• Title/Summary/Keyword: 대한생명

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Antibacterial and Intracellular Clearance Effect of Saururus chinensis Baill Water Extract against Salmonella typhimurium (삼백초 수용성 추출물의 Salmonella typhimurium 균에 대한 항균 및 숙주세포 내 사멸 효과 규명)

  • Kim, Dong-Hyeok;Lee, Jin-Ju;Lim, Jeong-Ju;Kim, Dae-Geun;Kim, Gon-sub;Lee, Hu-Jang;Min, Won-gi;Rhee, Man-Hee;Chang, Hong-Hee;Kim, Suk
    • Journal of agriculture & life science
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    • v.44 no.6
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    • pp.111-119
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    • 2010
  • Salmonella spp. are one of major pathogens for zoonosis in worldwide, and can replicate within host cells and generally cause enterocolitis and foodborne poisoning which represents a considerable public health burden. The present study was designated to investigate the safty for host cells, antibacterial effects of Saururus chinensis Baill water extract (SCWE) on pure culture and infection with Salmonella enterica serovar Typhimurium (S. typhimurium) in murine derived macrophage RAW 264.7 cells. The different treatment of SCWE concentration (1, 10 or $100{\mu}g/ml$) did not show any cytotoxic effect to RAW 264.7 cells for 24 h incubation. In determination of antibacterial activity of SCWE against S. typhimurium, bacterial viability was markedly decreased compared to SCWE-untreated control. In RAW 264.7 cells, SCWE significantly induced morphological change (p<0.05). In infection assay of S. typhimurium in RAW 264.7 cells pretreated with $100{\mu}g/ml$ of SCWE, which are non-cytotoxic concentration, bacterial uptake ability of macrophage was increased corresponding with morphological change, whereas bacterial survival rates within macrophage was markedly reduced comparing to that of SCWE-untreated control. Furthermore, nitric oxide (NO) production in SCWE-treated cells was slightly decreased until 24 h post infection. Taken together, these findings demonstrated that SCWE have the antibacterial activity for S. typhimurium and the protective effects against S. typhimurium infection through activating murine macrophage independent on NO, suggesting that SCWE were beneficial on the disease caused by intracellularly replicating pathogens as a safe alternatives of conventional chemotherapies.

미생물에 대한 Furfuryl-Alcohol 대사

  • 강순선
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1975.12a
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    • pp.183.3-183
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    • 1975
  • 1) 목적 : Furfural는 복소환식화합물중 오원환으로써 자연계에 널리 분포되고 있다. 공업적으로 중요한 Aldehyde로써 근대공업화학에 새로운 공해요인이 되고 있고 발효생산물에도 널리 존재하는 Furan 환화합물의 생물적 개열 대해선 거의 보고된 바가 없다.(중략)

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Medium Improvement for Chlrotetracycline Fermentation

  • 김성웅;유두영
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1975.12a
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    • pp.180.1-180
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    • 1975
  • Streptomyces aureofaciens를 이용하여 chlortetracycline 발효중 생산율을 높이는 방법에 대한 연구가 시행되었다. 지금가지 연구보고된 여러결과와는 달리 2가 금속 이온들은 어느 농도이상이 되면 각각 다른 수준으로 chlortetracycline 생산율을 저하시키는 영향이 있다는 관찰이 되었다.(중략)

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고추가루 미생물의 방사선 감수성

  • 최언호;김영배;이서래
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1976.10a
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    • pp.187.2-187
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    • 1976
  • 시판 고추가루에서 미생물을 분리하여 세균 5종과 곰팡이 13종을 동정하였고 코발트-60 감마선에 대한 이들 균주의 치사율 (D$_{10}$ 값)을 조사하였다. 또한 고추가루에 0-1,000 krad의 감마선을 처리하고 저장중 미생물의 증식여부를 관찰한 결과 세균의 발육감소를 가져왔다.

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