of toxic chemicals has increased. In order to evaluate the reactivity with miscibility of chemicals, experimental method is the most reliable, but there is a time and cost limitations to be evaluated through experiment all the chemicals. In the study, the reactivity of process gases in the semiconductor industry was considered by the CRW (Chemical Reactivity Worksheets) 3.0 program developed by US NOAA (National Oceanic and Atmospheric Administration) and EPA. The reactivity informations with the miscibility of process gases for semiconductor industry provided, and also a KOSHA guide for the storage/separation of gas cylinders in dispensing cabinets in the semiconductor industry was proposed.

• Toxicological Research
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• v.18 no.1
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• pp.1-11
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• 2002

3-Monochloro-1,2-propanediol(3-MCPD) is currently being a matter of concern because of its toxicity. 3-MCPD produced during the acid hydrolysis of soybean products has been reported to be mutagenic, neurotoxic, nephrotoxic and spermatotoxic. Howerer, the carcinogenicity of 3-MCPD is a controversial issue over the past several decades. 3-MCPD characteristically showed a variety of toxicities in reproductive system such as, decrease in sperm number and sperm motility, infertility, loss of sperm function, and weight decrease in ovary. Due to the toxicity of 3-MCPD, exposure to 3-MCPD has been proposed to be reduced to as low a level as technologically feasible. 3-MCPD can be detected in soy sauce or non-soy sauce products. The legal limit for 3-MCPD this year has been suggested to be 20 ppb（$\mu\textrm{g}$/kg）in the European Community. In Korea, the permissible level of 3-MCPD is expected to be 0.3 ppm. In this study, 3-MCPD was toxicologically evaluated in terms of risk assessment in humans.

• Environmental Analysis Health and Toxicology
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• v.17 no.4
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• pp.273-284
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• 2002

At present, the health risks associated with the natural radionuclides of ground water have become a concern as potential social problems. However, there are no regulatory actions or control strategies for such risks. Therefore, we have investigated and discussed the risks and associated management strategies for radionuclides in other countries. US EPA has proposed MCL (300 pCi/L) and AMCL (4,000 pCi/L) for radon, and 30 ppb for uranium, 15 pCi/L for gross-alpha and 5 pCi/L for radium as final MCLs. Also, Canada, WHO and European countries have their inherent management levels. Finally, we suggested several criteria for setting guidelines in our countries including exposure related criteria such as geological distribution, occurrence, exposure probability distribution, exposure population and multimedia exposure assessment, acceptable risk, and cost -benefit analysis. The national-scale exposure and risk assessment, and economic analysis should be conducted for producing and aggregating the representative information on these criteria.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2003.05b
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• pp.265-266
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• 2003

유해대기오염물질 (HAPs)은 그간 비기준 오염물질로 분류되어 관심이 소홀했었지만, 독성, 발암성, 생체 축적과 대기중 지속성, 확산 등의 특성으로 인해 대기 중에 오랜 기간 체류하면서 오존 또는 광화학 산화물 등 2차 오염물질을 생성하고 악취의 원인 물질로도 작용하며 미량으로 건강과 환경에 악영향을 미칠 수 있어 그 중요성이 커지고 있다. 국내에서는 현재까지 환경기준항목을 중심으로 한 규제대상 물질에 대하여 배출원 조사와 배출계수 도출을 위한 기초 연구 등에 주안점을 두어 왔다. 그러나 실제적으로 인체에 미치는 영향을 감안한다면 유해대기오염물질(국내에서는 주로 특정대기유해물질로 규정되어 있음)에 의한 위해도는 매우 중요한 역할을 한다고 판단된다. (중략)

• Korean Journal of Veterinary Research
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• v.22 no.2
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• pp.239-246
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• 1982

Nonhuman primate에 자연 발생하는 신장질병에 대한 병리학적 연구를 위해 Macaque (200), Sanquinus mystax (15), Saimiri sciureus (80) 등 8종에서 총 504두를 일반 병리부검에 의해 조사하였다. 가장 빈발하는 신장 질병은 화농성, 간질성, 신장염이며 다음은 독성 신세뇨관염, 신사구체신염 및 신우신염 등이었다. 도표 3에서 비교된 바와 같이 nonhuman primate의 각 종류에 따라서 특징적인 신장염 소견을 보여주었다. 야외 원숭이 사육장에서 기르는 원숭이 군에서는 Aeromonas hydrophilia가 처음으로 중요한 본 질병의 원인체로 발견되었다.