• Journal of Radiation Protection and Research
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• v.34 no.4
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• pp.184-189
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• 2009

In pressurized heavy water reactors, workers who enter radiation controlled areas must submit their urine samples to health physicists after radiation work; these samples are then used to monitor internal radiation exposure from tritium intake. This procedure assumes that the samples submitted represent tritium concentration inside the body at equilibrium. According to both technical reports from the International Commission on Radiological Protection and experimental results from Canadian nuclear utilities, tritium inside the body generally reaches equilibrium concentration after approximately 2-3 hours of intake. In practice, urine samples can be submitted either before the 2 hours mark or after several hours of radiation work because of the numerous tasks that workers must perform and their frequent entries during nuclear power plant maintenance. In this paper, tritium concentration in workers' urine samples was measured as a function of time submitted after radiation work. Based on the measurement results, changes in the tritium concentration inside the body and its effect on internal dose assessment were then analyzed. As a result, it was found that tritium concentration reaches equilibrium concentration before the 2 hours mark for most workers' urine samples.

• Journal of Radiation Protection and Research
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• v.38 no.2
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• pp.52-59
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• 2013

The internal contamination screening method using gross beta measurement was performed for radioisotope workers. 24 h and spot urine samples from workers of medical isotope production facilities were collected and measured. Most of the results were similar with the background level of gross beta activity except for a specific worker. Gross beta activity was slightly increased in several hours after finishing work. And the environmental factor of production facilities causing internal contamination were estimated based on screening results. The additional detailed internal dose assessment must be followed after the screening for protection of workers. Moreover, a procedure was established to apply a simple internal contamination assessment for radiation workers.

• Proceedings of the Korean Nuclear Society Conference
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• 2001.05a
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• pp.401.1-401
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• 2001

• Analytical Science and Technology
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• v.20 no.3
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• pp.204-212
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• 2007

The study was carried out to investigate the ratio of ethanol to n-propanol in blood and urine specimens, and developed a method for distinguishing ingested ethanol from artifactual ethanol in urine samples. In case of no urinary ethanol was detected, the ratio of ethanol to n-propanol concentration was about 12~20 times higher than those of blood. Therefore, it might be a good method to determine whether the detected ethanol is from drinking or from microbial fermentation. During the metabolism of ethanol, the levels of the metabolite of serotonin (5-hydroxytryptamine, 5-HT) and 5-hydroxyindole-3-acetic acid (5-HIAA) were decreased, while 5-hydroxytryptophol (5-HTOL) was increased. The levels of 5-HTOL/5-HIAA in urine samples of drinking suspects were greater than 1, in that of no drinking suspects were less than 1.

• The Korean Journal of Pesticide Science
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• v.4 no.1
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• pp.1-10
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• 2000

The purpose of this study is to establish the assessment techniques of hazardous chemicals by the development of analytical method of biological samples. In this study, we have developed an extraction method of nine pesticides used for rice paddy that resulted in high recovery from the spiked human urine by the liquid-liquid extraction with diethyl ether at pH 7.0. Calibration curve obtained from each pesticide standard using by gas chromatography/mass spectrometry/selected ion monitoring has shown good linearity and detection limits were the range of $0.4{\sim}2.0$ ng/mL in urine. As a biological monitoring, urine samples of local farmers exposed directly to nine pesticides in the field were collected and analyzed by GC/MS. Of the tested pesticides, metabolites of phenthoate assumed were identified by GC/MS analysis. No parent compound was detected.

