• Biomedical Science Letters
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• v.6 no.1
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• pp.65-72
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• 2000

In order to improve the early diagnosis of Johne's disease in ruminants, duplex polymerase chain reaction system for the detection of the etiologic agent of M. paratuberculosis and for the differentiation of other mycobacterial animal pathogens, such as M. bovis and M. avium, was applied. Genomic DNAs were purified from peripheral blood monocytes or milk macrophages and were used as templates in the duplex PCR. Detection of Mycobacterium spp. in the specimen was carried out by PCR using primer set specific to the mycobacterial 16S rDNA. And then, mycobacterial DNA-positive specimens were further differentiated with duplex PCR system which was composed of primer sets specific to 16S rDNA of M. avium complex and Is900 gene of M. paratuberculosis. The results were re-confirmed by Southern blot hybridization with oligonucleotide specific to the internal sequence of IS900 PCR amplicons. The applicability of this duplex PCR system was evaluated with DNAs extracted from clinical specimens of peripheral blood monocytes and milk macrophages. In summary, the duplex PCR amplification system described in this experiment is promising molecular technique for the early diagnosis of Johne's disease in ruminants.

• Korean Journal of Microbiology
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• v.50 no.3
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• pp.245-248
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• 2014

Pseudomonas syringae pv. actinidiae is the causal agent of bacterial canker in kiwifruit (genus Actinidia). Multilocus sequence analysis of seven housekeeping and 11 type III effector genes differentiated the virulent P. syringae pv. actinidiae isolates worldwide into three groups designated as Psa1-Psa3. In this work, a total of 12 P. syringae pv. Actinidiae strains, including three Psa1, three Psa2, three Psa3 strains isolated from Korea and three Psa3 strains from Italy, were compared based on their phenotypic properties. Strains with different geographic origins had unique growth patterns as demonstrated by growth rate at several temperatures; all tested strains exhibited maximum growth at temperatures below $22^{\circ}C$, while the growth of Psa3 strains was completely inhibited above $30^{\circ}C$. Psa3 strains isolated from Korea had longer lag phases than the Psa3 strains from Italy. The Psa2 strains were different from Psa1 and Psa3 strains in the API 20NE test, in which the Psa2 strains could not utilize potassium gluconate, capric acid and trisodium citrate. Psa3 strains isolated from Korea could hydrolyze esculin. The API ZYM test showed that ${\beta}$-glucosidase activity was detected only from Psa3 strains. The strains belonging to the three Psa groups differed with regard to their susceptibility to ampicillin, novobiocin, and oleandomycin.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.8
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• pp.615-623
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• 2016

This study was conducted to identify differences between qualities of health related to quality of life according to the general characteristics of ankylosing spondylitis patients. Overall, 275 patients who were older than 19 years, drug-maintained for over 4 weeks from Jan 13th to Feb 12th and diagnosed as ankylosing spondylitis at C university hospital participated in this study. Collected data were analyzed through descriptive statistics, t-tests, ANOVA, Scheffe's-test and multiple regression. The results of the present study revealed that Age (p=.008), Job (p<0.001), Number of comorbidity (p<0.001), Surgery for Ankylosing Spondylitis (p=0.002), Disease duration after onset symptom (p=0.010), Disease duration after diagnosis (p=0.027), Alcohol drinking (p=0.002) and Erythrocyte sedimentation rate (p=0.049) were meaningful factors to the quality of health-related life for ankylosing spondylitis patients, and multiple regression analysis showed that level of education, income, comorbidity and alcohol consumption was a significant variable. In conclusion, the results of this study indicated that, to improve quality of life, arbitration programs for improving general characteristics and considering demographic social characteristics and clinical features need to be provided. Therefore, it is expected that the results of this study would be used as actual grounds to develop integrated arbitration programs.

