• Title/Summary/Keyword: 냉각 회로

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재래돼지 수정란의 동결보존에 관한 연구

  • 연성흠;허태영;강석진;서국현;최선호;이장희;박성재;류일선;김남철
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.132-132
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    • 2003
  • 멸종위험성이 높은 재래돼지를 유전자원으로서 안전하게 보존하고 유전적 다양성을 유지하기 위해 체내수정란의 동결보존 방법을 수행하였다. 재래돼지의 과배란유기는 altrenogest를 1일 20mg씩 18일 경구투여하고 PMSG 500~l,000IU 근육주사후 80시간에 hCG 500~750IU를 근육주사하였다. 발정이 관찰된 개체는 발정개시후 12시간과 24시간에 자연교배 또는 액상정액을 이용하여 2회씩 수정시켰다. 최종 수정후 5일째에 외과적으로 개복수술하여 FBS가 5% 첨가된 D-PBS 관류액으로 자궁으로부터 수정란을 회수하여 상실기, 배반포기, 확장배반포기의 수정란으로 구분하였다. 회수된 수정란은 FBS가 20% 첨가된 D-PBS의 0.4, 0.8, 1.4M glycerol 항동해제에 각 단계별로 10분씩 평형시킨후 수정란동결기(CL863, Australia)를 이용하여 18$^{\circ}C$부터 -7$^{\circ}C$까지 2$^{\circ}C$/min의 냉각속도와 -7$^{\circ}C$부터 -35$^{\circ}C$까지 0.5$^{\circ}C$/min의 동결속도(실험1), 1$^{\circ}C$/min의 냉각속도와 0.3$^{\circ}C$/min의 동결속도(실험 2)로 동결시켰다. 또한 FBS가 20% 첨가된 D-PBS의 ethylene glyco1(EG) 1.8M의 항동제에 15분간 평형시킨후 실험 1과 동일한 방법으로 동결시켰다(실험 3). 동결수정란의 융해는 37$^{\circ}C$의 항온수조에서 30초간 실시하였다. 항동해제로 glycerol을 이용한 수정란은 융해후 3가지 농도로 0.3M sucrose, 0.8M glycerol, 0.4M glycerol을 첨가한 D-PBS에 각각 10분씩 단계적으로 정치시킨 다음, 10% FBS 첨가 mNCSU-23으로 3회 세척했다. 항동해제로 EG를 이용한 수정란은 융해후 즉시 D-PBS에 각각 10분간 정치시킨 다음, 10% FBS 첨가 mNCSU-23으로 3회 세척했다. 항동해제가 제거된 수정란은 FBS가 10% 첨가된 mNCSU-23 배양액에서 39$^{\circ}C$, 5% $CO_2$ 배양기에 48시간 배양하면서 생존여부를 판단하였다. 실험 2에서 확장배반포배 수정란이 25.3%의 생존율을 나타내었으며, 실험 1과 실험 3에서는 수정란의 형태와 관계없이 생존성을 확인할 수 없었다. 이상의 결과로 보아 glycerol 완만동결에서는 확장배반포기 수정란 이상이 보존가능한 것으로 추정되나 더 추가적인 연구가 요구된다.

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The Development of Insecticidal Soaps and Organic Control of Aphid (CODEX 유기농업허용 살충비누 제조와 진딧물 방제연구)