• Analytical Science and Technology
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• v.9 no.4
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• pp.336-344
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• 1996

The extraction of trace cobalt, copper, nickel, cadmium, lead and zinc in urine samples of organic and alkali metal matrix into chloroform by the complex with a dithizone was studied for graphite furnace AAS determination. Various experimental conditions such as the pretreatment of urine, the pH of sample solution, and dithizone concentration in a solvent were optimized for the effective extraction, and some essential conditions were also studied for the back-extraction and digestion as well. All organic materials in 100 mL urine were destructed by the digestion with conc. $HNO_3$ 30 mL and 30% $H_2O_2$ 50 mL. Here, $H_2O_2$ was added dropwise with each 5.0 mL, serially. Analytes were extracted into 15.0 mL chloroform of 0.1% dithizone from the digested urine at pH 8.0 by shaking for 90 minutes. The pH was adjusted with a commercial buffer solution. Among analytes, cadmium, lead and zinc were back-extracted to 10.00 mL of 0.2 M $HNO_3$ from the solvent for the determination, and after the organic solvent was evaporated, others were dissolved with $HNO_3-H_2O_2$ and diluted to 10.00 mL with a deionized water. Synthetic digested urines were used to obtain optimum conditions and to plot calibration-eurves. Average recoveries of 77 to 109% for each element were obtained in sample solutions in which given amounts of analytes were added, and detection limits were Cd 0.09, Pb 0.59, Zn 0.18, Co 0.24, Cu 1.3 and Ni 1.7 ng/mL, respectively. It was concluded that this method could be applied for the determination of heavy elements in urine samples without any interferences of organic materials and major alkaline elements.

• Proceedings of the Korean Nuclear Society Conference
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• 2002.10a
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• pp.356.2-356
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• 2002

• Analytical Science and Technology
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• v.17 no.4
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• pp.337-346
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• 2004

7-keto-dehydroepiandrosterone-acetate (7-keto-DHEA-acetate) is an anabolic steroids, and we studied basically to the metabolites of it after human dosing. We tested the matrix effect from human urine to detect the 7-keto-DHEA-acetate. And LC/ESI/MS and GC/MSD was used to detect the metabolites in dosed urine. We found the some unknown compound from dosed urine (M1, M2, M3, M4 and M5), and from these results, we supposed that these compounds have the more than 3 hydroxyl and/or ketone group. Metabolite M1 was supposed that molecular weight is 302 and 3-,17-diketone and 7-hydroxyl compound (7-OH-androstendione). Metabolite M2 was supposed that the molecular weight was same to M1 and 7-,17-diketone and 3-hydroxyl compound (7-keto-DHEA).

• Analytical Science and Technology
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• v.11 no.3
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• pp.167-173
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• 1998

An electrothermal vaporization-hollow cathode glow discharge-atomic emission spectrometer(ETV-HCGD-AES) has been developed for detecting heavy metals in human serum and urine samples. Fisrt of all, we designed a glow discharge cell for atomic emission spectrometry and its analytical performance was studied with the standard reference materials(SRMs) purchased from the NIST. Practically, the ETV-HCGD-AES demonstrated better instrumental sensitivity and selectivity for detecting Hg and Pb in the SRMs, serum and urine, than ICP-OES since the ETV-HCGD-AES was not required the complicate sample digestion procedure, which improved sample transportation efficiency.

• Analytical Science and Technology
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• v.19 no.2
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• pp.149-154
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• 2006

A gas chromatography-mass spectrometric method was developed for the determination of formaldehyde in urine and saliva. In a 20 mL glass tube, 0.2 mL of urine or saliva was taken. Further, 1.8 mL of 0.1 M HCl, 0.1 mL of 2,000 mg/L 2,4-dinitrophenyl hydrazine and $20{\mu}l$ of 500 mg/L acetone-$d_6$ as internal standard were added in the tube and sealed tightly with cap. The solution was shaken for 20 min at room temperature and extracted using 4 mL of toluene. The extract was concentrated and redissolved with $100{\mu}l$ of acetonitrile, and then measured by gas chromatography-mass spectrometer (selected ion monitoring). The detection limit was 2.0 ng/mL and 0.5 ng/mL in saliva and urine, respectively. The calibration curves showed good linearity with r = 0.997 and 0.998 for saliva and urine, respectively. The method was used to analyze formaldehyde in rat urine after oral exposure. The developed method may be use ful to the monitoring for formaldehyde exposure in human.