• Journal of Radiation Protection and Research
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• v.25 no.1
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• pp.45-51
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• 2000

The purpose of this experiment is to investigate the retention of the radiomercury among the organs in mice. We used the healthy ICR male mice and divided into two grouos. The experimental group was orally treated with squalene(200mg/kg of body weight) at two times a day(12 hrs interval) and radiomercury(0.005 uCi/g of body weight.) only one time. The control group was treated only with radiomercury as same amount of the experimental group. As the result, main retentive organs were kidney, liver, blood, heart and skull. In the control group, all of these organs showed high retention of the radiomercury at 6 hours, but in the experimental groups, all the organs significantly inhibit retention of the .radiomercury by squalene(p<0.05). We conclude that squalene inhibit retention of the radiomercury.

• Journal of Veterinary Clinics
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• v.23 no.3
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• pp.230-235
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• 2006

This study was performed in rats to find the minimum dose of melatonin that can effectively prevent the formation of postoperative intraperitoneal adhesions. Forty-two Sprague Dawley male rats were divided into six groups consisting of 7 rats, respectively. After celiotomy, five abrasions of $0.5{\times}1cm$ area were made on the antimesenteric serosal surface of the colon with a scalpel blade. The abdominal cavity was filled with 1 ml of solution containing 1 mg/kg(Mel 1), 3 mg/kg(Mel 3), 10 mg/kg(Mel 10), 30 mg/kg(Mel 30) and 5% ethanol solution(sham) through the catheter, using a sterile syringe before abdominal closure. Control group was given no adjuvant. The locations and values of adhesion were assessed through the second operation on the 14th day after the first operation. The adhesions were located on serosa to mesentery(54 of 210, 25.7%), serosa to serosa(44 of 210, 21%), serosa to omentum (12 of 210, 5.7%) and serosa to parietal peritoneum(0 of 210, 0%). The incidences of adhesion in Control, Sham, Mel 1, Mel 3, Mel 10 and Mel 30 were 68.6%, 91.4%, 57.1%, 60.1%, 17.1% and 20%, respectively. The values of adhesion separation in Mel 10 and Mel 30 group were lower than those in other groups. However, there was no significant(p<0.05) between Mel 10 and Mel 30 group. This study showed that 10 mg/kg of melatonin were effective in reducing the intraperitoneal adhesion.

• Korean Journal of Acupuncture
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• v.23 no.2
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• pp.167-176
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• 2006

목 적 : 본 연구는 태충(太衝) 및 양지혈(陽池穴) 에 대한 전침(電鍼)이 galactosamine 을 이용한 백서의 간독성(肝毒性)을 실험적으로 유발시킨 모델에서 예방효과(豫防效果)를 알아보고자 혈청중의 $\gamma\;-GTP$, GOT, GPT, LDH, total bilirubin, total cholesterol, triglyceride 의 변화를 관찰하였다. 방 법 : 간독성은 각 군들은 간독성을 유발하지 않고 무처치한 정상군, 간독성을 유발하고 난 후 무처치한 대조군, 각각 10 Hz, 50 Hz, 100 Hz 전침을 20 일간 10 회 시행한 후 간독성을 유발한 Pre 10, Pre 50, Pre 100군 등으로 분류하였다. 결 과 : 태충(太衝) 양지혈(陽池穴)에 대한 전침(電鍼) 치료(治療)의 예방효과(豫防效果)에서는 Pre 10 군에서는 ${\gamma}\;-GTP$, GOT, GPT, total cholesterol, triglyceride이 Pre 50군에서는 ${\gamma}\;-GTP$GOT, GPT, LDH, total bilirubin, total cholesterol이, Pre 100 군에서는 ${\gamma}\;-GTP$, GOT, LDH, total bilirubin, total cholesterol이 대조군에 비해 감소하였다. 결 론 : 위의 결과를 종합해보면 간독성에 대하여 태충(太衝) 양지혈(陽池穴)의 전침(電鍼)을 시행한 모든 군에서 간능과 지절대사에서 간손상에 대한 유의한 예방효과를 나타내었다.

