• Journal of Practical Agriculture & Fisheries Research
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• v.23 no.2
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• pp.25-32
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• 2021

There is a need to breed and distribute of domestic varieties for Lilium longiflorum because most of the lily bulbs cultivated in Korea were imported from foreign country. The L. longiflorum 'Ace' and 'Nellie White' collected from 1996 had been self-crossed from 1996 to 2010. In 2012, the L. longiflorum 'Bright LoSong' was developed as a F₁ hybrid crossed between 'L₂-17-4' and 'L₂-16-6' that obtained by 7th self-fertilization. The 'Bright LoSong' was tested for homogeneity, regional adaptability test, consumer preference show from 2014 to 2015, and it was registered in 2017. The flower of 'Bright LoSong' is a trumpet shaped single flower with no spots.It has 5.8 flowers per plant, which is a relatively many number compared control 'Mount Carmel', Its flower diamater is 9.8cm, relatively a bit small size. And the flower inflorescence has umbel or raceme while the attitude of longitudinal axis is horizontal. The plant height is 38.4cm with 30.8 leaves per plant and 1.9cm of the leaf width. 'Bright LoSong' has a longer flowering period because of relatively small diameter or many number of flowers than those of the control 'Mount Carmel'. Thus, the 'Bright LoSong' showed a high consumer preference, and the cultivar has the advantage of being able to reproduce since the parents are pure lines.

• FLOWER RESEARCH JOURNAL
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• v.16 no.4
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• pp.304-308
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• 2008

Lilium longiflorum 'Charm' as a $F_1$ hybrid cultivar was released by crossing inbred line '$L_2$-14' and '$L_2$-21' which were obtained from 5 self crosses originated from 'Nellie White', 'Ace' and 'Hinomoto'. The growth and flowering characteristics were evaluated in a greenhouse maintained at a minimum of $13^{\circ}C$ at night during winter in 2006 and 2007. The flower of 'Charm' is white color and horizontal-facing. The flower number of a plant and its diameter is 7.4 and 16.5 cm with 24.5 ornamental(flowering) days. The plant height is 60.3 cm with 70.3 number of leaves. The stem diameter and internode length is 1.2 cm and 1.1 cm, respectively, meaning the plant is compact and sturdy. And the number of seed per a capsule is 251.1. The results of these evaluation, therefore, suggest that seedling Lilium longiflorum 'Charm' can be used as a pot plant due to its short stems, many number of flowers per plant, long ornamental period, strong growth habit with many leaves and thick stem diameter.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.328-331
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• 2009

The white flowered 'Eorayeon 2ho' is a cultivar of Lilium ${\times}$ formolonga developed in Department of Horticultural Sciences, Kangwon National University, Chuncheon, Korea. $F_1$ hybrid LA-77 was selected from the cross between a selected selting line($S_3$) of L. longiflorum 'Lorina' and a selfing line($S_3$) of L. ${\times}$ formolonga 'Augusta' in 2000. The $F_1$ hybrid LA-77 was backcrossed by a selected line of L. ${\times}$ far molonga 'Raizan No. 1' from 2001 to 2002. 'MF-No.2' was preliminarily selected for its upward flowering habit and high plant height. The evaluation of characteristics was investigated from 2003 to 2004 in Chuncheon and Yungweol, Kangwon province. 'Eorayeon 2ho' blooms in early July at outside, with high resistance to Botrytis and showing upward flowering habit. The plant height is 132.6 cm and the percentage of seed germination is 95.3%. Mean tepal length and width are 16.0 em and 4.3 cm, respectively. The length and width of leaf is 12.3 and 3.2 cm, respectively. This seed propagating cultivar shows high plant height suitable for a cut flower.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.324-327
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• 2009

The white flowered 'Eorayeon 1ho' is the first Kangwon National University cultivar of L. ${\times}$ formolonga, commonly known as seed propagating lily. Crosses were conducted from 1999 to 2003 among the selected selfing lines($S_3$) of L. longiflorum 'Lorina', L. ${\times}$ formolonga 'Augusta' and 'Raizan Herald'. A new cultivar was preliminarily selected as 'E-No.1' in 2003 for its stable upward flowering habit. The evaluation of characteristics was investigated from 2003 to 2004 in Chuncheon and Yungweol, Kangwon province. 'Eorayeon 1ho' blooms in early July at outside, with high resistance to Botrytis and having upward flowering habit. The plant height is 112.4 cm and the percentage of seed germination is 96.4%. This seed propagating cultivar shows good quality as a cut flower and low blasting rate.