  • Lee, Tae-Geun;Yoon, Sung-Hee;Park, Dong-Yun
    • Korean Journal of Organic Agriculture
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    • v.10 no.3
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    • pp.87-99
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    • 2002
  • The development of insecticidal soaps made by various fatty acids and organic control for insect by using of insecticidal soap(fatty acid salts) were employed. And the results were as follows: 1. To determine the input volume of potassium hydroxide for saponification of fatty acids, there were compared to individual acid value of fatty acids. in case of coconut fatty acids for saponification was 266.3 mg of potassium hydroxide(266 g/1kg of fatty acid) was calculated. 2. To make the 25% soap content by coconut fatty acids, there was required for the 266g of potassium hydroxide and 3,459 liter of soft water. Then the liquor of soap was 4,644 liter. 3. The progress of insecticidal soap made by fatty acids was accomplished indirect heating and stirring reactor (1)to make the volume of potassium hydroxide solution and warming up(90$^{\circ}$) (2)input volume of individual fatty acid (3)more than 30 min stirring reaction (4)cooling progress. 4. Insecticidal value of insecticidal soap was observed more than 92% the dilution of 50 dilution solution in consecutive 5 days of 2 treatments of the 25% soap made by coconut fatty acids on the red pepper. And insecticidal value of insecticidal soap was observed more than 94% the 100 dilution solution in consecutive 5 days of 3 treatments of the 25% soap made by coconut fatty acids on red pepper and cabbage. 5. The treatment of two times of 25% soap made by coconut fatty acids at the 50 dilution solution and 100 dilution solution in spider mite on red bean has 100% insecticidal value. 6. There was no observation phytotoxic sypmtons on red pepper in field, except for 25% soap made by carprylic acid. 7. Over the two times over 0.1% in addition of isopropyl alcohol was to improvement the insecticidal effect, but there was no effect in addition of diatomaceous earth.

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Performance Analysis of Two-Loop Rankine Cycle for Engine Waste Heat Recovery (엔진 폐열 회수를 위한 이중 회로 랭킨 사이클 성능 해석)

  • Kim, Young Min;Shin, Dong Gil;Kim, Chang Gi;Woo, Se Jong;Choi, Byung Chul
    • Journal of Energy Engineering
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    • v.21 no.4
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    • pp.402-410
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    • 2012
  • A two-loop Rankine cycle for engine waste heat recovery of gasoline vehicle has been investigated. Water-steam cycle as a high-temperature (HT) loop for exhaust gas heat recovery and R-134a cycle as a low-temperature (LT) loop for both heat recovery of the engine coolant and the residual heat from the HT loop were considered. Energy and exergy analysis was performed to investigate the performance of the system. Because two volumetric expanders are used for the HT and LT loop, the sizes of two expanders are very important for the optimization of the system. The effects of pressure ratio of the HT loop, considering the size of the HT expander, and the condensation temperature of LT loop on the performance of the system at a target engine condition were investigated. This study shows that about 20% of additional power from the engine waste heat recovery can be obtained at the target engine condition.

Development of Dust Recycling System and Dust Cleaner in Pipe during Vitrification of Simulated Non-Radioactive Waste (모의 비방사성폐기물의 유리화시 발생 분진의 재순환처리장치 및 배관 내 침적분진에 의한 막힘 방지용 제진장치의 개발)

  • Choi Jong-Seo;You Young-Hwan;Park Seung-Chul;Choi Seok-Mo;Hwang Tae-Won;Shin Sang-Woon
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2005.06a
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    • pp.110-120
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    • 2005
  • For utilizing vitrification to treat low and intermediate level waste, industrial pilot plant was designed and constructed in October 1999 at Daejon, Korea through the joint research program among NETEC, MOBIS and SGN. More than 70 tests were performed on simulated IER, DAW etc. including key nuclide surrogate(Cs, Co); this plant has been shown to vitrify the target waste effectively and safely, however, some dust are generated from the HTF(High Temperature Filter) as a secondary waste. In case of long term operation, it is also concerned that pipe plugging can be occurred due to deposited dust in cooling pipe namely, connecting pipe between CCM(Cold Crucible Melter) and HTF. In this regard, we have developed the special complementary system of the off-gas treatment system to recycle the dust from HTF to CCM and to remove the interior dust of cooling pipe. Main concept of the dust recycling is to feed the dust to the CCM as a slurry state; this system is regarded as of an important position in the viewpoint of volume reduction, waste disposal cost and glass melt control in CCM. The role of DRS(Dust Recycling System) is to recycle the major glass components and key nuclides; this system is served to lower glass viscosity and increase waste solubility by recycling B, Na, Li components into glass melt and also to re-entrain and incorporate into glass melt like Cs, Co. Therefore dust recycling is helpful to control the molten glass; it is unnecessary to consider a separate dust treatment system like a cementation equipment. The effects of Dust Cleaner are to prevent the pipe plugging due to dust and to treat the deposited dust by raking the dust into CCM. During the pilot vitrification test, overall performance assessment was successfully performed; DRS and Dust Cleaner are found to be useful and effective for recycling the dust from HTF and also removing the dust in cooling pipe. The obtained operational data and operational experiences will be used as a basis of the commercial facility.