• Journal of Periodontal and Implant Science
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• v.25 no.2
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• pp.372-385
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• 1995

본 연구의 목적은 비외과적 치주치료시 부가적으로 사용하기 위해 실험적으로 개발한 젤 형태의 테트라사이클린 및 테트라사이클린-구연산 혼합젤의 치근면에 대한 시간에 따른 활성도를 측정하고, 이를 용액 형태의 테트라사이클린 제제 또는 클로르헥시딘 들과 비교하는 것이다. 6명의 환자로 부터 18개의 발치된 치아를 실험대상으로 하였으며, 치아는 발치한 즉시 치석제거술과 치근활택술을 시행한 후 각 각 4개씩 4군으로 나누어 다옴과 같은 처치롤 하였다 ; 1) O.1% 클로르핵시딘 용액에 5분간 침전; 2) 50mg/ml의 테트라사이클린 용액에 5분간 침전; 3) 5% 테트라사이클린 젤에 5 분간 처리 ; 4) 테트라사이클린-구연산 혼합첼로 5 분간 처치; 5) 그리고 2개의 치아는 대조군으로서 멸균된 생리식염수에 5분간 처리하였다. 침전후 치아는 1ml의 tris-buffered saline이 담긴 용기에 옮겨 24시간 간격으로 탈착된 TBS용액올 교체하면서 실온에서 22일간 보관하였다. Porphyromonas gingivalis를 indicator organism으로 하여 microtiter assay를 이용하여 홉광도를 측정 함으로써 제거 된 용액 의 항 미생물 활성올 측정하였다. 1. 50mg/ml 의 테트라사이클린 수용액에 침전되었던 군은 생리식염수로 처리한 군에 비하여 17 일간 클로르헥시딘으로 처리한 군에 비하여는 16일간 항미생물 활성에 있어서 유의성 있는 차이를 보였다. 2. 테트라사이클린 젤과 테트라사이클린-구연산 혼합첼로 처리한 군은 대조군에 비하여 각 각 4일과 3일 까지 활성올 보였다. 3. 0.1% 클로르핵시딘 용액으로 처리한 군은 생리식염수로 처치한 군에 비하여 24시간 밖에 활성을 나타내지 못했다. 4. 전반척으로 테트라사이클린-구연산 혼합첼로 처리한 군에 비하여 테트라사이클린 첼로 처리한 군의 활성이 높았으나 유의성 있는 차이롤 보이지는 않았다.

• The Korean Journal of Pharmacology
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• v.29 no.2
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• pp.253-261
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• 1993

Possible changes in the role of insulin-sensitive cyclic nucleotide phosphodiesterase(PDE) in mediating the antilipolytic action of insulin were investigated in adipocytes from streptozotocin-induced diabetic rats. Isolated adipocytes prepared from epididymal adipose tissue were incubated, with or without insulin, at $37^{\circ}C$ for 15 min following pretreatment with various drugs or toxins, and three (plasma membranes, microsomal membranes, and cytosol) fractions prepared by differential centrifugation were then assayed for cAMP phosphodiesterase activity. The PDE activities only in the crude microsomal (P2) fractions were activated by insulin both in diabetic and control rats. The basal PDE activities in P2 fractions of adipocytes from diabetic rats were higher than those from control rats, although the maximal effects observed at 2 nM of insulin, $100\;{\mu}M$ of isoproterenol or the combination of both were not significantly different from each other. The insulin-stimulated PDE activities in P2 fractions of adipocytes from diabetic rats were not changed by PIA, a $A_{1}$ adenosine receptor agonist, whereas they were decreased to the basal PDE activities in those from control rats. In addition, the adipocytes from diabetic rats showed an increased sensitivity to pertussis toxin compared to those from controls. There were no differences between diabetic and control rats in the sensitivity of adipocytes to cholera toxin. These data indicate that the impaired signalling through inhibitory receptors such as adenosine receptors in adipocytes from streptozotocin-induced diabetes relates to the loss or the decreased function of $G_i$ proteins, and leads to the increased activity of the insulin-dependent PDE at the basal states.