• Horticultural Science & Technology
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• v.19 no.3
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• pp.373-377
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• 2001

This study was conducted to find optimum pollination methods for overcoming pre-fertilization incompatibility in interspecific crosses between L. longiflorum 'Gelria' (LG) and L. cernuum (LC). In cross combination of $LC{\times}LG$, the survival rate of the ovaries was higher in the cut style pollinations than in normal stigmatic pollination. Among the various cut style pollination methods, pollination after cutting the styles 2 3mm length above the ovary and top of the ovary were the most effective ones. No ovaries and ovules survived in cross pollination of $LC{\times}LG$. In self-pollination of LG and cross pollination of $LC{\times}LG$, pollens germinated on the stigma by 12 hours and reached to the ovules by 90 hours after pollination. However, in the cross pollination of $LC{\times}LG$, pollen tubes elongated very slowly until 48 hours after pollination and a lot of deformed tubes were observed in the styler canal having swelled tube tip and irregular heavy callose deposition. The pollination time after anthesis also affected on the survival rate of ovaries and ovules showing the best result when pollinated on three days after anthesis. Stigma exudate promoted slightly the enlargement of ovaries, but the survival rate of ovaries and ovules was not increased.

• Korean Journal of Plant Tissue Culture
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• v.27 no.1
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• pp.25-29
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• 2000

Liquid shaking culture was conducted to investigate the proper culture conditions for the micropropagation of high quality lily using bulblets (3 mm in diameter) obtained from small scale culture. The combinations of 9% sucrose and 10 mM nitrogen or 6% sucrose and 20 mM $NH_4NO_3$ were effective on the growth and weight of micro-bulbs. However, the number of new bulbs was the highest when 20 to 40 mM $NH_4NO_3$ and 3% sucrose were added to the MS medium. The total fresh weight was increased effectively in MS medium supplemented with BA 0.2 mg/L alone under $60\;{\mu}mol{\cdot}m^2{\cdot}sec^{-1}$ intensity. Also bulblet weight was increased at $60\;{\mu}mol{\cdot}m^2{\cdot}sec^{-1}$ intensity, regardless of BA concentrations (0.2 and 2 mg/L) in the medium. The proper culture period of bulblet was about 2 month in liquid shaking culture.

• Horticultural Science & Technology
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• v.19 no.3
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• pp.378-383
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• 2001

Embryo culture, ovule culture and ovary slice culture were tested to find optimum method for overcoming post fertilization barrier in interspecific crosses between L. longiflorum 'Gelria' and L. cernuum. Although reciprocal crosses between the species were carried out by cut-style pollination method, fruits developed only in crosses of L. longiflorum${\times}$L. cernuum. On the 40 days after pollination, ovaries were sliced into 2-4mm thickness and cultured on a hormone-free Murashige-Skoog (MS) medium, supplemented with 2%, 4%, 6%, 8% and 10% sucrose. For the L. longiflorum Gelria'${\times}$L. cernuum cross, ovule development was found to be best at 6% sucrose and a lot of hybrid plant lets established directly from the ovary slice culture and subsequent ovule culture. High concentration of sucrose above 8% made ovules abort or vitrificate from 40 days after culture. In contrast, ovules from the L. cernuum${\times}$L. longiflorum 'Gelria' cross swelled well in ovary slice culture, however, they did not germinated in subsequent ovule culture. On the 60 days after pollination, ovules thicker than 0.6mm was interpreted as one containing embryo. The embryo size ranged from 1.2 mm to 1.7 mm, and in vitro development of the excised embryos was found to be best with the MS medium (pH 5.8), supplemented with $0.1-1 mg{\cdot}L^{-1}$ NAA and 6% sucrose. Thick ovules excised 60 days after pollination germinated about 60% as normal seeds in MS medium supplemented with 6% sucrose and free hormone. The ovule culture 60 days after pollination was concluded to be most recommendable to produce interspecific hybrids in large scale crosses between L. longiflorum 'Gelria' and L. cernuum by the reason of easy procedure.