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Cooling Effect of Air in Greenhouse Using A Fog Sprayer Consisted of Two-fluid Nozzle with Turbo Fan (터보 팬 2류체 노즐로 구성한 포그 분무장치를 이용한 온실 내 공기의 냉각 효과)

  • Kim, Tae-Kyu;Min, Young-Bong;Kim, Do-Wan;Kim, Myung-Kyu;Moon, Sung-Dong;Chung, Tae-Sang
    • Journal of agriculture & life science
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    • v.46 no.3
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    • pp.119-127
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    • 2012
  • For the promotion of the evaporative cooling efficiency of hot air in greenhouse in summer, a fog sprayer consisted of a high volume spraying two-fluid nozzle with turbo fan and a blowing fan was set up at 2.2 m height from bottom of small glass greenhouse and tested to estimate the possibility of the greenhouse cooling. The mean droplet size and the volume sprayed by one of fog sprayer were $29{\mu}m$ and $160m{\ell}/min$. All the droplets sprayed and blown by the fog sprayer were evaporated within 2 m radius. The result from the cooling test that two sprayers set up in glass greenhouse of plane area $228m^2$ was represented lower cooling effect that the temperature and relative humidity of inside air of greenhouse were $28.8^{\circ}C$ and 87.5% when those of outside air of greenhouse were $30.2^{\circ}C$ and 81.2%. Through investigation of literatures and results of the cooling test, it was estimated that the water spraying rate of evaporative cooling of single span greenhouse with 50% light curtain and with air change rate of 1 volume/min was $10m{\ell}/min/m^2$ so that the inside air temperature may cool down $2{\sim}3^{\circ}C$ on the basis of $35^{\circ}C$ atmospheric temperature in summer of south korean area.

Formation of Enzyme Resistant Starch by Extrusion Cooking of High Amylose Corn Starch (고아밀로즈 옥수수전분의 압출성형에 의한 난소화성화)

  • Kim, Ji-Yong;Lee, Cherl-Ho
    • Korean Journal of Food Science and Technology
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    • v.30 no.5
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    • pp.1128-1133
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    • 1998
  • Extrusion cooking treatment was compared with autoclaving/cooling treatment for formation of enzyme resistant starch of high amylose corn starch (HACS). Effects of barrel temperature $(100^{\circ}C,\;120^{\circ}C,\;140^{\circ}C)$ and feed moisture content (25%, 35%, 45%) on extrusion processing in a co-rotating twin-screw extruder under fixed screw speed (100 rpm) were investigated by measuring enzyme resistant starch (RS) yield. RS yield were estimated by in-vitro pancreatin digestion method and enzymatic-gravimetric method using termamyl. Barrel temperature and yield of RS were negatively correlated and feed moisture content and yield of RS was positively correlated as determined by in-vitro pancreatin method. The highest yield (38.4%) of RS was obtained from HACS extrudate processed at the barrel temperature of $100^{\circ}C$ and the feed moisture content of 45%, while the yield of RS by 5 times of autoclaving/cooling was 25%. The yield of RS by in vitro pancreatin digestion method was 20.7% with high amylose corn starch and 8.2% with ordinary corn starch (CS), respectively, under the same extrusion condition (barrel temperature $120^{\circ}C$, feed moisture content 35%). At the same condition, the yields of RS by enzyme-gravimetric method were 14.6% with HACS and 6.8% with CS, respectively. The yield of RS increased during the storage at $4^{\circ}C$ for 4 weeks and the highest yield (60%) was obtained by the storage of HACS extrudates extruded at $100^{\circ}C$ and 45% feed moisture content.

