• Title/Summary/Keyword: 단백세균

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Detection of Conserved Genes in Proteobacteria by using a COG Algorithm (COG 알고리즘을 통한 Proteobacteria의 보존적 유전자 파악)

  • 이동근;강호영;이재화;김철민
    • KSBB Journal
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    • v.17 no.6
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    • pp.560-565
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    • 2002
  • A COG(clusters of orthologous groups of proteins) algorithm was used to detect conserved genes within Proteobacteria and to figure out their relationships. Restricting comparison to the sequences of 42 procaryotes, 33 eubacteria and 16 Proteobacteria, the number of conserved genes was increased. All analyzed procaryotes shared 75 COGs. COG0195, COG0358 and COG0528 were only represented by the 42 procaryotes. Sixtyfour COGs were added as conserved genes in 33 eubacteria. Each Proteobacteria group has a unique repertoire of COGs. Metabolic COGs were more diverse in the beta Proteobacteria group than in the other groups. These results could be used to determine the origins and the evolutionary relationships of Proteobacteria. The possibilities of detecting new biological molecules is high in phylogenetically related organisms, hence the identification of useful proteins by using this algorithm is possible.

Studies on the Plant Pathogenic Corynebacteria(III) -The amino acid composition of some plant pathogenic bacteria- (Corynebacterium 속 식물병원세균에 관한 연구(III) - 식물병원세균의 아미노산 조성)

  • Kim Jong-wan;Mukoo Hideo
    • Korean journal of applied entomology
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    • v.14 no.4 s.25
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    • pp.209-213
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    • 1975
  • This paper is a report of studies to determine the amino acid composition of Plant Pathogenic Corynebacteria and to assess whether these characteristics can be correlated with the taxonomic position of these organisms. The results indicated that plant pathogenic Corynebacteria contained on average less cystein, trypthophane, histidine, phenylalanine, isoleucin and total protein than did the other genera of bacteria. However, in general, the quantities of both cell protein and amino acids contained in bacterial cells were characteristics of the species or an individual strain of the organism and were not related to its classification.

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COG 알고리즘으로 파악한 Proteobacteria의 보존적 유전자

  • Lee, Dong-Geun;Lee, Jin-Ok;Lee, Jae-Hwa
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.715-718
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    • 2003
  • A COG (clusters of orthologous groups of proteins) algorithm, protein similarities among genomes, was used to detect conserved genes and to figure out their relationships within 42 procaryote, 33 Bacteria and 16 Proteobacteria All analyzed procaryotes shared 75 COGs. COG0195, COG0358 and COG0528 were only represented by the 42 procaryotes. Sixty-four COGs were added as conserved genes in 33 eubacteria. Each Proteobacteria group has a unique repertoire of COGs. Metabolic COGs were more diverse in the beta-Proteobacteria group than in the other groups. The possibilities of detecting new biological molecules is high in phylogenetically related organisms, hence the identification of useful proteins by using this algorithm is possible.

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Predictors of Meningitis in Febrile Infants Aged 3 Months or Younger (열이 있는 3개월 이하의 영아에서 수막염의 예측에 대한 연구)

  • Song, Hyang Soon;Kim, Eun Ok;Jang, Young Taek
    • Pediatric Infection and Vaccine
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    • v.16 no.1
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    • pp.40-46
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    • 2009
  • Purpose : The purpose of this study was to identify useful predictors for diagnosing bacterial meningitis and performing CSF studies in febrile infants three months or younger. Methods : Six hundred and fifty two febrile infants with a rectal temperature ${\geq}38.0^{\circ}C$ presented from January 2003 to April 2008 and were retrospectively studied. The total white blood cell count (WBC), band count, absolute neutrophil count (ANC), quantitative C-reactive protein (CRP) and blood cultures were performed on admission. The clinical variables associated with bacterial meningitis were analyzed. Results : In patients with bacterial meningitis, the clinical variables including CRP (P=0.036), band count (P=0.037), ANC (P=0.036) and age (P=0.001) were significantly different. The area under the receiver-operating characteristic curve was 0.969 for CRP, 0.946 for the band count, 0.765 for the ANC and 0.235 for age. A CRP cutoff point of 8 mg/dL was determined to maximize both the sensitivity and specificity (sensitivity 83%, specificity 95%, likelihood ratio 16.6). A CRP concentration of <7 mg/dL "ruled-out" bacterial meningitis, with a likelihood ratio of 0.17, a posttest probability of <0.1% and negative predictive value 91%. A CRP concentration greater than 9 mg/dL had a much higher likelihood ratio (20.1) than the band count (16.6) and ANC (2.2). Conclusion : The CRP concentration was a useful laboratory test for the differential diagnosis of bacterial meningitis among febrile infants three months of age or younger. A CRP concentration of <7 mg/dL effectively ruled out bacterial meningitis; a value ${\geq}9mg/dL$ increased the clinical suspicion of bacterial meningitis and the need for CSF evaluation.