• Journal of the Korean Society for Marine Environment & Energy
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• v.12 no.1
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• pp.15-22
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• 2009

Auto-navigation algorithm have been studied to avoid collision and grounding of a ship due to human error. There have been many research on collision avoidance algorithms but they have been validated little on the real coastal traffic situation. In this study, a Collision Avoidance algorithm is developed by using Fuzzy algorithm and the concept of Changeable Action Space Searching (CAS). In the first step, on a basis of collision risk calculated from fuzzy algorithm in the current time(t=to), alternative Action Space for collision avoidance is planned. In the second step, next alternative Action Space for collision avoidance in the future($t=to+{\Delta}t$) is corrected and re-planned with re-evaluated collision risk. In the third step, the safest and most effective course among Action Space is selected by using optimization method in real time. In this paper, the main features of the developed collision avoidance algorithm (CAS) are introduced. CAS is implemented in the ship-handling simulator of MOERI. The performance of CAS is tested on the situation of open sea with 3 traffic ships, whose position is assumed to be informed from AIS. Own-ship is fully autonomously navigated by autopilot including the collision avoidance algorithm, CAS. Experimental results show that own-ship can successfully avoid the collision against traffic ships and the calculated courses from CAS are reasonable.

• 한국신재생에너지학회:학술대회논문집
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• 2009.11a
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• pp.298-298
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• 2009

미국의 DOE(Department of Energy)산하 EERE(Energy Efficiecy & Renewable Energy)에서 신재생 에너지 프로그램 평가를 하고 있다. 특히 General program evaluation Guide에는 많은 평가기관에서 제안되었던 5가지 방법 중 Outcome 평가는 프로그램의 목적을 얼마나 달성했는지를 측정하는 것으로 일정한 기간이 지난 후 프로그램의 목적을 달성함으로써 얻어지는 결과를 추산함으로써 그 기간 동안 프로그램이 의도했던 목적을 얼마나 잘 수행했는지를 보여준다. 한국에서 시행되고 있는 신재생에너지 보급 프로그램의 성과를 정량화 분석을 통하여 평가할 수 있어 국내 적용성이 크다. 본 연구에서는 미국 에너지부 EERE로부터 지원받아 NREL(National Renewable Energy Laboratory)에서 실행한 Utility Green Pricing 프로그램을 사례 분석하였다. Utility Green Pricing 프로그램은 1993년 시작되었으며 2002년 말 미국 전역의 300여개 시설을 통한 90개의 프로그램을 발전시킴으로써 소비자가 자발적으로 재생에너지 발전을 지원하기 위한 한 방법으로 최근 이런 프로그램 수는 증가하고 있다. 2002년 말 기준으로 국가 전기 사용자의 20%, 대략 270,000명의 소비자가 선택하였고 2002년 290MW급 재생에너지 설비용량과 2003년 140MW급 시설을 담당하였으며 미국 이외에 캐나다, 일본, 호주, 12개 유럽 국가들이 시행하고 있다. 이 프로그램 목표는 어떤 프로그램이 green pricing 프로그램의 소비자 참가와 재생에너지 구매의 최대화를 돕는지를 제공하는 것으로 Data Pool은 90개 프로그램 중 더 이상 실행하지 않는 4개를 제외한 86개로 응답률은 77%였다. 분석대상은 거주자와 비거주자로써 자료는 2003년 초 66개의 green pricing 프로그램의 질문서를 토대로 도출되었다. Green Pricing 프로그램 질문서는 참여, 지난해 판매, 재생에너지 공급, 할 증금(초과 구매력), 프로그램 디자인과 실행, 마케팅, 소비자를 위한 부가서비스 이렇게 총 7부분으로 나누어져있다. 특히 거주자와 비거주자와 참여, 재생에너지 판매량, 프로그램 수명, 사용하는 재생에너지의 종류, 프로그램 가격, 비용 할증료, 프로그램 평가의 종류, 프로그램 마케팅 노력, 참여자에게 제공되는 부수적 가치와 관련된 자료를 모으기 위해 실행되었다. 종속적 변수로 프로그램 유효성 측정과 관련된 비/거주자 참여와 재생에너지 구매를 선택하였으며 독립적 변수로 프로그램 디자인과 마케팅 특징을 들었다. 분석방법으로는 이변성과 다변성 통계적 분석을 이용하였다. 이변성 분석은 쉽게 개별적 독립 변수와 종속변수와의 직접적 관계를 보여주며 다변성 분석은 보다 정확한 영향 산정을 위해 선택한 복합적 독립변수를 이용하였다. 프로그램 유효성 측정을 위해 비/거주자 참여 퍼센트와 재생에너지 구매 퍼센트를 구하였다. 그러나 이 분석에 몇 가지 제한점이 있었다. 소비자 중 국외자의 영향으로 분석에 많은 영향을 주지 않지만 single utility 프로그램은 비거주자참여율과 재생에너지 구매율에 영향을 끼칠 가능성이 있으며 독립/설병변수를 프로그램성공을 위해 거듭 강조하지만 빠지거나 무딘 변수는 여전히 존재한다. 분석 대상이 2002년까지를 대상으로 하기 때문에 시간상의 지체 역시 문제가 될 수 있다. 다중공산성 역시 독립변수간의 상관관계가 나타나면서 발생된다. 하지만 몇 가지 재미있는 결과를 얻어냈다. 프로그램 기간이 소비자 응답에 영향을 미치며 초기 거주고객의 높은 구매력이 지속된다는 것이다. 가격 할증료와 최소 월사용료는 프로그램 성공 초기 결정요인이 되지 않는다. 작은 시설이 성공 달성가능성이 더 큰 것으로 나타났으며 비거주자의 사적 이득 제공 역시 성공 강화에 영향을 끼쳤다.

