• 동국한의학연구소논문집
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• 제9권
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• pp.35-49
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• 2000

본 실험은 대방풍탕(大防風湯)이 혈관생성과 세포유착인자발현 억제를 통해 LPS(lipopolysaccharide)유발 관절염 완화에 미치는 영향을 조사하기 위해 LPS주사로 관절염이 유발된 생쥐에게 대방풍탕(大防風湯) 추출액을 음용 투여하였다. 실험동물은 20g된 female Balb/c계 생쥐암컷을 선별하여 아무런 처치를 하지 않은 대조군, 무릎관절에 LPS를 주사한 LPS군, LPS주사후 대방풍탕(大防風湯) 추출액을 투여한 DBP군으로 나누어, 다시 LPS와 DBP군은 LPS처리 후 시간의 경과에 따라 3, 7, 14일로 구분하였으며, 실험군 각각에는 실험동물을 10마리씩 배정하였다. 광학 현미경(BX50)으로 대방풍탕(大防風湯) 추출액 투여가 LPS주사 후 증가된 윤활 관절막내 혈관분포 증가에 미치는 영향과 아울러 윤활세포, 섬유화 및 섬유모세포, 비만세포와 염증관여세포, 세포유착인자 등의 윤활 관절막에서의 형태변화를 관찰하였다. DBP군에서는 자유면에서 윤활세포과형성의 억제와 침윤 림프구의 감소, 낮은 섬유화 양상과 침적 섬유모세포의 감소, 모세혈관 주변의 림프구 침적 감소와 비만세포 분포감소, 자유면과 섬유지역분포ICAM과 혈관주변분포VCAM 등과 같은 세포유착인자의 감소 등이 관찰되었다. 이상의 결과로 볼 때 대방풍탕(大防風湯)은 관절염 유발시 발생되는 혈관생성과 세포유착인자의 발현증가 등을 억제함으로써 이후 발생되는 윤활 관절막 손상을 완화하는 것으로 사료된다.

• 대한한방소아과학회지
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• 제11권1호
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• pp.183-204
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• 1997

In order to study the effects of Daebangpungtang on galactosamine induced hepatoxity in rats, the experimental rats divided five groups (Normal, Control, Sample A, B, C groups). Under the same condition, normal and control groups were administered normal saline for 16 days, control group was injected to abdomen with galactosamine at 8th day (800mg/kg). Sample A group was administered the Daebangpungtang aqueous solution($200m{\ell}／kg$ p.o) for 8 days and injected galactosamine(800mg／kg i.p) for the last day and was administered normal saline for 8 days. Sample B group was treated as same as group A for 8 days, and then was administered the Daebangpungtang aqueous solution($200m{\ell}／kg$ p.o) forfurther 8 days. Sample C group was administered the Daebangpungtang aqueous solution($200m{\ell}／kg$ p.o) for 16 days. The activity of GOT, GPT, ${\gamma}$-GTP, ALP, LDH and total bilirubin in serum were measured at 8th and last day. The results were obtained as follows: 1. The activity of serum GOT of the sample A group decreased effectively at the 8th day and sample B group decreased effectively at 16th day as compared with the control group. 2. The activity of serum GPT of the sample A group decreased effectively at the 8th and 16th day and sample B group decreased effectively at 16th day as compared with the control group. 3. The activity of serum ${\gamma}$-GTP of the sample B group decreased effectively at the 16th day as compared with the control group. 4. The activity of serum ALP of the sample A group increased respectively at the 8th and 16th day and sample B group decreased effectively at 16th day as compared with the control group. 5. The activity of serum LDH of the sample A, B groups decreased effectively at 16th day as compared with the control group. 6. The activity of serum total bilirubin of the sample A, B groups decreased effectively at 16th day as compared with the control group. 7. The activity of GOT, GPT, ${\gamma}$-GTP, ALP, LDH and total bilirubin in serum of the sample C group were analogous with thats of normal group.

• Korean Journal of Acupuncture
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• 제22권3호
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• pp.83-104
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• 2005

Objectives : DaeBangPungTang(DBPT) is one of the prescriptions used for the treatment of knee arthritis in oriental medicine. The present study aimed to examine the analgesic effect of DBPT on a rat model of carrageenan-induced arthritis, and the relations between DBPT-induced analgesia and endogenous nitric oxide(NO) and inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and c-Fos protein expression in the spinal cord. Methods : Carrageenan-induced arthritis rat model was used to test the effect of DBPT as a chronic pain model. After the induction of arthritis, rats subsequently showed a reduced stepping force of the affected limb for at least tile next 4 days. The reduced stepping force of the limb was presumably due to a painful knee, since oral infection of indomethacin produced temporary improvement of weight bearing. DBPT dissolved in normal saline was minted several acupoints. After the treatment, behavioral tests measuring stepping force were periodically conducted during the next 12 hours. Results : DBPT produced significant improvement of stepping force of the hindlimb affected by the arthritis lasting at least 9 hours. The magnitude of this improvement was equivalent to that obtained after an oral injection of 3mg/kg of indomethacin and this improvement of stepping force was interpreted as an analgesic effect. DBPT produced the improvement of stepping force of the affected hindlimb in a dose-dependent manner. Both NO production and iNOS, COX-2 protein expression increased by arthritis were suppressed by DBPT. DBPT on combination with electroacupuncture (EA) produced more powerful and longer lasting improvement of stepping force of the hindlimb affected by the arthritis than either DBPT or EA did. Conclusion : The present study suggest that DBPT produces a potent analgesic effect on the chronic hee arthritis pain model in the rat and that DBPT-induced analgesia modulate endogenous NO through the suppression of iNOS/COX-2 protein expression.

• 동의생리병리학회지
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• 제16권1호
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• pp.124-132
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• 2002

Daebangpung-tang(DBPT) is one of the prescriptions used for the treatment of rheumatoid arthritis(RA) in oriental medicine. The present study aimed to examine the analgesic effect of DBPT on a rat model of CFA-induced arthritis, which is not identical to human auto-immune arthritis although it does have many features in common with RA, and the relation between DBPT-induced analgesia and steroid hormones. CFA-induced arthritis rat model used to test the effect of DBPT was chronic pain model. After the induction of arthritis, rats subsequently showed a reduced stepping force of the affected limb for at least the next 18 days. The reduced stepping force of the limb was presumably due to a painful knee, since oral injection of indomethacin produced temporary improvement of weight bearing. DBPT dissolved in water was orally administrated. After the treatment, behavioral tests measuring stepping force were periodically conducted during the next 4 hours. DBPT produce significant improvement of stepping force of the hindlimb affected by the arthritis lasting at least 3 hours. The magnitude of this improvement was equivalent to that obtained after an oral injection of 3mg/kg of indomethacin and this improvement of stepping force was interpreted as an analgesic effect. The reduced stepping force was divided into three stages(10-30g, 30-50g, and 50-70g). All experiments was performed at 50-70g of stepping force, since both DBPT and indomethacin showed the most excellent analgesic effect at 50-70g of stepping force. DBPT produced the improvement of stepping force of the affected hindlimb in a dose-dependent manner and showed analgesic effect on neuropathic pain as well. DBPT-induced analgesic effect could not be blocked by systemic injection of steroid antagonist mifepristone. The present study suggest 1) that DBPT produces a potent analgesic effect on the chronic knee arthritis pain model in the rat and 2) that steroids system does not mediate DBPT-induced analgesia.