• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.21-21
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• 2001

일반적으로 세포는 방사능이나 항암제 등의 자극에 의해 DNA가 손상받았을 때 DNA를 합성하기 전, DNA변이를 복구하기 위해 cell cycle을 정지시키게 된다. pRB(retinoblatoma protein)는 이러한 cell cycle의 조절기작에서 중요한 역할을 담당하는 것으로 알려져 있다. G1기에서 S기로 진행하는 것을 조절하는 단백질인 pRB 은 E2F(cell cycle transcription factor)와 상호작용하여 cell cycle 진행에 필요한 전사활성을 억제, PCNA (proliferating cell nuclear antigen)의 합성을 저해한다. 또한, E2F와 결합된 pRB는 apoptosis를 제어하는 유전자를 조절하는 것으로 알려져 있다. Cell cycle에 영향을 미치는 항암제의 일종인 busulfan을 처리하면, 정소 내에 존재하는 대부분의 생식세포들은 사멸되고 spermatogonia만 남는 것으로 알려져 있다. 그러나 그 기작에 대해서는 자세히 연구된 바가 없다. 본 연구에서는 busulfan처리시 spermatogonial stem cell이 어떤 기작에 의해 손상받지 않고 유지되는지를 알아보고자 실험을 수행하였다. Busulfan을 처리한 마우스 (항암제 투여 후 5주)와 정상적인 13주령의 마우스의 정소로부터 각각 세포를 분리하였다. LSC (laser scanning cytometry)를 이용하여 처리군(busulfan treated mice)과 대조군(normal mature mice)에 대해 각각 DNA함량을 비교ㆍ분석한 결과 G0/G1(2N)에 머물러 있는 세포비율이 처리군에서 현저하게 증가했다 (79.3$\pm$5.5%:8.1$\pm$1.3%). Cell cycle의 G1/S check point인 pRB와 PCNA 발현을 Western blot과 면역조직학적인 방법(immunohisto-chemistry)을 이용하여 조사하였다 PCNA는 대조군과 비교해, 처리군에서 매우 낮은 수준으로 발현되었다. 면역염색된 정소단면을 살펴보면, 대조군에서는 모든 세정관에서 PCNA를 발현하는 세포가 높은 비율로 검출되었고, 처리군에서는 소수의 세정관에서 세포들이 낮은 수준으로 검출되었다. 반면에, pRB의 경우 PCNA와는 상반된 결과를 나타내어, 대조군에서는 거의 발현이 되지 않는 반면, 처리군에서는 대부분의 세정관내, 기저막을 따라 위치한 세포들에서 발현되었다. 이상의 결과는 busulfan에 의해 pRB의 인산화가 억제, pRB 와 결합된 E2F는 전사 활성이 억제되어, PCNA 합성을 저해하는 것으로 설명되어질 수 있다. 결론적으로, 인산화가 억제된 pRB (underphosphorylated RB protein)이 quiescent spermatogonial stem cell에서만 특이하게 발현하는 단백질이며, 이러한 pRB의 발현은 apoptosis를 제어하는 역할을 담당해 busulfan처리에 의해 손상받지 않고 남아있는 것으로 시사된다.

• The Journal of the Korea Contents Association
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• v.9 no.5
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• pp.118-127
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• 2009

The current global spread of communal residential culture and the trend of continuing increase of newly built apartment environment are increasing the demand of home network products for each residential space and among households. The continuing advancement of ubiquitous and the introduction and development of home network system are promoting the demand for the product design. The residential culture change and the apartment interior design change are having mutual effects on the lifestyle of residents and the product design and demand new product design. Coordination with interior products, interior style and products, product and fashion design and reconstruction and fusion between the products and their element factors are the core element of this design study. Based on this, integrated design strategy and concept research and analysis were studied for the design process, and have been expressed in three dimension design through which the design functionality and the external aesthetical design can correspond to each other. It's because the aspect of the future development prospect can be predicted in detail by presenting practical design plan.

• Journal of the Korean Society of Propulsion Engineers
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• v.3 no.4
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• pp.58-66
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• 1999

The flow characteristics of supersonic ejectors is often subject to compressibility, unsteadiness and shock wave systems. The numerical works carried out thus far have been of one-dimensional analyses or some Computational Fluid Dynamics(CFD) which has been applied to only a very simplified configuration. For the design of effective ejector-pump systems the effects of secondary mass flow on the supersonic ejector flow should be fully understood. In the present work the supersonic ejector-pump flows with a secondary mass flow were simulated using CFD. A fully implicit finite volume scheme was applied to axisymmetric compressible Navier-Stokes equations. The standard two-equation turbulence model was employed to predict turbulent stresses. The results obtained showed that the flow characteristics of constant area mixing tube types were nearly independent of the secondary flow rate, but the flow fields of ejector system with the second-throat were strongly dependent on the secondary flow rate due to the effect of the back pressure near the primary nozzle exit.

• Tunnel and Underground Space
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• v.22 no.1
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• pp.22-31
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• 2012

In this study, scaled model tests were performed to investigate the stability of an asymmetrical twin tunnels constructed in rock mass comprising alternating layers of sandstone and shale. Each of tunnels had a differently shaped section, where the one was already constructed tunnel including lining structure but the other was planned to be under construction. Four types of test models which had respectively different pillar widths and loading conditions were experimented, where both crack initiating pressures and deformation behaviors around tunnels were investigated. The cracks of pillar mainly began to appear at the interfaces of alternating layers, following additional shear displacement between layers was confirmed as one of the most important factors of pillar failure in case of the model of pillar width 0.5D. The models with shallower pillar widths proved to be unstable because of lower crack initiating pressures and more tunnel convergences than the models with thicker pillar widths. The failure and deformation behaviors of tunnels were also dependent on the loading conditions, where the model of coefficient of lateral pressure 1.0 was more stable than the other model. Futhermore, the results of FLAC analysis were qualitatively coincident with the experimental results.