• Korean Journal of Psychosomatic Medicine
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• v.15 no.1
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• pp.44-50
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• 2007

Objectives: previous studies have suggested the possible predisposing personality in fibromyalgia(FM). The aim of this study is to investigate the personality traits in female patients with FM compared to healthy female controls using the Temperament and Character Inventory(TCI) and furthermore, to evaluate the personality traits affecting the severity of the symptoms of FM. Methods Forty female patients with FM and 40 healthy female controls were examined using the TCI and the Hamilton depression rating scale. The FM patients were also evaluated the severity of the symptoms of FM using the fibromyalgia impact questionnaire(FIQ). Results : ANCOVA with age and depression levels as covariates showed that the FM patients scored significantly higher on the harm avoidance(F=2.187, p=0.047) and lower on the self-directedness(F=4.551, p=0.036) than the healthy controls. The FIQ scores showed positive correlation with the harm avoidance scores in the FM patients when adjusting for age and depression level(R=0.399, p=0.013) Multiple linear regression models revealed that the harm avoidance scores would affect the FIQ scores in FM patients(95% Cl=0.004-1.942, p=0.049). Conclusions : These results suggest that the female FM patients have distinctive temperament and character dimensions compared to the healthy female controls. Moreover, the harm avoidance may be the predictive personality to affect the severity of the symptoms of female FM patients.

• Journal of Internet Computing and Services
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• v.15 no.4
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• pp.125-133
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• 2014

The column-oriented database storage is a very advanced model for large-volume data analysis systems because of its superior I/O performance. Traditional data storages exploit row-oriented storage where the attributes of a record are placed contiguously in hard disk for fast write operations. However, for search-mostly datawarehouse systems, column-oriented storage has become a more proper model because of its superior read performance. Recently, solid state drive using MLC flash memory is largely recognized as the preferred storage media for high-speed data analysis systems. The features of non-volatility, low power consumption, and fast access time for read operations are sufficient grounds to support flash memory as major storage components of modern database servers. However, we need to improve traditional transaction management scheme due to the relatively slow characteristics of column compression and flash operation as compared to RAM memory. In this research, we propose a new scheme called Column-aware Multi-Version Locking (CaMVL) scheme for efficient transaction processing. CaMVL improves transaction performance by using compression lock and multi version reads for efficiently handling slow flash write/erase operation in lock management process. We also propose a simulation model to show the performance of CaMVL. Based on the results of the performance evaluation, we conclude that CaMVL scheme outperforms the traditional scheme.