• Journal of Digital Convergence
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• v.15 no.5
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• pp.391-400
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• 2017

The purpose of this study was to investigate the effects of smart phone video educational program on disease-related knowledge and anxiety in patients with coronary artery disease. A quasi-experimental study used a non-equivalent control group pre-post test design. A smart phone video educational program was developed and offered to the experimental group. Participants were drawn from a inpatient ward. Twenty eight were selected for the experimental group while 30 were assigned to a control group. The results of this study were as followed: Disease-related knowledge in the experimental group showed a significant increase after smart phone video education compared with the control group. But the score of anxiety did not show a significant difference between experimental and control group. Thus, it is necessary to develop interventions considering both educational and psychological factors at the same time for the patients with coronary artery disease.

• Journal of the Korean Society of Radiology
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• v.5 no.6
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• pp.335-341
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• 2011

This paper searched the affect of Water dropwort and Tumeric on radiation exposure. By experimenting 50 male mouse, ICR type 5 weeks old male 25~30g, 5 mouse was divided in to each group respectively. A : 3 Gy control group, B : 5 Gy control group, C : 3 Gy, FW, D : 5 Gy FW, E : 3 Gy LW, F : 5 Gy LW, G : 3 Gy FT, H : 5 Gy FT, I : 3 Gy LT, J : 5 Gy LT FW : Ate Water dropwort before the radiation exposure. FT : Ate Tumeric before the radiation exposure. LW : Ate Water dropwort after the radiation exposure. LT : Ate Tumeric after the radiation exposure. During the 7 day radiation, Control group (A, B) received physiological saline (500mg/kg/day) in oral administration, LW and LT group (E, F, I, J) respectively received Water dropwort or tumeric (500mg/kg/day) in oral administration after the radiation, FW and FT group (C, D, G, H) respectively received Water dropwort or tumeric (500mg/kg/day) in oral administration before the radiation. As a result, an average leukocyte rate on 5 mouse was 200K/${\mu}L$ on group A. 6.46K/${\mu}L$ on group C which was in normal rate range (1.8-10.7 K/${\mu}L$). An average leukocyte rate was 23.33K/${\mu}L$ on group G. which was not normal rate, but compare to 200K/${\mu}L$, it was closer to normal rate. An average lymphocyte rate was 118.87K/${\mu}L$ on Group A. 1.51K/${\mu}L$ on Group G. which was in normal lymphocyte rate range (0.9-9.3K/${\mu}L$). An average thrombocyte rate was 4000K/${\mu}L$ on Group A. 1107 K/${\mu}L$ on Group C. 2759.6 K/${\mu}L$ on Group G. which was in normal thrombocyte rate range (592-2972 K/${\mu}L$). From this result, it was found that when exposed to radiation, Water dropwort and Tumeric have defensive effect in a preventive level. Also from an animal experiment, it was found that blood corpuscle rate returns to a normal rate. Based on this experiment, diverse research on this field should be conducted.

• Korean Journal of Veterinary Research
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• v.40 no.3
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• pp.635-643
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• 2000

The aim of this study was to determine the effectiveness of carboxymethyl chitosan(CMC) solution on preventing intraperitoneal adhesions. In this study, 44 rats were divided into four groups ; an untreated control group and three experimental groups that were treated with 3 ml of 1, 2 or 3% CMC solution, respectively. The anti-mesenteric serosa of the ileum was exteriorized and then abraded in a standard manner by scraping with a scalpel blade to create homogenous petechial hemorrhagic surface over a $0.5{\times}1cm$ area. The adhesions were blindly assessed 2 weeks later by using a computerized tensiometer. The mean tensile strength(Newton) of formed adhesions was $2.48{\pm}0.88$ in control group, $1.86{\pm}0.56$ in the 1% CMC-treated group, $1.75{\pm}0.71$ in the 2% CMC-treated group and $1.55{\pm}0.60$ in the 3% CMC-treated group. The most favorable prevention against adhesion was achieved in the 3% CMC-treated group(p<0.01). We could conclude that CMC was effective on preventing the formation of postoperative small intestinal adhesions in the rat.