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Implementation of Linear Motor Piston Amplitude Estimator Using Phase Lag Filter (위상지연필터를 이용한 리니어 모터 피스톤 진폭 추정기의 구현)

  • Oh, Joon-Tae;Kim, Gyu-Sik
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.4
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    • pp.212-218
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    • 2013
  • In this paper, a linear motor piston amplitude estimator using phase lag filter has been implemented. In order to control the cooling capability of a refrigerator or an air conditioner in which liner compressors are applied, the piston speed should be controlled. The piston speed control can be obtained by adjusting the frequency or the stroke of linear motors. The dynamic performance of linear compressors depends on how accurately the stroke or the piston amplitude is estimated. A linear motor piston amplitude estimator using phase lag filter is proposed and the superior performance of our estimator is verified via some simulation studies.

A Numerical Study on the Effect of PCB Structure Variation on the Electronic Equipment Cooling (PCB 구조변화가 전자장비 냉각에 미치는 영향에 관한 수치적 연구)

  • ;;Park, Kyoung-Woo
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.19 no.12
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    • pp.3329-3343
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    • 1995
  • The interaction of mixed convection and surface radiation in a printed circuit board(PCB) is investigated numerically. The electronic equipment is modeled by a two-dimensional channel with three hot blocks. In order to calculate the turbulent flow characteristics, the low Reynolds number k-.epsilon. model which is proposed by Launder and Sharma is applied. The S-4 approximation is used to solve the radiative transfer equation. The effects of the Reynolds number and geometric configuration variation of PCB on the flow and heat transfer characteristics are analyzed. As the results of this study, it is found that the thermal boundary layer occured at adiabatic wall in case with thermal radiation included, and the effect of radiation is also found to be insignificant for high Reynolds numbers. It is found, as well, that the heat transfer increases as the Reynolds number and block space increase and the channel height decreases and the heat transfer of vertical channel is greater than that of horizontal channel.

Development of A Hoist Control Equipment for Shot Ball Transfer (쇼트볼 이송을 위한 호이스트 자동제어 장치 개발)

  • Choi, Jong-Jun;Choi, Young-Kiu
    • 전자공학회논문지 IE
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    • v.47 no.2
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    • pp.47-53
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    • 2010
  • The purpose of this paper is to build on automatic system for the shot ball transfer hoist. The shot ball is used to remove completely paint or leftovers before spreading the new paint on a large vessels surface. The shot ball is made of melted iron through cooling process, and it is transferred to hopper by electromagnet of hoist. Currently, the transfer process of the shot ball is performed by manual operation, and the transfer process is inefficient. So we have developed an automatic system to replace the manual system. The developed automation systems have efficient and accurate position control performance.

Analysis of aspheric and diffractive surface effect for long wavelength infrared lens (장파장 적외선 렌즈의 비구면 및 회절면 효과 분석)

  • 김현수;이동한;김현규;이국환
    • Korean Journal of Optics and Photonics
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    • v.14 no.4
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    • pp.369-376
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    • 2003
  • We analyzed the aspheric and/or diffractive surface effects to the performance in the long wavelength infrared (8-12 $\mu$m). Also we investigated the dependence of the NA values for the fixed effective focal length 100 mm when the field angle was varied from 5 degrees to 30 degrees stepped by 5 degrees. We chose the merit function as a criteria to compare the performance of the different lenses. Based on the analysis of the aspheric and/or diffractive surface effects, we designed the optical system of F/l.0 for the uncooled thermal imaging system. As for detector the pixel size was 45 $\mu$m square and the number of pixels were a 320${\times}$240 pixels.