• Microbiology and Biotechnology Letters
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• v.23 no.3
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• pp.282-287
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• 1995

Integrase (Int) carries out the cutting and resealing of attachment (att) site DNA via a covalent Int-DNA intermediate. A family of synthetic substrate DNAs was designed to accumulate Int-DNA intermediate. Int-DNA intermediates accumulated by half substrate was analyzed by SDS- KCI precipitation and restriction digestion. The results showed that Int-half DNA intermediate was circular and contained covalently bound Int molecule. Int-DNA intermediates were also trapped with three other kinds of synthetic substrates.

• Proceedings of the Korea Water Resources Association Conference
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• 2020.06a
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• pp.246-246
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• 2020

최근 우리나라에서 농업용수의 다원적 기능에 대한 공감대가 형성되고 작물생육에 필요한 관개용수로만 인식되던 농업용수의 개념이 농촌생활환경개선을 포함하는 다양한 지역용수로의 포괄적 개념으로 전환되고 있다. 농업용수는 식량생산 이외의 효용을 위한 다원적 기능을 가지며 농촌지역의 각종 생산활동과 생활조건의 유지개선을 위한 농업용수의 다원적 기능에 관한 관심이 증가하는 추세이다. 농업용수에서 발생하는 회귀수는 유역의 용수공급계획, 하천 유황의 예측, 관개용수 사용량 결정, 하천 건전화 방지, 수생생태계 보호 및 생물 다양성 확보 등 농업용수의 효율적 사용 및 환경생태유지를 위해 매우 중요한 역할을 하고 있다. 농업용수 회귀수량은 농업용수 중 하천으로 회귀하는 수량을 의미한다. 본류의 생태 유량 확보에 농업용수 회귀 수량이 기여하고 있으며 본류 하천의 환경 보전 기능을 하고 있다. 또한, 수생태계 보호 및 생물 다양성 확보 등 환경 생태 유지에 매우 중요한 역할을 하고 있다. 하지만 농업용수 회귀수량이 하천 수생태에 미치는 영향 분석 연구는 미흡한 실정이다. 따라서 본 연구의 목적은 대사 저수지 유역을 대상으로 농업용수 회귀 수량이 하류 하천 수생태에 미치는 영향을 정량적으로 평가하고자 한다. 본 연구에서는 회귀 수량은 관개용수량, 배수량, 침투량, 담수심 등 물수지 항목을 논물수지 모형에 적용하여 산정하였으며, 하류 하천의 생태유량 산정을 위해 대사저수지 하류에 위치한 생물측정망 자료를 통해 대표 어종을 선정하였다. PHABSIM 모형을 이용해 모의 대상 지역 특성 자료 및 인근 소하천 생물측정망 자료를 바탕으로 HSI 기반 대표 어종 서식처 환경에서 최적 생태유량을 산정하였다. 이를 통해 추정된 농업용수 회귀 수량에 따른 필요 유량이 어류 서식환경에 미치는 영향을 평가하였다. 본 연구 결과 대사저수지 하류 하천에서 농업용수 회귀수량이 차지하고 있는 기여도 큰 것으로 분석되었으며, 최적 생태유량과 비교한 결과 농업용수 회귀수량이 수생태(어류)에 미치는 영향이 큰 것으로 나타났다. 따라서 농업용수 회귀수량은 하류 하천의 하천유지수량뿐만 아니라 하천 환경생태유지에도 매우 중요한 역할을 하고 있음을 알 수 있다.

• Microbiology and Biotechnology Letters
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• v.26 no.6
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• pp.558-561
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• 1998

In the screening for lipid peroxidation inhibitors from edible mushroom, Agrocybe cylindracea, a bioactive compound AG 8 was isolated. The AG 8 was purified from methanol extract of its fruit body by Diaion HP-20 column chromatography, ethyl acetate extraction, and silica gel column chromatography, consecutively. Based on various NMR studies including $^1$H irradiation and HMBC experiments, the AG 8 was identified as MTA, 5'-deoxy-5'-methylthioadenosine. This compound inhibited lipid peroxidation with an $IC_{50}$/ value of 3.2 $\mu\textrm{g}$/$m\ell$. The MTA was isolated for the first time from basidiomycetes.

• Microbiology and Biotechnology Letters
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• v.6 no.4
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• pp.187-196
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• 1978

Antimetabolite N-2292 substance, an antagonist of L-aspartic acid and L-glutamic acid was isolated from the fermentation broth of Streptomyces. Taxonomical study on the producing strain made it a related species of Streptomyces albulus judged by cultural characteristics and carbon utilization. N-2292 substance was isolated as amorphous white powder with melting point at 185$^{\circ}C$. From the physicochemical characteristics of the substance, it was peptide like substance. It was active against Gram positive and Gram negative bacteria but negative against yeast and mold in its biological properties. It was reversed by L-Asp and L-Glu on the synthetic medium.