• Journal of Life Science
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• v.30 no.11
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• pp.1012-1020
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• 2020

Remicade is a therapeutic biosimilar natural antibody in which the mouse variable domain has been linked to the human constant domain. It is a chimeric monoclonal antibody specific to tumor necrosis factor-alpha (TNF-α) and has been developed for the treatment of rheumatoid arthritis. To investigate the biological activity of the Remicade antibody, we carried out a bioinformatics study using a protein data bank to characterize the TNF-α antigen binding mechanism of the Remicade natural antibody. Because the production of the Remicade antibody is often limited by genetic instability of the natural antibody-producing cell, we generated a Remicade single-chain variable domain fragment antibody (Remicade) in which a heavy chain variable domain (VH) is joined with a light chain variable domain (VL) by a polypeptide linker. Furthermore, Remicade was fused to a leucine zipper (RemicadeScZip) for higher production and higher antigen-binding activity than Remicade. The Remicade and Remicade ScZip were expressed in Escherichia coli and purified by a Ni⁺-NTA-agarose column. As expected, the purified proteins had migrated as 28.80 kDa and 33.96 kDa in sodium dodecyl sulfate-polyacrylamide electrophoresis. The TNF-α antigen binding activity of Remicade was not observed by ELISA and western blot. In contrast, RemicadeScZip showed antigen-binding activity. Additional bio-layer interferometry analysis confirmed the antigen-binding activity of RemicadeScZip, suggesting that the leucine zipper stabilized the folding of RemicadeScZip in a denatured condition and improved the TNF-α antigenbinding activity.

• Journal of the Mineralogical Society of Korea
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• v.18 no.3 s.45
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• pp.205-214
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• 2005

Some Quaternary faults developed in the eastern block of the Ulsan fault are Gaegok 1, Gaegok 2, Singye, Madong, Wonwonsa and Jinhyeon faults, which are characterized by thin gouge and narrow cataclasitic tones. This study was performed to emphasize the role of mineral alteration and microtexture in response to hydrothermal alteration of fault gouges during fault activity, using XRD, EPMA, BSE (backscattered electron image), and K-Ar age dating methods. Alteration minerals in fault gouges were formed in the age range of $44.3\~28.9Ma$ by hydrothermal alteration attributed to fault activity. XRD results show that fault gouges consist predominantly of clay minerals, quartz and feldspars. Clay minerals formed in the gouge zones are mainly composed of smectite with trace chlorite, illite and kaolinite. The evidence to support the hydrothermal alteration of preexisting minerals due to fault activity are easily recognized at the host rocks in contact with gouges zones. Injected gouge and calcite veins indicate that they were originated from multiple deformation by repeated fault activity. Gouge with green or greenish grey color, for example Jinhyeon fault, contains higher $Al_2O_3$ and lower MgO and CaO compared to those with reddish color. Various colors of fault gouge are intimately related to the chemical compositions of main constituent mineral as well as mineral assemblage.

• Journal of the Korean Institute of Intelligent Systems
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• v.18 no.6
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• pp.754-761
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• 2008

As the automobile industry and technologies are developed, driver's tend to more concern about service matters than mechanical matters. For this reason, interests about recognition of human knowledge and emotion to make safe and convenient driving environment for driver are increasing more and more. recognition of human knowledge and emotion are emotion engineering technology which has been studied since the late 1980s to provide people with human-friendly services. Emotion engineering technology analyzes people's emotion through their faces, voices and gestures, so if we use this technology for automobile, we can supply drivels with various kinds of service for each driver's situation and help them drive safely. Furthermore, we can prevent accidents which are caused by careless driving or dozing off while driving by recognizing driver's gestures. the purpose of this paper is to develop a system which can recognize states of driver's emotion and attention for safe driving. First of all, we detect a signals of driver's emotion by using bio-motion signals, sleepiness and attention, and then we build several types of databases. by analyzing this databases, we find some special features about drivers' emotion, sleepiness and attention, and fuse the results through Multi-Modal method so that it is possible to develop the system.