• Journal of the Korean Vacuum Society
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• v.15 no.5
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• pp.541-546
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• 2006

In this study we have investigated cathodoluminescence (CL) and structural properties of thin film $ZnGa_2O_4:Mn$ oxide phosphor by using field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM), photoluminescence (PL), and cathodoluminescence. PL emission peaked at 506 nm was observed from the $ZnGa_2O_4:Mn$ phosphor target and it was attributed to the $^4T_1-^6A_1$ transition in $Mn^{2+}$ ion. The color coordinates of the emission were x = 0.09 and y = 0.67. The $ZnGa_2O_4:Mn$ films showed the excitation spectrum peaked at 294 nm by $Mn^{2+}$ ion absorption. It was found that the higher intensity of CL emission at 505 nm appears to result from the denser and closely-packed structure in $ZnGa_2O_4:Mn$ phosphor films deposited at lower pressures. The CL intensity did not show any systematic dependence on film surface roughness.

• Journal of the Korean Geotechnical Society
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• v.30 no.4
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• pp.21-34
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• 2014

This paper studies the time-dependent behaviors of tunnel and surrounding ground due to tunnel deterioration. In the first part, the literature on deterioration characteristics of tunnels was reviewed. In the second part, a numerical analysis was performed to investigate the behavior of concrete lining on the typical section of Korean high-speed rail tunnel (weathered rock) after determination of input variables related to deterioration impact. The result shows that the settlement at the crown of tunnel and surface ground increased up to 7.0% and 30.2% of the total settlements during construction stage, respectively, and the internal convergence reduction of 9.0 mm for concrete linings was generated within 30 years after completion of tunnel construction. Also the loosening height increased up to 2.55 times of tunnel height within 50 years, which is higher than that of Terzaghi's recommendation on ultimate state. Due to this process of extending zones, it is found that additional loads were applied to concrete lining with the axial stress about 3.20~3.66 MPa, which accelerates tunnel deterioration. Finally the quantitative design approach to evaluate time-dependent behavior of lining and surrounding ground due to tunnel deterioration was proposed.

• Journal of the Korea Convergence Society
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• v.9 no.9
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• pp.89-95
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• 2018

The purpose of this study was to examine the internet addiction tendency of University students. A self-reported questionnaire was completed by 354 students in the G city from March 15 to May 22, 2017. The data were analyzed by exploratory factor analysis, Pearson's correlation analysis and multiple regression analysis using the SPSS 12.0 program. Factors affecting the internet addiction tendency were using time of internet, consciousness of information ethics(soundness to internet use), self-control(p<0.05). Accordingly, it is necessary to develop mediator program in order to preventive internet addiction to enhance of consciousness of information ethics and control using time of internet and self-control.

• Journal of Korea Water Resources Association
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• v.46 no.3
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• pp.267-276
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• 2013

An Improvement of channel structure for sound fish habitat suitability enhancement is investigated. The restoration target species of Zacco Koreanus which is represented a sound aquatic ecosystem is selected by investigating results of the fish fauna and the river environments. The changes of lower channel width for enhancing suitability indices for water velocity and depth result in habitat suitability enhancement in general by PHABSIM simulation. The habitat suitability enhancement is not guaranteed consistently by the changes of lower channel is confirmed. The optimal lower channel widths at each reach are presented by genetic algorithm of optimization which is considering the enhancement of suitability indices for water velocity and depth with given instream flows. The suggested plan of the lower channel modification will contribute to the various projects for the environmental improvement of aquatic system.

• Elastomers and Composites
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• v.47 no.4
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• pp.318-328
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• 2012

In this study, the one-component curable polyurethane resin was manufactured using blocked isocyanate and hybrid technology. To prepare the one-component curable hybrid polyurethane, silica hybrids including hydroxyl group was synthesized, and the size, shape and distribution of hybrid particle in polyurethane resin were confirmed. Then the dissociation property, mechanical property and molding property of blocked isocyanate were investigated. The dissociation property of blocked isocyanate in one-component curable polyurethane became better as the heating temperature and treatment time increased. The synthesized silica hybrid had spherical appearance and size of 23~27 nm in diameter, and it was observed that the hybrid particles were homogeneously distributed in polyurethane structure. In the case of hybrid polyurethane, the mechanical property, anti-abrasion and thermal property were higher than those of general polyurethane, and it was observed that the mechanical property was maintained when the plasticizer was introduced.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.14 no.1
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• pp.65-75
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• 2002

The changes of sea bottom configuration, which may cause the coastal disasters, have been considered as social problems. It is obvious that the beach deformation is attributable to the sediment transport associated with erosion and siltation in coastal areas such esturies, channel and harbors. The prediction method and countermeasures far them, however, are not on the level of satisfaction, which indicates that make efforts should be made on developing them. Groin was constructed at Hak-Dong gravel beach to embark ship at 1996, as a result region of right of groin, severe erosion of beach is proceeding till now 1999. In this study, based on the field measurements, involved the one-line theory model which was selected for the prediction of shoreline change to prepare coastal protection methods of Hak-Deng gravel beach. Author found that the storaged sediment estimation model by Sonu and Beek(1971) is useful model at the Hak-Dong gravel beach by the use of topographical survey data from September, 1998 to September, 1999.