• Microbiology and Biotechnology Letters
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• v.29 no.3
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• pp.149-154
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• 2001

We have investigated the secondary metabolites from the mushroom Suillus granulatus. The methanolic extract of fruit body was separated by silica gel and Sephadex LH-20 column chromatographies. TLC and HPLC were also used for the further purification on compounds from the extracts, Nine compounds were finally isolated and their structures were assigned as 4-hydroxyphenylacetic acid 4-hydroxybenzaldehyde 2,5-dihydroxybenzoic acid methyl ester 5'-deoxy-5'methylthioadenosine. indole-3- carboxlic acid methyl ester indole 3-carboxaldehyde 1,3,5-trihydroxy 7-methylanthraquinone nicotinamide and 3-geranylgeranyl-4-hydroxybenzoic acid on the basis of NMR studies.

• Microbiology and Biotechnology Letters
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• v.18 no.1
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• pp.103-108
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• 1990

The enzymatic studies on the methylglyoxal metabolism in yeast and bacterial cells indicated that organisms are equipped with the common and manifold systems for the detoxification of methylglyoxal. Among these systems, the glyoxalase I is the most important route for methylglyoxal detoxification. The molecular structure of glyoxalase I is apparently distinct from the enzyme sources, and zinc ion is an essential cofactor in enzyme activity. The gene for Pseudomonas putida glyoxalase I functioned as a scavenger of methylglyoxal and regulated the cell size of the bacterium. Comparison of the nucleotide sequence of the P. putida glyoxalase I gene with the N-terminal amino acid sequence of the purified enzyme revealed that the N-terminal methionine residue was removed after translation. Possible physiological role of glyoxalase I was also discussed.

• Microbiology and Biotechnology Letters
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• v.34 no.3
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• pp.216-220
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• 2006

Thermophillic fungus Thermoascus aurantiacus showed excellent growth and produced high amount of alcohol oxidase and catalase in a pectin medium. Besides, the strain produced enzymes which related with pectin or alcohol decomposition. We detected extracellular pectin esterase (EC 3.1.1.11) activity and, both intracellular and extracellular pectinase (EC 4.2.2.10) activity, as pectinolytic enzymes produced by T. aurantiacus. The production of methanol decomposition enzymes, such as alcohol oxidase (AOD, EC 1.1.3.13), alcohol dehydrogenase (ADH, EC 1.1.1.1), formaldehyde dehydrogenase (FADH, EC 1.2.1.1) and formate dehydrogenase (FDH, EC 1.2.1.2) follows by pectin esterase reaction which is converted to methanol. We concluded that T. aurantiacus has pectinolytic and alcohol - oxidative enzymological mechanism which produced carbon dioxide as a final material, started from pectin.

• Food Science and Industry
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• v.50 no.1
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• pp.26-35
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• 2017

Obesity is a worldwide problem that is associated with metabolic disorders. Obesity is caused by the accumulation of an abnormal amount of body fat in adipose tissue. Adipose tissue is a major metabolic organ, and it has been classified as either white adipose tissue (WAT) or brown adipose tissue (BAT). WAT and BAT are characterized by different anatomical locations, morphological structures, functions, and gene expression patterns. WAT is mainly involved in the storage and mobilization of energy in the form of triglycerides. On the other hand, BAT specializes in dissipating energy as heat through uncoupling protein-1 (UCP-1)-mediated non-shivering thermogenesis. Novel type of brown-like adipocyte within WAT called beige/brite cells was recently discovered, and this transdifferentiation process is referred to as the "browning" or "britening" of WAT. Recently, Brown fat and/or browning of WAT have been highlights as a new therapeutic target for treatment of obesity and its related metabolic disorders. Here, we describe recent advances in the study of BAT and browning of WAT, focusing on proteomic approaches.

• KSBB Journal
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• v.25 no.4
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• pp.311-318
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• 2010

Lactic acid bacteria display a relatively simple metabolism wherein the sugar is converted mainly to lactic acid. The extensive knowledge of metabolic pathways and the increasing information of the genes involved allows for the rerouting of natural metabolic pathways by genetic and physiological engineering. In this contribution, the lactic acid bacteria as an efficient cell factory for different (food) ingredients will be presented. The emphasis will be on some successful examples of metabolic engineering and on the physiology of these bacteria, which makes them so suitable as a cell factory.

• KSBB Journal
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• v.32 no.3
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• pp.169-173
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• 2017

Butyric acid (C4 carboxylic acid) is used as an important compound in food, pharmaceutical, and chemical industries. Currently, butyric acid is mainly produced at the industrial scale through the petrochemical processes. Bio-based butyric acid has also gained attention, because the consumer prefers the food and pharmaceutical ingredients that are produced through fermentation. Clostridia is one of the well-known butyric acid producers, and massively engineered for enhanced production of butyric acid. In this paper, we reviewed the metabolic pathway of clostridia, especially Clostridium acetobutylicum and Clostridium tyrobutyricum, and summarized the metabolic engineering strategies of the strains for enhanced production of butyric acid.