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폐섬유자원의 발효공학적 이용에 관한 연구 제4보 섬유소자화세균의 분리 및 동정

  • 성낙계;심기환
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1976.10a
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    • pp.186.2-186
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    • 1976
  • 폐섬유자원을 기질로 하여 섬유소 단세포 단백을 생산할 목적으로 225종의 균원시료에서 252주의 섬유소 자화세균을 분리하였으며 이들 중 섬유소 자화력이 아주 강한 균 1주를 동정하고 그 성상을 조사한 결과는 다음과 같다.(중략)

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A Technique to Enhance Insecticidal Efficacy Using Bt Cry Toxin Mixture and Eicosanoid Biosynthesis Inhibitor (혼합 비티 독소단백질과 아이코사노이드 생합성 억제자를 이용한 약효 증진 기술)

  • Eom, Seonghyeon;Park, Youngjin;Kim, Yonggyun
    • The Korean Journal of Pesticide Science
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    • v.19 no.3
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    • pp.301-311
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    • 2015
  • To enhance Bacillus thuringiensis (Bt) efficacy, four Cry toxins were purified from four different Bt strains and assessed in their combined efficacy. The Cry mixtures significantly expanded their target insect spectra. Bacterial culture broth of Xenorhabdus nematophila (Xn) significantly suppressed insect cellular immune response and increased Cry toxicity. The addition of Xn culture broth to Cry mixture significantly enhanced Bt efficacy in target insect spectrum and insecticidal activity.

농산폐자원의 미생물학적 이용에 관한 연구 (제4보) 직유소 단세포단백의 생산

  • Bae, Moo;Kim, Byung-Hong
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1974.06a
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    • pp.124.3-124
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    • 1974
  • 규정농도의 $NH_4OH로$ 처리한 볏짚을 기질로 분리ㆍ동경 한 섬유소 자화세균 Cellulomonas flavige na KIST 321 및 C. aurogena nov species KIST 11을 배양하여 구체단백을 생산할 때 최적 조건을 살펴 본 결과 다음과 같다.(중략)

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농산폐자원의 미생물학적 이용에 관한 연구 (제7보) 섬유소 단세포 단백 생산에 있언서 기질의 특이성에 대하여

  • Ko, Young-Hee;Lee, Gye-Jun;Bae, Moo
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1976.04a
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    • pp.183.1-183
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    • 1976
  • 우리나라에서 쉽게 얻을 수 있는 각종 섬유소 폐기물을 유일한 탄소원으로 하여 전보에서 분리 동정한 섬유소 자화 세균 Cellulomonas flavigena KIST 321을 배양하여 균단백의 생산에 대하여 살펴본 결과는 다음과 같다. 1) 당류를 탄소원으로 했을 때 cellulose꼭 xylose에서 균체생성량이 제일 많았다.(중략)

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총산폐자원의 징생물학적 이용에 관한 연구 (제3보) 섬유소자화 세균의 기질로서 볏짚의 전처리 효과

  • Bae, Moo;Kim, Byung-Hong
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1974.06a
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    • pp.124.2-124
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    • 1974
  • 전보에서 분리ㆍ등정한 섬유소 분해력이 강한 세균을 이용하여 볏짚에서 단세포단백을 생산할 목적으로 각종 알카리에 의한 볏짚의 전처리 효과를 살펴보았다. 즉, 규정농도의 알카리 용액에 24시간 실온에서 침지하여 중성이 될때까지 수세하고 건조시켜 무기염 용액에 4%첨가하여 5일간 진탕 배양한 후 균체량 증가 및 기질의 감소를 측정하여 다음과 같은 결과를 얻었다.(중략)

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