• Journal of Veterinary Clinics
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• v.25 no.4
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• pp.257-262
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• 2008

Renal ischemia-reperfusion injury is great clinical important because viability of the transplanted organ depends on the tolerance of the graft to ischemia-reperfusion injury, an inevitable processing during surgery. The purpose of this study was to investigate the effects of irrigation-aspiration in ischemia-reperfusion injury model induced by cross-clamping of renal vessels. Blood samples were collected from these dogs for measurement of kidney function and antioxidant enzyme activity, and RI at the intrarenal artery was measured at different time intervals. And the kidneys were taken for histopathologic evaluation at day 14. Kidney function (Cr and BUN) showed a significant increasing in untreated group compared to treated group. Resistive index of intrarenal artery was no significant difference among the groups. Activity of antioxidant enzymes in plasma was significant decrease in untreated group compare to control group while in treated group was no significant difference compared to control group. In histopathologic finding, treated group was showed less damage than that of untreated group. This result suggests that the processing of irrigation-aspiration is useful to reducing ischemia-reperfusion injury.

• Journal of Acupuncture Research
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• v.20 no.2
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• pp.112-122
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• 2003

연구 배경 및 목적 : 봉독은 항염증 등의 효과를 가지고 있다고 알려져 있지만 세포 증식과 관련된 봉독의 효과에 대한 고찰을 위해 본 연구(硏究)에서는, 봉독(蜂毒) 약침(藥鍼) 자극(刺戟)이 아세트산 유발(誘發) 통각(痛覺) 과민증(過敏症)을 가진 쥐의 치상회(齒狀回)에서의 세포(細胞) 증식(增植)과 COX2 발현(發現)에 미치는 영향(影響)에 대해 알아보고자 하였다. 실험방법 : 대조군, 아세트산 처치군, 아세트산 0.1mg/kg 봉독 처치군, 아세트산 1mg/kg 봉독처치군(n=5 in each group)의 네 군으로 나누고 해당 군에 일벌 봉독(蜂毒)(Sigma Chemical Co., St. Louis, MO, USA)을 양측 족삼리 경혈(ST36)에 주입시키고 30분 후 아세트산(100% acetic acid 1% 용액의 0.5ml)을 복강내로 주입하여 복부 긴장 회수를 세었으며, BrdU 양성, COX 2 양성 세포수를 면역 화학 조직법을 수행하여 세어 보았다. 결과 : 아세트산 처치군에서는 대조군에 비해 5 bromo 2' deoxyuridine 양성 세포의 수는 감소(減少)되며, 치상회(齒狀回)에서의 COX 2의 발현(發現)은 증강(增强)되는 것으로 보여졌다. 봉독(蜂毒) 주입(注入)은 아세트산 유발(誘發) 복부(腹部) 긴장(緊張) 횟수와 치상회(齒狀回)에서의 COX2 발현(發現)을 억제(抑制)하여, 치상회(齒狀回)에서의 세포(細胞) 증식(增植)을 증가(增加)시켰다. 결론 : 이번 결과(結果)에서 보면, 치상회(齒狀回)에서의 COX 2의 발현(發現)은 세포(細胞) 증식(增植) 억제(抑制)와 관련(關聯)되며 봉독(蜂毒)은 COX 2 발현(發現) 억제(抑制)를 통해 치상회(齒狀回)에서의 새로운 세포(細胞) 형성(形成)을 증가(增加)시킨다는 것을 알 수 있다.